Intramyocardial gene silencing by interfering RNA

Autores
Brea, María Soledad; Morgan, Patricio Eduardo; Perez, Nestor Gustavo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
RNAi is a widely used methodology for gene silencing. The action mechanism of siRNA molecules has been well studiedin recent years, and the technique has been optimized in terms of safety and effectiveness. Cardiovascular diseases havea high incidence in the current population, and despite of the extensive research, safe and efficient therapeutics have notyet been found, which is reflected by 17.1 million people who die each year for this cause. In this context, siRNAs arebeing considered a therapeutic tool to regulate the expression of genes involved in the generation of these pathologies.The efficacy of siRNAs entry to cardiomyocytes, the safety of the delivery process and the degree of silencing achievedare main aspects before consider it as a cardiovascular disease therapy. Presently, we will give a brief outline of thecurrent understanding of the RNAi mechanism and the delivery system to the heart. We describe the use of lentivirus fora functional silencing of cardiac proteins in the study of a pathophysiological process, the slow force response to cardiacstretch.
Fil: Brea, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Fil: Morgan, Patricio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Fil: Perez, Nestor Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Materia
SIRNA
HEART
LENTIVIRUS
CARDIOMYOCYTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49492
Acceder
id CONICETDig_8014b74ade3b50a9afa8af9b8e48b72d
oai_identifier_str oai:ri.conicet.gov.ar:11336/49492
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intramyocardial gene silencing by interfering RNABrea, María SoledadMorgan, Patricio EduardoPerez, Nestor GustavoSIRNAHEARTLENTIVIRUSCARDIOMYOCYTEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3RNAi is a widely used methodology for gene silencing. The action mechanism of siRNA molecules has been well studiedin recent years, and the technique has been optimized in terms of safety and effectiveness. Cardiovascular diseases havea high incidence in the current population, and despite of the extensive research, safe and efficient therapeutics have notyet been found, which is reflected by 17.1 million people who die each year for this cause. In this context, siRNAs arebeing considered a therapeutic tool to regulate the expression of genes involved in the generation of these pathologies.The efficacy of siRNAs entry to cardiomyocytes, the safety of the delivery process and the degree of silencing achievedare main aspects before consider it as a cardiovascular disease therapy. Presently, we will give a brief outline of thecurrent understanding of the RNAi mechanism and the delivery system to the heart. We describe the use of lentivirus fora functional silencing of cardiac proteins in the study of a pathophysiological process, the slow force response to cardiacstretch.Fil: Brea, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; ArgentinaFil: Morgan, Patricio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; ArgentinaFil: Perez, Nestor Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; ArgentinaAllied Academies2017-06-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49492Brea, María Soledad; Morgan, Patricio Eduardo; Perez, Nestor Gustavo; Intramyocardial gene silencing by interfering RNA; Allied Academies; Journal of RNA and Genomics; 13; 1; 12-6-2017; 550-5561747-0854CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.alliedacademies.org/articles/intramyocardial-gene-silencing-by-interfering-rna-7644.htinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:32Zoai:ri.conicet.gov.ar:11336/49492instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:32.28CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intramyocardial gene silencing by interfering RNA
title Intramyocardial gene silencing by interfering RNA
spellingShingle Intramyocardial gene silencing by interfering RNA
Brea, María Soledad
SIRNA
HEART
LENTIVIRUS
CARDIOMYOCYTE
title_short Intramyocardial gene silencing by interfering RNA
title_full Intramyocardial gene silencing by interfering RNA
title_fullStr Intramyocardial gene silencing by interfering RNA
title_full_unstemmed Intramyocardial gene silencing by interfering RNA
title_sort Intramyocardial gene silencing by interfering RNA
dc.creator.none.fl_str_mv Brea, María Soledad
Morgan, Patricio Eduardo
Perez, Nestor Gustavo
author Brea, María Soledad
author_facet Brea, María Soledad
Morgan, Patricio Eduardo
Perez, Nestor Gustavo
author_role author
author2 Morgan, Patricio Eduardo
Perez, Nestor Gustavo
author2_role author
author
dc.subject.none.fl_str_mv SIRNA
HEART
LENTIVIRUS
CARDIOMYOCYTE
topic SIRNA
HEART
LENTIVIRUS
CARDIOMYOCYTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv RNAi is a widely used methodology for gene silencing. The action mechanism of siRNA molecules has been well studiedin recent years, and the technique has been optimized in terms of safety and effectiveness. Cardiovascular diseases havea high incidence in the current population, and despite of the extensive research, safe and efficient therapeutics have notyet been found, which is reflected by 17.1 million people who die each year for this cause. In this context, siRNAs arebeing considered a therapeutic tool to regulate the expression of genes involved in the generation of these pathologies.The efficacy of siRNAs entry to cardiomyocytes, the safety of the delivery process and the degree of silencing achievedare main aspects before consider it as a cardiovascular disease therapy. Presently, we will give a brief outline of thecurrent understanding of the RNAi mechanism and the delivery system to the heart. We describe the use of lentivirus fora functional silencing of cardiac proteins in the study of a pathophysiological process, the slow force response to cardiacstretch.
Fil: Brea, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Fil: Morgan, Patricio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Fil: Perez, Nestor Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
description RNAi is a widely used methodology for gene silencing. The action mechanism of siRNA molecules has been well studiedin recent years, and the technique has been optimized in terms of safety and effectiveness. Cardiovascular diseases havea high incidence in the current population, and despite of the extensive research, safe and efficient therapeutics have notyet been found, which is reflected by 17.1 million people who die each year for this cause. In this context, siRNAs arebeing considered a therapeutic tool to regulate the expression of genes involved in the generation of these pathologies.The efficacy of siRNAs entry to cardiomyocytes, the safety of the delivery process and the degree of silencing achievedare main aspects before consider it as a cardiovascular disease therapy. Presently, we will give a brief outline of thecurrent understanding of the RNAi mechanism and the delivery system to the heart. We describe the use of lentivirus fora functional silencing of cardiac proteins in the study of a pathophysiological process, the slow force response to cardiacstretch.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49492
Brea, María Soledad; Morgan, Patricio Eduardo; Perez, Nestor Gustavo; Intramyocardial gene silencing by interfering RNA; Allied Academies; Journal of RNA and Genomics; 13; 1; 12-6-2017; 550-556
1747-0854
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49492
identifier_str_mv Brea, María Soledad; Morgan, Patricio Eduardo; Perez, Nestor Gustavo; Intramyocardial gene silencing by interfering RNA; Allied Academies; Journal of RNA and Genomics; 13; 1; 12-6-2017; 550-556
1747-0854
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.alliedacademies.org/articles/intramyocardial-gene-silencing-by-interfering-rna-7644.ht
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Allied Academies
publisher.none.fl_str_mv Allied Academies
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268608375291904
score 13.13397

Publicaciones similares