The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata

Autores
Guiñazu, Lorena Belen; Pastor, Nicolás Alejandro; Autran, Valeria Alejandra; Cejas, Leonardo Gabriel; Torres, Adriana Mabel; Andrés, Javier Alberto
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The high moisture content and water-soluble nutrients in tomato fruits make them perishable and susceptible to a number of fungal pathogens that cause postharvest rots. The Alternaria genus, widely distributed in nature, includes pathogenic species that can infect field crops or cause significant post-harvest damage, behaving as a facultative pathogen that is favored by stress, maturity, and senescence of the host. In the tomato fruit, the conidia of the fungus germinate and penetrate through wounds and the infection remains latent until maturity when tissues weaken. A. alternata infection is visualized as dark brown to black, smooth, slightly sunken, firm-textured lesions that can reach several centimeters in diameter. The application of fungicides is a common strategy used in an attempt to minimize post-harvest losses; however, this practice has caused environmental problems due to its residual toxicity, stimulated the appearance of strains resistant to active principles, and generated concern for human and animal safety. A sustainable alternative is the development of products based on biological control agents. Among them, bacteria of the Pseudomonas genus have been reported as efficient against various fungi. In this work, the effectiveness of Pseudomonas sp. PCI2 in suppressing diseases caused by A. alternata in tomato fruits was evaluated. To establish the antagonistic activity of the bacterial strain against the fungal strain, commercially ripe tomato fruits were superficially disinfected by immersion in a suspension of 2% sodium hypochlorite for 2 min, rinsed with sterile distilled water, and then they were dried by a stream of sterile air in a laminar flow chamber. Incision wounds were made on the fruits, 3 mm deep and 3 mm in diameter in the equatorial region. Immediately, 20 µL of an aqueous suspension of Pseudomonas sp. PCI2 were applied to each of the wounds, evaluating different concentrations (109 , 107 , and 105 CFU/mL). Three hours later, 15 µL of a suspension containing 104conidia/mL of A. alternata was applied to each wound. The fruits were kept in a chamber at 20ºC and 95% humidity for 7 days in plastic containers protected with plastic wrap. Inoculation with the Pseudomonas sp. PCI2 strain showed a significant decrease in the symptoms of the disease, with an average reduction of 50% in the area of the lesions, a result that was observed with all bacterial concentrations, suggesting the use of the lowest. Therefore, this microorganism can be considered a promising tool in the biological control of A. alternata and suitable for the design of an effective strategy for the conservation of fruits.
Fil: Guiñazu, Lorena Belen. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Pastor, Nicolás Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Autran, Valeria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Cejas, Leonardo Gabriel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Andrés, Javier Alberto. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
XL Annual Meeting of Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Materia
Pseudomonas PCI2
Post harvest deterioration
Tomato
Alternaria alternata
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/273742
Acceder
id CONICETDig_8e08282a7036d7206ce85113aaa73313
oai_identifier_str oai:ri.conicet.gov.ar:11336/273742
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternataGuiñazu, Lorena BelenPastor, Nicolás AlejandroAutran, Valeria AlejandraCejas, Leonardo GabrielTorres, Adriana MabelAndrés, Javier AlbertoPseudomonas PCI2Post harvest deteriorationTomatoAlternaria alternatahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The high moisture content and water-soluble nutrients in tomato fruits make them perishable and susceptible to a number of fungal pathogens that cause postharvest rots. The Alternaria genus, widely distributed in nature, includes pathogenic species that can infect field crops or cause significant post-harvest damage, behaving as a facultative pathogen that is favored by stress, maturity, and senescence of the host. In the tomato fruit, the conidia of the fungus germinate and penetrate through wounds and the infection remains latent until maturity when tissues weaken. A. alternata infection is visualized as dark brown to black, smooth, slightly sunken, firm-textured lesions that can reach several centimeters in diameter. The application of fungicides is a common strategy used in an attempt to minimize post-harvest losses; however, this practice has caused environmental problems due to its residual toxicity, stimulated the appearance of strains resistant to active principles, and generated concern for human and animal safety. A sustainable alternative is the development of products based on biological control agents. Among them, bacteria of the Pseudomonas genus have been reported as efficient against various fungi. In this work, the effectiveness of Pseudomonas sp. PCI2 in suppressing diseases caused by A. alternata in tomato fruits was evaluated. To establish the antagonistic activity of the bacterial strain against the fungal strain, commercially ripe tomato fruits were superficially disinfected by immersion in a suspension of 2% sodium hypochlorite for 2 min, rinsed with sterile distilled water, and then they were dried by a stream of sterile air in a laminar flow chamber. Incision wounds were made on the fruits, 3 mm deep and 3 mm in diameter in the equatorial region. Immediately, 20 µL of an aqueous suspension of Pseudomonas sp. PCI2 were applied to each of the wounds, evaluating different concentrations (109 , 107 , and 105 CFU/mL). Three hours later, 15 µL of a suspension containing 104conidia/mL of A. alternata was applied to each wound. The fruits were kept in a chamber at 20ºC and 95% humidity for 7 days in plastic containers protected with plastic wrap. Inoculation with the Pseudomonas sp. PCI2 strain showed a significant decrease in the symptoms of the disease, with an average reduction of 50% in the area of the lesions, a result that was observed with all bacterial concentrations, suggesting the use of the lowest. Therefore, this microorganism can be considered a promising tool in the biological control of A. alternata and suitable for the design of an effective strategy for the conservation of fruits.Fil: Guiñazu, Lorena Belen. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Pastor, Nicolás Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Autran, Valeria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Cejas, Leonardo Gabriel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Andrés, Javier Alberto. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaXL Annual Meeting of Sociedad de Biología de CuyoMendozaArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/273742The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata; XL Annual Meeting of Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 5-51667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.4/55011/pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:07:17Zoai:ri.conicet.gov.ar:11336/273742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:07:18.206CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata
title The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata
spellingShingle The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata
Guiñazu, Lorena Belen
Pseudomonas PCI2
Post harvest deterioration
Tomato
Alternaria alternata
title_short The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata
title_full The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata
title_fullStr The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata
title_full_unstemmed The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata
title_sort The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata
dc.creator.none.fl_str_mv Guiñazu, Lorena Belen
Pastor, Nicolás Alejandro
Autran, Valeria Alejandra
Cejas, Leonardo Gabriel
Torres, Adriana Mabel
Andrés, Javier Alberto
author Guiñazu, Lorena Belen
author_facet Guiñazu, Lorena Belen
Pastor, Nicolás Alejandro
Autran, Valeria Alejandra
Cejas, Leonardo Gabriel
Torres, Adriana Mabel
Andrés, Javier Alberto
author_role author
author2 Pastor, Nicolás Alejandro
Autran, Valeria Alejandra
Cejas, Leonardo Gabriel
Torres, Adriana Mabel
Andrés, Javier Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Pseudomonas PCI2
Post harvest deterioration
Tomato
Alternaria alternata
topic Pseudomonas PCI2
Post harvest deterioration
Tomato
Alternaria alternata
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The high moisture content and water-soluble nutrients in tomato fruits make them perishable and susceptible to a number of fungal pathogens that cause postharvest rots. The Alternaria genus, widely distributed in nature, includes pathogenic species that can infect field crops or cause significant post-harvest damage, behaving as a facultative pathogen that is favored by stress, maturity, and senescence of the host. In the tomato fruit, the conidia of the fungus germinate and penetrate through wounds and the infection remains latent until maturity when tissues weaken. A. alternata infection is visualized as dark brown to black, smooth, slightly sunken, firm-textured lesions that can reach several centimeters in diameter. The application of fungicides is a common strategy used in an attempt to minimize post-harvest losses; however, this practice has caused environmental problems due to its residual toxicity, stimulated the appearance of strains resistant to active principles, and generated concern for human and animal safety. A sustainable alternative is the development of products based on biological control agents. Among them, bacteria of the Pseudomonas genus have been reported as efficient against various fungi. In this work, the effectiveness of Pseudomonas sp. PCI2 in suppressing diseases caused by A. alternata in tomato fruits was evaluated. To establish the antagonistic activity of the bacterial strain against the fungal strain, commercially ripe tomato fruits were superficially disinfected by immersion in a suspension of 2% sodium hypochlorite for 2 min, rinsed with sterile distilled water, and then they were dried by a stream of sterile air in a laminar flow chamber. Incision wounds were made on the fruits, 3 mm deep and 3 mm in diameter in the equatorial region. Immediately, 20 µL of an aqueous suspension of Pseudomonas sp. PCI2 were applied to each of the wounds, evaluating different concentrations (109 , 107 , and 105 CFU/mL). Three hours later, 15 µL of a suspension containing 104conidia/mL of A. alternata was applied to each wound. The fruits were kept in a chamber at 20ºC and 95% humidity for 7 days in plastic containers protected with plastic wrap. Inoculation with the Pseudomonas sp. PCI2 strain showed a significant decrease in the symptoms of the disease, with an average reduction of 50% in the area of the lesions, a result that was observed with all bacterial concentrations, suggesting the use of the lowest. Therefore, this microorganism can be considered a promising tool in the biological control of A. alternata and suitable for the design of an effective strategy for the conservation of fruits.
Fil: Guiñazu, Lorena Belen. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Pastor, Nicolás Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Autran, Valeria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Cejas, Leonardo Gabriel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Andrés, Javier Alberto. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
XL Annual Meeting of Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
description The high moisture content and water-soluble nutrients in tomato fruits make them perishable and susceptible to a number of fungal pathogens that cause postharvest rots. The Alternaria genus, widely distributed in nature, includes pathogenic species that can infect field crops or cause significant post-harvest damage, behaving as a facultative pathogen that is favored by stress, maturity, and senescence of the host. In the tomato fruit, the conidia of the fungus germinate and penetrate through wounds and the infection remains latent until maturity when tissues weaken. A. alternata infection is visualized as dark brown to black, smooth, slightly sunken, firm-textured lesions that can reach several centimeters in diameter. The application of fungicides is a common strategy used in an attempt to minimize post-harvest losses; however, this practice has caused environmental problems due to its residual toxicity, stimulated the appearance of strains resistant to active principles, and generated concern for human and animal safety. A sustainable alternative is the development of products based on biological control agents. Among them, bacteria of the Pseudomonas genus have been reported as efficient against various fungi. In this work, the effectiveness of Pseudomonas sp. PCI2 in suppressing diseases caused by A. alternata in tomato fruits was evaluated. To establish the antagonistic activity of the bacterial strain against the fungal strain, commercially ripe tomato fruits were superficially disinfected by immersion in a suspension of 2% sodium hypochlorite for 2 min, rinsed with sterile distilled water, and then they were dried by a stream of sterile air in a laminar flow chamber. Incision wounds were made on the fruits, 3 mm deep and 3 mm in diameter in the equatorial region. Immediately, 20 µL of an aqueous suspension of Pseudomonas sp. PCI2 were applied to each of the wounds, evaluating different concentrations (109 , 107 , and 105 CFU/mL). Three hours later, 15 µL of a suspension containing 104conidia/mL of A. alternata was applied to each wound. The fruits were kept in a chamber at 20ºC and 95% humidity for 7 days in plastic containers protected with plastic wrap. Inoculation with the Pseudomonas sp. PCI2 strain showed a significant decrease in the symptoms of the disease, with an average reduction of 50% in the area of the lesions, a result that was observed with all bacterial concentrations, suggesting the use of the lowest. Therefore, this microorganism can be considered a promising tool in the biological control of A. alternata and suitable for the design of an effective strategy for the conservation of fruits.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/273742
The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata; XL Annual Meeting of Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 5-5
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/273742
identifier_str_mv The protective role of Pseudomonas spp. PCI2 on post harvest deterioration of tomato caused by Alternaria alternata; XL Annual Meeting of Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 5-5
1667-5746
CONICET Digital
CONICET
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language eng
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dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
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