Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (...

Autores
Pedersen, Lasse E.; Patch, Jared R.; Kenney, Mary; Glabman, Raisa A.; Nielsen, Morten; Jungersen, Gregers; Buus, Soren; Golde, William T.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The immune response to the highly acute foot-and-mouth disease virus (FMDV) is routinely reported as a measure of serum antibody. However, a critical effector function of immune responses combating viral infection of mammals is the cytotoxic T lymphocyte (CTL) response mediated by virus specific CD8 expressing T cells. This immune mechanism arrests viral spread by killing virus infected cells before new, mature virus can develop. We have previously shown that infection of swine by FMDV results in a measurable CTL response and have correlated CTL killing of virus-infected cells with specific class I major histocompatibility complex (MHC) tetramer staining. We also showed that a modified replication defective human adenovirus 5 vector expressing the FMDV structural proteins (Ad5-FMDV-T vaccine) targets the induction of a CD8+ CTL response with a minimal humoral response. In this report, we show that the specificity of the CD8+ T cell response to Ad5-FMDV-T varies between cohorts of genetically identical animals. Further, we demonstrate epitope specificity of CD8+ T cells expands following multiple immunizations with this vaccine.
Fil: Pedersen, Lasse E.. United States Department of Agriculture. Agricultural Research Service; Argentina. Technical University of Denmark; Dinamarca
Fil: Patch, Jared R.. United States Department of Agriculture. Agricultural Research Service; Argentina. University of Vermont; Estados Unidos
Fil: Kenney, Mary. United States Department of Agriculture. Agricultural Research Service; Argentina
Fil: Glabman, Raisa A.. United States Department of Agriculture. Agricultural Research Service; Argentina. University of Vermont; Estados Unidos
Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Jungersen, Gregers. Technical University of Denmark; Dinamarca
Fil: Buus, Soren. Universidad de Copenhagen; Dinamarca
Fil: Golde, William T.. United States Department of Agriculture. Agricultural Research Service; Argentina
Materia
Class I
Cytotoxic T Lymphocytes
Mhc
Mhc Tetramers
Sla
T Cell Epitopes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48612
Acceder
id CONICETDig_8d7b149d85f4945dec056b9886cb6dd5
oai_identifier_str oai:ri.conicet.gov.ar:11336/48612
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccinePedersen, Lasse E.Patch, Jared R.Kenney, MaryGlabman, Raisa A.Nielsen, MortenJungersen, GregersBuus, SorenGolde, William T.Class ICytotoxic T LymphocytesMhcMhc TetramersSlaT Cell Epitopeshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The immune response to the highly acute foot-and-mouth disease virus (FMDV) is routinely reported as a measure of serum antibody. However, a critical effector function of immune responses combating viral infection of mammals is the cytotoxic T lymphocyte (CTL) response mediated by virus specific CD8 expressing T cells. This immune mechanism arrests viral spread by killing virus infected cells before new, mature virus can develop. We have previously shown that infection of swine by FMDV results in a measurable CTL response and have correlated CTL killing of virus-infected cells with specific class I major histocompatibility complex (MHC) tetramer staining. We also showed that a modified replication defective human adenovirus 5 vector expressing the FMDV structural proteins (Ad5-FMDV-T vaccine) targets the induction of a CD8+ CTL response with a minimal humoral response. In this report, we show that the specificity of the CD8+ T cell response to Ad5-FMDV-T varies between cohorts of genetically identical animals. Further, we demonstrate epitope specificity of CD8+ T cells expands following multiple immunizations with this vaccine.Fil: Pedersen, Lasse E.. United States Department of Agriculture. Agricultural Research Service; Argentina. Technical University of Denmark; DinamarcaFil: Patch, Jared R.. United States Department of Agriculture. Agricultural Research Service; Argentina. University of Vermont; Estados UnidosFil: Kenney, Mary. United States Department of Agriculture. Agricultural Research Service; ArgentinaFil: Glabman, Raisa A.. United States Department of Agriculture. Agricultural Research Service; Argentina. University of Vermont; Estados UnidosFil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Jungersen, Gregers. Technical University of Denmark; DinamarcaFil: Buus, Soren. Universidad de Copenhagen; DinamarcaFil: Golde, William T.. United States Department of Agriculture. Agricultural Research Service; ArgentinaElsevier Science2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48612Pedersen, Lasse E.; Patch, Jared R.; Kenney, Mary; Glabman, Raisa A.; Nielsen, Morten; et al.; Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine; Elsevier Science; Veterinary Immunology And Immunopathology; 181; 11-2016; 59-670165-2427CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2016.07.012info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165242716301404info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:09Zoai:ri.conicet.gov.ar:11336/48612instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:10.019CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine
title Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine
spellingShingle Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine
Pedersen, Lasse E.
Class I
Cytotoxic T Lymphocytes
Mhc
Mhc Tetramers
Sla
T Cell Epitopes
title_short Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine
title_full Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine
title_fullStr Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine
title_full_unstemmed Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine
title_sort Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine
dc.creator.none.fl_str_mv Pedersen, Lasse E.
Patch, Jared R.
Kenney, Mary
Glabman, Raisa A.
Nielsen, Morten
Jungersen, Gregers
Buus, Soren
Golde, William T.
author Pedersen, Lasse E.
author_facet Pedersen, Lasse E.
Patch, Jared R.
Kenney, Mary
Glabman, Raisa A.
Nielsen, Morten
Jungersen, Gregers
Buus, Soren
Golde, William T.
author_role author
author2 Patch, Jared R.
Kenney, Mary
Glabman, Raisa A.
Nielsen, Morten
Jungersen, Gregers
Buus, Soren
Golde, William T.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Class I
Cytotoxic T Lymphocytes
Mhc
Mhc Tetramers
Sla
T Cell Epitopes
topic Class I
Cytotoxic T Lymphocytes
Mhc
Mhc Tetramers
Sla
T Cell Epitopes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The immune response to the highly acute foot-and-mouth disease virus (FMDV) is routinely reported as a measure of serum antibody. However, a critical effector function of immune responses combating viral infection of mammals is the cytotoxic T lymphocyte (CTL) response mediated by virus specific CD8 expressing T cells. This immune mechanism arrests viral spread by killing virus infected cells before new, mature virus can develop. We have previously shown that infection of swine by FMDV results in a measurable CTL response and have correlated CTL killing of virus-infected cells with specific class I major histocompatibility complex (MHC) tetramer staining. We also showed that a modified replication defective human adenovirus 5 vector expressing the FMDV structural proteins (Ad5-FMDV-T vaccine) targets the induction of a CD8+ CTL response with a minimal humoral response. In this report, we show that the specificity of the CD8+ T cell response to Ad5-FMDV-T varies between cohorts of genetically identical animals. Further, we demonstrate epitope specificity of CD8+ T cells expands following multiple immunizations with this vaccine.
Fil: Pedersen, Lasse E.. United States Department of Agriculture. Agricultural Research Service; Argentina. Technical University of Denmark; Dinamarca
Fil: Patch, Jared R.. United States Department of Agriculture. Agricultural Research Service; Argentina. University of Vermont; Estados Unidos
Fil: Kenney, Mary. United States Department of Agriculture. Agricultural Research Service; Argentina
Fil: Glabman, Raisa A.. United States Department of Agriculture. Agricultural Research Service; Argentina. University of Vermont; Estados Unidos
Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Jungersen, Gregers. Technical University of Denmark; Dinamarca
Fil: Buus, Soren. Universidad de Copenhagen; Dinamarca
Fil: Golde, William T.. United States Department of Agriculture. Agricultural Research Service; Argentina
description The immune response to the highly acute foot-and-mouth disease virus (FMDV) is routinely reported as a measure of serum antibody. However, a critical effector function of immune responses combating viral infection of mammals is the cytotoxic T lymphocyte (CTL) response mediated by virus specific CD8 expressing T cells. This immune mechanism arrests viral spread by killing virus infected cells before new, mature virus can develop. We have previously shown that infection of swine by FMDV results in a measurable CTL response and have correlated CTL killing of virus-infected cells with specific class I major histocompatibility complex (MHC) tetramer staining. We also showed that a modified replication defective human adenovirus 5 vector expressing the FMDV structural proteins (Ad5-FMDV-T vaccine) targets the induction of a CD8+ CTL response with a minimal humoral response. In this report, we show that the specificity of the CD8+ T cell response to Ad5-FMDV-T varies between cohorts of genetically identical animals. Further, we demonstrate epitope specificity of CD8+ T cells expands following multiple immunizations with this vaccine.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48612
Pedersen, Lasse E.; Patch, Jared R.; Kenney, Mary; Glabman, Raisa A.; Nielsen, Morten; et al.; Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine; Elsevier Science; Veterinary Immunology And Immunopathology; 181; 11-2016; 59-67
0165-2427
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48612
identifier_str_mv Pedersen, Lasse E.; Patch, Jared R.; Kenney, Mary; Glabman, Raisa A.; Nielsen, Morten; et al.; Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine; Elsevier Science; Veterinary Immunology And Immunopathology; 181; 11-2016; 59-67
0165-2427
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2016.07.012
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165242716301404
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614103879909376
score 13.070432

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