Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs

Autores
Valle, María Ivone; Flamini, Daniel Omar; Sandoval, Marisa Julia; Saidman, Silvana Beatriz; Massheimer, Virginia Laura
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Hemostasis plays an active role in atherosclerotic disease and arterial stenosis. Nitinol (NiTi) is used in several biomedical applications such as stents. Despite its biocompatibility, elasticity and corrosion resistance, some Ni2+ and Ti2+ ions may be released, resulting in local and systemic unwanted effects. The use of polypyrrole (PPy) coating, protects from ion release and, allows doping with drugs designed such as heparin (Hep) and sodium salicylate (NaSa). This work evaluates the effect on blood coagulation and platelet aggregation (PA) of NiTi-PPy devices (slices of 1cm2) doped with Hep (0.2; 0.3; 0.4 g/L), NaSa (0.1 and 0.5 M) or the combination of NaSa plus Hep (0.5M + 0.2 g/L). The slices were incubated 35 min with human plasma, and immediately after aliquots were taken for coagulation tests. Platelet poor plasma (PPP) was used for thrombin time (TT) and fibrinogen (F) measurements, and platelet rich plasma (PRP) for PA assays. TT was prolonged by Hep device. The anticoagulant effect was proportional to Hep concentration (25 - >120 sec; 0.2 to 0.4 g/L). F content was unchanged compared to PPP (316±16.5 vs 322±35.7 mg/dL). The 0.1 M NaSa slice inhibited PA (42% IPA). TT time was enlarged (34.8±9.9 sec.), F content decreased (15% vs PPP) and IAP was higher (90% IAP, p<0.05) using 0.5 M NaSa. Both drugs doping showed an anticoagulant additive effect (TT: 60±20.5 sec.) with significant F diminution (49% vs PPP). Scanning electron microscopy showed that slices that contained 0.5 M NaSa or NaSa plus Hep, exhibited a complex microtubular structure. The anticoagulant effect of NaSa slices could be due to the hiding of F in these three-dimensional structures. Platelet deposition was rule out since platelet counting in the remaining PRP was not changed respect to basal count. The results suggest that, these devices are able to produce an in situ beneficial regulation of hemostasis, and could represent a potential design to prevent restenosis of blood vessels.
Fil: Valle, María Ivone. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Flamini, Daniel Omar. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Sandoval, Marisa Julia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Saidman, Silvana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; Argentina
Fil: Massheimer, Virginia Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual Sociedad Argentina de Inmunología y XLVIII Reunión Anual Sociedad Argentina de Farmacología Experimental
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Farmacología Experimental
Materia
HEMOSTASIS
NITINOL
HEPARIN
ANTICOAGULANT ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/235551
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugsValle, María IvoneFlamini, Daniel OmarSandoval, Marisa JuliaSaidman, Silvana BeatrizMassheimer, Virginia LauraHEMOSTASISNITINOLHEPARINANTICOAGULANT ACTIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hemostasis plays an active role in atherosclerotic disease and arterial stenosis. Nitinol (NiTi) is used in several biomedical applications such as stents. Despite its biocompatibility, elasticity and corrosion resistance, some Ni2+ and Ti2+ ions may be released, resulting in local and systemic unwanted effects. The use of polypyrrole (PPy) coating, protects from ion release and, allows doping with drugs designed such as heparin (Hep) and sodium salicylate (NaSa). This work evaluates the effect on blood coagulation and platelet aggregation (PA) of NiTi-PPy devices (slices of 1cm2) doped with Hep (0.2; 0.3; 0.4 g/L), NaSa (0.1 and 0.5 M) or the combination of NaSa plus Hep (0.5M + 0.2 g/L). The slices were incubated 35 min with human plasma, and immediately after aliquots were taken for coagulation tests. Platelet poor plasma (PPP) was used for thrombin time (TT) and fibrinogen (F) measurements, and platelet rich plasma (PRP) for PA assays. TT was prolonged by Hep device. The anticoagulant effect was proportional to Hep concentration (25 - >120 sec; 0.2 to 0.4 g/L). F content was unchanged compared to PPP (316±16.5 vs 322±35.7 mg/dL). The 0.1 M NaSa slice inhibited PA (42% IPA). TT time was enlarged (34.8±9.9 sec.), F content decreased (15% vs PPP) and IAP was higher (90% IAP, p<0.05) using 0.5 M NaSa. Both drugs doping showed an anticoagulant additive effect (TT: 60±20.5 sec.) with significant F diminution (49% vs PPP). Scanning electron microscopy showed that slices that contained 0.5 M NaSa or NaSa plus Hep, exhibited a complex microtubular structure. The anticoagulant effect of NaSa slices could be due to the hiding of F in these three-dimensional structures. Platelet deposition was rule out since platelet counting in the remaining PRP was not changed respect to basal count. The results suggest that, these devices are able to produce an in situ beneficial regulation of hemostasis, and could represent a potential design to prevent restenosis of blood vessels.Fil: Valle, María Ivone. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Flamini, Daniel Omar. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Sandoval, Marisa Julia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Saidman, Silvana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; ArgentinaFil: Massheimer, Virginia Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaLXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual Sociedad Argentina de Inmunología y XLVIII Reunión Anual Sociedad Argentina de Farmacología ExperimentalMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de Farmacología ExperimentalFundación Revista Medicina2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/235551Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual Sociedad Argentina de Inmunología y XLVIII Reunión Anual Sociedad Argentina de Farmacología Experimental; Mar del Plata; Argentina; 2016; 1-70025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:39:53Zoai:ri.conicet.gov.ar:11336/235551instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:39:53.674CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs
title Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs
spellingShingle Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs
Valle, María Ivone
HEMOSTASIS
NITINOL
HEPARIN
ANTICOAGULANT ACTIVITY
title_short Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs
title_full Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs
title_fullStr Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs
title_full_unstemmed Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs
title_sort Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs
dc.creator.none.fl_str_mv Valle, María Ivone
Flamini, Daniel Omar
Sandoval, Marisa Julia
Saidman, Silvana Beatriz
Massheimer, Virginia Laura
author Valle, María Ivone
author_facet Valle, María Ivone
Flamini, Daniel Omar
Sandoval, Marisa Julia
Saidman, Silvana Beatriz
Massheimer, Virginia Laura
author_role author
author2 Flamini, Daniel Omar
Sandoval, Marisa Julia
Saidman, Silvana Beatriz
Massheimer, Virginia Laura
author2_role author
author
author
author
dc.subject.none.fl_str_mv HEMOSTASIS
NITINOL
HEPARIN
ANTICOAGULANT ACTIVITY
topic HEMOSTASIS
NITINOL
HEPARIN
ANTICOAGULANT ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hemostasis plays an active role in atherosclerotic disease and arterial stenosis. Nitinol (NiTi) is used in several biomedical applications such as stents. Despite its biocompatibility, elasticity and corrosion resistance, some Ni2+ and Ti2+ ions may be released, resulting in local and systemic unwanted effects. The use of polypyrrole (PPy) coating, protects from ion release and, allows doping with drugs designed such as heparin (Hep) and sodium salicylate (NaSa). This work evaluates the effect on blood coagulation and platelet aggregation (PA) of NiTi-PPy devices (slices of 1cm2) doped with Hep (0.2; 0.3; 0.4 g/L), NaSa (0.1 and 0.5 M) or the combination of NaSa plus Hep (0.5M + 0.2 g/L). The slices were incubated 35 min with human plasma, and immediately after aliquots were taken for coagulation tests. Platelet poor plasma (PPP) was used for thrombin time (TT) and fibrinogen (F) measurements, and platelet rich plasma (PRP) for PA assays. TT was prolonged by Hep device. The anticoagulant effect was proportional to Hep concentration (25 - >120 sec; 0.2 to 0.4 g/L). F content was unchanged compared to PPP (316±16.5 vs 322±35.7 mg/dL). The 0.1 M NaSa slice inhibited PA (42% IPA). TT time was enlarged (34.8±9.9 sec.), F content decreased (15% vs PPP) and IAP was higher (90% IAP, p<0.05) using 0.5 M NaSa. Both drugs doping showed an anticoagulant additive effect (TT: 60±20.5 sec.) with significant F diminution (49% vs PPP). Scanning electron microscopy showed that slices that contained 0.5 M NaSa or NaSa plus Hep, exhibited a complex microtubular structure. The anticoagulant effect of NaSa slices could be due to the hiding of F in these three-dimensional structures. Platelet deposition was rule out since platelet counting in the remaining PRP was not changed respect to basal count. The results suggest that, these devices are able to produce an in situ beneficial regulation of hemostasis, and could represent a potential design to prevent restenosis of blood vessels.
Fil: Valle, María Ivone. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Flamini, Daniel Omar. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Sandoval, Marisa Julia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Saidman, Silvana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; Argentina
Fil: Massheimer, Virginia Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual Sociedad Argentina de Inmunología y XLVIII Reunión Anual Sociedad Argentina de Farmacología Experimental
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Farmacología Experimental
description Hemostasis plays an active role in atherosclerotic disease and arterial stenosis. Nitinol (NiTi) is used in several biomedical applications such as stents. Despite its biocompatibility, elasticity and corrosion resistance, some Ni2+ and Ti2+ ions may be released, resulting in local and systemic unwanted effects. The use of polypyrrole (PPy) coating, protects from ion release and, allows doping with drugs designed such as heparin (Hep) and sodium salicylate (NaSa). This work evaluates the effect on blood coagulation and platelet aggregation (PA) of NiTi-PPy devices (slices of 1cm2) doped with Hep (0.2; 0.3; 0.4 g/L), NaSa (0.1 and 0.5 M) or the combination of NaSa plus Hep (0.5M + 0.2 g/L). The slices were incubated 35 min with human plasma, and immediately after aliquots were taken for coagulation tests. Platelet poor plasma (PPP) was used for thrombin time (TT) and fibrinogen (F) measurements, and platelet rich plasma (PRP) for PA assays. TT was prolonged by Hep device. The anticoagulant effect was proportional to Hep concentration (25 - >120 sec; 0.2 to 0.4 g/L). F content was unchanged compared to PPP (316±16.5 vs 322±35.7 mg/dL). The 0.1 M NaSa slice inhibited PA (42% IPA). TT time was enlarged (34.8±9.9 sec.), F content decreased (15% vs PPP) and IAP was higher (90% IAP, p<0.05) using 0.5 M NaSa. Both drugs doping showed an anticoagulant additive effect (TT: 60±20.5 sec.) with significant F diminution (49% vs PPP). Scanning electron microscopy showed that slices that contained 0.5 M NaSa or NaSa plus Hep, exhibited a complex microtubular structure. The anticoagulant effect of NaSa slices could be due to the hiding of F in these three-dimensional structures. Platelet deposition was rule out since platelet counting in the remaining PRP was not changed respect to basal count. The results suggest that, these devices are able to produce an in situ beneficial regulation of hemostasis, and could represent a potential design to prevent restenosis of blood vessels.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/235551
Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual Sociedad Argentina de Inmunología y XLVIII Reunión Anual Sociedad Argentina de Farmacología Experimental; Mar del Plata; Argentina; 2016; 1-7
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/235551
identifier_str_mv Analysis of anticoagulant and antiplatelet capacity of Nitinol-Polypyrrole devices doped with drugs; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual Sociedad Argentina de Inmunología y XLVIII Reunión Anual Sociedad Argentina de Farmacología Experimental; Mar del Plata; Argentina; 2016; 1-7
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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