Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids

Autores
Zambrano Siri, Romina Trinidad; Beati, Maria Paula; Inchausti, Lucas; Smircich, Pablo; Alonso, Guillermo Daniel; Ocampo, Josefina
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi, Trypanosoma brucei and Leishmania major, usually known as TriTryps, are the causal agents of animal and human sickness, and are characterized by having complex life cycles, alternating between a mammalian host and an insect vector. Their genes are organized in long transcriptional units that give rise to polycistronic transcripts which maturate into mRNA by a process known as trans-splicing. Among those genes, an important subset is composed of multi-copy genes, which play crucial roles in host invasion and immune evasion. Here, we predicted the most likely trans-splicing acceptor sites (TASs) for TriTryps and found that the average chromatin organization is very similar among them with a mild nucleosome depletion at the TASs, and the same layout is observed in most of the genome. A detailed examination of the nucleosome landscapes resulting from different levels of chromatin digestion in T. brucei shows that an MNase-sensitive complex is protecting the TASs, and it is at least partly composed of histones. Additionally, comparative analysis for single and multi-copy genes in T. cruzi revealed a differential chromatin structure at the TASs suggesting a novel mechanism to guarantee the fidelity of trans-splicing in trypanosomatids.
Fil: Zambrano Siri, Romina Trinidad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Beati, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Inchausti, Lucas. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Smircich, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ocampo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
NUCLEOSOMES
TRYPANOSOMA CRUZI
CHROMATIN
HISTONES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/287996
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/287996
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Beyond the transcript: Chromatin implications in trans-splicing in TrypanosomatidsZambrano Siri, Romina TrinidadBeati, Maria PaulaInchausti, LucasSmircich, PabloAlonso, Guillermo DanielOcampo, JosefinaNUCLEOSOMESTRYPANOSOMA CRUZICHROMATINHISTONEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi, Trypanosoma brucei and Leishmania major, usually known as TriTryps, are the causal agents of animal and human sickness, and are characterized by having complex life cycles, alternating between a mammalian host and an insect vector. Their genes are organized in long transcriptional units that give rise to polycistronic transcripts which maturate into mRNA by a process known as trans-splicing. Among those genes, an important subset is composed of multi-copy genes, which play crucial roles in host invasion and immune evasion. Here, we predicted the most likely trans-splicing acceptor sites (TASs) for TriTryps and found that the average chromatin organization is very similar among them with a mild nucleosome depletion at the TASs, and the same layout is observed in most of the genome. A detailed examination of the nucleosome landscapes resulting from different levels of chromatin digestion in T. brucei shows that an MNase-sensitive complex is protecting the TASs, and it is at least partly composed of histones. Additionally, comparative analysis for single and multi-copy genes in T. cruzi revealed a differential chromatin structure at the TASs suggesting a novel mechanism to guarantee the fidelity of trans-splicing in trypanosomatids.Fil: Zambrano Siri, Romina Trinidad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Beati, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Inchausti, Lucas. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Smircich, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ocampo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaPublic Library of Science2026-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/287996Zambrano Siri, Romina Trinidad; Beati, Maria Paula; Inchausti, Lucas; Smircich, Pablo; Alonso, Guillermo Daniel; et al.; Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids; Public Library of Science; Plos One; 21; 2; 2-2026; 1-191932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0343367info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0343367info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-17T09:40:09Zoai:ri.conicet.gov.ar:11336/287996instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-17 09:40:10.103CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids
title Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids
spellingShingle Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids
Zambrano Siri, Romina Trinidad
NUCLEOSOMES
TRYPANOSOMA CRUZI
CHROMATIN
HISTONES
title_short Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids
title_full Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids
title_fullStr Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids
title_full_unstemmed Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids
title_sort Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids
dc.creator.none.fl_str_mv Zambrano Siri, Romina Trinidad
Beati, Maria Paula
Inchausti, Lucas
Smircich, Pablo
Alonso, Guillermo Daniel
Ocampo, Josefina
author Zambrano Siri, Romina Trinidad
author_facet Zambrano Siri, Romina Trinidad
Beati, Maria Paula
Inchausti, Lucas
Smircich, Pablo
Alonso, Guillermo Daniel
Ocampo, Josefina
author_role author
author2 Beati, Maria Paula
Inchausti, Lucas
Smircich, Pablo
Alonso, Guillermo Daniel
Ocampo, Josefina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv NUCLEOSOMES
TRYPANOSOMA CRUZI
CHROMATIN
HISTONES
topic NUCLEOSOMES
TRYPANOSOMA CRUZI
CHROMATIN
HISTONES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi, Trypanosoma brucei and Leishmania major, usually known as TriTryps, are the causal agents of animal and human sickness, and are characterized by having complex life cycles, alternating between a mammalian host and an insect vector. Their genes are organized in long transcriptional units that give rise to polycistronic transcripts which maturate into mRNA by a process known as trans-splicing. Among those genes, an important subset is composed of multi-copy genes, which play crucial roles in host invasion and immune evasion. Here, we predicted the most likely trans-splicing acceptor sites (TASs) for TriTryps and found that the average chromatin organization is very similar among them with a mild nucleosome depletion at the TASs, and the same layout is observed in most of the genome. A detailed examination of the nucleosome landscapes resulting from different levels of chromatin digestion in T. brucei shows that an MNase-sensitive complex is protecting the TASs, and it is at least partly composed of histones. Additionally, comparative analysis for single and multi-copy genes in T. cruzi revealed a differential chromatin structure at the TASs suggesting a novel mechanism to guarantee the fidelity of trans-splicing in trypanosomatids.
Fil: Zambrano Siri, Romina Trinidad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Beati, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Inchausti, Lucas. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Smircich, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ocampo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description Trypanosoma cruzi, Trypanosoma brucei and Leishmania major, usually known as TriTryps, are the causal agents of animal and human sickness, and are characterized by having complex life cycles, alternating between a mammalian host and an insect vector. Their genes are organized in long transcriptional units that give rise to polycistronic transcripts which maturate into mRNA by a process known as trans-splicing. Among those genes, an important subset is composed of multi-copy genes, which play crucial roles in host invasion and immune evasion. Here, we predicted the most likely trans-splicing acceptor sites (TASs) for TriTryps and found that the average chromatin organization is very similar among them with a mild nucleosome depletion at the TASs, and the same layout is observed in most of the genome. A detailed examination of the nucleosome landscapes resulting from different levels of chromatin digestion in T. brucei shows that an MNase-sensitive complex is protecting the TASs, and it is at least partly composed of histones. Additionally, comparative analysis for single and multi-copy genes in T. cruzi revealed a differential chromatin structure at the TASs suggesting a novel mechanism to guarantee the fidelity of trans-splicing in trypanosomatids.
publishDate 2026
dc.date.none.fl_str_mv 2026-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/287996
Zambrano Siri, Romina Trinidad; Beati, Maria Paula; Inchausti, Lucas; Smircich, Pablo; Alonso, Guillermo Daniel; et al.; Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids; Public Library of Science; Plos One; 21; 2; 2-2026; 1-19
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/287996
identifier_str_mv Zambrano Siri, Romina Trinidad; Beati, Maria Paula; Inchausti, Lucas; Smircich, Pablo; Alonso, Guillermo Daniel; et al.; Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids; Public Library of Science; Plos One; 21; 2; 2-2026; 1-19
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0343367
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0343367
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.040872

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