Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy

Autores
Smaldini, Paola Lorena; Ibañez, Andres Esteban; Fossati, Carlos Alberto; Cassataro, Juliana; Docena, Guillermo H.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Food allergies are increasingly common disorders and no therapeutic strategies are yet approved. The unlipidated Omp16 (U-Omp16) is the outer membrane protein of 16 kDa from B. abortus and possesses a mucosal adjuvant property. In this study, we aimed to examine the U-Omp16 capacity to abrogate an allergen-specific Th2 immune response when it is administered as an oral adjuvant in a mouse model of food allergy.Balb/c mice were sensitized with cholera toxin and cow’s milk proteins (cMP) by gavage and simultaneously treated with U-Omp16 and cMP. Oral challenge with cMP was performed to evaluate the allergic status of mice. symptoms, local (small bowel cytokine and transcription factor gene expression) and systemic (specific isotypes and spleen cell-secreted cytokines) parameters, and skin tests were done to evaluate the immune response.We found that the oral administration of U-Omp16 with cMP during sensitization dampened the allergic symptoms, with negativization of immediate skin test and increased skin DTh response. serum specific Ige and IL-5 were inhibited and a Th1 response was promoted (specific IgG2a antibodies and cMP-induced IFN-γ secretion). We found at the muco-sal site an inhibition of the gene expression corresponding to IL-13 and Gata-3, with an induction of IFN-γ and T-bet.These results indicated that the oral administration of U-Omp16 significantly controlled the allergic response in sen-sitized mice with a shift of the balance of Th1- and Th2-T cells toward Th1 predominance. These findings suggest that U-Omp16 may be useful as a Th1-directing adjuvant in an oral vaccine.
Fil: Smaldini, Paola Lorena. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Ibañez, Andres Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Fossati, Carlos Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Cassataro, Juliana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Invest. Biotec. (subsede San Martin) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Invest. Biotec. (subsede San Martin); Argentina
Fil: Docena, Guillermo H.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
Milk Allergy
U-Omp16
Oral Adjuvant
Ige
Immunomodulation
Intestinal Mucosa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32311
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32311
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergySmaldini, Paola LorenaIbañez, Andres EstebanFossati, Carlos AlbertoCassataro, JulianaDocena, Guillermo H.Milk AllergyU-Omp16Oral AdjuvantIgeImmunomodulationIntestinal Mucosahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Food allergies are increasingly common disorders and no therapeutic strategies are yet approved. The unlipidated Omp16 (U-Omp16) is the outer membrane protein of 16 kDa from B. abortus and possesses a mucosal adjuvant property. In this study, we aimed to examine the U-Omp16 capacity to abrogate an allergen-specific Th2 immune response when it is administered as an oral adjuvant in a mouse model of food allergy.Balb/c mice were sensitized with cholera toxin and cow’s milk proteins (cMP) by gavage and simultaneously treated with U-Omp16 and cMP. Oral challenge with cMP was performed to evaluate the allergic status of mice. symptoms, local (small bowel cytokine and transcription factor gene expression) and systemic (specific isotypes and spleen cell-secreted cytokines) parameters, and skin tests were done to evaluate the immune response.We found that the oral administration of U-Omp16 with cMP during sensitization dampened the allergic symptoms, with negativization of immediate skin test and increased skin DTh response. serum specific Ige and IL-5 were inhibited and a Th1 response was promoted (specific IgG2a antibodies and cMP-induced IFN-γ secretion). We found at the muco-sal site an inhibition of the gene expression corresponding to IL-13 and Gata-3, with an induction of IFN-γ and T-bet.These results indicated that the oral administration of U-Omp16 significantly controlled the allergic response in sen-sitized mice with a shift of the balance of Th1- and Th2-T cells toward Th1 predominance. These findings suggest that U-Omp16 may be useful as a Th1-directing adjuvant in an oral vaccine.Fil: Smaldini, Paola Lorena. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Ibañez, Andres Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Fossati, Carlos Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Cassataro, Juliana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Invest. Biotec. (subsede San Martin) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Invest. Biotec. (subsede San Martin); ArgentinaFil: Docena, Guillermo H.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaLandes Bioscience2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32311Smaldini, Paola Lorena; Ibañez, Andres Esteban; Fossati, Carlos Alberto; Cassataro, Juliana; Docena, Guillermo H.; Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy; Landes Bioscience; Human vaccines & immunotherapeutics; 10; 7; 5-2014; 1-62164-554XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4161/hv.28845info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.4161/hv.28845info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:27Zoai:ri.conicet.gov.ar:11336/32311instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:27.648CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy
title Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy
spellingShingle Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy
Smaldini, Paola Lorena
Milk Allergy
U-Omp16
Oral Adjuvant
Ige
Immunomodulation
Intestinal Mucosa
title_short Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy
title_full Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy
title_fullStr Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy
title_full_unstemmed Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy
title_sort Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy
dc.creator.none.fl_str_mv Smaldini, Paola Lorena
Ibañez, Andres Esteban
Fossati, Carlos Alberto
Cassataro, Juliana
Docena, Guillermo H.
author Smaldini, Paola Lorena
author_facet Smaldini, Paola Lorena
Ibañez, Andres Esteban
Fossati, Carlos Alberto
Cassataro, Juliana
Docena, Guillermo H.
author_role author
author2 Ibañez, Andres Esteban
Fossati, Carlos Alberto
Cassataro, Juliana
Docena, Guillermo H.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Milk Allergy
U-Omp16
Oral Adjuvant
Ige
Immunomodulation
Intestinal Mucosa
topic Milk Allergy
U-Omp16
Oral Adjuvant
Ige
Immunomodulation
Intestinal Mucosa
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Food allergies are increasingly common disorders and no therapeutic strategies are yet approved. The unlipidated Omp16 (U-Omp16) is the outer membrane protein of 16 kDa from B. abortus and possesses a mucosal adjuvant property. In this study, we aimed to examine the U-Omp16 capacity to abrogate an allergen-specific Th2 immune response when it is administered as an oral adjuvant in a mouse model of food allergy.Balb/c mice were sensitized with cholera toxin and cow’s milk proteins (cMP) by gavage and simultaneously treated with U-Omp16 and cMP. Oral challenge with cMP was performed to evaluate the allergic status of mice. symptoms, local (small bowel cytokine and transcription factor gene expression) and systemic (specific isotypes and spleen cell-secreted cytokines) parameters, and skin tests were done to evaluate the immune response.We found that the oral administration of U-Omp16 with cMP during sensitization dampened the allergic symptoms, with negativization of immediate skin test and increased skin DTh response. serum specific Ige and IL-5 were inhibited and a Th1 response was promoted (specific IgG2a antibodies and cMP-induced IFN-γ secretion). We found at the muco-sal site an inhibition of the gene expression corresponding to IL-13 and Gata-3, with an induction of IFN-γ and T-bet.These results indicated that the oral administration of U-Omp16 significantly controlled the allergic response in sen-sitized mice with a shift of the balance of Th1- and Th2-T cells toward Th1 predominance. These findings suggest that U-Omp16 may be useful as a Th1-directing adjuvant in an oral vaccine.
Fil: Smaldini, Paola Lorena. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Ibañez, Andres Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Fossati, Carlos Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Cassataro, Juliana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Invest. Biotec. (subsede San Martin) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Invest. Biotec. (subsede San Martin); Argentina
Fil: Docena, Guillermo H.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Food allergies are increasingly common disorders and no therapeutic strategies are yet approved. The unlipidated Omp16 (U-Omp16) is the outer membrane protein of 16 kDa from B. abortus and possesses a mucosal adjuvant property. In this study, we aimed to examine the U-Omp16 capacity to abrogate an allergen-specific Th2 immune response when it is administered as an oral adjuvant in a mouse model of food allergy.Balb/c mice were sensitized with cholera toxin and cow’s milk proteins (cMP) by gavage and simultaneously treated with U-Omp16 and cMP. Oral challenge with cMP was performed to evaluate the allergic status of mice. symptoms, local (small bowel cytokine and transcription factor gene expression) and systemic (specific isotypes and spleen cell-secreted cytokines) parameters, and skin tests were done to evaluate the immune response.We found that the oral administration of U-Omp16 with cMP during sensitization dampened the allergic symptoms, with negativization of immediate skin test and increased skin DTh response. serum specific Ige and IL-5 were inhibited and a Th1 response was promoted (specific IgG2a antibodies and cMP-induced IFN-γ secretion). We found at the muco-sal site an inhibition of the gene expression corresponding to IL-13 and Gata-3, with an induction of IFN-γ and T-bet.These results indicated that the oral administration of U-Omp16 significantly controlled the allergic response in sen-sitized mice with a shift of the balance of Th1- and Th2-T cells toward Th1 predominance. These findings suggest that U-Omp16 may be useful as a Th1-directing adjuvant in an oral vaccine.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32311
Smaldini, Paola Lorena; Ibañez, Andres Esteban; Fossati, Carlos Alberto; Cassataro, Juliana; Docena, Guillermo H.; Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy; Landes Bioscience; Human vaccines & immunotherapeutics; 10; 7; 5-2014; 1-6
2164-554X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32311
identifier_str_mv Smaldini, Paola Lorena; Ibañez, Andres Esteban; Fossati, Carlos Alberto; Cassataro, Juliana; Docena, Guillermo H.; Oral delivery of Brucella spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy; Landes Bioscience; Human vaccines & immunotherapeutics; 10; 7; 5-2014; 1-6
2164-554X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4161/hv.28845
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.4161/hv.28845
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Landes Bioscience
publisher.none.fl_str_mv Landes Bioscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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