Addition of inorganic compounds to increase laccase enzyme activity

Autores
Molina, Melina Antonella; Sgroppo, Sonia Cecilia; Milde, Laura Beatríz; Zapata, Pedro Dario; Fonseca, Maria Isabel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction: Fungal laccases have shown great potential in industrial and environmentalapplications. Therefore, further study of the properties of the laccase enzymeand its behavior with the addition of different compounds is essential to addressthe potential applications of this enzyme to specific fields.Objective:Evaluate the ability of different inorganic compounds to increase the activity offungal laccases.Materials and methods:To study the effects of different compounds on the activity of laccaserecombianant, we work with a laccase from Phlebia brevispora heterologouslyexpressed.The effects of Iron chloride, Copper sulphate, Calcium carbonate, Zinc chloride,Potassium chloride and Ammonium sulfate on the activity of laccase weredetermined.Solution of 0.5mmol L-1 of each compound were tested and the residualenzymatic activity were measured and compared with control (withoutcompound). Later, the solution which causes the highest laccase activity, wasused in concentrations from 0,5 mmol L-1to 3 mmoL-1to quantify the residualactivity.Results:In the production of recombinant laccases assayed on flask, Iron sulfate 1 mmolL-1 showed the highest enzymatic activity with an activity peak of 613.00 EU(μmoL min-1 mL-1) at the fourth day of incubation. The other compoundsassayed showed the following activity peaks: Iron chloride 373.02 UL-1, Calciumcarbonate 256.3 UL -1, Zinc chloride 47.10 UL-1, Potassium chloride 87.09 UL-1,Ammonium sulfate 101.03 UL-1.Conclusion:Based on the results of this work, we conclude that the laccase enzymesupplemented with Iron sulfate offers promising results for the application ofwhite-rot fungal resources in environmental remediation, bakery industry,bioremediation, biodegradation and so forth.
Fil: Molina, Melina Antonella. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Sgroppo, Sonia Cecilia. Universidad Nacional del Nordeste; Argentina
Fil: Milde, Laura Beatríz. Universidad Nacional de Misiones; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
I Simpósio de Microbiologia de Rondônia: Saúde, Ambiente e Inovação
Brasil
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Materia
LACCASE
INORGANIC COMPOUNDS
induction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201542
Acceder
id CONICETDig_8cbc4b59ff0fc245fbaa0108c2e0ed65
oai_identifier_str oai:ri.conicet.gov.ar:11336/201542
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Addition of inorganic compounds to increase laccase enzyme activityMolina, Melina AntonellaSgroppo, Sonia CeciliaMilde, Laura BeatrízZapata, Pedro DarioFonseca, Maria IsabelLACCASEINORGANIC COMPOUNDSinductionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: Fungal laccases have shown great potential in industrial and environmentalapplications. Therefore, further study of the properties of the laccase enzymeand its behavior with the addition of different compounds is essential to addressthe potential applications of this enzyme to specific fields.Objective:Evaluate the ability of different inorganic compounds to increase the activity offungal laccases.Materials and methods:To study the effects of different compounds on the activity of laccaserecombianant, we work with a laccase from Phlebia brevispora heterologouslyexpressed.The effects of Iron chloride, Copper sulphate, Calcium carbonate, Zinc chloride,Potassium chloride and Ammonium sulfate on the activity of laccase weredetermined.Solution of 0.5mmol L-1 of each compound were tested and the residualenzymatic activity were measured and compared with control (withoutcompound). Later, the solution which causes the highest laccase activity, wasused in concentrations from 0,5 mmol L-1to 3 mmoL-1to quantify the residualactivity.Results:In the production of recombinant laccases assayed on flask, Iron sulfate 1 mmolL-1 showed the highest enzymatic activity with an activity peak of 613.00 EU(μmoL min-1 mL-1) at the fourth day of incubation. The other compoundsassayed showed the following activity peaks: Iron chloride 373.02 UL-1, Calciumcarbonate 256.3 UL -1, Zinc chloride 47.10 UL-1, Potassium chloride 87.09 UL-1,Ammonium sulfate 101.03 UL-1.Conclusion:Based on the results of this work, we conclude that the laccase enzymesupplemented with Iron sulfate offers promising results for the application ofwhite-rot fungal resources in environmental remediation, bakery industry,bioremediation, biodegradation and so forth.Fil: Molina, Melina Antonella. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Sgroppo, Sonia Cecilia. Universidad Nacional del Nordeste; ArgentinaFil: Milde, Laura Beatríz. Universidad Nacional de Misiones; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaI Simpósio de Microbiologia de Rondônia: Saúde, Ambiente e InovaçãoBrasilCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorCoordenação de Aperfeiçoamento de Pessoal de Nível Superior2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201542Addition of inorganic compounds to increase laccase enzyme activity; I Simpósio de Microbiologia de Rondônia: Saúde, Ambiente e Inovação; Brasil; 2021; 1-1978-65-86861-91-4CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://eventos.congresse.me/simicron/resumos/8813.pdf?version=originalInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:16:17Zoai:ri.conicet.gov.ar:11336/201542instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:16:17.825CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Addition of inorganic compounds to increase laccase enzyme activity
title Addition of inorganic compounds to increase laccase enzyme activity
spellingShingle Addition of inorganic compounds to increase laccase enzyme activity
Molina, Melina Antonella
LACCASE
INORGANIC COMPOUNDS
induction
title_short Addition of inorganic compounds to increase laccase enzyme activity
title_full Addition of inorganic compounds to increase laccase enzyme activity
title_fullStr Addition of inorganic compounds to increase laccase enzyme activity
title_full_unstemmed Addition of inorganic compounds to increase laccase enzyme activity
title_sort Addition of inorganic compounds to increase laccase enzyme activity
dc.creator.none.fl_str_mv Molina, Melina Antonella
Sgroppo, Sonia Cecilia
Milde, Laura Beatríz
Zapata, Pedro Dario
Fonseca, Maria Isabel
author Molina, Melina Antonella
author_facet Molina, Melina Antonella
Sgroppo, Sonia Cecilia
Milde, Laura Beatríz
Zapata, Pedro Dario
Fonseca, Maria Isabel
author_role author
author2 Sgroppo, Sonia Cecilia
Milde, Laura Beatríz
Zapata, Pedro Dario
Fonseca, Maria Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv LACCASE
INORGANIC COMPOUNDS
induction
topic LACCASE
INORGANIC COMPOUNDS
induction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: Fungal laccases have shown great potential in industrial and environmentalapplications. Therefore, further study of the properties of the laccase enzymeand its behavior with the addition of different compounds is essential to addressthe potential applications of this enzyme to specific fields.Objective:Evaluate the ability of different inorganic compounds to increase the activity offungal laccases.Materials and methods:To study the effects of different compounds on the activity of laccaserecombianant, we work with a laccase from Phlebia brevispora heterologouslyexpressed.The effects of Iron chloride, Copper sulphate, Calcium carbonate, Zinc chloride,Potassium chloride and Ammonium sulfate on the activity of laccase weredetermined.Solution of 0.5mmol L-1 of each compound were tested and the residualenzymatic activity were measured and compared with control (withoutcompound). Later, the solution which causes the highest laccase activity, wasused in concentrations from 0,5 mmol L-1to 3 mmoL-1to quantify the residualactivity.Results:In the production of recombinant laccases assayed on flask, Iron sulfate 1 mmolL-1 showed the highest enzymatic activity with an activity peak of 613.00 EU(μmoL min-1 mL-1) at the fourth day of incubation. The other compoundsassayed showed the following activity peaks: Iron chloride 373.02 UL-1, Calciumcarbonate 256.3 UL -1, Zinc chloride 47.10 UL-1, Potassium chloride 87.09 UL-1,Ammonium sulfate 101.03 UL-1.Conclusion:Based on the results of this work, we conclude that the laccase enzymesupplemented with Iron sulfate offers promising results for the application ofwhite-rot fungal resources in environmental remediation, bakery industry,bioremediation, biodegradation and so forth.
Fil: Molina, Melina Antonella. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Sgroppo, Sonia Cecilia. Universidad Nacional del Nordeste; Argentina
Fil: Milde, Laura Beatríz. Universidad Nacional de Misiones; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
I Simpósio de Microbiologia de Rondônia: Saúde, Ambiente e Inovação
Brasil
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
description Introduction: Fungal laccases have shown great potential in industrial and environmentalapplications. Therefore, further study of the properties of the laccase enzymeand its behavior with the addition of different compounds is essential to addressthe potential applications of this enzyme to specific fields.Objective:Evaluate the ability of different inorganic compounds to increase the activity offungal laccases.Materials and methods:To study the effects of different compounds on the activity of laccaserecombianant, we work with a laccase from Phlebia brevispora heterologouslyexpressed.The effects of Iron chloride, Copper sulphate, Calcium carbonate, Zinc chloride,Potassium chloride and Ammonium sulfate on the activity of laccase weredetermined.Solution of 0.5mmol L-1 of each compound were tested and the residualenzymatic activity were measured and compared with control (withoutcompound). Later, the solution which causes the highest laccase activity, wasused in concentrations from 0,5 mmol L-1to 3 mmoL-1to quantify the residualactivity.Results:In the production of recombinant laccases assayed on flask, Iron sulfate 1 mmolL-1 showed the highest enzymatic activity with an activity peak of 613.00 EU(μmoL min-1 mL-1) at the fourth day of incubation. The other compoundsassayed showed the following activity peaks: Iron chloride 373.02 UL-1, Calciumcarbonate 256.3 UL -1, Zinc chloride 47.10 UL-1, Potassium chloride 87.09 UL-1,Ammonium sulfate 101.03 UL-1.Conclusion:Based on the results of this work, we conclude that the laccase enzymesupplemented with Iron sulfate offers promising results for the application ofwhite-rot fungal resources in environmental remediation, bakery industry,bioremediation, biodegradation and so forth.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Simposio
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201542
Addition of inorganic compounds to increase laccase enzyme activity; I Simpósio de Microbiologia de Rondônia: Saúde, Ambiente e Inovação; Brasil; 2021; 1-1
978-65-86861-91-4
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201542
identifier_str_mv Addition of inorganic compounds to increase laccase enzyme activity; I Simpósio de Microbiologia de Rondônia: Saúde, Ambiente e Inovação; Brasil; 2021; 1-1
978-65-86861-91-4
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://eventos.congresse.me/simicron/resumos/8813.pdf?version=original
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
publisher.none.fl_str_mv Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980885746417664
score 13.004268

Publicaciones similares