A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina

Autores
Siano, Alvaro Sebastían; Gatti, Paula Ines; Imaz, María Susana; Zerbini, Elsa Virginia; Simonetta, Arturo Carlos; Lajmanovich, Rafael Carlos; Tonarelli, Georgina Guadalupe
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The potential of the skin of anuran amphibians as a new source of bioactive peptides was investigated . For this purpose, the study collected data in the mid-eastern region of Argentina. Two anuran amphibian species were studied which belong to the Hylidae and Leptodactylidae families, Leptodatylus latrans (Ll) and Hypsiboas pulchellus (Hp). Two methods for the extraction of bioactive components were compared in their effectiveness: solvent extraction (SE) and transcutaneous amphibian stimulation (TAS). Two different approaches were used to study the extracts: i) the direct analysis of the complete samples by MALDI-TOF-MS, and ii) the characterization of bioactive fractions obtained by HPLC and analyzed by MS. The results show that not only the composition of the samples obtained by SE and TAS is different but also their antimicrobial activity. In this sense, only the extracts obtained from Ll and Hp by SE inhibited the growth of Mycobacterium tuberculosis H37Rv. The inhibitory activity of the extracts against the butyrylcholinesterase enzyme (BChE) was also investigated. Samples of Ll showed low percentages of inhibition whereas in Hp samples, the inhibition was moderate (40 -44%). The results suggest that the inhibitory activity of Hp is related to the presence of low molecular weight compounds.
Fil: Siano, Alvaro Sebastían. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Gatti, Paula Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica; Argentina
Fil: Imaz, María Susana. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Zerbini, Elsa Virginia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica; Argentina
Fil: Simonetta, Arturo Carlos. Universidad Nacional del Litoral; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral; Argentina
Fil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Materia
amphibians
antimicrobial activity
butyrylcholinesterase inhibition
granular glands
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92798
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92798
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from ArgentinaSiano, Alvaro SebastíanGatti, Paula InesImaz, María SusanaZerbini, Elsa VirginiaSimonetta, Arturo CarlosLajmanovich, Rafael CarlosTonarelli, Georgina Guadalupeamphibiansantimicrobial activitybutyrylcholinesterase inhibitiongranular glandshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The potential of the skin of anuran amphibians as a new source of bioactive peptides was investigated . For this purpose, the study collected data in the mid-eastern region of Argentina. Two anuran amphibian species were studied which belong to the Hylidae and Leptodactylidae families, Leptodatylus latrans (Ll) and Hypsiboas pulchellus (Hp). Two methods for the extraction of bioactive components were compared in their effectiveness: solvent extraction (SE) and transcutaneous amphibian stimulation (TAS). Two different approaches were used to study the extracts: i) the direct analysis of the complete samples by MALDI-TOF-MS, and ii) the characterization of bioactive fractions obtained by HPLC and analyzed by MS. The results show that not only the composition of the samples obtained by SE and TAS is different but also their antimicrobial activity. In this sense, only the extracts obtained from Ll and Hp by SE inhibited the growth of Mycobacterium tuberculosis H37Rv. The inhibitory activity of the extracts against the butyrylcholinesterase enzyme (BChE) was also investigated. Samples of Ll showed low percentages of inhibition whereas in Hp samples, the inhibition was moderate (40 -44%). The results suggest that the inhibitory activity of Hp is related to the presence of low molecular weight compounds.Fil: Siano, Alvaro Sebastían. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; ArgentinaFil: Gatti, Paula Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica; ArgentinaFil: Imaz, María Susana. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; ArgentinaFil: Zerbini, Elsa Virginia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica; ArgentinaFil: Simonetta, Arturo Carlos. Universidad Nacional del Litoral; ArgentinaFil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral; ArgentinaFil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; ArgentinaACG Publications2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92798Siano, Alvaro Sebastían; Gatti, Paula Ines; Imaz, María Susana; Zerbini, Elsa Virginia; Simonetta, Arturo Carlos; et al.; A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina; ACG Publications; Records Of Natural Products; 8; 3-2014; 128-1351307-6167CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.acgpubs.org/RNP/2014/Volume8/Issue%201/18-RNP-1211-192.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:12Zoai:ri.conicet.gov.ar:11336/92798instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:12.421CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina
title A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina
spellingShingle A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina
Siano, Alvaro Sebastían
amphibians
antimicrobial activity
butyrylcholinesterase inhibition
granular glands
title_short A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina
title_full A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina
title_fullStr A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina
title_full_unstemmed A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina
title_sort A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina
dc.creator.none.fl_str_mv Siano, Alvaro Sebastían
Gatti, Paula Ines
Imaz, María Susana
Zerbini, Elsa Virginia
Simonetta, Arturo Carlos
Lajmanovich, Rafael Carlos
Tonarelli, Georgina Guadalupe
author Siano, Alvaro Sebastían
author_facet Siano, Alvaro Sebastían
Gatti, Paula Ines
Imaz, María Susana
Zerbini, Elsa Virginia
Simonetta, Arturo Carlos
Lajmanovich, Rafael Carlos
Tonarelli, Georgina Guadalupe
author_role author
author2 Gatti, Paula Ines
Imaz, María Susana
Zerbini, Elsa Virginia
Simonetta, Arturo Carlos
Lajmanovich, Rafael Carlos
Tonarelli, Georgina Guadalupe
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv amphibians
antimicrobial activity
butyrylcholinesterase inhibition
granular glands
topic amphibians
antimicrobial activity
butyrylcholinesterase inhibition
granular glands
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The potential of the skin of anuran amphibians as a new source of bioactive peptides was investigated . For this purpose, the study collected data in the mid-eastern region of Argentina. Two anuran amphibian species were studied which belong to the Hylidae and Leptodactylidae families, Leptodatylus latrans (Ll) and Hypsiboas pulchellus (Hp). Two methods for the extraction of bioactive components were compared in their effectiveness: solvent extraction (SE) and transcutaneous amphibian stimulation (TAS). Two different approaches were used to study the extracts: i) the direct analysis of the complete samples by MALDI-TOF-MS, and ii) the characterization of bioactive fractions obtained by HPLC and analyzed by MS. The results show that not only the composition of the samples obtained by SE and TAS is different but also their antimicrobial activity. In this sense, only the extracts obtained from Ll and Hp by SE inhibited the growth of Mycobacterium tuberculosis H37Rv. The inhibitory activity of the extracts against the butyrylcholinesterase enzyme (BChE) was also investigated. Samples of Ll showed low percentages of inhibition whereas in Hp samples, the inhibition was moderate (40 -44%). The results suggest that the inhibitory activity of Hp is related to the presence of low molecular weight compounds.
Fil: Siano, Alvaro Sebastían. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Gatti, Paula Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica; Argentina
Fil: Imaz, María Susana. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Zerbini, Elsa Virginia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica; Argentina
Fil: Simonetta, Arturo Carlos. Universidad Nacional del Litoral; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral; Argentina
Fil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
description The potential of the skin of anuran amphibians as a new source of bioactive peptides was investigated . For this purpose, the study collected data in the mid-eastern region of Argentina. Two anuran amphibian species were studied which belong to the Hylidae and Leptodactylidae families, Leptodatylus latrans (Ll) and Hypsiboas pulchellus (Hp). Two methods for the extraction of bioactive components were compared in their effectiveness: solvent extraction (SE) and transcutaneous amphibian stimulation (TAS). Two different approaches were used to study the extracts: i) the direct analysis of the complete samples by MALDI-TOF-MS, and ii) the characterization of bioactive fractions obtained by HPLC and analyzed by MS. The results show that not only the composition of the samples obtained by SE and TAS is different but also their antimicrobial activity. In this sense, only the extracts obtained from Ll and Hp by SE inhibited the growth of Mycobacterium tuberculosis H37Rv. The inhibitory activity of the extracts against the butyrylcholinesterase enzyme (BChE) was also investigated. Samples of Ll showed low percentages of inhibition whereas in Hp samples, the inhibition was moderate (40 -44%). The results suggest that the inhibitory activity of Hp is related to the presence of low molecular weight compounds.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92798
Siano, Alvaro Sebastían; Gatti, Paula Ines; Imaz, María Susana; Zerbini, Elsa Virginia; Simonetta, Arturo Carlos; et al.; A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina; ACG Publications; Records Of Natural Products; 8; 3-2014; 128-135
1307-6167
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92798
identifier_str_mv Siano, Alvaro Sebastían; Gatti, Paula Ines; Imaz, María Susana; Zerbini, Elsa Virginia; Simonetta, Arturo Carlos; et al.; A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina; ACG Publications; Records Of Natural Products; 8; 3-2014; 128-135
1307-6167
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.acgpubs.org/RNP/2014/Volume8/Issue%201/18-RNP-1211-192.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv ACG Publications
publisher.none.fl_str_mv ACG Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269993693085696
score 13.13397

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