Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia

Autores
Arena, Miriam Elisabet; Martínez Pastur, Guillermo José; Benavides, Maria Patricia; Zappacosta, Diego Carlos; Eliasco, Eleonora; Curvetto, Nestor Raul
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Berberis buxifolia is a Patagonian shrub with great economic potential for tinctorial, pharmacological, and food industries. Clonal propagation is possible through in vitro culture and is also useful for metabolite production. However, this species is difficult to root, and to improve this, more knowledge of rhizogenesis processes is needed. Polyamines and peroxidases are useful biochemical markers during analysis of rooting phases for correlation with tissue morphological changes. Therefore, endogenous polyamine (putrescine, spermidine, spermine) changes, peroxidase activity evolution, and morphological development were studied to characterise the in vitro rhizogenesis of microshoots of B. buxifolia and, thus, to define the rooting phases. Polyamine and peroxidase changed significantly during the rooting period, and had opposite behaviours which were directly related to the IBA media content. The lower polyamine concentration and the higher peroxidase activity were found in a treatment with a dark period during the first four days and with IBA in the culture medium. Putrescine was the most abundant polyamine found in B. buxifolia tissues, 14- to 18-fold more than spermidine and spermine, respectively. Therefore, these compounds were used to define the rooting phases: an induction phase (0 to 4–7 days) followed by an expression phase (4–7 to 28 days). The observed changes in the biochemical markers could be correlated with microscopic and macroscopic tissue observations in the microshoots, and the time course of rooting percentage. Successive culture media can be developed including polyamines, or other compounds and environmental conditions, which positively modify the studied biochemical markers behaviour.
Fil: Arena, Miriam Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Martínez Pastur, Guillermo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Eliasco, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
Rhizogenesis
Rooting Markers
Histology
Micropropagation
Patagonia
Berberis Buxifolia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33625
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/33625
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifoliaArena, Miriam ElisabetMartínez Pastur, Guillermo JoséBenavides, Maria PatriciaZappacosta, Diego CarlosEliasco, EleonoraCurvetto, Nestor RaulRhizogenesisRooting MarkersHistologyMicropropagationPatagoniaBerberis Buxifoliahttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Berberis buxifolia is a Patagonian shrub with great economic potential for tinctorial, pharmacological, and food industries. Clonal propagation is possible through in vitro culture and is also useful for metabolite production. However, this species is difficult to root, and to improve this, more knowledge of rhizogenesis processes is needed. Polyamines and peroxidases are useful biochemical markers during analysis of rooting phases for correlation with tissue morphological changes. Therefore, endogenous polyamine (putrescine, spermidine, spermine) changes, peroxidase activity evolution, and morphological development were studied to characterise the in vitro rhizogenesis of microshoots of B. buxifolia and, thus, to define the rooting phases. Polyamine and peroxidase changed significantly during the rooting period, and had opposite behaviours which were directly related to the IBA media content. The lower polyamine concentration and the higher peroxidase activity were found in a treatment with a dark period during the first four days and with IBA in the culture medium. Putrescine was the most abundant polyamine found in B. buxifolia tissues, 14- to 18-fold more than spermidine and spermine, respectively. Therefore, these compounds were used to define the rooting phases: an induction phase (0 to 4–7 days) followed by an expression phase (4–7 to 28 days). The observed changes in the biochemical markers could be correlated with microscopic and macroscopic tissue observations in the microshoots, and the time course of rooting percentage. Successive culture media can be developed including polyamines, or other compounds and environmental conditions, which positively modify the studied biochemical markers behaviour.Fil: Arena, Miriam Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Martínez Pastur, Guillermo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Eliasco, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaRsnz Publishing2003-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33625Arena, Miriam Elisabet; Curvetto, Nestor Raul; Zappacosta, Diego Carlos; Benavides, Maria Patricia; Martínez Pastur, Guillermo José; Eliasco, Eleonora; et al.; Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia; Rsnz Publishing; New Zealand Journal of Botany; 41; 3; 12-2003; 475-4850028-825XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/0028825X.2003.9512864info:eu-repo/semantics/altIdentifier/doi/10.1080/0028825X.2003.9512864info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:48Zoai:ri.conicet.gov.ar:11336/33625instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:48.348CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia
title Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia
spellingShingle Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia
Arena, Miriam Elisabet
Rhizogenesis
Rooting Markers
Histology
Micropropagation
Patagonia
Berberis Buxifolia
title_short Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia
title_full Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia
title_fullStr Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia
title_full_unstemmed Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia
title_sort Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia
dc.creator.none.fl_str_mv Arena, Miriam Elisabet
Martínez Pastur, Guillermo José
Benavides, Maria Patricia
Zappacosta, Diego Carlos
Eliasco, Eleonora
Curvetto, Nestor Raul
author Arena, Miriam Elisabet
author_facet Arena, Miriam Elisabet
Martínez Pastur, Guillermo José
Benavides, Maria Patricia
Zappacosta, Diego Carlos
Eliasco, Eleonora
Curvetto, Nestor Raul
author_role author
author2 Martínez Pastur, Guillermo José
Benavides, Maria Patricia
Zappacosta, Diego Carlos
Eliasco, Eleonora
Curvetto, Nestor Raul
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Rhizogenesis
Rooting Markers
Histology
Micropropagation
Patagonia
Berberis Buxifolia
topic Rhizogenesis
Rooting Markers
Histology
Micropropagation
Patagonia
Berberis Buxifolia
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Berberis buxifolia is a Patagonian shrub with great economic potential for tinctorial, pharmacological, and food industries. Clonal propagation is possible through in vitro culture and is also useful for metabolite production. However, this species is difficult to root, and to improve this, more knowledge of rhizogenesis processes is needed. Polyamines and peroxidases are useful biochemical markers during analysis of rooting phases for correlation with tissue morphological changes. Therefore, endogenous polyamine (putrescine, spermidine, spermine) changes, peroxidase activity evolution, and morphological development were studied to characterise the in vitro rhizogenesis of microshoots of B. buxifolia and, thus, to define the rooting phases. Polyamine and peroxidase changed significantly during the rooting period, and had opposite behaviours which were directly related to the IBA media content. The lower polyamine concentration and the higher peroxidase activity were found in a treatment with a dark period during the first four days and with IBA in the culture medium. Putrescine was the most abundant polyamine found in B. buxifolia tissues, 14- to 18-fold more than spermidine and spermine, respectively. Therefore, these compounds were used to define the rooting phases: an induction phase (0 to 4–7 days) followed by an expression phase (4–7 to 28 days). The observed changes in the biochemical markers could be correlated with microscopic and macroscopic tissue observations in the microshoots, and the time course of rooting percentage. Successive culture media can be developed including polyamines, or other compounds and environmental conditions, which positively modify the studied biochemical markers behaviour.
Fil: Arena, Miriam Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Martínez Pastur, Guillermo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Eliasco, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description Berberis buxifolia is a Patagonian shrub with great economic potential for tinctorial, pharmacological, and food industries. Clonal propagation is possible through in vitro culture and is also useful for metabolite production. However, this species is difficult to root, and to improve this, more knowledge of rhizogenesis processes is needed. Polyamines and peroxidases are useful biochemical markers during analysis of rooting phases for correlation with tissue morphological changes. Therefore, endogenous polyamine (putrescine, spermidine, spermine) changes, peroxidase activity evolution, and morphological development were studied to characterise the in vitro rhizogenesis of microshoots of B. buxifolia and, thus, to define the rooting phases. Polyamine and peroxidase changed significantly during the rooting period, and had opposite behaviours which were directly related to the IBA media content. The lower polyamine concentration and the higher peroxidase activity were found in a treatment with a dark period during the first four days and with IBA in the culture medium. Putrescine was the most abundant polyamine found in B. buxifolia tissues, 14- to 18-fold more than spermidine and spermine, respectively. Therefore, these compounds were used to define the rooting phases: an induction phase (0 to 4–7 days) followed by an expression phase (4–7 to 28 days). The observed changes in the biochemical markers could be correlated with microscopic and macroscopic tissue observations in the microshoots, and the time course of rooting percentage. Successive culture media can be developed including polyamines, or other compounds and environmental conditions, which positively modify the studied biochemical markers behaviour.
publishDate 2003
dc.date.none.fl_str_mv 2003-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33625
Arena, Miriam Elisabet; Curvetto, Nestor Raul; Zappacosta, Diego Carlos; Benavides, Maria Patricia; Martínez Pastur, Guillermo José; Eliasco, Eleonora; et al.; Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia; Rsnz Publishing; New Zealand Journal of Botany; 41; 3; 12-2003; 475-485
0028-825X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33625
identifier_str_mv Arena, Miriam Elisabet; Curvetto, Nestor Raul; Zappacosta, Diego Carlos; Benavides, Maria Patricia; Martínez Pastur, Guillermo José; Eliasco, Eleonora; et al.; Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia; Rsnz Publishing; New Zealand Journal of Botany; 41; 3; 12-2003; 475-485
0028-825X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/0028825X.2003.9512864
info:eu-repo/semantics/altIdentifier/doi/10.1080/0028825X.2003.9512864
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Rsnz Publishing
publisher.none.fl_str_mv Rsnz Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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