Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia

Autores
Arena, Miriam Elisabet; Martínez Pastur, Guillermo José; Benavides, Maria Patricia; Curvetto, Nestor Raul
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although Berberis buxifolia has been reported to propagate in vitro, rooting is difficult to achieve during subcultures, with reduced quantity and quality of roots, as is the case with many other woody species. Several cofactors of rhizogenesis have been proposed, and the role of polyamines in rooting has recently acquired relevance. Polyamines can stimulate or inhibit microshoot rooting depending on the type and concentration of polyamine and rooting phase, while polyamine inhibitors can improve rooting in some cases. A study of the in vitro rooting of B. buxifolia using polyamines and a mix of polyamine inhibitors in a two-step culture medium is described here, in which a new successive rooting medium was successfully implemented. While polyamine inhibitors sometimes improved rooting, nutrient medium containing a low polyamine concentration (i.e., 1 μM) enhanced rooting compared with the control medium. The best micro-shoot rooting response resulted from the addition of 1 μM spermidine during the expression phase in the absence of inhibitors in both rooting phases. Overall, incorporating polyamine in the successive media enhances the quality and quantity of roots in B. buxifolia, thus confirming their role during the induction and expression of the radicle primordia differentiation.
Fil: Arena, Miriam Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Martínez Pastur, Guillermo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Curvetto, Nestor Raul. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Berberis Buxifolia
Calafate
Micropropagation
Patagonia
Polyamine Inhibitors
Rhizogenesis
Root System Quality
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55768
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifoliaArena, Miriam ElisabetMartínez Pastur, Guillermo JoséBenavides, Maria PatriciaCurvetto, Nestor RaulBerberis BuxifoliaCalafateMicropropagationPatagoniaPolyamine InhibitorsRhizogenesisRoot System Qualityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although Berberis buxifolia has been reported to propagate in vitro, rooting is difficult to achieve during subcultures, with reduced quantity and quality of roots, as is the case with many other woody species. Several cofactors of rhizogenesis have been proposed, and the role of polyamines in rooting has recently acquired relevance. Polyamines can stimulate or inhibit microshoot rooting depending on the type and concentration of polyamine and rooting phase, while polyamine inhibitors can improve rooting in some cases. A study of the in vitro rooting of B. buxifolia using polyamines and a mix of polyamine inhibitors in a two-step culture medium is described here, in which a new successive rooting medium was successfully implemented. While polyamine inhibitors sometimes improved rooting, nutrient medium containing a low polyamine concentration (i.e., 1 μM) enhanced rooting compared with the control medium. The best micro-shoot rooting response resulted from the addition of 1 μM spermidine during the expression phase in the absence of inhibitors in both rooting phases. Overall, incorporating polyamine in the successive media enhances the quality and quantity of roots in B. buxifolia, thus confirming their role during the induction and expression of the radicle primordia differentiation.Fil: Arena, Miriam Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Martínez Pastur, Guillermo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Curvetto, Nestor Raul. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaRoyal Society of New Zealand2005-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55768Arena, Miriam Elisabet; Martínez Pastur, Guillermo José; Benavides, Maria Patricia; Curvetto, Nestor Raul; Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia; Royal Society of New Zealand; New Zealand Journal of Botany; 43; 2; 12-2005; 373-3800028-825XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/0028825X.2005.9512962info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/0028825X.2005.9512962info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:24Zoai:ri.conicet.gov.ar:11336/55768instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:25.146CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
title Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
spellingShingle Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
Arena, Miriam Elisabet
Berberis Buxifolia
Calafate
Micropropagation
Patagonia
Polyamine Inhibitors
Rhizogenesis
Root System Quality
title_short Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
title_full Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
title_fullStr Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
title_full_unstemmed Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
title_sort Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
dc.creator.none.fl_str_mv Arena, Miriam Elisabet
Martínez Pastur, Guillermo José
Benavides, Maria Patricia
Curvetto, Nestor Raul
author Arena, Miriam Elisabet
author_facet Arena, Miriam Elisabet
Martínez Pastur, Guillermo José
Benavides, Maria Patricia
Curvetto, Nestor Raul
author_role author
author2 Martínez Pastur, Guillermo José
Benavides, Maria Patricia
Curvetto, Nestor Raul
author2_role author
author
author
dc.subject.none.fl_str_mv Berberis Buxifolia
Calafate
Micropropagation
Patagonia
Polyamine Inhibitors
Rhizogenesis
Root System Quality
topic Berberis Buxifolia
Calafate
Micropropagation
Patagonia
Polyamine Inhibitors
Rhizogenesis
Root System Quality
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although Berberis buxifolia has been reported to propagate in vitro, rooting is difficult to achieve during subcultures, with reduced quantity and quality of roots, as is the case with many other woody species. Several cofactors of rhizogenesis have been proposed, and the role of polyamines in rooting has recently acquired relevance. Polyamines can stimulate or inhibit microshoot rooting depending on the type and concentration of polyamine and rooting phase, while polyamine inhibitors can improve rooting in some cases. A study of the in vitro rooting of B. buxifolia using polyamines and a mix of polyamine inhibitors in a two-step culture medium is described here, in which a new successive rooting medium was successfully implemented. While polyamine inhibitors sometimes improved rooting, nutrient medium containing a low polyamine concentration (i.e., 1 μM) enhanced rooting compared with the control medium. The best micro-shoot rooting response resulted from the addition of 1 μM spermidine during the expression phase in the absence of inhibitors in both rooting phases. Overall, incorporating polyamine in the successive media enhances the quality and quantity of roots in B. buxifolia, thus confirming their role during the induction and expression of the radicle primordia differentiation.
Fil: Arena, Miriam Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Martínez Pastur, Guillermo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Curvetto, Nestor Raul. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Although Berberis buxifolia has been reported to propagate in vitro, rooting is difficult to achieve during subcultures, with reduced quantity and quality of roots, as is the case with many other woody species. Several cofactors of rhizogenesis have been proposed, and the role of polyamines in rooting has recently acquired relevance. Polyamines can stimulate or inhibit microshoot rooting depending on the type and concentration of polyamine and rooting phase, while polyamine inhibitors can improve rooting in some cases. A study of the in vitro rooting of B. buxifolia using polyamines and a mix of polyamine inhibitors in a two-step culture medium is described here, in which a new successive rooting medium was successfully implemented. While polyamine inhibitors sometimes improved rooting, nutrient medium containing a low polyamine concentration (i.e., 1 μM) enhanced rooting compared with the control medium. The best micro-shoot rooting response resulted from the addition of 1 μM spermidine during the expression phase in the absence of inhibitors in both rooting phases. Overall, incorporating polyamine in the successive media enhances the quality and quantity of roots in B. buxifolia, thus confirming their role during the induction and expression of the radicle primordia differentiation.
publishDate 2005
dc.date.none.fl_str_mv 2005-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55768
Arena, Miriam Elisabet; Martínez Pastur, Guillermo José; Benavides, Maria Patricia; Curvetto, Nestor Raul; Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia; Royal Society of New Zealand; New Zealand Journal of Botany; 43; 2; 12-2005; 373-380
0028-825X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55768
identifier_str_mv Arena, Miriam Elisabet; Martínez Pastur, Guillermo José; Benavides, Maria Patricia; Curvetto, Nestor Raul; Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia; Royal Society of New Zealand; New Zealand Journal of Botany; 43; 2; 12-2005; 373-380
0028-825X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/0028825X.2005.9512962
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/0028825X.2005.9512962
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of New Zealand
publisher.none.fl_str_mv Royal Society of New Zealand
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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