Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)

Autores
Sauka, Diego Herman; Monella, Rodrigo Horacio; Benintende, Graciela Beatriz
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this work was to establish and test the induced-feeding bioassay in order to detect Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera: Coccinellidae). Larvae were induced to swallow high concentrations of spore-crystal suspensions of seven exotic and 30 Argentine B. thuringiensis strains. The great majority of strains showed no toxicity to E. paenulata larvae, and observed mortality was lower than 30%. Induced-feeding bioassay is feasible, and should be used for prospecting strains that produce right combinations of Cry proteins needed to an efficient pest control.
O objetivo deste trabalho foi estabelecer e testar o bioensaio de alimentação induzida para a determinação da atividade inseticida de Bacillus thuringiensis contra larvas de Epilachna paenulata (Coleoptera: Coccinellidae). As larvas foram induzidas a ingerir concentrações elevadas de suspensões esporo-cristal de 7 cepas exóticas e 30 argentinas de B. thuringiensis. A maioria das cepas não apresentou toxicidade a larvas de E. paenulata, com mortalidade inferior a 30%. O bioensaio de alimentação induzida se mostrou efetivo, e pode ser usado para identificar cepas que produzam combinações adequadas de proteínas Cry, necessárias para o controle de pragas.
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Monella, Rodrigo Horacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Materia
BIOLOGICAL CONTROL
COCCINELLIDAE
CUCURBITACEAE
LADYBIRD BEETLE
SPORE-CRYSTAL TOXICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189286
Acceder
id CONICETDig_8bdbb589c8e5ed10139af4ad81a3998f
oai_identifier_str oai:ri.conicet.gov.ar:11336/189286
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)Bioensaios de alimentação induzida para determinar a atividade inseticida de Bacillus thuringiensis contra Epilachna paenulata (Coleoptera)Sauka, Diego HermanMonella, Rodrigo HoracioBenintende, Graciela BeatrizBIOLOGICAL CONTROLCOCCINELLIDAECUCURBITACEAELADYBIRD BEETLESPORE-CRYSTAL TOXICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The objective of this work was to establish and test the induced-feeding bioassay in order to detect Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera: Coccinellidae). Larvae were induced to swallow high concentrations of spore-crystal suspensions of seven exotic and 30 Argentine B. thuringiensis strains. The great majority of strains showed no toxicity to E. paenulata larvae, and observed mortality was lower than 30%. Induced-feeding bioassay is feasible, and should be used for prospecting strains that produce right combinations of Cry proteins needed to an efficient pest control.O objetivo deste trabalho foi estabelecer e testar o bioensaio de alimentação induzida para a determinação da atividade inseticida de Bacillus thuringiensis contra larvas de Epilachna paenulata (Coleoptera: Coccinellidae). As larvas foram induzidas a ingerir concentrações elevadas de suspensões esporo-cristal de 7 cepas exóticas e 30 argentinas de B. thuringiensis. A maioria das cepas não apresentou toxicidade a larvas de E. paenulata, com mortalidade inferior a 30%. O bioensaio de alimentação induzida se mostrou efetivo, e pode ser usado para identificar cepas que produzam combinações adequadas de proteínas Cry, necessárias para o controle de pragas.Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Monella, Rodrigo Horacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaEmpresa Brasileira de Pesquisa Agropecuária2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189286Sauka, Diego Herman; Monella, Rodrigo Horacio; Benintende, Graciela Beatriz; Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera); Empresa Brasileira de Pesquisa Agropecuária; Pesquisa Agropecuária Brasileira; 45; 4; 4-2010; 430-4320100-204X1678-3921CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/pab/a/5j95FxtWw3hjZwz9Ptqdr8b/?lang=eninfo:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-204X2010000400012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:42Zoai:ri.conicet.gov.ar:11336/189286instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:42.41CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)
Bioensaios de alimentação induzida para determinar a atividade inseticida de Bacillus thuringiensis contra Epilachna paenulata (Coleoptera)
title Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)
spellingShingle Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)
Sauka, Diego Herman
BIOLOGICAL CONTROL
COCCINELLIDAE
CUCURBITACEAE
LADYBIRD BEETLE
SPORE-CRYSTAL TOXICITY
title_short Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)
title_full Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)
title_fullStr Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)
title_full_unstemmed Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)
title_sort Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)
dc.creator.none.fl_str_mv Sauka, Diego Herman
Monella, Rodrigo Horacio
Benintende, Graciela Beatriz
author Sauka, Diego Herman
author_facet Sauka, Diego Herman
Monella, Rodrigo Horacio
Benintende, Graciela Beatriz
author_role author
author2 Monella, Rodrigo Horacio
Benintende, Graciela Beatriz
author2_role author
author
dc.subject.none.fl_str_mv BIOLOGICAL CONTROL
COCCINELLIDAE
CUCURBITACEAE
LADYBIRD BEETLE
SPORE-CRYSTAL TOXICITY
topic BIOLOGICAL CONTROL
COCCINELLIDAE
CUCURBITACEAE
LADYBIRD BEETLE
SPORE-CRYSTAL TOXICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The objective of this work was to establish and test the induced-feeding bioassay in order to detect Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera: Coccinellidae). Larvae were induced to swallow high concentrations of spore-crystal suspensions of seven exotic and 30 Argentine B. thuringiensis strains. The great majority of strains showed no toxicity to E. paenulata larvae, and observed mortality was lower than 30%. Induced-feeding bioassay is feasible, and should be used for prospecting strains that produce right combinations of Cry proteins needed to an efficient pest control.
O objetivo deste trabalho foi estabelecer e testar o bioensaio de alimentação induzida para a determinação da atividade inseticida de Bacillus thuringiensis contra larvas de Epilachna paenulata (Coleoptera: Coccinellidae). As larvas foram induzidas a ingerir concentrações elevadas de suspensões esporo-cristal de 7 cepas exóticas e 30 argentinas de B. thuringiensis. A maioria das cepas não apresentou toxicidade a larvas de E. paenulata, com mortalidade inferior a 30%. O bioensaio de alimentação induzida se mostrou efetivo, e pode ser usado para identificar cepas que produzam combinações adequadas de proteínas Cry, necessárias para o controle de pragas.
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Monella, Rodrigo Horacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
description The objective of this work was to establish and test the induced-feeding bioassay in order to detect Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera: Coccinellidae). Larvae were induced to swallow high concentrations of spore-crystal suspensions of seven exotic and 30 Argentine B. thuringiensis strains. The great majority of strains showed no toxicity to E. paenulata larvae, and observed mortality was lower than 30%. Induced-feeding bioassay is feasible, and should be used for prospecting strains that produce right combinations of Cry proteins needed to an efficient pest control.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189286
Sauka, Diego Herman; Monella, Rodrigo Horacio; Benintende, Graciela Beatriz; Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera); Empresa Brasileira de Pesquisa Agropecuária; Pesquisa Agropecuária Brasileira; 45; 4; 4-2010; 430-432
0100-204X
1678-3921
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189286
identifier_str_mv Sauka, Diego Herman; Monella, Rodrigo Horacio; Benintende, Graciela Beatriz; Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera); Empresa Brasileira de Pesquisa Agropecuária; Pesquisa Agropecuária Brasileira; 45; 4; 4-2010; 430-432
0100-204X
1678-3921
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/pab/a/5j95FxtWw3hjZwz9Ptqdr8b/?lang=en
info:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-204X2010000400012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária
publisher.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613814448816128
score 13.070432

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