Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles

Autores
Álamo, Patricia; Pallarès, Victor; Céspedes, María Virtudes; Falgàs, Aïda; Sanchez, Julieta Maria; Serna, Naroa; Sánchez-García, Laura; Voltà-Duràn, Eric; Morris, Gordon A.; Sánchez-Chardi, Alejandro; Casanova, Isolda; Mangues, Ramón; Vazquez, Esther; Villaverde Corrales, Antonio; Unzueta, Ugutz
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fluorescent dye labeling is a common strategy to analyze the fate of administered nanoparticles in living organisms. However, to which extent the labeling processes can alter the original nanoparticle biodistribution has been so far neglected. In this work, two widely used fluorescent dye molecules, namely, ATTO488 (ATTO) and Sulfo‐Cy5 (S‐Cy5), have been covalently attached to a well‐characterized CXCR4‐targeted self‐assembling protein nanoparticle (known as T22‐GFP‐H6). The biodistribution of labeled T22‐GFP‐H6‐ATTO and T22‐GFP‐H6‐S‐Cy5 nanoparticles has been then compared to that of the non‐labeled nanoparticle in different CXCR4+ tumor mouse models. We observed that while parental T22‐GFP‐H6 nanoparticles accumulated mostly and specifically in CXCR4+ tumor cells, labeled T22‐GFP‐H6‐ATTO and T22‐GFP‐H6‐S‐Cy5 nanoparticles showed a dramatic change in the biodistribution pattern, accumulating in non‐target organs such as liver or kidney while reducing tumor targeting capacity. Therefore, the use of such labeling molecules should be avoided in target and non‐target tissue uptake studies during the design and development of targeted nanoscale drug delivery systems, since their effect over the fate of the nanomaterial can lead to considerable miss‐interpretations of the actual nanoparticle biodistribution.
Fil: Álamo, Patricia. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; España
Fil: Pallarès, Victor. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; España
Fil: Céspedes, María Virtudes. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; España
Fil: Falgàs, Aïda. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; España
Fil: Sanchez, Julieta Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universitat Autònoma de Barcelona; España. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Ciencias y Tecnología de los Alimentos; Argentina
Fil: Serna, Naroa. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Sánchez-García, Laura. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Voltà-Duràn, Eric. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Morris, Gordon A.. University of Huddersfiel; Reino Unido
Fil: Sánchez-Chardi, Alejandro. Universitat Autònoma de Barcelona; España
Fil: Casanova, Isolda. Biomedical Research Institute Sant Pau; España. Centro de Investigacion Biomedica En Red.; España
Fil: Mangues, Ramón. Biomedical Research Institute Sant Pau; España. Centro de Investigacion Biomedica En Red.; España
Fil: Vazquez, Esther. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Villaverde Corrales, Antonio. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Unzueta, Ugutz. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Materia
PROTEIN NANOMATERIALS
FUNCTIONAL MATERIALS
SELF‐ASSEMBLING NANOPARTICLES
FLUORESCENT LABELING
BIODISTRIBUTION
TARGETING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/119981
Acceder
id CONICETDig_8bc5fe9d8f89716fe9aec78322f86478
oai_identifier_str oai:ri.conicet.gov.ar:11336/119981
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticlesÁlamo, PatriciaPallarès, VictorCéspedes, María VirtudesFalgàs, AïdaSanchez, Julieta MariaSerna, NaroaSánchez-García, LauraVoltà-Duràn, EricMorris, Gordon A.Sánchez-Chardi, AlejandroCasanova, IsoldaMangues, RamónVazquez, EstherVillaverde Corrales, AntonioUnzueta, UgutzPROTEIN NANOMATERIALSFUNCTIONAL MATERIALSSELF‐ASSEMBLING NANOPARTICLESFLUORESCENT LABELINGBIODISTRIBUTIONTARGETINGhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Fluorescent dye labeling is a common strategy to analyze the fate of administered nanoparticles in living organisms. However, to which extent the labeling processes can alter the original nanoparticle biodistribution has been so far neglected. In this work, two widely used fluorescent dye molecules, namely, ATTO488 (ATTO) and Sulfo‐Cy5 (S‐Cy5), have been covalently attached to a well‐characterized CXCR4‐targeted self‐assembling protein nanoparticle (known as T22‐GFP‐H6). The biodistribution of labeled T22‐GFP‐H6‐ATTO and T22‐GFP‐H6‐S‐Cy5 nanoparticles has been then compared to that of the non‐labeled nanoparticle in different CXCR4+ tumor mouse models. We observed that while parental T22‐GFP‐H6 nanoparticles accumulated mostly and specifically in CXCR4+ tumor cells, labeled T22‐GFP‐H6‐ATTO and T22‐GFP‐H6‐S‐Cy5 nanoparticles showed a dramatic change in the biodistribution pattern, accumulating in non‐target organs such as liver or kidney while reducing tumor targeting capacity. Therefore, the use of such labeling molecules should be avoided in target and non‐target tissue uptake studies during the design and development of targeted nanoscale drug delivery systems, since their effect over the fate of the nanomaterial can lead to considerable miss‐interpretations of the actual nanoparticle biodistribution.Fil: Álamo, Patricia. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; EspañaFil: Pallarès, Victor. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; EspañaFil: Céspedes, María Virtudes. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; EspañaFil: Falgàs, Aïda. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; EspañaFil: Sanchez, Julieta Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universitat Autònoma de Barcelona; España. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Ciencias y Tecnología de los Alimentos; ArgentinaFil: Serna, Naroa. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; EspañaFil: Sánchez-García, Laura. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; EspañaFil: Voltà-Duràn, Eric. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; EspañaFil: Morris, Gordon A.. University of Huddersfiel; Reino UnidoFil: Sánchez-Chardi, Alejandro. Universitat Autònoma de Barcelona; EspañaFil: Casanova, Isolda. Biomedical Research Institute Sant Pau; España. Centro de Investigacion Biomedica En Red.; EspañaFil: Mangues, Ramón. Biomedical Research Institute Sant Pau; España. Centro de Investigacion Biomedica En Red.; EspañaFil: Vazquez, Esther. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; EspañaFil: Villaverde Corrales, Antonio. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; EspañaFil: Unzueta, Ugutz. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; EspañaMDPI AG2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/119981Álamo, Patricia; Pallarès, Victor; Céspedes, María Virtudes; Falgàs, Aïda; Sanchez, Julieta Maria; et al.; Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles; MDPI AG; Pharmaceutics; 12; 11; 11-2020; 1-181999-4923CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/12/11/1004info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics12111004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:59Zoai:ri.conicet.gov.ar:11336/119981instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:59.985CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles
title Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles
spellingShingle Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles
Álamo, Patricia
PROTEIN NANOMATERIALS
FUNCTIONAL MATERIALS
SELF‐ASSEMBLING NANOPARTICLES
FLUORESCENT LABELING
BIODISTRIBUTION
TARGETING
title_short Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles
title_full Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles
title_fullStr Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles
title_full_unstemmed Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles
title_sort Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles
dc.creator.none.fl_str_mv Álamo, Patricia
Pallarès, Victor
Céspedes, María Virtudes
Falgàs, Aïda
Sanchez, Julieta Maria
Serna, Naroa
Sánchez-García, Laura
Voltà-Duràn, Eric
Morris, Gordon A.
Sánchez-Chardi, Alejandro
Casanova, Isolda
Mangues, Ramón
Vazquez, Esther
Villaverde Corrales, Antonio
Unzueta, Ugutz
author Álamo, Patricia
author_facet Álamo, Patricia
Pallarès, Victor
Céspedes, María Virtudes
Falgàs, Aïda
Sanchez, Julieta Maria
Serna, Naroa
Sánchez-García, Laura
Voltà-Duràn, Eric
Morris, Gordon A.
Sánchez-Chardi, Alejandro
Casanova, Isolda
Mangues, Ramón
Vazquez, Esther
Villaverde Corrales, Antonio
Unzueta, Ugutz
author_role author
author2 Pallarès, Victor
Céspedes, María Virtudes
Falgàs, Aïda
Sanchez, Julieta Maria
Serna, Naroa
Sánchez-García, Laura
Voltà-Duràn, Eric
Morris, Gordon A.
Sánchez-Chardi, Alejandro
Casanova, Isolda
Mangues, Ramón
Vazquez, Esther
Villaverde Corrales, Antonio
Unzueta, Ugutz
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PROTEIN NANOMATERIALS
FUNCTIONAL MATERIALS
SELF‐ASSEMBLING NANOPARTICLES
FLUORESCENT LABELING
BIODISTRIBUTION
TARGETING
topic PROTEIN NANOMATERIALS
FUNCTIONAL MATERIALS
SELF‐ASSEMBLING NANOPARTICLES
FLUORESCENT LABELING
BIODISTRIBUTION
TARGETING
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Fluorescent dye labeling is a common strategy to analyze the fate of administered nanoparticles in living organisms. However, to which extent the labeling processes can alter the original nanoparticle biodistribution has been so far neglected. In this work, two widely used fluorescent dye molecules, namely, ATTO488 (ATTO) and Sulfo‐Cy5 (S‐Cy5), have been covalently attached to a well‐characterized CXCR4‐targeted self‐assembling protein nanoparticle (known as T22‐GFP‐H6). The biodistribution of labeled T22‐GFP‐H6‐ATTO and T22‐GFP‐H6‐S‐Cy5 nanoparticles has been then compared to that of the non‐labeled nanoparticle in different CXCR4+ tumor mouse models. We observed that while parental T22‐GFP‐H6 nanoparticles accumulated mostly and specifically in CXCR4+ tumor cells, labeled T22‐GFP‐H6‐ATTO and T22‐GFP‐H6‐S‐Cy5 nanoparticles showed a dramatic change in the biodistribution pattern, accumulating in non‐target organs such as liver or kidney while reducing tumor targeting capacity. Therefore, the use of such labeling molecules should be avoided in target and non‐target tissue uptake studies during the design and development of targeted nanoscale drug delivery systems, since their effect over the fate of the nanomaterial can lead to considerable miss‐interpretations of the actual nanoparticle biodistribution.
Fil: Álamo, Patricia. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; España
Fil: Pallarès, Victor. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; España
Fil: Céspedes, María Virtudes. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; España
Fil: Falgàs, Aïda. Centro de Investigacion Biomedica En Red.; España. Biomedical Research Institute Sant Pau; España
Fil: Sanchez, Julieta Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universitat Autònoma de Barcelona; España. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Ciencias y Tecnología de los Alimentos; Argentina
Fil: Serna, Naroa. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Sánchez-García, Laura. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Voltà-Duràn, Eric. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Morris, Gordon A.. University of Huddersfiel; Reino Unido
Fil: Sánchez-Chardi, Alejandro. Universitat Autònoma de Barcelona; España
Fil: Casanova, Isolda. Biomedical Research Institute Sant Pau; España. Centro de Investigacion Biomedica En Red.; España
Fil: Mangues, Ramón. Biomedical Research Institute Sant Pau; España. Centro de Investigacion Biomedica En Red.; España
Fil: Vazquez, Esther. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Villaverde Corrales, Antonio. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
Fil: Unzueta, Ugutz. Universitat Autònoma de Barcelona; España. Centro de Investigacion Biomedica En Red.; España
description Fluorescent dye labeling is a common strategy to analyze the fate of administered nanoparticles in living organisms. However, to which extent the labeling processes can alter the original nanoparticle biodistribution has been so far neglected. In this work, two widely used fluorescent dye molecules, namely, ATTO488 (ATTO) and Sulfo‐Cy5 (S‐Cy5), have been covalently attached to a well‐characterized CXCR4‐targeted self‐assembling protein nanoparticle (known as T22‐GFP‐H6). The biodistribution of labeled T22‐GFP‐H6‐ATTO and T22‐GFP‐H6‐S‐Cy5 nanoparticles has been then compared to that of the non‐labeled nanoparticle in different CXCR4+ tumor mouse models. We observed that while parental T22‐GFP‐H6 nanoparticles accumulated mostly and specifically in CXCR4+ tumor cells, labeled T22‐GFP‐H6‐ATTO and T22‐GFP‐H6‐S‐Cy5 nanoparticles showed a dramatic change in the biodistribution pattern, accumulating in non‐target organs such as liver or kidney while reducing tumor targeting capacity. Therefore, the use of such labeling molecules should be avoided in target and non‐target tissue uptake studies during the design and development of targeted nanoscale drug delivery systems, since their effect over the fate of the nanomaterial can lead to considerable miss‐interpretations of the actual nanoparticle biodistribution.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/119981
Álamo, Patricia; Pallarès, Victor; Céspedes, María Virtudes; Falgàs, Aïda; Sanchez, Julieta Maria; et al.; Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles; MDPI AG; Pharmaceutics; 12; 11; 11-2020; 1-18
1999-4923
CONICET Digital
CONICET
url http://hdl.handle.net/11336/119981
identifier_str_mv Álamo, Patricia; Pallarès, Victor; Céspedes, María Virtudes; Falgàs, Aïda; Sanchez, Julieta Maria; et al.; Fluorescent dye labeling changes the biodistribution of tumor‐targeted nanoparticles; MDPI AG; Pharmaceutics; 12; 11; 11-2020; 1-18
1999-4923
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/12/11/1004
info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics12111004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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