Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72

Autores
Contreras Molina, Maria Isabel; Otegui, Marisa; Petruccelli, Silvana
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Storage proteins are transported to a special storage compartments in seeds by Golgi dependent or independent pathways depending on the plant species. The aim of this work was to study the sunflower storage protein transport pathway and to identify component of the sorting machinery. Immature sunflower seeds were analyzed by subcellular fractionation (using percoll and sucrose gradients) and electron microscopy. The vesicles isolated with percoll, have precursors of 11S globulins, a-TIP, d-TIP, BiP, and two bands that have homology to the pumpkin vacuolar sorting receptor PV72. Sucrose isolated vesicles have the same composition than percoll ones, except for the lack of BiP and the presence of only one protein that has reactivity with pea VSR BP80. Electronic micrographies of developing seeds show that the formation of electron dense aggregates starts in the endoplasmic reticulum, and that these aggregates are very abundant in the trans-Golgi apparatus, where release of dense vesicles happens. These vesicles contain a homolog of PV72 in their membranes. Storage proteins are also detected in multivesicular bodies whose membranes have reactivity with PV72 serum. All these results indicated that sunflower storage proteins are transported to protein storage vacuoles by a Golgi dependent pathway in a process in which homologous of PV72 are involved.
Fil: Contreras Molina, Maria Isabel. Michigan State University; Estados Unidos
Fil: Otegui, Marisa. University of Wisconsin; Estados Unidos
Fil: Petruccelli, Silvana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Materia
Developing sunflower seeds
Storage proteins
Transport pathway
Vacuolar sorting
Vacuolar sorting receptor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/115294
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/115294
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72Contreras Molina, Maria IsabelOtegui, MarisaPetruccelli, SilvanaDeveloping sunflower seedsStorage proteinsTransport pathwayVacuolar sortingVacuolar sorting receptorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Storage proteins are transported to a special storage compartments in seeds by Golgi dependent or independent pathways depending on the plant species. The aim of this work was to study the sunflower storage protein transport pathway and to identify component of the sorting machinery. Immature sunflower seeds were analyzed by subcellular fractionation (using percoll and sucrose gradients) and electron microscopy. The vesicles isolated with percoll, have precursors of 11S globulins, a-TIP, d-TIP, BiP, and two bands that have homology to the pumpkin vacuolar sorting receptor PV72. Sucrose isolated vesicles have the same composition than percoll ones, except for the lack of BiP and the presence of only one protein that has reactivity with pea VSR BP80. Electronic micrographies of developing seeds show that the formation of electron dense aggregates starts in the endoplasmic reticulum, and that these aggregates are very abundant in the trans-Golgi apparatus, where release of dense vesicles happens. These vesicles contain a homolog of PV72 in their membranes. Storage proteins are also detected in multivesicular bodies whose membranes have reactivity with PV72 serum. All these results indicated that sunflower storage proteins are transported to protein storage vacuoles by a Golgi dependent pathway in a process in which homologous of PV72 are involved.Fil: Contreras Molina, Maria Isabel. Michigan State University; Estados UnidosFil: Otegui, Marisa. University of Wisconsin; Estados UnidosFil: Petruccelli, Silvana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaPontificia Universidad Católica de Valparaíso2006-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/115294Contreras Molina, Maria Isabel; Otegui, Marisa; Petruccelli, Silvana; Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 9; 3; 6-2006; 1-50717-3458CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://tinyurl.com/yyqqvxf5info:eu-repo/semantics/altIdentifier/doi/10.2225/vol9-issue3-fulltext-21info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:52Zoai:ri.conicet.gov.ar:11336/115294instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:52.939CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72
title Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72
spellingShingle Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72
Contreras Molina, Maria Isabel
Developing sunflower seeds
Storage proteins
Transport pathway
Vacuolar sorting
Vacuolar sorting receptor
title_short Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72
title_full Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72
title_fullStr Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72
title_full_unstemmed Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72
title_sort Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72
dc.creator.none.fl_str_mv Contreras Molina, Maria Isabel
Otegui, Marisa
Petruccelli, Silvana
author Contreras Molina, Maria Isabel
author_facet Contreras Molina, Maria Isabel
Otegui, Marisa
Petruccelli, Silvana
author_role author
author2 Otegui, Marisa
Petruccelli, Silvana
author2_role author
author
dc.subject.none.fl_str_mv Developing sunflower seeds
Storage proteins
Transport pathway
Vacuolar sorting
Vacuolar sorting receptor
topic Developing sunflower seeds
Storage proteins
Transport pathway
Vacuolar sorting
Vacuolar sorting receptor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Storage proteins are transported to a special storage compartments in seeds by Golgi dependent or independent pathways depending on the plant species. The aim of this work was to study the sunflower storage protein transport pathway and to identify component of the sorting machinery. Immature sunflower seeds were analyzed by subcellular fractionation (using percoll and sucrose gradients) and electron microscopy. The vesicles isolated with percoll, have precursors of 11S globulins, a-TIP, d-TIP, BiP, and two bands that have homology to the pumpkin vacuolar sorting receptor PV72. Sucrose isolated vesicles have the same composition than percoll ones, except for the lack of BiP and the presence of only one protein that has reactivity with pea VSR BP80. Electronic micrographies of developing seeds show that the formation of electron dense aggregates starts in the endoplasmic reticulum, and that these aggregates are very abundant in the trans-Golgi apparatus, where release of dense vesicles happens. These vesicles contain a homolog of PV72 in their membranes. Storage proteins are also detected in multivesicular bodies whose membranes have reactivity with PV72 serum. All these results indicated that sunflower storage proteins are transported to protein storage vacuoles by a Golgi dependent pathway in a process in which homologous of PV72 are involved.
Fil: Contreras Molina, Maria Isabel. Michigan State University; Estados Unidos
Fil: Otegui, Marisa. University of Wisconsin; Estados Unidos
Fil: Petruccelli, Silvana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
description Storage proteins are transported to a special storage compartments in seeds by Golgi dependent or independent pathways depending on the plant species. The aim of this work was to study the sunflower storage protein transport pathway and to identify component of the sorting machinery. Immature sunflower seeds were analyzed by subcellular fractionation (using percoll and sucrose gradients) and electron microscopy. The vesicles isolated with percoll, have precursors of 11S globulins, a-TIP, d-TIP, BiP, and two bands that have homology to the pumpkin vacuolar sorting receptor PV72. Sucrose isolated vesicles have the same composition than percoll ones, except for the lack of BiP and the presence of only one protein that has reactivity with pea VSR BP80. Electronic micrographies of developing seeds show that the formation of electron dense aggregates starts in the endoplasmic reticulum, and that these aggregates are very abundant in the trans-Golgi apparatus, where release of dense vesicles happens. These vesicles contain a homolog of PV72 in their membranes. Storage proteins are also detected in multivesicular bodies whose membranes have reactivity with PV72 serum. All these results indicated that sunflower storage proteins are transported to protein storage vacuoles by a Golgi dependent pathway in a process in which homologous of PV72 are involved.
publishDate 2006
dc.date.none.fl_str_mv 2006-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/115294
Contreras Molina, Maria Isabel; Otegui, Marisa; Petruccelli, Silvana; Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 9; 3; 6-2006; 1-5
0717-3458
CONICET Digital
CONICET
url http://hdl.handle.net/11336/115294
identifier_str_mv Contreras Molina, Maria Isabel; Otegui, Marisa; Petruccelli, Silvana; Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 9; 3; 6-2006; 1-5
0717-3458
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://tinyurl.com/yyqqvxf5
info:eu-repo/semantics/altIdentifier/doi/10.2225/vol9-issue3-fulltext-21
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pontificia Universidad Católica de Valparaíso
publisher.none.fl_str_mv Pontificia Universidad Católica de Valparaíso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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