Vacuolar Targeting and Characterization of Recombinant Antibodies

Autores
Ocampo, Carolina Gabriela; Petruccelli, Silvana; Pereira, Cláudia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
parte de libro
Estado
versión publicada
Descripción
Plant-based platforms are extensively use for the expression of recombinant proteins, including monoclonal antibodies (mAbs). Generally, immunoglobulins (Igs) are sorted to the apoplast, which is often afflicted with intense proteolysis. Here, we describe methods to transiently express mAbs sorted to central vacuole in Nicotiana benthamiana leaves and to characterize the obtained IgG. Central vacuole is an appropriate compartment for the efficient production of Abs, consequently vacuolar sorting should be considered as an alternative strategy to obtain high protein yields.
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Biología
Immunoglobulin
N-glycosylation
Vacuolar sorting signals
Secretory pathway
Vacuolar transport
Molecular farming
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/138728
Acceder
id SEDICI_8017247b4ac83a4dc692506266c865c4
oai_identifier_str oai:sedici.unlp.edu.ar:10915/138728
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Vacuolar Targeting and Characterization of Recombinant AntibodiesOcampo, Carolina GabrielaPetruccelli, SilvanaPereira, CláudiaCiencias ExactasBiologíaImmunoglobulinN-glycosylationVacuolar sorting signalsSecretory pathwayVacuolar transportMolecular farmingPlant-based platforms are extensively use for the expression of recombinant proteins, including monoclonal antibodies (mAbs). Generally, immunoglobulins (Igs) are sorted to the apoplast, which is often afflicted with intense proteolysis. Here, we describe methods to transiently express mAbs sorted to central vacuole in Nicotiana benthamiana leaves and to characterize the obtained IgG. Central vacuole is an appropriate compartment for the efficient production of Abs, consequently vacuolar sorting should be considered as an alternative strategy to obtain high protein yields.Facultad de Ciencias ExactasHumana2018-06-19info:eu-repo/semantics/bookPartinfo:eu-repo/semantics/publishedVersionCapitulo de librohttp://purl.org/coar/resource_type/c_3248info:ar-repo/semantics/parteDeLibroapplication/pdf65-80http://sedici.unlp.edu.ar/handle/10915/138728enginfo:eu-repo/semantics/altIdentifier/isbn/978-1-4939-7856-4info:eu-repo/semantics/altIdentifier/issn/1940-6029info:eu-repo/semantics/altIdentifier/issn/1064-3745info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-7856-4_6info:eu-repo/semantics/altIdentifier/pmid/29916072info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:03:58Zoai:sedici.unlp.edu.ar:10915/138728Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:03:58.548SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Vacuolar Targeting and Characterization of Recombinant Antibodies
title Vacuolar Targeting and Characterization of Recombinant Antibodies
spellingShingle Vacuolar Targeting and Characterization of Recombinant Antibodies
Ocampo, Carolina Gabriela
Ciencias Exactas
Biología
Immunoglobulin
N-glycosylation
Vacuolar sorting signals
Secretory pathway
Vacuolar transport
Molecular farming
title_short Vacuolar Targeting and Characterization of Recombinant Antibodies
title_full Vacuolar Targeting and Characterization of Recombinant Antibodies
title_fullStr Vacuolar Targeting and Characterization of Recombinant Antibodies
title_full_unstemmed Vacuolar Targeting and Characterization of Recombinant Antibodies
title_sort Vacuolar Targeting and Characterization of Recombinant Antibodies
dc.creator.none.fl_str_mv Ocampo, Carolina Gabriela
Petruccelli, Silvana
Pereira, Cláudia
author Ocampo, Carolina Gabriela
author_facet Ocampo, Carolina Gabriela
Petruccelli, Silvana
Pereira, Cláudia
author_role author
author2 Petruccelli, Silvana
Pereira, Cláudia
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Immunoglobulin
N-glycosylation
Vacuolar sorting signals
Secretory pathway
Vacuolar transport
Molecular farming
topic Ciencias Exactas
Biología
Immunoglobulin
N-glycosylation
Vacuolar sorting signals
Secretory pathway
Vacuolar transport
Molecular farming
dc.description.none.fl_txt_mv Plant-based platforms are extensively use for the expression of recombinant proteins, including monoclonal antibodies (mAbs). Generally, immunoglobulins (Igs) are sorted to the apoplast, which is often afflicted with intense proteolysis. Here, we describe methods to transiently express mAbs sorted to central vacuole in Nicotiana benthamiana leaves and to characterize the obtained IgG. Central vacuole is an appropriate compartment for the efficient production of Abs, consequently vacuolar sorting should be considered as an alternative strategy to obtain high protein yields.
Facultad de Ciencias Exactas
description Plant-based platforms are extensively use for the expression of recombinant proteins, including monoclonal antibodies (mAbs). Generally, immunoglobulins (Igs) are sorted to the apoplast, which is often afflicted with intense proteolysis. Here, we describe methods to transiently express mAbs sorted to central vacuole in Nicotiana benthamiana leaves and to characterize the obtained IgG. Central vacuole is an appropriate compartment for the efficient production of Abs, consequently vacuolar sorting should be considered as an alternative strategy to obtain high protein yields.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-19
dc.type.none.fl_str_mv info:eu-repo/semantics/bookPart
info:eu-repo/semantics/publishedVersion
Capitulo de libro
http://purl.org/coar/resource_type/c_3248
info:ar-repo/semantics/parteDeLibro
format bookPart
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/138728
url http://sedici.unlp.edu.ar/handle/10915/138728
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/isbn/978-1-4939-7856-4
info:eu-repo/semantics/altIdentifier/issn/1940-6029
info:eu-repo/semantics/altIdentifier/issn/1064-3745
info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-7856-4_6
info:eu-repo/semantics/altIdentifier/pmid/29916072
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
65-80
dc.publisher.none.fl_str_mv Humana
publisher.none.fl_str_mv Humana
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1842260538096091136
score 13.13397

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