Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi

Autores
Delgado Cerezo, Magdalena; Sánchez Rodríguez, Clara; Escudero, Viviana; Miedes, Eva; Fernández, Paula Virginia; Jordá, Lucía; Hernández Blanco, Camilo; Sánchez Vallet, Andrea; Bednarek, Pawel; Schulze Lefert, Paul; Somerville, Shauna; Estevez, Jose Manuel; Persson, Staffan; Molina, Antonio
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gb subunit displays enhanced susceptibility to these pathogens. Gb/AGB1 forms an obligate dimer with either one of the Arabidopsis Gg subunits (g1/AGG1 and g2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulatedgenes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gband Gg1/g2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.
Fil: Delgado Cerezo, Magdalena. Universidad Politécnica de Madrid; España
Fil: Sánchez Rodríguez, Clara. Universidad Politécnica de Madrid; España. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Escudero, Viviana. Universidad Politécnica de Madrid; España
Fil: Miedes, Eva. Universidad Politécnica de Madrid; España
Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Jordá, Lucía. Universidad Politécnica de Madrid; España
Fil: Hernández Blanco, Camilo. Universidad Politécnica de Madrid; España
Fil: Sánchez Vallet, Andrea. Universidad Politécnica de Madrid; España. University of Agriculture Wageningen; Países Bajos
Fil: Bednarek, Pawel. Polish Academy of Sciences; Argentina. Max Planck Institut fur Zuchtungsforschung; Alemania
Fil: Schulze Lefert, Paul. Max Planck Institut fur Zuchtungsforschung; Alemania
Fil: Somerville, Shauna. University of California at Berkeley; Estados Unidos
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Persson, Staffan. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Molina, Antonio. Universidad Politécnica de Madrid; España
Materia
G-protein
Cell wall
Pathogen resistance
Arabidopsis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268613
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/268613
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic FungiDelgado Cerezo, MagdalenaSánchez Rodríguez, ClaraEscudero, VivianaMiedes, EvaFernández, Paula VirginiaJordá, LucíaHernández Blanco, CamiloSánchez Vallet, AndreaBednarek, PawelSchulze Lefert, PaulSomerville, ShaunaEstevez, Jose ManuelPersson, StaffanMolina, AntonioG-proteinCell wallPathogen resistanceArabidopsishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gb subunit displays enhanced susceptibility to these pathogens. Gb/AGB1 forms an obligate dimer with either one of the Arabidopsis Gg subunits (g1/AGG1 and g2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulatedgenes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gband Gg1/g2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.Fil: Delgado Cerezo, Magdalena. Universidad Politécnica de Madrid; EspañaFil: Sánchez Rodríguez, Clara. Universidad Politécnica de Madrid; España. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Escudero, Viviana. Universidad Politécnica de Madrid; EspañaFil: Miedes, Eva. Universidad Politécnica de Madrid; EspañaFil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Jordá, Lucía. Universidad Politécnica de Madrid; EspañaFil: Hernández Blanco, Camilo. Universidad Politécnica de Madrid; EspañaFil: Sánchez Vallet, Andrea. Universidad Politécnica de Madrid; España. University of Agriculture Wageningen; Países BajosFil: Bednarek, Pawel. Polish Academy of Sciences; Argentina. Max Planck Institut fur Zuchtungsforschung; AlemaniaFil: Schulze Lefert, Paul. Max Planck Institut fur Zuchtungsforschung; AlemaniaFil: Somerville, Shauna. University of California at Berkeley; Estados UnidosFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Persson, Staffan. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Molina, Antonio. Universidad Politécnica de Madrid; EspañaOxford University Press2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268613Delgado Cerezo, Magdalena; Sánchez Rodríguez, Clara; Escudero, Viviana; Miedes, Eva; Fernández, Paula Virginia; et al.; Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi; Oxford University Press; Molecular Plant; 5; 1; 1-2012; 98-1141674-2052CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S167420521460650Xinfo:eu-repo/semantics/altIdentifier/doi/10.1093/mp/ssr082info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:31:25Zoai:ri.conicet.gov.ar:11336/268613instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:31:25.969CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi
title Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi
spellingShingle Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi
Delgado Cerezo, Magdalena
G-protein
Cell wall
Pathogen resistance
Arabidopsis
title_short Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi
title_full Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi
title_fullStr Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi
title_full_unstemmed Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi
title_sort Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi
dc.creator.none.fl_str_mv Delgado Cerezo, Magdalena
Sánchez Rodríguez, Clara
Escudero, Viviana
Miedes, Eva
Fernández, Paula Virginia
Jordá, Lucía
Hernández Blanco, Camilo
Sánchez Vallet, Andrea
Bednarek, Pawel
Schulze Lefert, Paul
Somerville, Shauna
Estevez, Jose Manuel
Persson, Staffan
Molina, Antonio
author Delgado Cerezo, Magdalena
author_facet Delgado Cerezo, Magdalena
Sánchez Rodríguez, Clara
Escudero, Viviana
Miedes, Eva
Fernández, Paula Virginia
Jordá, Lucía
Hernández Blanco, Camilo
Sánchez Vallet, Andrea
Bednarek, Pawel
Schulze Lefert, Paul
Somerville, Shauna
Estevez, Jose Manuel
Persson, Staffan
Molina, Antonio
author_role author
author2 Sánchez Rodríguez, Clara
Escudero, Viviana
Miedes, Eva
Fernández, Paula Virginia
Jordá, Lucía
Hernández Blanco, Camilo
Sánchez Vallet, Andrea
Bednarek, Pawel
Schulze Lefert, Paul
Somerville, Shauna
Estevez, Jose Manuel
Persson, Staffan
Molina, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv G-protein
Cell wall
Pathogen resistance
Arabidopsis
topic G-protein
Cell wall
Pathogen resistance
Arabidopsis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gb subunit displays enhanced susceptibility to these pathogens. Gb/AGB1 forms an obligate dimer with either one of the Arabidopsis Gg subunits (g1/AGG1 and g2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulatedgenes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gband Gg1/g2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.
Fil: Delgado Cerezo, Magdalena. Universidad Politécnica de Madrid; España
Fil: Sánchez Rodríguez, Clara. Universidad Politécnica de Madrid; España. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Escudero, Viviana. Universidad Politécnica de Madrid; España
Fil: Miedes, Eva. Universidad Politécnica de Madrid; España
Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Jordá, Lucía. Universidad Politécnica de Madrid; España
Fil: Hernández Blanco, Camilo. Universidad Politécnica de Madrid; España
Fil: Sánchez Vallet, Andrea. Universidad Politécnica de Madrid; España. University of Agriculture Wageningen; Países Bajos
Fil: Bednarek, Pawel. Polish Academy of Sciences; Argentina. Max Planck Institut fur Zuchtungsforschung; Alemania
Fil: Schulze Lefert, Paul. Max Planck Institut fur Zuchtungsforschung; Alemania
Fil: Somerville, Shauna. University of California at Berkeley; Estados Unidos
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Persson, Staffan. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Molina, Antonio. Universidad Politécnica de Madrid; España
description The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gb subunit displays enhanced susceptibility to these pathogens. Gb/AGB1 forms an obligate dimer with either one of the Arabidopsis Gg subunits (g1/AGG1 and g2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulatedgenes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gband Gg1/g2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268613
Delgado Cerezo, Magdalena; Sánchez Rodríguez, Clara; Escudero, Viviana; Miedes, Eva; Fernández, Paula Virginia; et al.; Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi; Oxford University Press; Molecular Plant; 5; 1; 1-2012; 98-114
1674-2052
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268613
identifier_str_mv Delgado Cerezo, Magdalena; Sánchez Rodríguez, Clara; Escudero, Viviana; Miedes, Eva; Fernández, Paula Virginia; et al.; Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi; Oxford University Press; Molecular Plant; 5; 1; 1-2012; 98-114
1674-2052
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S167420521460650X
info:eu-repo/semantics/altIdentifier/doi/10.1093/mp/ssr082
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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