Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi
- Autores
- Delgado Cerezo, Magdalena; Sánchez Rodríguez, Clara; Escudero, Viviana; Miedes, Eva; Fernández, Paula Virginia; Jordá, Lucía; Hernández Blanco, Camilo; Sánchez Vallet, Andrea; Bednarek, Pawel; Schulze Lefert, Paul; Somerville, Shauna; Estevez, Jose Manuel; Persson, Staffan; Molina, Antonio
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gb subunit displays enhanced susceptibility to these pathogens. Gb/AGB1 forms an obligate dimer with either one of the Arabidopsis Gg subunits (g1/AGG1 and g2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulatedgenes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gband Gg1/g2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.
Fil: Delgado Cerezo, Magdalena. Universidad Politécnica de Madrid; España
Fil: Sánchez Rodríguez, Clara. Universidad Politécnica de Madrid; España. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Escudero, Viviana. Universidad Politécnica de Madrid; España
Fil: Miedes, Eva. Universidad Politécnica de Madrid; España
Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Jordá, Lucía. Universidad Politécnica de Madrid; España
Fil: Hernández Blanco, Camilo. Universidad Politécnica de Madrid; España
Fil: Sánchez Vallet, Andrea. Universidad Politécnica de Madrid; España. University of Agriculture Wageningen; Países Bajos
Fil: Bednarek, Pawel. Polish Academy of Sciences; Argentina. Max Planck Institut fur Zuchtungsforschung; Alemania
Fil: Schulze Lefert, Paul. Max Planck Institut fur Zuchtungsforschung; Alemania
Fil: Somerville, Shauna. University of California at Berkeley; Estados Unidos
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Persson, Staffan. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Molina, Antonio. Universidad Politécnica de Madrid; España - Materia
-
G-protein
Cell wall
Pathogen resistance
Arabidopsis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/268613
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Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic FungiDelgado Cerezo, MagdalenaSánchez Rodríguez, ClaraEscudero, VivianaMiedes, EvaFernández, Paula VirginiaJordá, LucíaHernández Blanco, CamiloSánchez Vallet, AndreaBednarek, PawelSchulze Lefert, PaulSomerville, ShaunaEstevez, Jose ManuelPersson, StaffanMolina, AntonioG-proteinCell wallPathogen resistanceArabidopsishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gb subunit displays enhanced susceptibility to these pathogens. Gb/AGB1 forms an obligate dimer with either one of the Arabidopsis Gg subunits (g1/AGG1 and g2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulatedgenes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gband Gg1/g2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.Fil: Delgado Cerezo, Magdalena. Universidad Politécnica de Madrid; EspañaFil: Sánchez Rodríguez, Clara. Universidad Politécnica de Madrid; España. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Escudero, Viviana. Universidad Politécnica de Madrid; EspañaFil: Miedes, Eva. Universidad Politécnica de Madrid; EspañaFil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Jordá, Lucía. Universidad Politécnica de Madrid; EspañaFil: Hernández Blanco, Camilo. Universidad Politécnica de Madrid; EspañaFil: Sánchez Vallet, Andrea. Universidad Politécnica de Madrid; España. University of Agriculture Wageningen; Países BajosFil: Bednarek, Pawel. Polish Academy of Sciences; Argentina. Max Planck Institut fur Zuchtungsforschung; AlemaniaFil: Schulze Lefert, Paul. Max Planck Institut fur Zuchtungsforschung; AlemaniaFil: Somerville, Shauna. University of California at Berkeley; Estados UnidosFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Persson, Staffan. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Molina, Antonio. Universidad Politécnica de Madrid; EspañaOxford University Press2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268613Delgado Cerezo, Magdalena; Sánchez Rodríguez, Clara; Escudero, Viviana; Miedes, Eva; Fernández, Paula Virginia; et al.; Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi; Oxford University Press; Molecular Plant; 5; 1; 1-2012; 98-1141674-2052CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S167420521460650Xinfo:eu-repo/semantics/altIdentifier/doi/10.1093/mp/ssr082info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:55Zoai:ri.conicet.gov.ar:11336/268613instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:55.415CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi |
title |
Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi |
spellingShingle |
Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi Delgado Cerezo, Magdalena G-protein Cell wall Pathogen resistance Arabidopsis |
title_short |
Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi |
title_full |
Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi |
title_fullStr |
Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi |
title_full_unstemmed |
Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi |
title_sort |
Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi |
dc.creator.none.fl_str_mv |
Delgado Cerezo, Magdalena Sánchez Rodríguez, Clara Escudero, Viviana Miedes, Eva Fernández, Paula Virginia Jordá, Lucía Hernández Blanco, Camilo Sánchez Vallet, Andrea Bednarek, Pawel Schulze Lefert, Paul Somerville, Shauna Estevez, Jose Manuel Persson, Staffan Molina, Antonio |
author |
Delgado Cerezo, Magdalena |
author_facet |
Delgado Cerezo, Magdalena Sánchez Rodríguez, Clara Escudero, Viviana Miedes, Eva Fernández, Paula Virginia Jordá, Lucía Hernández Blanco, Camilo Sánchez Vallet, Andrea Bednarek, Pawel Schulze Lefert, Paul Somerville, Shauna Estevez, Jose Manuel Persson, Staffan Molina, Antonio |
author_role |
author |
author2 |
Sánchez Rodríguez, Clara Escudero, Viviana Miedes, Eva Fernández, Paula Virginia Jordá, Lucía Hernández Blanco, Camilo Sánchez Vallet, Andrea Bednarek, Pawel Schulze Lefert, Paul Somerville, Shauna Estevez, Jose Manuel Persson, Staffan Molina, Antonio |
author2_role |
author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
G-protein Cell wall Pathogen resistance Arabidopsis |
topic |
G-protein Cell wall Pathogen resistance Arabidopsis |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gb subunit displays enhanced susceptibility to these pathogens. Gb/AGB1 forms an obligate dimer with either one of the Arabidopsis Gg subunits (g1/AGG1 and g2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulatedgenes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gband Gg1/g2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition. Fil: Delgado Cerezo, Magdalena. Universidad Politécnica de Madrid; España Fil: Sánchez Rodríguez, Clara. Universidad Politécnica de Madrid; España. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Escudero, Viviana. Universidad Politécnica de Madrid; España Fil: Miedes, Eva. Universidad Politécnica de Madrid; España Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Jordá, Lucía. Universidad Politécnica de Madrid; España Fil: Hernández Blanco, Camilo. Universidad Politécnica de Madrid; España Fil: Sánchez Vallet, Andrea. Universidad Politécnica de Madrid; España. University of Agriculture Wageningen; Países Bajos Fil: Bednarek, Pawel. Polish Academy of Sciences; Argentina. Max Planck Institut fur Zuchtungsforschung; Alemania Fil: Schulze Lefert, Paul. Max Planck Institut fur Zuchtungsforschung; Alemania Fil: Somerville, Shauna. University of California at Berkeley; Estados Unidos Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Persson, Staffan. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Molina, Antonio. Universidad Politécnica de Madrid; España |
description |
The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gb subunit displays enhanced susceptibility to these pathogens. Gb/AGB1 forms an obligate dimer with either one of the Arabidopsis Gg subunits (g1/AGG1 and g2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulatedgenes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gband Gg1/g2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/268613 Delgado Cerezo, Magdalena; Sánchez Rodríguez, Clara; Escudero, Viviana; Miedes, Eva; Fernández, Paula Virginia; et al.; Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi; Oxford University Press; Molecular Plant; 5; 1; 1-2012; 98-114 1674-2052 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/268613 |
identifier_str_mv |
Delgado Cerezo, Magdalena; Sánchez Rodríguez, Clara; Escudero, Viviana; Miedes, Eva; Fernández, Paula Virginia; et al.; Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi; Oxford University Press; Molecular Plant; 5; 1; 1-2012; 98-114 1674-2052 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S167420521460650X info:eu-repo/semantics/altIdentifier/doi/10.1093/mp/ssr082 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613818190135296 |
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13.070432 |