Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi

Autores
Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Berni, Elias; Camí, Gerardo Enrique; Durán, Nelson; Raba, Julio; Sanz Ferramola, Maria Isabel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study investigated the biological synthesis, characterization and antifungal activity of silver nanoparticles (Ag NPs). The production of Ag NPs was performed using the culture supernatants of two yeasts: Cryptococcus laurentii and Rhodotorula glutinis. These yeasts were chosen for their nitrate reductase activity. The role of nitrate reductase in the production of nanoparticles was assessed using inhibitors. The characterization of Ag NPs was made by UV-visible spectrophotometry, photon correlation spectroscopy, transmission electron microscopy and X-ray diffraction. Moreover, the FTIR spectrum identified the possible stabilizing agents present in supernatants. The Ag NPs obtained from both yeasts were disperse and stable and exhibited differences in sizes, zeta potential, concentration and stabilizing compounds. The antifungal activity of the Ag NPs was evaluated against the relevant phytopathogenic fungi, the common producers of postharvest diseases in pome fruits. Susceptibility tests on agar demonstrated that the antifungal activity of the nanoparticles from R. glutinis was higher than that from the ones from C. laurentii, and both were significantly more effective for inhibiting the fungi than the nanoparticles made from chemical synthesis. At 3 ppm, the nanoparticles from R. glutinis had similar efficacy to iprodione, a fungicide commonly utilized for combating postharvest diseases.
Fil: Fernández, Jorge Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Berni, Elias. Universidade Estadual de Campinas; Brasil
Fil: Camí, Gerardo Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Durán, Nelson. Universidade Estadual de Campinas; Brasil. Universidade Federal do ABC; Brasil
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
Antifungal Activity
Cryptococcus Laurentii
Postharvest Phytopathogen Fungi
Rhodotorula Glutinis
Silver Nanoparticles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60571
Acceder
id CONICETDig_8af0e7579706fe47298ed750cbce7a06
oai_identifier_str oai:ri.conicet.gov.ar:11336/60571
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungiFernández, Jorge GastónFernández Baldo, Martín AlejandroBerni, EliasCamí, Gerardo EnriqueDurán, NelsonRaba, JulioSanz Ferramola, Maria IsabelAntifungal ActivityCryptococcus LaurentiiPostharvest Phytopathogen FungiRhodotorula GlutinisSilver Nanoparticleshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present study investigated the biological synthesis, characterization and antifungal activity of silver nanoparticles (Ag NPs). The production of Ag NPs was performed using the culture supernatants of two yeasts: Cryptococcus laurentii and Rhodotorula glutinis. These yeasts were chosen for their nitrate reductase activity. The role of nitrate reductase in the production of nanoparticles was assessed using inhibitors. The characterization of Ag NPs was made by UV-visible spectrophotometry, photon correlation spectroscopy, transmission electron microscopy and X-ray diffraction. Moreover, the FTIR spectrum identified the possible stabilizing agents present in supernatants. The Ag NPs obtained from both yeasts were disperse and stable and exhibited differences in sizes, zeta potential, concentration and stabilizing compounds. The antifungal activity of the Ag NPs was evaluated against the relevant phytopathogenic fungi, the common producers of postharvest diseases in pome fruits. Susceptibility tests on agar demonstrated that the antifungal activity of the nanoparticles from R. glutinis was higher than that from the ones from C. laurentii, and both were significantly more effective for inhibiting the fungi than the nanoparticles made from chemical synthesis. At 3 ppm, the nanoparticles from R. glutinis had similar efficacy to iprodione, a fungicide commonly utilized for combating postharvest diseases.Fil: Fernández, Jorge Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Berni, Elias. Universidade Estadual de Campinas; BrasilFil: Camí, Gerardo Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Durán, Nelson. Universidade Estadual de Campinas; Brasil. Universidade Federal do ABC; BrasilFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Sanz Ferramola, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60571Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Berni, Elias; Camí, Gerardo Enrique; Durán, Nelson; et al.; Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi; Elsevier; Process Biochemistry; 51; 9; 9-2016; 1306-13131359-5113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511316301441info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2016.05.021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:42:00Zoai:ri.conicet.gov.ar:11336/60571instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:42:01.055CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi
title Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi
spellingShingle Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi
Fernández, Jorge Gastón
Antifungal Activity
Cryptococcus Laurentii
Postharvest Phytopathogen Fungi
Rhodotorula Glutinis
Silver Nanoparticles
title_short Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi
title_full Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi
title_fullStr Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi
title_full_unstemmed Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi
title_sort Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi
dc.creator.none.fl_str_mv Fernández, Jorge Gastón
Fernández Baldo, Martín Alejandro
Berni, Elias
Camí, Gerardo Enrique
Durán, Nelson
Raba, Julio
Sanz Ferramola, Maria Isabel
author Fernández, Jorge Gastón
author_facet Fernández, Jorge Gastón
Fernández Baldo, Martín Alejandro
Berni, Elias
Camí, Gerardo Enrique
Durán, Nelson
Raba, Julio
Sanz Ferramola, Maria Isabel
author_role author
author2 Fernández Baldo, Martín Alejandro
Berni, Elias
Camí, Gerardo Enrique
Durán, Nelson
Raba, Julio
Sanz Ferramola, Maria Isabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Antifungal Activity
Cryptococcus Laurentii
Postharvest Phytopathogen Fungi
Rhodotorula Glutinis
Silver Nanoparticles
topic Antifungal Activity
Cryptococcus Laurentii
Postharvest Phytopathogen Fungi
Rhodotorula Glutinis
Silver Nanoparticles
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present study investigated the biological synthesis, characterization and antifungal activity of silver nanoparticles (Ag NPs). The production of Ag NPs was performed using the culture supernatants of two yeasts: Cryptococcus laurentii and Rhodotorula glutinis. These yeasts were chosen for their nitrate reductase activity. The role of nitrate reductase in the production of nanoparticles was assessed using inhibitors. The characterization of Ag NPs was made by UV-visible spectrophotometry, photon correlation spectroscopy, transmission electron microscopy and X-ray diffraction. Moreover, the FTIR spectrum identified the possible stabilizing agents present in supernatants. The Ag NPs obtained from both yeasts were disperse and stable and exhibited differences in sizes, zeta potential, concentration and stabilizing compounds. The antifungal activity of the Ag NPs was evaluated against the relevant phytopathogenic fungi, the common producers of postharvest diseases in pome fruits. Susceptibility tests on agar demonstrated that the antifungal activity of the nanoparticles from R. glutinis was higher than that from the ones from C. laurentii, and both were significantly more effective for inhibiting the fungi than the nanoparticles made from chemical synthesis. At 3 ppm, the nanoparticles from R. glutinis had similar efficacy to iprodione, a fungicide commonly utilized for combating postharvest diseases.
Fil: Fernández, Jorge Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Berni, Elias. Universidade Estadual de Campinas; Brasil
Fil: Camí, Gerardo Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Durán, Nelson. Universidade Estadual de Campinas; Brasil. Universidade Federal do ABC; Brasil
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description The present study investigated the biological synthesis, characterization and antifungal activity of silver nanoparticles (Ag NPs). The production of Ag NPs was performed using the culture supernatants of two yeasts: Cryptococcus laurentii and Rhodotorula glutinis. These yeasts were chosen for their nitrate reductase activity. The role of nitrate reductase in the production of nanoparticles was assessed using inhibitors. The characterization of Ag NPs was made by UV-visible spectrophotometry, photon correlation spectroscopy, transmission electron microscopy and X-ray diffraction. Moreover, the FTIR spectrum identified the possible stabilizing agents present in supernatants. The Ag NPs obtained from both yeasts were disperse and stable and exhibited differences in sizes, zeta potential, concentration and stabilizing compounds. The antifungal activity of the Ag NPs was evaluated against the relevant phytopathogenic fungi, the common producers of postharvest diseases in pome fruits. Susceptibility tests on agar demonstrated that the antifungal activity of the nanoparticles from R. glutinis was higher than that from the ones from C. laurentii, and both were significantly more effective for inhibiting the fungi than the nanoparticles made from chemical synthesis. At 3 ppm, the nanoparticles from R. glutinis had similar efficacy to iprodione, a fungicide commonly utilized for combating postharvest diseases.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60571
Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Berni, Elias; Camí, Gerardo Enrique; Durán, Nelson; et al.; Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi; Elsevier; Process Biochemistry; 51; 9; 9-2016; 1306-1313
1359-5113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60571
identifier_str_mv Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Berni, Elias; Camí, Gerardo Enrique; Durán, Nelson; et al.; Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi; Elsevier; Process Biochemistry; 51; 9; 9-2016; 1306-1313
1359-5113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511316301441
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2016.05.021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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