Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection

Autores
Marini, Vanina Natalia; Laiolo, Jerónimo; Lozano, Natalia; Varengo, Haydee Teresita
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Toxocara canis (T. canis) is an intestinal nematode that affects dogs. In humans, this geohelminth induces visceral larva migrans (VLM) syndrome, which is associated with eosinophilia, increased serum IgE and inflammation of the airways. In general, chronic nematode infections induce a polarized TH2 immune response.There are observations that the macrophages response to gastrointestinal nematodes in which the alternative activation pathway plays a more important role in resistance. However, whether or not Toxocara infectionsinduce alternative activation of human monocytes/macrophages is still a matter of debate. The objective of this study was to determine the pattern of IL12 and IL10 levels and nitric oxide (NO) production in vitro of human adherent cells against antigens from T. canis. For this purpose the in vitro cytokine production of adherent cells from healthy subjects was examined in response to stimulation with antigens from larval stage of T. canis.Cell culture supernatants were removed from antigen stimulated and unstimulated cultures after 24 hours, 15 and 20 days post infection and the concentration of IL-12, IL-10 and NO concentrations were determined.The results revealed high levels of IL-12 at 24 hours post stimulation. After that time, the concentration of this cytoquine declined to undetectable levels. IL-10 revealed a progressive increase from the 24 hours until the 20th day post stimulation. Conversely not detectable levels of NO were found during the study. Therefore,we concluded that during early T. canis infection, the activation and suppression of immune processes occur simultaneously and cytokines of adherent cells contribute to the regulation of the immune response.
Fil: Marini, Vanina Natalia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Laiolo, Jerónimo. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lozano, Natalia. Clinica Universitaria Reina Fabiola; Argentina
Fil: Varengo, Haydee Teresita. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Materia
TOXOCARA CANIS
NITRIC OXIDE
INTERLEUKIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/108239
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infectionMarini, Vanina NataliaLaiolo, JerónimoLozano, NataliaVarengo, Haydee TeresitaTOXOCARA CANISNITRIC OXIDEINTERLEUKINhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Toxocara canis (T. canis) is an intestinal nematode that affects dogs. In humans, this geohelminth induces visceral larva migrans (VLM) syndrome, which is associated with eosinophilia, increased serum IgE and inflammation of the airways. In general, chronic nematode infections induce a polarized TH2 immune response.There are observations that the macrophages response to gastrointestinal nematodes in which the alternative activation pathway plays a more important role in resistance. However, whether or not Toxocara infectionsinduce alternative activation of human monocytes/macrophages is still a matter of debate. The objective of this study was to determine the pattern of IL12 and IL10 levels and nitric oxide (NO) production in vitro of human adherent cells against antigens from T. canis. For this purpose the in vitro cytokine production of adherent cells from healthy subjects was examined in response to stimulation with antigens from larval stage of T. canis.Cell culture supernatants were removed from antigen stimulated and unstimulated cultures after 24 hours, 15 and 20 days post infection and the concentration of IL-12, IL-10 and NO concentrations were determined.The results revealed high levels of IL-12 at 24 hours post stimulation. After that time, the concentration of this cytoquine declined to undetectable levels. IL-10 revealed a progressive increase from the 24 hours until the 20th day post stimulation. Conversely not detectable levels of NO were found during the study. Therefore,we concluded that during early T. canis infection, the activation and suppression of immune processes occur simultaneously and cytokines of adherent cells contribute to the regulation of the immune response.Fil: Marini, Vanina Natalia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Laiolo, Jerónimo. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lozano, Natalia. Clinica Universitaria Reina Fabiola; ArgentinaFil: Varengo, Haydee Teresita. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaSociedad Chilena de Parasitología2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/108239Marini, Vanina Natalia; Laiolo, Jerónimo; Lozano, Natalia; Varengo, Haydee Teresita; Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection; Sociedad Chilena de Parasitología; Parasitologia Latinoamerica; 65; 3; 12-2016; 50-550719-6326CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sociedadchilenaparasitologia.cl/revista-parasitologia-latinoamericana/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:08Zoai:ri.conicet.gov.ar:11336/108239instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:08.716CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection
title Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection
spellingShingle Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection
Marini, Vanina Natalia
TOXOCARA CANIS
NITRIC OXIDE
INTERLEUKIN
title_short Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection
title_full Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection
title_fullStr Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection
title_full_unstemmed Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection
title_sort Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection
dc.creator.none.fl_str_mv Marini, Vanina Natalia
Laiolo, Jerónimo
Lozano, Natalia
Varengo, Haydee Teresita
author Marini, Vanina Natalia
author_facet Marini, Vanina Natalia
Laiolo, Jerónimo
Lozano, Natalia
Varengo, Haydee Teresita
author_role author
author2 Laiolo, Jerónimo
Lozano, Natalia
Varengo, Haydee Teresita
author2_role author
author
author
dc.subject.none.fl_str_mv TOXOCARA CANIS
NITRIC OXIDE
INTERLEUKIN
topic TOXOCARA CANIS
NITRIC OXIDE
INTERLEUKIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Toxocara canis (T. canis) is an intestinal nematode that affects dogs. In humans, this geohelminth induces visceral larva migrans (VLM) syndrome, which is associated with eosinophilia, increased serum IgE and inflammation of the airways. In general, chronic nematode infections induce a polarized TH2 immune response.There are observations that the macrophages response to gastrointestinal nematodes in which the alternative activation pathway plays a more important role in resistance. However, whether or not Toxocara infectionsinduce alternative activation of human monocytes/macrophages is still a matter of debate. The objective of this study was to determine the pattern of IL12 and IL10 levels and nitric oxide (NO) production in vitro of human adherent cells against antigens from T. canis. For this purpose the in vitro cytokine production of adherent cells from healthy subjects was examined in response to stimulation with antigens from larval stage of T. canis.Cell culture supernatants were removed from antigen stimulated and unstimulated cultures after 24 hours, 15 and 20 days post infection and the concentration of IL-12, IL-10 and NO concentrations were determined.The results revealed high levels of IL-12 at 24 hours post stimulation. After that time, the concentration of this cytoquine declined to undetectable levels. IL-10 revealed a progressive increase from the 24 hours until the 20th day post stimulation. Conversely not detectable levels of NO were found during the study. Therefore,we concluded that during early T. canis infection, the activation and suppression of immune processes occur simultaneously and cytokines of adherent cells contribute to the regulation of the immune response.
Fil: Marini, Vanina Natalia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Laiolo, Jerónimo. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lozano, Natalia. Clinica Universitaria Reina Fabiola; Argentina
Fil: Varengo, Haydee Teresita. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
description Toxocara canis (T. canis) is an intestinal nematode that affects dogs. In humans, this geohelminth induces visceral larva migrans (VLM) syndrome, which is associated with eosinophilia, increased serum IgE and inflammation of the airways. In general, chronic nematode infections induce a polarized TH2 immune response.There are observations that the macrophages response to gastrointestinal nematodes in which the alternative activation pathway plays a more important role in resistance. However, whether or not Toxocara infectionsinduce alternative activation of human monocytes/macrophages is still a matter of debate. The objective of this study was to determine the pattern of IL12 and IL10 levels and nitric oxide (NO) production in vitro of human adherent cells against antigens from T. canis. For this purpose the in vitro cytokine production of adherent cells from healthy subjects was examined in response to stimulation with antigens from larval stage of T. canis.Cell culture supernatants were removed from antigen stimulated and unstimulated cultures after 24 hours, 15 and 20 days post infection and the concentration of IL-12, IL-10 and NO concentrations were determined.The results revealed high levels of IL-12 at 24 hours post stimulation. After that time, the concentration of this cytoquine declined to undetectable levels. IL-10 revealed a progressive increase from the 24 hours until the 20th day post stimulation. Conversely not detectable levels of NO were found during the study. Therefore,we concluded that during early T. canis infection, the activation and suppression of immune processes occur simultaneously and cytokines of adherent cells contribute to the regulation of the immune response.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/108239
Marini, Vanina Natalia; Laiolo, Jerónimo; Lozano, Natalia; Varengo, Haydee Teresita; Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection; Sociedad Chilena de Parasitología; Parasitologia Latinoamerica; 65; 3; 12-2016; 50-55
0719-6326
CONICET Digital
CONICET
url http://hdl.handle.net/11336/108239
identifier_str_mv Marini, Vanina Natalia; Laiolo, Jerónimo; Lozano, Natalia; Varengo, Haydee Teresita; Human toxocariosis: in vitro production of soluble mediators against Toxocara canis infection; Sociedad Chilena de Parasitología; Parasitologia Latinoamerica; 65; 3; 12-2016; 50-55
0719-6326
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sociedadchilenaparasitologia.cl/revista-parasitologia-latinoamericana/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Chilena de Parasitología
publisher.none.fl_str_mv Sociedad Chilena de Parasitología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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