Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections

Autores
Leccese Terraf, Maria Cecilia; Juárez Tomás, María Silvina; Mendoza, Lucia Margarita; Silva, Clara del Valle; Nader, Maria Elena Fatima
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Lactobacilli are the dominant microorganisms of the healthy human vagina and participate in the maintenance of the ecological balance of the urogenital tract. They can inhibit the growth of urogenital pathogens by different mechanisms. The intravaginal administration of beneficial lactobacilli in pharmaceutical products has been proposed to restore the vaginal microbiome. The biofilm formation of lactobacilli is a beneficial characteristic to favor their mucosal colonization and/or permanence. In this study, the virulence factors of clinical Escherichia coli isolates and the inhibitory ability of biofilm-forming vaginal lactobacilli against this uropathogen were evaluated. The resistance of E. coli 36 and 36a (isolated from a patient with pyelonephritis) and E. coli 275 (isolated from a patient with recurrent cystitis) to different antibiotics (determined by disc diffusion method, according to Clinical and Laboratory Clinical Institute recommendations) and the presence of virulence genes (by multiplex PCR) were assayed. Antimicrobial activity of vaginal Lactobacillus rhamnosus CRL1332, Lactobacillus reuteri CRL1324 and Lactobacillus gasseri CRL 1263 was assayed using the agar plate diffusion technique. Lactobacillus supernatant aliquots were assayed, untreated, neutralized with NaOH or neutralized and treated with catalase. E. coli 36 and 36a were susceptible to all the antibiotics tested, while E. coli 275 resulted an ESBL (extended-spectrum-beta-lactamase)-producing strain. In E. coli 36 and 36a, fimH, papAH (adhesins), fyuA (siderophore), traT (serum resistance) and kpsMTII (capsule) genes were detected. In E. coli 275, fimH, fyuA, traT and agn43 (biofilm formation) genes were evidenced. L. reuteri CRL1324 and L. rhamnosus CRL1332 were able to inhibit the growth of the three E. coli strains. The inhibitory activity against E. coli disappeared after the supernatant neutralization, indicating that organic acids are responsible of the antagonism. The Lactobacillus strains evaluated are promising probiotic candidates to prevent or treat urinary infections caused by E. coli.
Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Mendoza, Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Silva, Clara del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
3rd International Meeting on Pharmaceutical Sciences
Córdoba
Argentina
Universidad Nacional de Córdoba. Facultad de Ciencias Químicas
Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
Materia
VAGINAL LACTOBACILLI
UROPATHOGEN ESCHERICHIA COLI
ORGANIC ACID
PROBIOTICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263345
Acceder
id CONICETDig_8a835f432da1139621895aa2b20b2246
oai_identifier_str oai:ri.conicet.gov.ar:11336/263345
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infectionsLeccese Terraf, Maria CeciliaJuárez Tomás, María SilvinaMendoza, Lucia MargaritaSilva, Clara del ValleNader, Maria Elena FatimaVAGINAL LACTOBACILLIUROPATHOGEN ESCHERICHIA COLIORGANIC ACIDPROBIOTICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactobacilli are the dominant microorganisms of the healthy human vagina and participate in the maintenance of the ecological balance of the urogenital tract. They can inhibit the growth of urogenital pathogens by different mechanisms. The intravaginal administration of beneficial lactobacilli in pharmaceutical products has been proposed to restore the vaginal microbiome. The biofilm formation of lactobacilli is a beneficial characteristic to favor their mucosal colonization and/or permanence. In this study, the virulence factors of clinical Escherichia coli isolates and the inhibitory ability of biofilm-forming vaginal lactobacilli against this uropathogen were evaluated. The resistance of E. coli 36 and 36a (isolated from a patient with pyelonephritis) and E. coli 275 (isolated from a patient with recurrent cystitis) to different antibiotics (determined by disc diffusion method, according to Clinical and Laboratory Clinical Institute recommendations) and the presence of virulence genes (by multiplex PCR) were assayed. Antimicrobial activity of vaginal Lactobacillus rhamnosus CRL1332, Lactobacillus reuteri CRL1324 and Lactobacillus gasseri CRL 1263 was assayed using the agar plate diffusion technique. Lactobacillus supernatant aliquots were assayed, untreated, neutralized with NaOH or neutralized and treated with catalase. E. coli 36 and 36a were susceptible to all the antibiotics tested, while E. coli 275 resulted an ESBL (extended-spectrum-beta-lactamase)-producing strain. In E. coli 36 and 36a, fimH, papAH (adhesins), fyuA (siderophore), traT (serum resistance) and kpsMTII (capsule) genes were detected. In E. coli 275, fimH, fyuA, traT and agn43 (biofilm formation) genes were evidenced. L. reuteri CRL1324 and L. rhamnosus CRL1332 were able to inhibit the growth of the three E. coli strains. The inhibitory activity against E. coli disappeared after the supernatant neutralization, indicating that organic acids are responsible of the antagonism. The Lactobacillus strains evaluated are promising probiotic candidates to prevent or treat urinary infections caused by E. coli.Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Mendoza, Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Silva, Clara del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina3rd International Meeting on Pharmaceutical SciencesCórdobaArgentinaUniversidad Nacional de Córdoba. Facultad de Ciencias QuímicasUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y FarmacéuticasSociety of Pharmaceutical Sciences and Research2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263345Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections; 3rd International Meeting on Pharmaceutical Sciences; Córdoba; Argentina; 2014; 145-1462320-51480975-8232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ijpsr.com/conference-proceeding/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:17Zoai:ri.conicet.gov.ar:11336/263345instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:17.609CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections
title Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections
spellingShingle Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections
Leccese Terraf, Maria Cecilia
VAGINAL LACTOBACILLI
UROPATHOGEN ESCHERICHIA COLI
ORGANIC ACID
PROBIOTICS
title_short Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections
title_full Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections
title_fullStr Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections
title_full_unstemmed Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections
title_sort Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections
dc.creator.none.fl_str_mv Leccese Terraf, Maria Cecilia
Juárez Tomás, María Silvina
Mendoza, Lucia Margarita
Silva, Clara del Valle
Nader, Maria Elena Fatima
author Leccese Terraf, Maria Cecilia
author_facet Leccese Terraf, Maria Cecilia
Juárez Tomás, María Silvina
Mendoza, Lucia Margarita
Silva, Clara del Valle
Nader, Maria Elena Fatima
author_role author
author2 Juárez Tomás, María Silvina
Mendoza, Lucia Margarita
Silva, Clara del Valle
Nader, Maria Elena Fatima
author2_role author
author
author
author
dc.subject.none.fl_str_mv VAGINAL LACTOBACILLI
UROPATHOGEN ESCHERICHIA COLI
ORGANIC ACID
PROBIOTICS
topic VAGINAL LACTOBACILLI
UROPATHOGEN ESCHERICHIA COLI
ORGANIC ACID
PROBIOTICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactobacilli are the dominant microorganisms of the healthy human vagina and participate in the maintenance of the ecological balance of the urogenital tract. They can inhibit the growth of urogenital pathogens by different mechanisms. The intravaginal administration of beneficial lactobacilli in pharmaceutical products has been proposed to restore the vaginal microbiome. The biofilm formation of lactobacilli is a beneficial characteristic to favor their mucosal colonization and/or permanence. In this study, the virulence factors of clinical Escherichia coli isolates and the inhibitory ability of biofilm-forming vaginal lactobacilli against this uropathogen were evaluated. The resistance of E. coli 36 and 36a (isolated from a patient with pyelonephritis) and E. coli 275 (isolated from a patient with recurrent cystitis) to different antibiotics (determined by disc diffusion method, according to Clinical and Laboratory Clinical Institute recommendations) and the presence of virulence genes (by multiplex PCR) were assayed. Antimicrobial activity of vaginal Lactobacillus rhamnosus CRL1332, Lactobacillus reuteri CRL1324 and Lactobacillus gasseri CRL 1263 was assayed using the agar plate diffusion technique. Lactobacillus supernatant aliquots were assayed, untreated, neutralized with NaOH or neutralized and treated with catalase. E. coli 36 and 36a were susceptible to all the antibiotics tested, while E. coli 275 resulted an ESBL (extended-spectrum-beta-lactamase)-producing strain. In E. coli 36 and 36a, fimH, papAH (adhesins), fyuA (siderophore), traT (serum resistance) and kpsMTII (capsule) genes were detected. In E. coli 275, fimH, fyuA, traT and agn43 (biofilm formation) genes were evidenced. L. reuteri CRL1324 and L. rhamnosus CRL1332 were able to inhibit the growth of the three E. coli strains. The inhibitory activity against E. coli disappeared after the supernatant neutralization, indicating that organic acids are responsible of the antagonism. The Lactobacillus strains evaluated are promising probiotic candidates to prevent or treat urinary infections caused by E. coli.
Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Mendoza, Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Silva, Clara del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
3rd International Meeting on Pharmaceutical Sciences
Córdoba
Argentina
Universidad Nacional de Córdoba. Facultad de Ciencias Químicas
Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
description Lactobacilli are the dominant microorganisms of the healthy human vagina and participate in the maintenance of the ecological balance of the urogenital tract. They can inhibit the growth of urogenital pathogens by different mechanisms. The intravaginal administration of beneficial lactobacilli in pharmaceutical products has been proposed to restore the vaginal microbiome. The biofilm formation of lactobacilli is a beneficial characteristic to favor their mucosal colonization and/or permanence. In this study, the virulence factors of clinical Escherichia coli isolates and the inhibitory ability of biofilm-forming vaginal lactobacilli against this uropathogen were evaluated. The resistance of E. coli 36 and 36a (isolated from a patient with pyelonephritis) and E. coli 275 (isolated from a patient with recurrent cystitis) to different antibiotics (determined by disc diffusion method, according to Clinical and Laboratory Clinical Institute recommendations) and the presence of virulence genes (by multiplex PCR) were assayed. Antimicrobial activity of vaginal Lactobacillus rhamnosus CRL1332, Lactobacillus reuteri CRL1324 and Lactobacillus gasseri CRL 1263 was assayed using the agar plate diffusion technique. Lactobacillus supernatant aliquots were assayed, untreated, neutralized with NaOH or neutralized and treated with catalase. E. coli 36 and 36a were susceptible to all the antibiotics tested, while E. coli 275 resulted an ESBL (extended-spectrum-beta-lactamase)-producing strain. In E. coli 36 and 36a, fimH, papAH (adhesins), fyuA (siderophore), traT (serum resistance) and kpsMTII (capsule) genes were detected. In E. coli 275, fimH, fyuA, traT and agn43 (biofilm formation) genes were evidenced. L. reuteri CRL1324 and L. rhamnosus CRL1332 were able to inhibit the growth of the three E. coli strains. The inhibitory activity against E. coli disappeared after the supernatant neutralization, indicating that organic acids are responsible of the antagonism. The Lactobacillus strains evaluated are promising probiotic candidates to prevent or treat urinary infections caused by E. coli.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263345
Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections; 3rd International Meeting on Pharmaceutical Sciences; Córdoba; Argentina; 2014; 145-146
2320-5148
0975-8232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263345
identifier_str_mv Biofilm-forming vaginal lactobacilli inhibit Escherichia coli isolated from women with urinary infections; 3rd International Meeting on Pharmaceutical Sciences; Córdoba; Argentina; 2014; 145-146
2320-5148
0975-8232
CONICET Digital
CONICET
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Society of Pharmaceutical Sciences and Research
publisher.none.fl_str_mv Society of Pharmaceutical Sciences and Research
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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