Use of fungal laccases for aflatoxin reduction in maize sub-products
- Autores
- Bossa, Marianela; Monesterolo, Noelia Edith; Monge, Maria del Pilar; Palazzini, Juan Manuel; Pena, Gabriela Alejandra; Chulza, Sofia; Alaniz Zanon, Maria Silvina; Chiotta, María Laura
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Fungal contamination is a phytosanitary problem of concern in maize and by-products. Aspergillus section Flavi strains can generate important problems due to contamination with aflatoxins (AFs). During the bioethanol production from maize, AFs levels can increase up to three times in the final co-product (DDGS or WDGS), intended for animal feed. Mosto is the milled maize grains with the addition of water and amylase enzymes that enter the fermentation stage. One strategy to reduce the mycotoxin contamination levels is the use of microorganisms or enzymes able to metabolize, destroy or inactivate those compounds. Laccases are enzymes with biocatalytic capacity and wide biotechnological application, including food industry. Particularly, laccases from fungi that cause white rot have been proposed for mycotoxin biotransformation. In the present work, the effectiveness of different fungal laccases in reducing AFs levels (AFB1, AFG1, AFB2 and AFG2) was evaluated under an in vitro assay. Laccase production by Phylum Basidiomycota strains was evaluated and 9 of them were selected to carry out in vitro decontamination tests. A concentration of 1 µg/mL of AFB1 and AFG1, and 0.25 µg/mL of AFB2 and AFG2 were used at concentrations of 5, 10, 15 and 20 U/mL of laccases contained in enzyme extracts, in addition to vanillic acid as redox mediator (1 and 10 mM). The strain Trametes sp. B7-IMICO-RC was able to degrade the 4 AFs at high levels under all the evaluated conditions. The highest degradation percentage was reached in presence of 1 mM vanillic acid and 20 U/mL laccase (88, 99, 87 and 70%, for AFB1, AFG1, AFB2 and AFG2, respectively). An additional in vitro degradation assay of AFs was carried out using mosto as the substrate. The degradation percentages observed under these same conditions were 26% for AFB1 and AFG2 and 54% for AFG1. Currently, studies are being carried out in order to determine the degradation products toxicity. These results would contribute to the development of an eco-friendly strategy to reduce mycotoxin contamination, ensuring food safety.
Fil: Bossa, Marianela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Monesterolo, Noelia Edith. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Monge, Maria del Pilar. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Palazzini, Juan Manuel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Pena, Gabriela Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Chulza, Sofia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Alaniz Zanon, Maria Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
14th conference of the World Mycotoxin Forum
Amberes
Bélgica
Committee Organisation of 14th conference of the World Mycotoxin Forum - Materia
-
BASIDIOMYCETES
LACCASES
AFLATOXINS
DDGS O WDGS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/252003
Ver los metadatos del registro completo
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Use of fungal laccases for aflatoxin reduction in maize sub-productsBossa, MarianelaMonesterolo, Noelia EdithMonge, Maria del PilarPalazzini, Juan ManuelPena, Gabriela AlejandraChulza, SofiaAlaniz Zanon, Maria SilvinaChiotta, María LauraBASIDIOMYCETESLACCASESAFLATOXINSDDGS O WDGShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fungal contamination is a phytosanitary problem of concern in maize and by-products. Aspergillus section Flavi strains can generate important problems due to contamination with aflatoxins (AFs). During the bioethanol production from maize, AFs levels can increase up to three times in the final co-product (DDGS or WDGS), intended for animal feed. Mosto is the milled maize grains with the addition of water and amylase enzymes that enter the fermentation stage. One strategy to reduce the mycotoxin contamination levels is the use of microorganisms or enzymes able to metabolize, destroy or inactivate those compounds. Laccases are enzymes with biocatalytic capacity and wide biotechnological application, including food industry. Particularly, laccases from fungi that cause white rot have been proposed for mycotoxin biotransformation. In the present work, the effectiveness of different fungal laccases in reducing AFs levels (AFB1, AFG1, AFB2 and AFG2) was evaluated under an in vitro assay. Laccase production by Phylum Basidiomycota strains was evaluated and 9 of them were selected to carry out in vitro decontamination tests. A concentration of 1 µg/mL of AFB1 and AFG1, and 0.25 µg/mL of AFB2 and AFG2 were used at concentrations of 5, 10, 15 and 20 U/mL of laccases contained in enzyme extracts, in addition to vanillic acid as redox mediator (1 and 10 mM). The strain Trametes sp. B7-IMICO-RC was able to degrade the 4 AFs at high levels under all the evaluated conditions. The highest degradation percentage was reached in presence of 1 mM vanillic acid and 20 U/mL laccase (88, 99, 87 and 70%, for AFB1, AFG1, AFB2 and AFG2, respectively). An additional in vitro degradation assay of AFs was carried out using mosto as the substrate. The degradation percentages observed under these same conditions were 26% for AFB1 and AFG2 and 54% for AFG1. Currently, studies are being carried out in order to determine the degradation products toxicity. These results would contribute to the development of an eco-friendly strategy to reduce mycotoxin contamination, ensuring food safety.Fil: Bossa, Marianela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Monesterolo, Noelia Edith. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Monge, Maria del Pilar. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Palazzini, Juan Manuel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Pena, Gabriela Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Chulza, Sofia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Alaniz Zanon, Maria Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina14th conference of the World Mycotoxin ForumAmberesBélgicaCommittee Organisation of 14th conference of the World Mycotoxin ForumCommittee Organisation of 14th conference of the World Mycotoxin Forum2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/252003Use of fungal laccases for aflatoxin reduction in maize sub-products; 14th conference of the World Mycotoxin Forum; Amberes; Bélgica; 2023; 128-128CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:48Zoai:ri.conicet.gov.ar:11336/252003instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:49.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Use of fungal laccases for aflatoxin reduction in maize sub-products |
| title |
Use of fungal laccases for aflatoxin reduction in maize sub-products |
| spellingShingle |
Use of fungal laccases for aflatoxin reduction in maize sub-products Bossa, Marianela BASIDIOMYCETES LACCASES AFLATOXINS DDGS O WDGS |
| title_short |
Use of fungal laccases for aflatoxin reduction in maize sub-products |
| title_full |
Use of fungal laccases for aflatoxin reduction in maize sub-products |
| title_fullStr |
Use of fungal laccases for aflatoxin reduction in maize sub-products |
| title_full_unstemmed |
Use of fungal laccases for aflatoxin reduction in maize sub-products |
| title_sort |
Use of fungal laccases for aflatoxin reduction in maize sub-products |
| dc.creator.none.fl_str_mv |
Bossa, Marianela Monesterolo, Noelia Edith Monge, Maria del Pilar Palazzini, Juan Manuel Pena, Gabriela Alejandra Chulza, Sofia Alaniz Zanon, Maria Silvina Chiotta, María Laura |
| author |
Bossa, Marianela |
| author_facet |
Bossa, Marianela Monesterolo, Noelia Edith Monge, Maria del Pilar Palazzini, Juan Manuel Pena, Gabriela Alejandra Chulza, Sofia Alaniz Zanon, Maria Silvina Chiotta, María Laura |
| author_role |
author |
| author2 |
Monesterolo, Noelia Edith Monge, Maria del Pilar Palazzini, Juan Manuel Pena, Gabriela Alejandra Chulza, Sofia Alaniz Zanon, Maria Silvina Chiotta, María Laura |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
BASIDIOMYCETES LACCASES AFLATOXINS DDGS O WDGS |
| topic |
BASIDIOMYCETES LACCASES AFLATOXINS DDGS O WDGS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Fungal contamination is a phytosanitary problem of concern in maize and by-products. Aspergillus section Flavi strains can generate important problems due to contamination with aflatoxins (AFs). During the bioethanol production from maize, AFs levels can increase up to three times in the final co-product (DDGS or WDGS), intended for animal feed. Mosto is the milled maize grains with the addition of water and amylase enzymes that enter the fermentation stage. One strategy to reduce the mycotoxin contamination levels is the use of microorganisms or enzymes able to metabolize, destroy or inactivate those compounds. Laccases are enzymes with biocatalytic capacity and wide biotechnological application, including food industry. Particularly, laccases from fungi that cause white rot have been proposed for mycotoxin biotransformation. In the present work, the effectiveness of different fungal laccases in reducing AFs levels (AFB1, AFG1, AFB2 and AFG2) was evaluated under an in vitro assay. Laccase production by Phylum Basidiomycota strains was evaluated and 9 of them were selected to carry out in vitro decontamination tests. A concentration of 1 µg/mL of AFB1 and AFG1, and 0.25 µg/mL of AFB2 and AFG2 were used at concentrations of 5, 10, 15 and 20 U/mL of laccases contained in enzyme extracts, in addition to vanillic acid as redox mediator (1 and 10 mM). The strain Trametes sp. B7-IMICO-RC was able to degrade the 4 AFs at high levels under all the evaluated conditions. The highest degradation percentage was reached in presence of 1 mM vanillic acid and 20 U/mL laccase (88, 99, 87 and 70%, for AFB1, AFG1, AFB2 and AFG2, respectively). An additional in vitro degradation assay of AFs was carried out using mosto as the substrate. The degradation percentages observed under these same conditions were 26% for AFB1 and AFG2 and 54% for AFG1. Currently, studies are being carried out in order to determine the degradation products toxicity. These results would contribute to the development of an eco-friendly strategy to reduce mycotoxin contamination, ensuring food safety. Fil: Bossa, Marianela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina Fil: Monesterolo, Noelia Edith. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Monge, Maria del Pilar. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina Fil: Palazzini, Juan Manuel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina Fil: Pena, Gabriela Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina Fil: Chulza, Sofia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina Fil: Alaniz Zanon, Maria Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina Fil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina 14th conference of the World Mycotoxin Forum Amberes Bélgica Committee Organisation of 14th conference of the World Mycotoxin Forum |
| description |
Fungal contamination is a phytosanitary problem of concern in maize and by-products. Aspergillus section Flavi strains can generate important problems due to contamination with aflatoxins (AFs). During the bioethanol production from maize, AFs levels can increase up to three times in the final co-product (DDGS or WDGS), intended for animal feed. Mosto is the milled maize grains with the addition of water and amylase enzymes that enter the fermentation stage. One strategy to reduce the mycotoxin contamination levels is the use of microorganisms or enzymes able to metabolize, destroy or inactivate those compounds. Laccases are enzymes with biocatalytic capacity and wide biotechnological application, including food industry. Particularly, laccases from fungi that cause white rot have been proposed for mycotoxin biotransformation. In the present work, the effectiveness of different fungal laccases in reducing AFs levels (AFB1, AFG1, AFB2 and AFG2) was evaluated under an in vitro assay. Laccase production by Phylum Basidiomycota strains was evaluated and 9 of them were selected to carry out in vitro decontamination tests. A concentration of 1 µg/mL of AFB1 and AFG1, and 0.25 µg/mL of AFB2 and AFG2 were used at concentrations of 5, 10, 15 and 20 U/mL of laccases contained in enzyme extracts, in addition to vanillic acid as redox mediator (1 and 10 mM). The strain Trametes sp. B7-IMICO-RC was able to degrade the 4 AFs at high levels under all the evaluated conditions. The highest degradation percentage was reached in presence of 1 mM vanillic acid and 20 U/mL laccase (88, 99, 87 and 70%, for AFB1, AFG1, AFB2 and AFG2, respectively). An additional in vitro degradation assay of AFs was carried out using mosto as the substrate. The degradation percentages observed under these same conditions were 26% for AFB1 and AFG2 and 54% for AFG1. Currently, studies are being carried out in order to determine the degradation products toxicity. These results would contribute to the development of an eco-friendly strategy to reduce mycotoxin contamination, ensuring food safety. |
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2023 |
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Use of fungal laccases for aflatoxin reduction in maize sub-products; 14th conference of the World Mycotoxin Forum; Amberes; Bélgica; 2023; 128-128 CONICET Digital CONICET |
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