The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions

Autores
Falcone Ferreyra, María Lorena; Emiliani, Julia; Rodriguez, Eduardo Jose; Campos Bermudez, Valeria Alina; Grotewold, Erich; Casati, Paula
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flavones are a major group of flavonoids with diverse functions and extensively distributed in land plants. There are two different classes of flavone synthase (FNS) enzymes that catalyze the conversion of the flavanones into flavones. The FNSI class comprises soluble Fe2+/2-oxoglutarate-dependent dioxygenases, and FNSII enzymes are oxygen- and NADPH-dependent cytochrome P450 membrane-bound monooxygenases. Here, we describe the identification and characterization of two FNSI enzymes from Zea mays and Arabidopsis thaliana. In maize, ZmFNSI-1 is expressed at significantly higher levels in silks and pericarps expressing the 3-deoxy flavonoid R2R3-MYB regulator P1, suggesting that ZmFNSI-1 could be the main enzyme for the synthesis of flavone O-glycosides. We also show here that AtDMR6, the Arabidopsis homologous enzyme to ZmFNSI-1, has FNSI activity; dmr6 mutants show loss of susceptibility to Hyaloperonospora parasitica and other pathogens. AtDMR6 expression analysis showed a tissue and developmental stage-dependent pattern, with high expression in cauline and senescing leaves. Finally, we show that Arabidopsis cauline and senescing leaves accumulate apigenin, demonstrating that Arabidopsis thaliana plants have a FNSI activity involved in the biosynthesis of flavones. The results presented here also suggest a cross-talk between the flavone and salicylic acid pathways in Arabidopsis; in this way, pathogens would induce flavones to decrease salicylic acid and hence increase susceptibility.
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Emiliani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Rodriguez, Eduardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Campos Bermudez, Valeria Alina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Grotewold, Erich. Ohio State University; Estados Unidos
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Materia
Flavonas
Maiz
Arabidopsis
Estrés
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7862
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactionsFalcone Ferreyra, María LorenaEmiliani, JuliaRodriguez, Eduardo JoseCampos Bermudez, Valeria AlinaGrotewold, ErichCasati, PaulaFlavonasMaizArabidopsisEstréshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flavones are a major group of flavonoids with diverse functions and extensively distributed in land plants. There are two different classes of flavone synthase (FNS) enzymes that catalyze the conversion of the flavanones into flavones. The FNSI class comprises soluble Fe2+/2-oxoglutarate-dependent dioxygenases, and FNSII enzymes are oxygen- and NADPH-dependent cytochrome P450 membrane-bound monooxygenases. Here, we describe the identification and characterization of two FNSI enzymes from Zea mays and Arabidopsis thaliana. In maize, ZmFNSI-1 is expressed at significantly higher levels in silks and pericarps expressing the 3-deoxy flavonoid R2R3-MYB regulator P1, suggesting that ZmFNSI-1 could be the main enzyme for the synthesis of flavone O-glycosides. We also show here that AtDMR6, the Arabidopsis homologous enzyme to ZmFNSI-1, has FNSI activity; dmr6 mutants show loss of susceptibility to Hyaloperonospora parasitica and other pathogens. AtDMR6 expression analysis showed a tissue and developmental stage-dependent pattern, with high expression in cauline and senescing leaves. Finally, we show that Arabidopsis cauline and senescing leaves accumulate apigenin, demonstrating that Arabidopsis thaliana plants have a FNSI activity involved in the biosynthesis of flavones. The results presented here also suggest a cross-talk between the flavone and salicylic acid pathways in Arabidopsis; in this way, pathogens would induce flavones to decrease salicylic acid and hence increase susceptibility.Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Emiliani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Rodriguez, Eduardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Campos Bermudez, Valeria Alina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Grotewold, Erich. Ohio State University; Estados UnidosFil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaAmerican Society Of Plant Biologist2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7862Falcone Ferreyra, María Lorena; Emiliani, Julia; Rodriguez, Eduardo Jose; Campos Bermudez, Valeria Alina; Grotewold, Erich; et al.; The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions; American Society Of Plant Biologist; Plant Physiology.; 169; 2; 9-2015; 1090-11070032-0889enginfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.15.00515info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/169/2/1090/6114074info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:38Zoai:ri.conicet.gov.ar:11336/7862instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:38.678CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions
title The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions
spellingShingle The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions
Falcone Ferreyra, María Lorena
Flavonas
Maiz
Arabidopsis
Estrés
title_short The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions
title_full The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions
title_fullStr The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions
title_full_unstemmed The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions
title_sort The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions
dc.creator.none.fl_str_mv Falcone Ferreyra, María Lorena
Emiliani, Julia
Rodriguez, Eduardo Jose
Campos Bermudez, Valeria Alina
Grotewold, Erich
Casati, Paula
author Falcone Ferreyra, María Lorena
author_facet Falcone Ferreyra, María Lorena
Emiliani, Julia
Rodriguez, Eduardo Jose
Campos Bermudez, Valeria Alina
Grotewold, Erich
Casati, Paula
author_role author
author2 Emiliani, Julia
Rodriguez, Eduardo Jose
Campos Bermudez, Valeria Alina
Grotewold, Erich
Casati, Paula
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Flavonas
Maiz
Arabidopsis
Estrés
topic Flavonas
Maiz
Arabidopsis
Estrés
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flavones are a major group of flavonoids with diverse functions and extensively distributed in land plants. There are two different classes of flavone synthase (FNS) enzymes that catalyze the conversion of the flavanones into flavones. The FNSI class comprises soluble Fe2+/2-oxoglutarate-dependent dioxygenases, and FNSII enzymes are oxygen- and NADPH-dependent cytochrome P450 membrane-bound monooxygenases. Here, we describe the identification and characterization of two FNSI enzymes from Zea mays and Arabidopsis thaliana. In maize, ZmFNSI-1 is expressed at significantly higher levels in silks and pericarps expressing the 3-deoxy flavonoid R2R3-MYB regulator P1, suggesting that ZmFNSI-1 could be the main enzyme for the synthesis of flavone O-glycosides. We also show here that AtDMR6, the Arabidopsis homologous enzyme to ZmFNSI-1, has FNSI activity; dmr6 mutants show loss of susceptibility to Hyaloperonospora parasitica and other pathogens. AtDMR6 expression analysis showed a tissue and developmental stage-dependent pattern, with high expression in cauline and senescing leaves. Finally, we show that Arabidopsis cauline and senescing leaves accumulate apigenin, demonstrating that Arabidopsis thaliana plants have a FNSI activity involved in the biosynthesis of flavones. The results presented here also suggest a cross-talk between the flavone and salicylic acid pathways in Arabidopsis; in this way, pathogens would induce flavones to decrease salicylic acid and hence increase susceptibility.
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Emiliani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Rodriguez, Eduardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Campos Bermudez, Valeria Alina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Grotewold, Erich. Ohio State University; Estados Unidos
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
description Flavones are a major group of flavonoids with diverse functions and extensively distributed in land plants. There are two different classes of flavone synthase (FNS) enzymes that catalyze the conversion of the flavanones into flavones. The FNSI class comprises soluble Fe2+/2-oxoglutarate-dependent dioxygenases, and FNSII enzymes are oxygen- and NADPH-dependent cytochrome P450 membrane-bound monooxygenases. Here, we describe the identification and characterization of two FNSI enzymes from Zea mays and Arabidopsis thaliana. In maize, ZmFNSI-1 is expressed at significantly higher levels in silks and pericarps expressing the 3-deoxy flavonoid R2R3-MYB regulator P1, suggesting that ZmFNSI-1 could be the main enzyme for the synthesis of flavone O-glycosides. We also show here that AtDMR6, the Arabidopsis homologous enzyme to ZmFNSI-1, has FNSI activity; dmr6 mutants show loss of susceptibility to Hyaloperonospora parasitica and other pathogens. AtDMR6 expression analysis showed a tissue and developmental stage-dependent pattern, with high expression in cauline and senescing leaves. Finally, we show that Arabidopsis cauline and senescing leaves accumulate apigenin, demonstrating that Arabidopsis thaliana plants have a FNSI activity involved in the biosynthesis of flavones. The results presented here also suggest a cross-talk between the flavone and salicylic acid pathways in Arabidopsis; in this way, pathogens would induce flavones to decrease salicylic acid and hence increase susceptibility.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7862
Falcone Ferreyra, María Lorena; Emiliani, Julia; Rodriguez, Eduardo Jose; Campos Bermudez, Valeria Alina; Grotewold, Erich; et al.; The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions; American Society Of Plant Biologist; Plant Physiology.; 169; 2; 9-2015; 1090-1107
0032-0889
url http://hdl.handle.net/11336/7862
identifier_str_mv Falcone Ferreyra, María Lorena; Emiliani, Julia; Rodriguez, Eduardo Jose; Campos Bermudez, Valeria Alina; Grotewold, Erich; et al.; The identification of maize and Arabidopsis type I flavone synthases links flavones with hormones and biotic interactions; American Society Of Plant Biologist; Plant Physiology.; 169; 2; 9-2015; 1090-1107
0032-0889
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.15.00515
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/169/2/1090/6114074
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society Of Plant Biologist
publisher.none.fl_str_mv American Society Of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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