Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spe...

Autores
Chang, Fang-Yu; Hatakeyma, Yuto; Nonami, Hiroshi; Erra Balsells, Rosa; Araki, Takuya; Nakano, Hiroshi; Wada, Hiroshi
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High night temperature (HNT) at the ripening stage severely affects both rice yield and quality. HNT accelerates embryo growth and chalky formation in the developing grains, accompanying with a diminishment of endosperm cell size. Although these responses may be physiologically interacted each other in the grains, what signals are involved in the accelerated embryo development remains undetermined. In this work, we have used picolitre pressure-probe electrospray-ionization mass spectrometry (picoPPESI-MS) to conduct single-cell metabolomics at several regions in HNT-treated grains, embryonic scutellum and outer endosperms in the basal (‘chalky region’ at maturation) and middle (‘translucent region’ at maturation as a reference) positions. Microscopic observations showed that HNT promoted cell expansion rate in the scutellum. When embryonic cell expansion rate reached the maximum, spatial differences in several metabolisms including ascorbate-glutathione (ASC-GSH) pathway and purine were detected, together with considerable sugar and amino acid accumulations in embryonic scutellum cells. There was no treatment difference in GSH content during active cell expansion in HNT-treated embryos, although an increase in GSH/GSSG ratio due to a reduction in oxidized glutathione (GSSG) content has been contrastingly observed. In the endosperms, greater ASC accumulation with a difference in ASC/dehydroascobic acid ratio has been also detected under HNT conditions. Since dormancy is often correlated with GSSG concentration, it is concluded that spatial regulation of GSH redox homeostasis detected at cell-level might be essential for dormancy alleviation and embryo growth accelerated in HNT-treated seeds.
Fil: Chang, Fang-Yu. Ehime University; Japón
Fil: Hatakeyma, Yuto. Ehime University; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Araki, Takuya. Ehime University; Japón
Fil: Nakano, Hiroshi. Ehime University; Japón
Fil: Wada, Hiroshi. Ehime University; Japón
Materia
ASCORBIC ACID
CHALKINESS
EMBRYO DEVELOPMENT
GLUTATHIONE
HIGH NIGHT TEMPERATURE
ORYZA SATIVA
REDOX
SINGLE-CELL ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181301
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181301
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometryChang, Fang-YuHatakeyma, YutoNonami, HiroshiErra Balsells, RosaAraki, TakuyaNakano, HiroshiWada, HiroshiASCORBIC ACIDCHALKINESSEMBRYO DEVELOPMENTGLUTATHIONEHIGH NIGHT TEMPERATUREORYZA SATIVAREDOXSINGLE-CELL ANALYSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1High night temperature (HNT) at the ripening stage severely affects both rice yield and quality. HNT accelerates embryo growth and chalky formation in the developing grains, accompanying with a diminishment of endosperm cell size. Although these responses may be physiologically interacted each other in the grains, what signals are involved in the accelerated embryo development remains undetermined. In this work, we have used picolitre pressure-probe electrospray-ionization mass spectrometry (picoPPESI-MS) to conduct single-cell metabolomics at several regions in HNT-treated grains, embryonic scutellum and outer endosperms in the basal (‘chalky region’ at maturation) and middle (‘translucent region’ at maturation as a reference) positions. Microscopic observations showed that HNT promoted cell expansion rate in the scutellum. When embryonic cell expansion rate reached the maximum, spatial differences in several metabolisms including ascorbate-glutathione (ASC-GSH) pathway and purine were detected, together with considerable sugar and amino acid accumulations in embryonic scutellum cells. There was no treatment difference in GSH content during active cell expansion in HNT-treated embryos, although an increase in GSH/GSSG ratio due to a reduction in oxidized glutathione (GSSG) content has been contrastingly observed. In the endosperms, greater ASC accumulation with a difference in ASC/dehydroascobic acid ratio has been also detected under HNT conditions. Since dormancy is often correlated with GSSG concentration, it is concluded that spatial regulation of GSH redox homeostasis detected at cell-level might be essential for dormancy alleviation and embryo growth accelerated in HNT-treated seeds.Fil: Chang, Fang-Yu. Ehime University; JapónFil: Hatakeyma, Yuto. Ehime University; JapónFil: Nonami, Hiroshi. Ehime University; JapónFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Araki, Takuya. Ehime University; JapónFil: Nakano, Hiroshi. Ehime University; JapónFil: Wada, Hiroshi. Ehime University; JapónPergamon-Elsevier Science Ltd2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181301Chang, Fang-Yu; Hatakeyma, Yuto; Nonami, Hiroshi; Erra Balsells, Rosa; Araki, Takuya; et al.; Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 188; 5-2021; 104515-1045300098-8472CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2021.104515info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:49Zoai:ri.conicet.gov.ar:11336/181301instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:49.824CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry
title Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry
spellingShingle Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry
Chang, Fang-Yu
ASCORBIC ACID
CHALKINESS
EMBRYO DEVELOPMENT
GLUTATHIONE
HIGH NIGHT TEMPERATURE
ORYZA SATIVA
REDOX
SINGLE-CELL ANALYSIS
title_short Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry
title_full Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry
title_fullStr Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry
title_full_unstemmed Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry
title_sort Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry
dc.creator.none.fl_str_mv Chang, Fang-Yu
Hatakeyma, Yuto
Nonami, Hiroshi
Erra Balsells, Rosa
Araki, Takuya
Nakano, Hiroshi
Wada, Hiroshi
author Chang, Fang-Yu
author_facet Chang, Fang-Yu
Hatakeyma, Yuto
Nonami, Hiroshi
Erra Balsells, Rosa
Araki, Takuya
Nakano, Hiroshi
Wada, Hiroshi
author_role author
author2 Hatakeyma, Yuto
Nonami, Hiroshi
Erra Balsells, Rosa
Araki, Takuya
Nakano, Hiroshi
Wada, Hiroshi
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ASCORBIC ACID
CHALKINESS
EMBRYO DEVELOPMENT
GLUTATHIONE
HIGH NIGHT TEMPERATURE
ORYZA SATIVA
REDOX
SINGLE-CELL ANALYSIS
topic ASCORBIC ACID
CHALKINESS
EMBRYO DEVELOPMENT
GLUTATHIONE
HIGH NIGHT TEMPERATURE
ORYZA SATIVA
REDOX
SINGLE-CELL ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv High night temperature (HNT) at the ripening stage severely affects both rice yield and quality. HNT accelerates embryo growth and chalky formation in the developing grains, accompanying with a diminishment of endosperm cell size. Although these responses may be physiologically interacted each other in the grains, what signals are involved in the accelerated embryo development remains undetermined. In this work, we have used picolitre pressure-probe electrospray-ionization mass spectrometry (picoPPESI-MS) to conduct single-cell metabolomics at several regions in HNT-treated grains, embryonic scutellum and outer endosperms in the basal (‘chalky region’ at maturation) and middle (‘translucent region’ at maturation as a reference) positions. Microscopic observations showed that HNT promoted cell expansion rate in the scutellum. When embryonic cell expansion rate reached the maximum, spatial differences in several metabolisms including ascorbate-glutathione (ASC-GSH) pathway and purine were detected, together with considerable sugar and amino acid accumulations in embryonic scutellum cells. There was no treatment difference in GSH content during active cell expansion in HNT-treated embryos, although an increase in GSH/GSSG ratio due to a reduction in oxidized glutathione (GSSG) content has been contrastingly observed. In the endosperms, greater ASC accumulation with a difference in ASC/dehydroascobic acid ratio has been also detected under HNT conditions. Since dormancy is often correlated with GSSG concentration, it is concluded that spatial regulation of GSH redox homeostasis detected at cell-level might be essential for dormancy alleviation and embryo growth accelerated in HNT-treated seeds.
Fil: Chang, Fang-Yu. Ehime University; Japón
Fil: Hatakeyma, Yuto. Ehime University; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Araki, Takuya. Ehime University; Japón
Fil: Nakano, Hiroshi. Ehime University; Japón
Fil: Wada, Hiroshi. Ehime University; Japón
description High night temperature (HNT) at the ripening stage severely affects both rice yield and quality. HNT accelerates embryo growth and chalky formation in the developing grains, accompanying with a diminishment of endosperm cell size. Although these responses may be physiologically interacted each other in the grains, what signals are involved in the accelerated embryo development remains undetermined. In this work, we have used picolitre pressure-probe electrospray-ionization mass spectrometry (picoPPESI-MS) to conduct single-cell metabolomics at several regions in HNT-treated grains, embryonic scutellum and outer endosperms in the basal (‘chalky region’ at maturation) and middle (‘translucent region’ at maturation as a reference) positions. Microscopic observations showed that HNT promoted cell expansion rate in the scutellum. When embryonic cell expansion rate reached the maximum, spatial differences in several metabolisms including ascorbate-glutathione (ASC-GSH) pathway and purine were detected, together with considerable sugar and amino acid accumulations in embryonic scutellum cells. There was no treatment difference in GSH content during active cell expansion in HNT-treated embryos, although an increase in GSH/GSSG ratio due to a reduction in oxidized glutathione (GSSG) content has been contrastingly observed. In the endosperms, greater ASC accumulation with a difference in ASC/dehydroascobic acid ratio has been also detected under HNT conditions. Since dormancy is often correlated with GSSG concentration, it is concluded that spatial regulation of GSH redox homeostasis detected at cell-level might be essential for dormancy alleviation and embryo growth accelerated in HNT-treated seeds.
publishDate 2021
dc.date.none.fl_str_mv 2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181301
Chang, Fang-Yu; Hatakeyma, Yuto; Nonami, Hiroshi; Erra Balsells, Rosa; Araki, Takuya; et al.; Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 188; 5-2021; 104515-104530
0098-8472
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181301
identifier_str_mv Chang, Fang-Yu; Hatakeyma, Yuto; Nonami, Hiroshi; Erra Balsells, Rosa; Araki, Takuya; et al.; Metabolic coordination of rice seed development to nighttime warming: In-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 188; 5-2021; 104515-104530
0098-8472
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2021.104515
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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