Histamine prevents radiation-induced mesenchymal changes in breast cancer cells

Autores
Galarza, Tamara Ester; Mohamad, Nora Alicia; Táquez Delgado, Mónica Alejandra; Vedoya, Guadalupe M.; Crescenti, Ernesto J.; Bergoc, Rosa Maria; Martin, Gabriela Adriana; Cricco, Graciela Patricia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Radiotherapy is a prime option for treatment of solid tumors including breast cancer though side effects are usually present. Experimental evidence shows an increase in invasiveness of several neoplastic cell types through conventional tumor irradiation. The induction of epithelial to mesenchymal transition is proposed as an underlying cause of metastasis triggered by gamma irradiation. Experiments were conducted to investigate the role of histamine on the ionizing radiation-induced epithelial to mesenchymal transition events in breast cancer cells with different invasive phenotype. We also evaluated the potential involvement of Src phosphorylation in the migratory capability of irradiated cells upon histamine treatment. MCF-7 and MDA-MB-231 mammary tumor cells were exposed to a single dose of 2 Gy of gamma radiation and five days after irradiation mesenchymal-like phenotypic changes were observed by optical microscope. The expression and subcellular localization of E-cadherin, β-catenin, vimentin and Slug were determined by immunoblot and indirect immunofluorescence. There was a decrease in the epithelial marker E-cadherin expression and an increase in the mesenchymal marker vimentin after irradiation. E-cadherin and β-catenin were mainly localized in cytoplasm. Slug positive nuclei, matrix metalloproteinase-2 activity and cell migration and invasion were significantly increased. In addition, a significant enhancement in Src phosphorylation/activation could be determined by immunoblot in irradiated cells. MCF-7 and MDA-MB-231 cells also received 1 or 20 μM histamine during 24 h previous to be irradiated. Notably, pre-treatment of breast cancer cells with 20 μM histamine prevented the mesenchymal changes induced by ionizing radiation and also reduced the migratory behavior of irradiated cells decreasing phospho-Src levels. Collectively, our results suggest that histamine may block events related to epithelial to mesenchymal transition in irradiated mammary cancer cells and open a perspective for the potential use of histamine to improve radiotherapy efficacy.
Fil: Galarza, Tamara Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Mohamad, Nora Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Táquez Delgado, Mónica Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vedoya, Guadalupe M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Crescenti, Ernesto J.. Instituto de Inmunooncología; Argentina
Fil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martin, Gabriela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cricco, Graciela Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Breast Cancer
Epithelial to Mesenchymal Transition
Histamine
Ionizing Radiation
Src
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39370
Acceder
id CONICETDig_8959d9f51e7c742af9a5df83049e962f
oai_identifier_str oai:ri.conicet.gov.ar:11336/39370
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Histamine prevents radiation-induced mesenchymal changes in breast cancer cellsGalarza, Tamara EsterMohamad, Nora AliciaTáquez Delgado, Mónica AlejandraVedoya, Guadalupe M.Crescenti, Ernesto J.Bergoc, Rosa MariaMartin, Gabriela AdrianaCricco, Graciela PatriciaBreast CancerEpithelial to Mesenchymal TransitionHistamineIonizing RadiationSrchttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Radiotherapy is a prime option for treatment of solid tumors including breast cancer though side effects are usually present. Experimental evidence shows an increase in invasiveness of several neoplastic cell types through conventional tumor irradiation. The induction of epithelial to mesenchymal transition is proposed as an underlying cause of metastasis triggered by gamma irradiation. Experiments were conducted to investigate the role of histamine on the ionizing radiation-induced epithelial to mesenchymal transition events in breast cancer cells with different invasive phenotype. We also evaluated the potential involvement of Src phosphorylation in the migratory capability of irradiated cells upon histamine treatment. MCF-7 and MDA-MB-231 mammary tumor cells were exposed to a single dose of 2 Gy of gamma radiation and five days after irradiation mesenchymal-like phenotypic changes were observed by optical microscope. The expression and subcellular localization of E-cadherin, β-catenin, vimentin and Slug were determined by immunoblot and indirect immunofluorescence. There was a decrease in the epithelial marker E-cadherin expression and an increase in the mesenchymal marker vimentin after irradiation. E-cadherin and β-catenin were mainly localized in cytoplasm. Slug positive nuclei, matrix metalloproteinase-2 activity and cell migration and invasion were significantly increased. In addition, a significant enhancement in Src phosphorylation/activation could be determined by immunoblot in irradiated cells. MCF-7 and MDA-MB-231 cells also received 1 or 20 μM histamine during 24 h previous to be irradiated. Notably, pre-treatment of breast cancer cells with 20 μM histamine prevented the mesenchymal changes induced by ionizing radiation and also reduced the migratory behavior of irradiated cells decreasing phospho-Src levels. Collectively, our results suggest that histamine may block events related to epithelial to mesenchymal transition in irradiated mammary cancer cells and open a perspective for the potential use of histamine to improve radiotherapy efficacy.Fil: Galarza, Tamara Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Mohamad, Nora Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Táquez Delgado, Mónica Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vedoya, Guadalupe M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Crescenti, Ernesto J.. Instituto de Inmunooncología; ArgentinaFil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martin, Gabriela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Cricco, Graciela Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39370Galarza, Tamara Ester; Mohamad, Nora Alicia; Táquez Delgado, Mónica Alejandra; Vedoya, Guadalupe M.; Crescenti, Ernesto J.; et al.; Histamine prevents radiation-induced mesenchymal changes in breast cancer cells; Academic Press Ltd - Elsevier Science Ltd; Pharmacological Research; 111; 9-2016; 731-7391043-6618CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.phrs.2016.07.039info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1043661816307216info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:14Zoai:ri.conicet.gov.ar:11336/39370instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:14.606CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histamine prevents radiation-induced mesenchymal changes in breast cancer cells
title Histamine prevents radiation-induced mesenchymal changes in breast cancer cells
spellingShingle Histamine prevents radiation-induced mesenchymal changes in breast cancer cells
Galarza, Tamara Ester
Breast Cancer
Epithelial to Mesenchymal Transition
Histamine
Ionizing Radiation
Src
title_short Histamine prevents radiation-induced mesenchymal changes in breast cancer cells
title_full Histamine prevents radiation-induced mesenchymal changes in breast cancer cells
title_fullStr Histamine prevents radiation-induced mesenchymal changes in breast cancer cells
title_full_unstemmed Histamine prevents radiation-induced mesenchymal changes in breast cancer cells
title_sort Histamine prevents radiation-induced mesenchymal changes in breast cancer cells
dc.creator.none.fl_str_mv Galarza, Tamara Ester
Mohamad, Nora Alicia
Táquez Delgado, Mónica Alejandra
Vedoya, Guadalupe M.
Crescenti, Ernesto J.
Bergoc, Rosa Maria
Martin, Gabriela Adriana
Cricco, Graciela Patricia
author Galarza, Tamara Ester
author_facet Galarza, Tamara Ester
Mohamad, Nora Alicia
Táquez Delgado, Mónica Alejandra
Vedoya, Guadalupe M.
Crescenti, Ernesto J.
Bergoc, Rosa Maria
Martin, Gabriela Adriana
Cricco, Graciela Patricia
author_role author
author2 Mohamad, Nora Alicia
Táquez Delgado, Mónica Alejandra
Vedoya, Guadalupe M.
Crescenti, Ernesto J.
Bergoc, Rosa Maria
Martin, Gabriela Adriana
Cricco, Graciela Patricia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Breast Cancer
Epithelial to Mesenchymal Transition
Histamine
Ionizing Radiation
Src
topic Breast Cancer
Epithelial to Mesenchymal Transition
Histamine
Ionizing Radiation
Src
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Radiotherapy is a prime option for treatment of solid tumors including breast cancer though side effects are usually present. Experimental evidence shows an increase in invasiveness of several neoplastic cell types through conventional tumor irradiation. The induction of epithelial to mesenchymal transition is proposed as an underlying cause of metastasis triggered by gamma irradiation. Experiments were conducted to investigate the role of histamine on the ionizing radiation-induced epithelial to mesenchymal transition events in breast cancer cells with different invasive phenotype. We also evaluated the potential involvement of Src phosphorylation in the migratory capability of irradiated cells upon histamine treatment. MCF-7 and MDA-MB-231 mammary tumor cells were exposed to a single dose of 2 Gy of gamma radiation and five days after irradiation mesenchymal-like phenotypic changes were observed by optical microscope. The expression and subcellular localization of E-cadherin, β-catenin, vimentin and Slug were determined by immunoblot and indirect immunofluorescence. There was a decrease in the epithelial marker E-cadherin expression and an increase in the mesenchymal marker vimentin after irradiation. E-cadherin and β-catenin were mainly localized in cytoplasm. Slug positive nuclei, matrix metalloproteinase-2 activity and cell migration and invasion were significantly increased. In addition, a significant enhancement in Src phosphorylation/activation could be determined by immunoblot in irradiated cells. MCF-7 and MDA-MB-231 cells also received 1 or 20 μM histamine during 24 h previous to be irradiated. Notably, pre-treatment of breast cancer cells with 20 μM histamine prevented the mesenchymal changes induced by ionizing radiation and also reduced the migratory behavior of irradiated cells decreasing phospho-Src levels. Collectively, our results suggest that histamine may block events related to epithelial to mesenchymal transition in irradiated mammary cancer cells and open a perspective for the potential use of histamine to improve radiotherapy efficacy.
Fil: Galarza, Tamara Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Mohamad, Nora Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Táquez Delgado, Mónica Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vedoya, Guadalupe M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Crescenti, Ernesto J.. Instituto de Inmunooncología; Argentina
Fil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martin, Gabriela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cricco, Graciela Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Radiotherapy is a prime option for treatment of solid tumors including breast cancer though side effects are usually present. Experimental evidence shows an increase in invasiveness of several neoplastic cell types through conventional tumor irradiation. The induction of epithelial to mesenchymal transition is proposed as an underlying cause of metastasis triggered by gamma irradiation. Experiments were conducted to investigate the role of histamine on the ionizing radiation-induced epithelial to mesenchymal transition events in breast cancer cells with different invasive phenotype. We also evaluated the potential involvement of Src phosphorylation in the migratory capability of irradiated cells upon histamine treatment. MCF-7 and MDA-MB-231 mammary tumor cells were exposed to a single dose of 2 Gy of gamma radiation and five days after irradiation mesenchymal-like phenotypic changes were observed by optical microscope. The expression and subcellular localization of E-cadherin, β-catenin, vimentin and Slug were determined by immunoblot and indirect immunofluorescence. There was a decrease in the epithelial marker E-cadherin expression and an increase in the mesenchymal marker vimentin after irradiation. E-cadherin and β-catenin were mainly localized in cytoplasm. Slug positive nuclei, matrix metalloproteinase-2 activity and cell migration and invasion were significantly increased. In addition, a significant enhancement in Src phosphorylation/activation could be determined by immunoblot in irradiated cells. MCF-7 and MDA-MB-231 cells also received 1 or 20 μM histamine during 24 h previous to be irradiated. Notably, pre-treatment of breast cancer cells with 20 μM histamine prevented the mesenchymal changes induced by ionizing radiation and also reduced the migratory behavior of irradiated cells decreasing phospho-Src levels. Collectively, our results suggest that histamine may block events related to epithelial to mesenchymal transition in irradiated mammary cancer cells and open a perspective for the potential use of histamine to improve radiotherapy efficacy.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39370
Galarza, Tamara Ester; Mohamad, Nora Alicia; Táquez Delgado, Mónica Alejandra; Vedoya, Guadalupe M.; Crescenti, Ernesto J.; et al.; Histamine prevents radiation-induced mesenchymal changes in breast cancer cells; Academic Press Ltd - Elsevier Science Ltd; Pharmacological Research; 111; 9-2016; 731-739
1043-6618
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39370
identifier_str_mv Galarza, Tamara Ester; Mohamad, Nora Alicia; Táquez Delgado, Mónica Alejandra; Vedoya, Guadalupe M.; Crescenti, Ernesto J.; et al.; Histamine prevents radiation-induced mesenchymal changes in breast cancer cells; Academic Press Ltd - Elsevier Science Ltd; Pharmacological Research; 111; 9-2016; 731-739
1043-6618
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phrs.2016.07.039
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1043661816307216
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268651641634816
score 13.13397

Publicaciones similares