Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis

Autores
Palacios Gimenez, Octavio M.; Milani, Diogo; Lemos, Bernardo; Castillo, Elio Rodrigo Daniel; Marti, Dardo Andrea; Ramos, Erica; Cabral De Mello, Diogo C.; Martins, Cesar
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Neo-sex chromosome systems arose independently multiple times in evolution, presenting the remarkable characteristic of repetitive DNAs accumulation. Among grasshoppers, occurrence of neo-XY was repeatedly noticed in Melanoplinae. Here we analyzed the most abundant tandem repeats of R. bergii (2n = 22, neo-XY♂) using deep Illumina sequencing and graph-based clustering in order to address the neo-sex chromosomes evolution. Results: The analyses revealed ten families of satDNAs comprising about ~1% of the male genome, which occupied mainly C-positive regions of autosomes. Regarding the sex chromosomes, satDNAs were recorded within centromeric or interstitial regions of the neo-X chromosome and four satDNAs occurred in the neo-Y, two of them being exclusive (Rber248 and Rber299). Using a combination of probes we uncovered five well-defined cytological variants for neo-Y, originated by multiple paracentric inversions and satDNA amplification, besides fragmented neo-Y. These neo-Y variants were distinct in frequency between embryos and adult males. Conclusions: The genomic data together with cytogenetic mapping enabled us to better understand the neo-sex chromosome dynamics in grasshoppers, reinforcing differentiation of neo-X and neo-Y and revealing the occurrence of multiple additional rearrangements involved in the neo-Y evolution of R. bergii. We discussed the possible causes that led to differences in frequency for the neo-Y variants between embryos and adults. Finally we hypothesize about the role of DNA satellites in R. bergii as well as putative historical events involved in the evolution of the R. bergii neo-XY.
Fil: Palacios Gimenez, Octavio M.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Milani, Diogo. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Lemos, Bernardo. Harvard University. Harvard School of Public Health; Estados Unidos
Fil: Castillo, Elio Rodrigo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Ramos, Erica. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Cabral De Mello, Diogo C.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Martins, Cesar. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Materia
CHROMOSOMAL REARRANGEMENTS
EVOLUTION
FISH
SATELLITE DNA
SEX CHROMOSOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/86191
Acceder
id CONICETDig_cfb32a4397cc3fffcc0287a96db048b5
oai_identifier_str oai:ri.conicet.gov.ar:11336/86191
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysisPalacios Gimenez, Octavio M.Milani, DiogoLemos, BernardoCastillo, Elio Rodrigo DanielMarti, Dardo AndreaRamos, EricaCabral De Mello, Diogo C.Martins, CesarCHROMOSOMAL REARRANGEMENTSEVOLUTIONFISHSATELLITE DNASEX CHROMOSOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Neo-sex chromosome systems arose independently multiple times in evolution, presenting the remarkable characteristic of repetitive DNAs accumulation. Among grasshoppers, occurrence of neo-XY was repeatedly noticed in Melanoplinae. Here we analyzed the most abundant tandem repeats of R. bergii (2n = 22, neo-XY♂) using deep Illumina sequencing and graph-based clustering in order to address the neo-sex chromosomes evolution. Results: The analyses revealed ten families of satDNAs comprising about ~1% of the male genome, which occupied mainly C-positive regions of autosomes. Regarding the sex chromosomes, satDNAs were recorded within centromeric or interstitial regions of the neo-X chromosome and four satDNAs occurred in the neo-Y, two of them being exclusive (Rber248 and Rber299). Using a combination of probes we uncovered five well-defined cytological variants for neo-Y, originated by multiple paracentric inversions and satDNA amplification, besides fragmented neo-Y. These neo-Y variants were distinct in frequency between embryos and adult males. Conclusions: The genomic data together with cytogenetic mapping enabled us to better understand the neo-sex chromosome dynamics in grasshoppers, reinforcing differentiation of neo-X and neo-Y and revealing the occurrence of multiple additional rearrangements involved in the neo-Y evolution of R. bergii. We discussed the possible causes that led to differences in frequency for the neo-Y variants between embryos and adults. Finally we hypothesize about the role of DNA satellites in R. bergii as well as putative historical events involved in the evolution of the R. bergii neo-XY.Fil: Palacios Gimenez, Octavio M.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Milani, Diogo. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Lemos, Bernardo. Harvard University. Harvard School of Public Health; Estados UnidosFil: Castillo, Elio Rodrigo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Ramos, Erica. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Cabral De Mello, Diogo C.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Martins, Cesar. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilBioMed Central2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/86191Palacios Gimenez, Octavio M.; Milani, Diogo; Lemos, Bernardo; Castillo, Elio Rodrigo Daniel; Marti, Dardo Andrea; et al.; Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis; BioMed Central; BMC Evolutionary Biology; 18; 2; 1-2018; 1-101471-2148CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcevolbiol.biomedcentral.com/articles/10.1186/s12862-017-1113-xinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12862-017-1113-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:25:36Zoai:ri.conicet.gov.ar:11336/86191instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:25:36.601CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
spellingShingle Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
Palacios Gimenez, Octavio M.
CHROMOSOMAL REARRANGEMENTS
EVOLUTION
FISH
SATELLITE DNA
SEX CHROMOSOME
title_short Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title_full Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title_fullStr Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title_full_unstemmed Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title_sort Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
dc.creator.none.fl_str_mv Palacios Gimenez, Octavio M.
Milani, Diogo
Lemos, Bernardo
Castillo, Elio Rodrigo Daniel
Marti, Dardo Andrea
Ramos, Erica
Cabral De Mello, Diogo C.
Martins, Cesar
author Palacios Gimenez, Octavio M.
author_facet Palacios Gimenez, Octavio M.
Milani, Diogo
Lemos, Bernardo
Castillo, Elio Rodrigo Daniel
Marti, Dardo Andrea
Ramos, Erica
Cabral De Mello, Diogo C.
Martins, Cesar
author_role author
author2 Milani, Diogo
Lemos, Bernardo
Castillo, Elio Rodrigo Daniel
Marti, Dardo Andrea
Ramos, Erica
Cabral De Mello, Diogo C.
Martins, Cesar
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHROMOSOMAL REARRANGEMENTS
EVOLUTION
FISH
SATELLITE DNA
SEX CHROMOSOME
topic CHROMOSOMAL REARRANGEMENTS
EVOLUTION
FISH
SATELLITE DNA
SEX CHROMOSOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Neo-sex chromosome systems arose independently multiple times in evolution, presenting the remarkable characteristic of repetitive DNAs accumulation. Among grasshoppers, occurrence of neo-XY was repeatedly noticed in Melanoplinae. Here we analyzed the most abundant tandem repeats of R. bergii (2n = 22, neo-XY♂) using deep Illumina sequencing and graph-based clustering in order to address the neo-sex chromosomes evolution. Results: The analyses revealed ten families of satDNAs comprising about ~1% of the male genome, which occupied mainly C-positive regions of autosomes. Regarding the sex chromosomes, satDNAs were recorded within centromeric or interstitial regions of the neo-X chromosome and four satDNAs occurred in the neo-Y, two of them being exclusive (Rber248 and Rber299). Using a combination of probes we uncovered five well-defined cytological variants for neo-Y, originated by multiple paracentric inversions and satDNA amplification, besides fragmented neo-Y. These neo-Y variants were distinct in frequency between embryos and adult males. Conclusions: The genomic data together with cytogenetic mapping enabled us to better understand the neo-sex chromosome dynamics in grasshoppers, reinforcing differentiation of neo-X and neo-Y and revealing the occurrence of multiple additional rearrangements involved in the neo-Y evolution of R. bergii. We discussed the possible causes that led to differences in frequency for the neo-Y variants between embryos and adults. Finally we hypothesize about the role of DNA satellites in R. bergii as well as putative historical events involved in the evolution of the R. bergii neo-XY.
Fil: Palacios Gimenez, Octavio M.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Milani, Diogo. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Lemos, Bernardo. Harvard University. Harvard School of Public Health; Estados Unidos
Fil: Castillo, Elio Rodrigo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Ramos, Erica. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Cabral De Mello, Diogo C.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Martins, Cesar. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
description Background: Neo-sex chromosome systems arose independently multiple times in evolution, presenting the remarkable characteristic of repetitive DNAs accumulation. Among grasshoppers, occurrence of neo-XY was repeatedly noticed in Melanoplinae. Here we analyzed the most abundant tandem repeats of R. bergii (2n = 22, neo-XY♂) using deep Illumina sequencing and graph-based clustering in order to address the neo-sex chromosomes evolution. Results: The analyses revealed ten families of satDNAs comprising about ~1% of the male genome, which occupied mainly C-positive regions of autosomes. Regarding the sex chromosomes, satDNAs were recorded within centromeric or interstitial regions of the neo-X chromosome and four satDNAs occurred in the neo-Y, two of them being exclusive (Rber248 and Rber299). Using a combination of probes we uncovered five well-defined cytological variants for neo-Y, originated by multiple paracentric inversions and satDNA amplification, besides fragmented neo-Y. These neo-Y variants were distinct in frequency between embryos and adult males. Conclusions: The genomic data together with cytogenetic mapping enabled us to better understand the neo-sex chromosome dynamics in grasshoppers, reinforcing differentiation of neo-X and neo-Y and revealing the occurrence of multiple additional rearrangements involved in the neo-Y evolution of R. bergii. We discussed the possible causes that led to differences in frequency for the neo-Y variants between embryos and adults. Finally we hypothesize about the role of DNA satellites in R. bergii as well as putative historical events involved in the evolution of the R. bergii neo-XY.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/86191
Palacios Gimenez, Octavio M.; Milani, Diogo; Lemos, Bernardo; Castillo, Elio Rodrigo Daniel; Marti, Dardo Andrea; et al.; Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis; BioMed Central; BMC Evolutionary Biology; 18; 2; 1-2018; 1-10
1471-2148
CONICET Digital
CONICET
url http://hdl.handle.net/11336/86191
identifier_str_mv Palacios Gimenez, Octavio M.; Milani, Diogo; Lemos, Bernardo; Castillo, Elio Rodrigo Daniel; Marti, Dardo Andrea; et al.; Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis; BioMed Central; BMC Evolutionary Biology; 18; 2; 1-2018; 1-10
1471-2148
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcevolbiol.biomedcentral.com/articles/10.1186/s12862-017-1113-x
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12862-017-1113-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082692599250944
score 13.22299

Publicaciones similares