Cationic porphyrin derivatives for application in photodynamic therapy of cancer

Autores
Prack Mc Cormick, Bárbara Patricia; Pansa, Maria Florencia; Milla Sanabria, Laura Natalia; Carvalho, Carla Marisa Brito; Faustino, Maria Amparo Ferreira; Neves, Maria Graça P. M. S.; Cavaleiro, José A. S.; Vittar, Natalia Belen Rumie; Rivarola, Viviana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Current studies in photodynamic therapy (PDT) against cancer are focused on the development of new photosensitizers (PSs), with higher phototoxic action. The aim of this study was to compare the therapeutic efficiency of tri-cationic meso-substituted porphyrin derivatives (Tri-Py+–Me–PF, Tri-Py+–Me–Ph, Tri-Py+–Me–CO2Me and Tri-Py+–Me–CO2H) with the well-known tetra-cationic T4PM. The phototoxic action of these derivatives was assessed in human colon adenocarcinoma cells by cell viability, intracellular localization and nuclear morphology analysis. In the experimental conditions used we determined that after light activation –PF, –Ph and –CO2Me cause a more significant decline of cell viability compared to –CO2H and T4PM. These results suggest that the nature of the peripheral substituent influences the extent of cell photodamage. Moreover, we have demonstrated that PS concentration, physicochemical properties and further light activation determine the PDT response. All porphyrins were clearly localized as a punctuated pattern in the cytoplasm of the cells, and the PDT scheme resulted in apoptotic cell death after 3 h post-PDT. The tri-cationic porphyrin derivatives Tri-Py+–Me–PF, Tri-Py+–Me–Ph and Tri-Py+–Me–CO2Me showed a promising ability, making them good photosensitizer candidates for oncological PDT.
Fil: Prack Mc Cormick, Bárbara Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pansa, Maria Florencia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Milla Sanabria, Laura Natalia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carvalho, Carla Marisa Brito. University of Aveiro; Portugal
Fil: Faustino, Maria Amparo Ferreira. University of Aveiro; Portugal
Fil: Neves, Maria Graça P. M. S.. University of Aveiro; Portugal
Fil: Cavaleiro, José A. S.. University of Aveiro; Portugal
Fil: Vittar, Natalia Belen Rumie. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rivarola, Viviana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Terapia Fotodinamica
Cancer
Porfirinas
Apoptosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16854
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cationic porphyrin derivatives for application in photodynamic therapy of cancerPrack Mc Cormick, Bárbara PatriciaPansa, Maria FlorenciaMilla Sanabria, Laura NataliaCarvalho, Carla Marisa BritoFaustino, Maria Amparo FerreiraNeves, Maria Graça P. M. S.Cavaleiro, José A. S.Vittar, Natalia Belen RumieRivarola, VivianaTerapia FotodinamicaCancerPorfirinasApoptosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Current studies in photodynamic therapy (PDT) against cancer are focused on the development of new photosensitizers (PSs), with higher phototoxic action. The aim of this study was to compare the therapeutic efficiency of tri-cationic meso-substituted porphyrin derivatives (Tri-Py+–Me–PF, Tri-Py+–Me–Ph, Tri-Py+–Me–CO2Me and Tri-Py+–Me–CO2H) with the well-known tetra-cationic T4PM. The phototoxic action of these derivatives was assessed in human colon adenocarcinoma cells by cell viability, intracellular localization and nuclear morphology analysis. In the experimental conditions used we determined that after light activation –PF, –Ph and –CO2Me cause a more significant decline of cell viability compared to –CO2H and T4PM. These results suggest that the nature of the peripheral substituent influences the extent of cell photodamage. Moreover, we have demonstrated that PS concentration, physicochemical properties and further light activation determine the PDT response. All porphyrins were clearly localized as a punctuated pattern in the cytoplasm of the cells, and the PDT scheme resulted in apoptotic cell death after 3 h post-PDT. The tri-cationic porphyrin derivatives Tri-Py+–Me–PF, Tri-Py+–Me–Ph and Tri-Py+–Me–CO2Me showed a promising ability, making them good photosensitizer candidates for oncological PDT.Fil: Prack Mc Cormick, Bárbara Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pansa, Maria Florencia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Milla Sanabria, Laura Natalia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carvalho, Carla Marisa Brito. University of Aveiro; PortugalFil: Faustino, Maria Amparo Ferreira. University of Aveiro; PortugalFil: Neves, Maria Graça P. M. S.. University of Aveiro; PortugalFil: Cavaleiro, José A. S.. University of Aveiro; PortugalFil: Vittar, Natalia Belen Rumie. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Rivarola, Viviana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaIOP Publishing2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16854Prack Mc Cormick, Bárbara Patricia; Pansa, Maria Florencia; Milla Sanabria, Laura Natalia; Carvalho, Carla Marisa Brito; Faustino, Maria Amparo Ferreira; et al.; Cationic porphyrin derivatives for application in photodynamic therapy of cancer; IOP Publishing; Laser Physics; 24; 4; 2-2014; 1-81054-660Xenginfo:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1054-660X/24/4/045603/meta;jsessionid=238DA785B6911E74D01395FCCF0DDFF2.ip-10-40-1-105#topinfo:eu-repo/semantics/altIdentifier/doi/10.1088/1054-660X/24/4/045603info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:56Zoai:ri.conicet.gov.ar:11336/16854instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:56.658CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cationic porphyrin derivatives for application in photodynamic therapy of cancer
title Cationic porphyrin derivatives for application in photodynamic therapy of cancer
spellingShingle Cationic porphyrin derivatives for application in photodynamic therapy of cancer
Prack Mc Cormick, Bárbara Patricia
Terapia Fotodinamica
Cancer
Porfirinas
Apoptosis
title_short Cationic porphyrin derivatives for application in photodynamic therapy of cancer
title_full Cationic porphyrin derivatives for application in photodynamic therapy of cancer
title_fullStr Cationic porphyrin derivatives for application in photodynamic therapy of cancer
title_full_unstemmed Cationic porphyrin derivatives for application in photodynamic therapy of cancer
title_sort Cationic porphyrin derivatives for application in photodynamic therapy of cancer
dc.creator.none.fl_str_mv Prack Mc Cormick, Bárbara Patricia
Pansa, Maria Florencia
Milla Sanabria, Laura Natalia
Carvalho, Carla Marisa Brito
Faustino, Maria Amparo Ferreira
Neves, Maria Graça P. M. S.
Cavaleiro, José A. S.
Vittar, Natalia Belen Rumie
Rivarola, Viviana
author Prack Mc Cormick, Bárbara Patricia
author_facet Prack Mc Cormick, Bárbara Patricia
Pansa, Maria Florencia
Milla Sanabria, Laura Natalia
Carvalho, Carla Marisa Brito
Faustino, Maria Amparo Ferreira
Neves, Maria Graça P. M. S.
Cavaleiro, José A. S.
Vittar, Natalia Belen Rumie
Rivarola, Viviana
author_role author
author2 Pansa, Maria Florencia
Milla Sanabria, Laura Natalia
Carvalho, Carla Marisa Brito
Faustino, Maria Amparo Ferreira
Neves, Maria Graça P. M. S.
Cavaleiro, José A. S.
Vittar, Natalia Belen Rumie
Rivarola, Viviana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Terapia Fotodinamica
Cancer
Porfirinas
Apoptosis
topic Terapia Fotodinamica
Cancer
Porfirinas
Apoptosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Current studies in photodynamic therapy (PDT) against cancer are focused on the development of new photosensitizers (PSs), with higher phototoxic action. The aim of this study was to compare the therapeutic efficiency of tri-cationic meso-substituted porphyrin derivatives (Tri-Py+–Me–PF, Tri-Py+–Me–Ph, Tri-Py+–Me–CO2Me and Tri-Py+–Me–CO2H) with the well-known tetra-cationic T4PM. The phototoxic action of these derivatives was assessed in human colon adenocarcinoma cells by cell viability, intracellular localization and nuclear morphology analysis. In the experimental conditions used we determined that after light activation –PF, –Ph and –CO2Me cause a more significant decline of cell viability compared to –CO2H and T4PM. These results suggest that the nature of the peripheral substituent influences the extent of cell photodamage. Moreover, we have demonstrated that PS concentration, physicochemical properties and further light activation determine the PDT response. All porphyrins were clearly localized as a punctuated pattern in the cytoplasm of the cells, and the PDT scheme resulted in apoptotic cell death after 3 h post-PDT. The tri-cationic porphyrin derivatives Tri-Py+–Me–PF, Tri-Py+–Me–Ph and Tri-Py+–Me–CO2Me showed a promising ability, making them good photosensitizer candidates for oncological PDT.
Fil: Prack Mc Cormick, Bárbara Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pansa, Maria Florencia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Milla Sanabria, Laura Natalia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carvalho, Carla Marisa Brito. University of Aveiro; Portugal
Fil: Faustino, Maria Amparo Ferreira. University of Aveiro; Portugal
Fil: Neves, Maria Graça P. M. S.. University of Aveiro; Portugal
Fil: Cavaleiro, José A. S.. University of Aveiro; Portugal
Fil: Vittar, Natalia Belen Rumie. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rivarola, Viviana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Current studies in photodynamic therapy (PDT) against cancer are focused on the development of new photosensitizers (PSs), with higher phototoxic action. The aim of this study was to compare the therapeutic efficiency of tri-cationic meso-substituted porphyrin derivatives (Tri-Py+–Me–PF, Tri-Py+–Me–Ph, Tri-Py+–Me–CO2Me and Tri-Py+–Me–CO2H) with the well-known tetra-cationic T4PM. The phototoxic action of these derivatives was assessed in human colon adenocarcinoma cells by cell viability, intracellular localization and nuclear morphology analysis. In the experimental conditions used we determined that after light activation –PF, –Ph and –CO2Me cause a more significant decline of cell viability compared to –CO2H and T4PM. These results suggest that the nature of the peripheral substituent influences the extent of cell photodamage. Moreover, we have demonstrated that PS concentration, physicochemical properties and further light activation determine the PDT response. All porphyrins were clearly localized as a punctuated pattern in the cytoplasm of the cells, and the PDT scheme resulted in apoptotic cell death after 3 h post-PDT. The tri-cationic porphyrin derivatives Tri-Py+–Me–PF, Tri-Py+–Me–Ph and Tri-Py+–Me–CO2Me showed a promising ability, making them good photosensitizer candidates for oncological PDT.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16854
Prack Mc Cormick, Bárbara Patricia; Pansa, Maria Florencia; Milla Sanabria, Laura Natalia; Carvalho, Carla Marisa Brito; Faustino, Maria Amparo Ferreira; et al.; Cationic porphyrin derivatives for application in photodynamic therapy of cancer; IOP Publishing; Laser Physics; 24; 4; 2-2014; 1-8
1054-660X
url http://hdl.handle.net/11336/16854
identifier_str_mv Prack Mc Cormick, Bárbara Patricia; Pansa, Maria Florencia; Milla Sanabria, Laura Natalia; Carvalho, Carla Marisa Brito; Faustino, Maria Amparo Ferreira; et al.; Cationic porphyrin derivatives for application in photodynamic therapy of cancer; IOP Publishing; Laser Physics; 24; 4; 2-2014; 1-8
1054-660X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1054-660X/24/4/045603/meta;jsessionid=238DA785B6911E74D01395FCCF0DDFF2.ip-10-40-1-105#top
info:eu-repo/semantics/altIdentifier/doi/10.1088/1054-660X/24/4/045603
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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