Cationic porphyrin derivatives for application in photodynamic therapy of cancer
- Autores
- Prack Mc Cormick, Bárbara Patricia; Pansa, Maria Florencia; Milla Sanabria, Laura Natalia; Carvalho, Carla Marisa Brito; Faustino, Maria Amparo Ferreira; Neves, Maria Graça P. M. S.; Cavaleiro, José A. S.; Vittar, Natalia Belen Rumie; Rivarola, Viviana
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Current studies in photodynamic therapy (PDT) against cancer are focused on the development of new photosensitizers (PSs), with higher phototoxic action. The aim of this study was to compare the therapeutic efficiency of tri-cationic meso-substituted porphyrin derivatives (Tri-Py+–Me–PF, Tri-Py+–Me–Ph, Tri-Py+–Me–CO2Me and Tri-Py+–Me–CO2H) with the well-known tetra-cationic T4PM. The phototoxic action of these derivatives was assessed in human colon adenocarcinoma cells by cell viability, intracellular localization and nuclear morphology analysis. In the experimental conditions used we determined that after light activation –PF, –Ph and –CO2Me cause a more significant decline of cell viability compared to –CO2H and T4PM. These results suggest that the nature of the peripheral substituent influences the extent of cell photodamage. Moreover, we have demonstrated that PS concentration, physicochemical properties and further light activation determine the PDT response. All porphyrins were clearly localized as a punctuated pattern in the cytoplasm of the cells, and the PDT scheme resulted in apoptotic cell death after 3 h post-PDT. The tri-cationic porphyrin derivatives Tri-Py+–Me–PF, Tri-Py+–Me–Ph and Tri-Py+–Me–CO2Me showed a promising ability, making them good photosensitizer candidates for oncological PDT.
Fil: Prack Mc Cormick, Bárbara Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pansa, Maria Florencia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Milla Sanabria, Laura Natalia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carvalho, Carla Marisa Brito. University of Aveiro; Portugal
Fil: Faustino, Maria Amparo Ferreira. University of Aveiro; Portugal
Fil: Neves, Maria Graça P. M. S.. University of Aveiro; Portugal
Fil: Cavaleiro, José A. S.. University of Aveiro; Portugal
Fil: Vittar, Natalia Belen Rumie. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rivarola, Viviana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Terapia Fotodinamica
Cancer
Porfirinas
Apoptosis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/16854
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/16854 |
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Cationic porphyrin derivatives for application in photodynamic therapy of cancerPrack Mc Cormick, Bárbara PatriciaPansa, Maria FlorenciaMilla Sanabria, Laura NataliaCarvalho, Carla Marisa BritoFaustino, Maria Amparo FerreiraNeves, Maria Graça P. M. S.Cavaleiro, José A. S.Vittar, Natalia Belen RumieRivarola, VivianaTerapia FotodinamicaCancerPorfirinasApoptosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Current studies in photodynamic therapy (PDT) against cancer are focused on the development of new photosensitizers (PSs), with higher phototoxic action. The aim of this study was to compare the therapeutic efficiency of tri-cationic meso-substituted porphyrin derivatives (Tri-Py+–Me–PF, Tri-Py+–Me–Ph, Tri-Py+–Me–CO2Me and Tri-Py+–Me–CO2H) with the well-known tetra-cationic T4PM. The phototoxic action of these derivatives was assessed in human colon adenocarcinoma cells by cell viability, intracellular localization and nuclear morphology analysis. In the experimental conditions used we determined that after light activation –PF, –Ph and –CO2Me cause a more significant decline of cell viability compared to –CO2H and T4PM. These results suggest that the nature of the peripheral substituent influences the extent of cell photodamage. Moreover, we have demonstrated that PS concentration, physicochemical properties and further light activation determine the PDT response. All porphyrins were clearly localized as a punctuated pattern in the cytoplasm of the cells, and the PDT scheme resulted in apoptotic cell death after 3 h post-PDT. The tri-cationic porphyrin derivatives Tri-Py+–Me–PF, Tri-Py+–Me–Ph and Tri-Py+–Me–CO2Me showed a promising ability, making them good photosensitizer candidates for oncological PDT.Fil: Prack Mc Cormick, Bárbara Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pansa, Maria Florencia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Milla Sanabria, Laura Natalia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carvalho, Carla Marisa Brito. University of Aveiro; PortugalFil: Faustino, Maria Amparo Ferreira. University of Aveiro; PortugalFil: Neves, Maria Graça P. M. S.. University of Aveiro; PortugalFil: Cavaleiro, José A. S.. University of Aveiro; PortugalFil: Vittar, Natalia Belen Rumie. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Rivarola, Viviana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaIOP Publishing2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16854Prack Mc Cormick, Bárbara Patricia; Pansa, Maria Florencia; Milla Sanabria, Laura Natalia; Carvalho, Carla Marisa Brito; Faustino, Maria Amparo Ferreira; et al.; Cationic porphyrin derivatives for application in photodynamic therapy of cancer; IOP Publishing; Laser Physics; 24; 4; 2-2014; 1-81054-660Xenginfo:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1054-660X/24/4/045603/meta;jsessionid=238DA785B6911E74D01395FCCF0DDFF2.ip-10-40-1-105#topinfo:eu-repo/semantics/altIdentifier/doi/10.1088/1054-660X/24/4/045603info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:18Zoai:ri.conicet.gov.ar:11336/16854instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:18.528CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Cationic porphyrin derivatives for application in photodynamic therapy of cancer |
title |
Cationic porphyrin derivatives for application in photodynamic therapy of cancer |
spellingShingle |
Cationic porphyrin derivatives for application in photodynamic therapy of cancer Prack Mc Cormick, Bárbara Patricia Terapia Fotodinamica Cancer Porfirinas Apoptosis |
title_short |
Cationic porphyrin derivatives for application in photodynamic therapy of cancer |
title_full |
Cationic porphyrin derivatives for application in photodynamic therapy of cancer |
title_fullStr |
Cationic porphyrin derivatives for application in photodynamic therapy of cancer |
title_full_unstemmed |
Cationic porphyrin derivatives for application in photodynamic therapy of cancer |
title_sort |
Cationic porphyrin derivatives for application in photodynamic therapy of cancer |
dc.creator.none.fl_str_mv |
Prack Mc Cormick, Bárbara Patricia Pansa, Maria Florencia Milla Sanabria, Laura Natalia Carvalho, Carla Marisa Brito Faustino, Maria Amparo Ferreira Neves, Maria Graça P. M. S. Cavaleiro, José A. S. Vittar, Natalia Belen Rumie Rivarola, Viviana |
author |
Prack Mc Cormick, Bárbara Patricia |
author_facet |
Prack Mc Cormick, Bárbara Patricia Pansa, Maria Florencia Milla Sanabria, Laura Natalia Carvalho, Carla Marisa Brito Faustino, Maria Amparo Ferreira Neves, Maria Graça P. M. S. Cavaleiro, José A. S. Vittar, Natalia Belen Rumie Rivarola, Viviana |
author_role |
author |
author2 |
Pansa, Maria Florencia Milla Sanabria, Laura Natalia Carvalho, Carla Marisa Brito Faustino, Maria Amparo Ferreira Neves, Maria Graça P. M. S. Cavaleiro, José A. S. Vittar, Natalia Belen Rumie Rivarola, Viviana |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Terapia Fotodinamica Cancer Porfirinas Apoptosis |
topic |
Terapia Fotodinamica Cancer Porfirinas Apoptosis |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Current studies in photodynamic therapy (PDT) against cancer are focused on the development of new photosensitizers (PSs), with higher phototoxic action. The aim of this study was to compare the therapeutic efficiency of tri-cationic meso-substituted porphyrin derivatives (Tri-Py+–Me–PF, Tri-Py+–Me–Ph, Tri-Py+–Me–CO2Me and Tri-Py+–Me–CO2H) with the well-known tetra-cationic T4PM. The phototoxic action of these derivatives was assessed in human colon adenocarcinoma cells by cell viability, intracellular localization and nuclear morphology analysis. In the experimental conditions used we determined that after light activation –PF, –Ph and –CO2Me cause a more significant decline of cell viability compared to –CO2H and T4PM. These results suggest that the nature of the peripheral substituent influences the extent of cell photodamage. Moreover, we have demonstrated that PS concentration, physicochemical properties and further light activation determine the PDT response. All porphyrins were clearly localized as a punctuated pattern in the cytoplasm of the cells, and the PDT scheme resulted in apoptotic cell death after 3 h post-PDT. The tri-cationic porphyrin derivatives Tri-Py+–Me–PF, Tri-Py+–Me–Ph and Tri-Py+–Me–CO2Me showed a promising ability, making them good photosensitizer candidates for oncological PDT. Fil: Prack Mc Cormick, Bárbara Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pansa, Maria Florencia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Milla Sanabria, Laura Natalia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carvalho, Carla Marisa Brito. University of Aveiro; Portugal Fil: Faustino, Maria Amparo Ferreira. University of Aveiro; Portugal Fil: Neves, Maria Graça P. M. S.. University of Aveiro; Portugal Fil: Cavaleiro, José A. S.. University of Aveiro; Portugal Fil: Vittar, Natalia Belen Rumie. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina Fil: Rivarola, Viviana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Current studies in photodynamic therapy (PDT) against cancer are focused on the development of new photosensitizers (PSs), with higher phototoxic action. The aim of this study was to compare the therapeutic efficiency of tri-cationic meso-substituted porphyrin derivatives (Tri-Py+–Me–PF, Tri-Py+–Me–Ph, Tri-Py+–Me–CO2Me and Tri-Py+–Me–CO2H) with the well-known tetra-cationic T4PM. The phototoxic action of these derivatives was assessed in human colon adenocarcinoma cells by cell viability, intracellular localization and nuclear morphology analysis. In the experimental conditions used we determined that after light activation –PF, –Ph and –CO2Me cause a more significant decline of cell viability compared to –CO2H and T4PM. These results suggest that the nature of the peripheral substituent influences the extent of cell photodamage. Moreover, we have demonstrated that PS concentration, physicochemical properties and further light activation determine the PDT response. All porphyrins were clearly localized as a punctuated pattern in the cytoplasm of the cells, and the PDT scheme resulted in apoptotic cell death after 3 h post-PDT. The tri-cationic porphyrin derivatives Tri-Py+–Me–PF, Tri-Py+–Me–Ph and Tri-Py+–Me–CO2Me showed a promising ability, making them good photosensitizer candidates for oncological PDT. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/16854 Prack Mc Cormick, Bárbara Patricia; Pansa, Maria Florencia; Milla Sanabria, Laura Natalia; Carvalho, Carla Marisa Brito; Faustino, Maria Amparo Ferreira; et al.; Cationic porphyrin derivatives for application in photodynamic therapy of cancer; IOP Publishing; Laser Physics; 24; 4; 2-2014; 1-8 1054-660X |
url |
http://hdl.handle.net/11336/16854 |
identifier_str_mv |
Prack Mc Cormick, Bárbara Patricia; Pansa, Maria Florencia; Milla Sanabria, Laura Natalia; Carvalho, Carla Marisa Brito; Faustino, Maria Amparo Ferreira; et al.; Cationic porphyrin derivatives for application in photodynamic therapy of cancer; IOP Publishing; Laser Physics; 24; 4; 2-2014; 1-8 1054-660X |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1054-660X/24/4/045603/meta;jsessionid=238DA785B6911E74D01395FCCF0DDFF2.ip-10-40-1-105#top info:eu-repo/semantics/altIdentifier/doi/10.1088/1054-660X/24/4/045603 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
IOP Publishing |
publisher.none.fl_str_mv |
IOP Publishing |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269023012651008 |
score |
13.13397 |