Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy

Autores
Ceretti, Helena; Ponce, Belén; Ramírez, Silvana A.; Montserrat, Javier Marcelo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work deals with the characterization of a phosphorothioate anchoring strategy for aptamer molecules linked to gold, in the context of electrochemical sensors, using adenosine aptamer as model system. Surface density of immobilized phosphorothioate oligonucleotide sequences has been explored for a range of oligonucleotide concentrations (0.055 -55 μM), finding a consequent variation of molecular surface density (3.5×1011 -2.8 ×1013 molecules/cm2). Most suitable aptamer concentration for adenosine recognition was also explored and found to be around 5.5 μM. As proof of concept of phosphorothioate strategy, electrochemical response to adenosine concentration was measured using a ferrocene-labeled oligonucleotide sequence, and phosphorothioate anchoring thermal stability was compared to thiol immobilization. © 2010 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim.
Fil: Ceretti, Helena. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Ponce, Belén. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Ramírez, Silvana A.. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Montserrat, Javier Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Materia
Adenosine
Aptamers
Electrochemical
Gold
Phosphorothioate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53641
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53641
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategyCeretti, HelenaPonce, BelénRamírez, Silvana A.Montserrat, Javier MarceloAdenosineAptamersElectrochemicalGoldPhosphorothioatehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work deals with the characterization of a phosphorothioate anchoring strategy for aptamer molecules linked to gold, in the context of electrochemical sensors, using adenosine aptamer as model system. Surface density of immobilized phosphorothioate oligonucleotide sequences has been explored for a range of oligonucleotide concentrations (0.055 -55 μM), finding a consequent variation of molecular surface density (3.5×1011 -2.8 ×1013 molecules/cm2). Most suitable aptamer concentration for adenosine recognition was also explored and found to be around 5.5 μM. As proof of concept of phosphorothioate strategy, electrochemical response to adenosine concentration was measured using a ferrocene-labeled oligonucleotide sequence, and phosphorothioate anchoring thermal stability was compared to thiol immobilization. © 2010 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim.Fil: Ceretti, Helena. Universidad Nacional de General Sarmiento. Instituto de Ciencias; ArgentinaFil: Ponce, Belén. Universidad Nacional de General Sarmiento. Instituto de Ciencias; ArgentinaFil: Ramírez, Silvana A.. Universidad Nacional de General Sarmiento. Instituto de Ciencias; ArgentinaFil: Montserrat, Javier Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de General Sarmiento. Instituto de Ciencias; ArgentinaWiley VCH Verlag2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53641Ceretti, Helena; Ponce, Belén; Ramírez, Silvana A.; Montserrat, Javier Marcelo; Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy; Wiley VCH Verlag; Electroanalysis; 22; 2; 1-2010; 147-1501040-0397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200900279info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.200900279info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:54:42Zoai:ri.conicet.gov.ar:11336/53641instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:54:42.474CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy
title Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy
spellingShingle Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy
Ceretti, Helena
Adenosine
Aptamers
Electrochemical
Gold
Phosphorothioate
title_short Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy
title_full Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy
title_fullStr Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy
title_full_unstemmed Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy
title_sort Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy
dc.creator.none.fl_str_mv Ceretti, Helena
Ponce, Belén
Ramírez, Silvana A.
Montserrat, Javier Marcelo
author Ceretti, Helena
author_facet Ceretti, Helena
Ponce, Belén
Ramírez, Silvana A.
Montserrat, Javier Marcelo
author_role author
author2 Ponce, Belén
Ramírez, Silvana A.
Montserrat, Javier Marcelo
author2_role author
author
author
dc.subject.none.fl_str_mv Adenosine
Aptamers
Electrochemical
Gold
Phosphorothioate
topic Adenosine
Aptamers
Electrochemical
Gold
Phosphorothioate
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work deals with the characterization of a phosphorothioate anchoring strategy for aptamer molecules linked to gold, in the context of electrochemical sensors, using adenosine aptamer as model system. Surface density of immobilized phosphorothioate oligonucleotide sequences has been explored for a range of oligonucleotide concentrations (0.055 -55 μM), finding a consequent variation of molecular surface density (3.5×1011 -2.8 ×1013 molecules/cm2). Most suitable aptamer concentration for adenosine recognition was also explored and found to be around 5.5 μM. As proof of concept of phosphorothioate strategy, electrochemical response to adenosine concentration was measured using a ferrocene-labeled oligonucleotide sequence, and phosphorothioate anchoring thermal stability was compared to thiol immobilization. © 2010 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim.
Fil: Ceretti, Helena. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Ponce, Belén. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Ramírez, Silvana A.. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Montserrat, Javier Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
description This work deals with the characterization of a phosphorothioate anchoring strategy for aptamer molecules linked to gold, in the context of electrochemical sensors, using adenosine aptamer as model system. Surface density of immobilized phosphorothioate oligonucleotide sequences has been explored for a range of oligonucleotide concentrations (0.055 -55 μM), finding a consequent variation of molecular surface density (3.5×1011 -2.8 ×1013 molecules/cm2). Most suitable aptamer concentration for adenosine recognition was also explored and found to be around 5.5 μM. As proof of concept of phosphorothioate strategy, electrochemical response to adenosine concentration was measured using a ferrocene-labeled oligonucleotide sequence, and phosphorothioate anchoring thermal stability was compared to thiol immobilization. © 2010 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53641
Ceretti, Helena; Ponce, Belén; Ramírez, Silvana A.; Montserrat, Javier Marcelo; Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy; Wiley VCH Verlag; Electroanalysis; 22; 2; 1-2010; 147-150
1040-0397
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53641
identifier_str_mv Ceretti, Helena; Ponce, Belén; Ramírez, Silvana A.; Montserrat, Javier Marcelo; Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy; Wiley VCH Verlag; Electroanalysis; 22; 2; 1-2010; 147-150
1040-0397
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200900279
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.200900279
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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