Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
- Autores
- Ramos, Loyanne C. B.; Rodrigues, Fernando P.; Biazzotto, Juliana C.; de Paula Machado, Sergio; Slep, Leonardo Daniel; Hamblin, Michael R.; da Silva, Roberto S.
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release.
Fil: Ramos, Loyanne C. B.. Universidade de Sao Paulo; Brasil
Fil: Rodrigues, Fernando P.. Universidade de Sao Paulo; Brasil
Fil: Biazzotto, Juliana C.. Universidade de Sao Paulo; Brasil
Fil: de Paula Machado, Sergio. Universidade Federal do Rio de Janeiro; Brasil
Fil: Slep, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hamblin, Michael R.. Harvard Medical School; Estados Unidos
Fil: da Silva, Roberto S.. Harvard Medical School; Estados Unidos. Universidade de Sao Paulo; Brasil - Materia
-
CONJUGATED RUTHENIUM-ANTIBODY COMPLEX
NITRIC OXIDE DELIVERY AGENT
NITROSYL RUTHENIUM COMPLEXES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/89334
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/89334 |
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network_name_str |
CONICET Digital (CONICET) |
spelling |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complexRamos, Loyanne C. B.Rodrigues, Fernando P.Biazzotto, Juliana C.de Paula Machado, SergioSlep, Leonardo DanielHamblin, Michael R.da Silva, Roberto S.CONJUGATED RUTHENIUM-ANTIBODY COMPLEXNITRIC OXIDE DELIVERY AGENTNITROSYL RUTHENIUM COMPLEXEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release.Fil: Ramos, Loyanne C. B.. Universidade de Sao Paulo; BrasilFil: Rodrigues, Fernando P.. Universidade de Sao Paulo; BrasilFil: Biazzotto, Juliana C.. Universidade de Sao Paulo; BrasilFil: de Paula Machado, Sergio. Universidade Federal do Rio de Janeiro; BrasilFil: Slep, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Hamblin, Michael R.. Harvard Medical School; Estados UnidosFil: da Silva, Roberto S.. Harvard Medical School; Estados Unidos. Universidade de Sao Paulo; BrasilSpringer2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89334Ramos, Loyanne C. B.; Rodrigues, Fernando P.; Biazzotto, Juliana C.; de Paula Machado, Sergio; Slep, Leonardo Daniel; et al.; Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex; Springer; Journal of Biological Inorganic Chemistry; 23; 6; 8-2018; 903-9160949-8257CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/29971501/info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00775-018-1589-xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-018-1589-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:46Zoai:ri.conicet.gov.ar:11336/89334instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:46.81CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
title |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
spellingShingle |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex Ramos, Loyanne C. B. CONJUGATED RUTHENIUM-ANTIBODY COMPLEX NITRIC OXIDE DELIVERY AGENT NITROSYL RUTHENIUM COMPLEXES |
title_short |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
title_full |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
title_fullStr |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
title_full_unstemmed |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
title_sort |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
dc.creator.none.fl_str_mv |
Ramos, Loyanne C. B. Rodrigues, Fernando P. Biazzotto, Juliana C. de Paula Machado, Sergio Slep, Leonardo Daniel Hamblin, Michael R. da Silva, Roberto S. |
author |
Ramos, Loyanne C. B. |
author_facet |
Ramos, Loyanne C. B. Rodrigues, Fernando P. Biazzotto, Juliana C. de Paula Machado, Sergio Slep, Leonardo Daniel Hamblin, Michael R. da Silva, Roberto S. |
author_role |
author |
author2 |
Rodrigues, Fernando P. Biazzotto, Juliana C. de Paula Machado, Sergio Slep, Leonardo Daniel Hamblin, Michael R. da Silva, Roberto S. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
CONJUGATED RUTHENIUM-ANTIBODY COMPLEX NITRIC OXIDE DELIVERY AGENT NITROSYL RUTHENIUM COMPLEXES |
topic |
CONJUGATED RUTHENIUM-ANTIBODY COMPLEX NITRIC OXIDE DELIVERY AGENT NITROSYL RUTHENIUM COMPLEXES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release. Fil: Ramos, Loyanne C. B.. Universidade de Sao Paulo; Brasil Fil: Rodrigues, Fernando P.. Universidade de Sao Paulo; Brasil Fil: Biazzotto, Juliana C.. Universidade de Sao Paulo; Brasil Fil: de Paula Machado, Sergio. Universidade Federal do Rio de Janeiro; Brasil Fil: Slep, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina Fil: Hamblin, Michael R.. Harvard Medical School; Estados Unidos Fil: da Silva, Roberto S.. Harvard Medical School; Estados Unidos. Universidade de Sao Paulo; Brasil |
description |
The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/89334 Ramos, Loyanne C. B.; Rodrigues, Fernando P.; Biazzotto, Juliana C.; de Paula Machado, Sergio; Slep, Leonardo Daniel; et al.; Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex; Springer; Journal of Biological Inorganic Chemistry; 23; 6; 8-2018; 903-916 0949-8257 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/89334 |
identifier_str_mv |
Ramos, Loyanne C. B.; Rodrigues, Fernando P.; Biazzotto, Juliana C.; de Paula Machado, Sergio; Slep, Leonardo Daniel; et al.; Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex; Springer; Journal of Biological Inorganic Chemistry; 23; 6; 8-2018; 903-916 0949-8257 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/29971501/ info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00775-018-1589-x info:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-018-1589-x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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score |
13.070432 |