Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex

Autores
Ramos, Loyanne C. B.; Rodrigues, Fernando P.; Biazzotto, Juliana C.; de Paula Machado, Sergio; Slep, Leonardo Daniel; Hamblin, Michael R.; da Silva, Roberto S.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release.
Fil: Ramos, Loyanne C. B.. Universidade de Sao Paulo; Brasil
Fil: Rodrigues, Fernando P.. Universidade de Sao Paulo; Brasil
Fil: Biazzotto, Juliana C.. Universidade de Sao Paulo; Brasil
Fil: de Paula Machado, Sergio. Universidade Federal do Rio de Janeiro; Brasil
Fil: Slep, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hamblin, Michael R.. Harvard Medical School; Estados Unidos
Fil: da Silva, Roberto S.. Harvard Medical School; Estados Unidos. Universidade de Sao Paulo; Brasil
Materia
CONJUGATED RUTHENIUM-ANTIBODY COMPLEX
NITRIC OXIDE DELIVERY AGENT
NITROSYL RUTHENIUM COMPLEXES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89334
Acceder
id CONICETDig_879d8de1dc8203f95d18b25859f1aed1
oai_identifier_str oai:ri.conicet.gov.ar:11336/89334
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complexRamos, Loyanne C. B.Rodrigues, Fernando P.Biazzotto, Juliana C.de Paula Machado, SergioSlep, Leonardo DanielHamblin, Michael R.da Silva, Roberto S.CONJUGATED RUTHENIUM-ANTIBODY COMPLEXNITRIC OXIDE DELIVERY AGENTNITROSYL RUTHENIUM COMPLEXEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release.Fil: Ramos, Loyanne C. B.. Universidade de Sao Paulo; BrasilFil: Rodrigues, Fernando P.. Universidade de Sao Paulo; BrasilFil: Biazzotto, Juliana C.. Universidade de Sao Paulo; BrasilFil: de Paula Machado, Sergio. Universidade Federal do Rio de Janeiro; BrasilFil: Slep, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Hamblin, Michael R.. Harvard Medical School; Estados UnidosFil: da Silva, Roberto S.. Harvard Medical School; Estados Unidos. Universidade de Sao Paulo; BrasilSpringer2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89334Ramos, Loyanne C. B.; Rodrigues, Fernando P.; Biazzotto, Juliana C.; de Paula Machado, Sergio; Slep, Leonardo Daniel; et al.; Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex; Springer; Journal of Biological Inorganic Chemistry; 23; 6; 8-2018; 903-9160949-8257CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/29971501/info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00775-018-1589-xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-018-1589-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:59:21Zoai:ri.conicet.gov.ar:11336/89334instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:59:22.134CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
title Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
spellingShingle Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
Ramos, Loyanne C. B.
CONJUGATED RUTHENIUM-ANTIBODY COMPLEX
NITRIC OXIDE DELIVERY AGENT
NITROSYL RUTHENIUM COMPLEXES
title_short Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
title_full Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
title_fullStr Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
title_full_unstemmed Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
title_sort Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
dc.creator.none.fl_str_mv Ramos, Loyanne C. B.
Rodrigues, Fernando P.
Biazzotto, Juliana C.
de Paula Machado, Sergio
Slep, Leonardo Daniel
Hamblin, Michael R.
da Silva, Roberto S.
author Ramos, Loyanne C. B.
author_facet Ramos, Loyanne C. B.
Rodrigues, Fernando P.
Biazzotto, Juliana C.
de Paula Machado, Sergio
Slep, Leonardo Daniel
Hamblin, Michael R.
da Silva, Roberto S.
author_role author
author2 Rodrigues, Fernando P.
Biazzotto, Juliana C.
de Paula Machado, Sergio
Slep, Leonardo Daniel
Hamblin, Michael R.
da Silva, Roberto S.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CONJUGATED RUTHENIUM-ANTIBODY COMPLEX
NITRIC OXIDE DELIVERY AGENT
NITROSYL RUTHENIUM COMPLEXES
topic CONJUGATED RUTHENIUM-ANTIBODY COMPLEX
NITRIC OXIDE DELIVERY AGENT
NITROSYL RUTHENIUM COMPLEXES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release.
Fil: Ramos, Loyanne C. B.. Universidade de Sao Paulo; Brasil
Fil: Rodrigues, Fernando P.. Universidade de Sao Paulo; Brasil
Fil: Biazzotto, Juliana C.. Universidade de Sao Paulo; Brasil
Fil: de Paula Machado, Sergio. Universidade Federal do Rio de Janeiro; Brasil
Fil: Slep, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hamblin, Michael R.. Harvard Medical School; Estados Unidos
Fil: da Silva, Roberto S.. Harvard Medical School; Estados Unidos. Universidade de Sao Paulo; Brasil
description The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89334
Ramos, Loyanne C. B.; Rodrigues, Fernando P.; Biazzotto, Juliana C.; de Paula Machado, Sergio; Slep, Leonardo Daniel; et al.; Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex; Springer; Journal of Biological Inorganic Chemistry; 23; 6; 8-2018; 903-916
0949-8257
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89334
identifier_str_mv Ramos, Loyanne C. B.; Rodrigues, Fernando P.; Biazzotto, Juliana C.; de Paula Machado, Sergio; Slep, Leonardo Daniel; et al.; Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex; Springer; Journal of Biological Inorganic Chemistry; 23; 6; 8-2018; 903-916
0949-8257
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/29971501/
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00775-018-1589-x
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-018-1589-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782313688465408
score 12.982451

Publicaciones similares