Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.

Autores
Mazziotta, Lucía; Reynoso, Mauricio Alberto; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Zanetti, María Eugenia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phaseolus vulgaris (common bean) is an agronomic important legume crop native to America, where two centres of genetic diversification (GD) are recognised, one in Mesoamerica and the other in the south Andes. Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type‐α, which is predominant in soils of Mesoamerica. It was previously demonstrated that the host nuclear factor NF‐YC1, which is involved in nodule formation and rhizobial infection, contributes to this preferential selection and enhances nodulation in the domesticated accession NAG12 from Mesoamerica. Here, we show that both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type‐α than with a nodC type‐δ strain. Transcripts of NF‐YC1 significantly increased in roots of these accessions 24 h post‐inoculation (hpi) with the nodC type‐α strain. On the other hand, accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type‐δ, which is predominant in Andean soils. However, NF‐YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type‐δ strain, at least at 24 hpi. RNA interference (RNAi)‐mediated gene silencing of NF‐YC1 in the domesticated Andean accession Alubia showed that NF‐YC1 or a closely related member of this family is required for nodule formation and bacterial infection, in agreement with observations in Mesoamerican common beans. Isolation and sequencing of the full‐length ORF of NF‐YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12. Interestingly, overexpression of NF‐YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage. Our findings suggest that transcriptional and functional variation of NF‐YC1 occurs among genetically diverse bean accessions, which might positively or negatively contribute to the fine‐tuning mechanisms that regulate nodule formation in the common bean–R. etli symbiosis.
Fil: Mazziotta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Reynoso, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
Common Bean
Gene Expression
Nitrogen Fixation
Rhizobia
Transcription Factor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95538

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network_name_str CONICET Digital (CONICET)
spelling Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.Mazziotta, LucíaReynoso, Mauricio AlbertoAguilar, Orlando MarioBlanco, Flavio AntonioZanetti, María EugeniaCommon BeanGene ExpressionNitrogen FixationRhizobiaTranscription Factorhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phaseolus vulgaris (common bean) is an agronomic important legume crop native to America, where two centres of genetic diversification (GD) are recognised, one in Mesoamerica and the other in the south Andes. Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type‐α, which is predominant in soils of Mesoamerica. It was previously demonstrated that the host nuclear factor NF‐YC1, which is involved in nodule formation and rhizobial infection, contributes to this preferential selection and enhances nodulation in the domesticated accession NAG12 from Mesoamerica. Here, we show that both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type‐α than with a nodC type‐δ strain. Transcripts of NF‐YC1 significantly increased in roots of these accessions 24 h post‐inoculation (hpi) with the nodC type‐α strain. On the other hand, accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type‐δ, which is predominant in Andean soils. However, NF‐YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type‐δ strain, at least at 24 hpi. RNA interference (RNAi)‐mediated gene silencing of NF‐YC1 in the domesticated Andean accession Alubia showed that NF‐YC1 or a closely related member of this family is required for nodule formation and bacterial infection, in agreement with observations in Mesoamerican common beans. Isolation and sequencing of the full‐length ORF of NF‐YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12. Interestingly, overexpression of NF‐YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage. Our findings suggest that transcriptional and functional variation of NF‐YC1 occurs among genetically diverse bean accessions, which might positively or negatively contribute to the fine‐tuning mechanisms that regulate nodule formation in the common bean–R. etli symbiosis.Fil: Mazziotta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Reynoso, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaWiley Blackwell Publishing, Inc2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95538Mazziotta, Lucía; Reynoso, Mauricio Alberto; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Zanetti, María Eugenia; Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 5; 9-2013; 808-8181435-8603CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1438-8677.2012.00683.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1438-8677.2012.00683.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:15:46Zoai:ri.conicet.gov.ar:11336/95538instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:15:46.281CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.
title Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.
spellingShingle Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.
Mazziotta, Lucía
Common Bean
Gene Expression
Nitrogen Fixation
Rhizobia
Transcription Factor
title_short Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.
title_full Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.
title_fullStr Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.
title_full_unstemmed Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.
title_sort Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.
dc.creator.none.fl_str_mv Mazziotta, Lucía
Reynoso, Mauricio Alberto
Aguilar, Orlando Mario
Blanco, Flavio Antonio
Zanetti, María Eugenia
author Mazziotta, Lucía
author_facet Mazziotta, Lucía
Reynoso, Mauricio Alberto
Aguilar, Orlando Mario
Blanco, Flavio Antonio
Zanetti, María Eugenia
author_role author
author2 Reynoso, Mauricio Alberto
Aguilar, Orlando Mario
Blanco, Flavio Antonio
Zanetti, María Eugenia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Common Bean
Gene Expression
Nitrogen Fixation
Rhizobia
Transcription Factor
topic Common Bean
Gene Expression
Nitrogen Fixation
Rhizobia
Transcription Factor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phaseolus vulgaris (common bean) is an agronomic important legume crop native to America, where two centres of genetic diversification (GD) are recognised, one in Mesoamerica and the other in the south Andes. Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type‐α, which is predominant in soils of Mesoamerica. It was previously demonstrated that the host nuclear factor NF‐YC1, which is involved in nodule formation and rhizobial infection, contributes to this preferential selection and enhances nodulation in the domesticated accession NAG12 from Mesoamerica. Here, we show that both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type‐α than with a nodC type‐δ strain. Transcripts of NF‐YC1 significantly increased in roots of these accessions 24 h post‐inoculation (hpi) with the nodC type‐α strain. On the other hand, accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type‐δ, which is predominant in Andean soils. However, NF‐YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type‐δ strain, at least at 24 hpi. RNA interference (RNAi)‐mediated gene silencing of NF‐YC1 in the domesticated Andean accession Alubia showed that NF‐YC1 or a closely related member of this family is required for nodule formation and bacterial infection, in agreement with observations in Mesoamerican common beans. Isolation and sequencing of the full‐length ORF of NF‐YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12. Interestingly, overexpression of NF‐YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage. Our findings suggest that transcriptional and functional variation of NF‐YC1 occurs among genetically diverse bean accessions, which might positively or negatively contribute to the fine‐tuning mechanisms that regulate nodule formation in the common bean–R. etli symbiosis.
Fil: Mazziotta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Reynoso, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Phaseolus vulgaris (common bean) is an agronomic important legume crop native to America, where two centres of genetic diversification (GD) are recognised, one in Mesoamerica and the other in the south Andes. Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type‐α, which is predominant in soils of Mesoamerica. It was previously demonstrated that the host nuclear factor NF‐YC1, which is involved in nodule formation and rhizobial infection, contributes to this preferential selection and enhances nodulation in the domesticated accession NAG12 from Mesoamerica. Here, we show that both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type‐α than with a nodC type‐δ strain. Transcripts of NF‐YC1 significantly increased in roots of these accessions 24 h post‐inoculation (hpi) with the nodC type‐α strain. On the other hand, accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type‐δ, which is predominant in Andean soils. However, NF‐YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type‐δ strain, at least at 24 hpi. RNA interference (RNAi)‐mediated gene silencing of NF‐YC1 in the domesticated Andean accession Alubia showed that NF‐YC1 or a closely related member of this family is required for nodule formation and bacterial infection, in agreement with observations in Mesoamerican common beans. Isolation and sequencing of the full‐length ORF of NF‐YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12. Interestingly, overexpression of NF‐YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage. Our findings suggest that transcriptional and functional variation of NF‐YC1 occurs among genetically diverse bean accessions, which might positively or negatively contribute to the fine‐tuning mechanisms that regulate nodule formation in the common bean–R. etli symbiosis.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95538
Mazziotta, Lucía; Reynoso, Mauricio Alberto; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Zanetti, María Eugenia; Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 5; 9-2013; 808-818
1435-8603
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95538
identifier_str_mv Mazziotta, Lucía; Reynoso, Mauricio Alberto; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Zanetti, María Eugenia; Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 5; 9-2013; 808-818
1435-8603
CONICET Digital
CONICET
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