Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.
- Autores
- Mazziotta, Lucía; Reynoso, Mauricio Alberto; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Zanetti, María Eugenia
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Phaseolus vulgaris (common bean) is an agronomic important legume crop native to America, where two centres of genetic diversification (GD) are recognised, one in Mesoamerica and the other in the south Andes. Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type‐α, which is predominant in soils of Mesoamerica. It was previously demonstrated that the host nuclear factor NF‐YC1, which is involved in nodule formation and rhizobial infection, contributes to this preferential selection and enhances nodulation in the domesticated accession NAG12 from Mesoamerica. Here, we show that both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type‐α than with a nodC type‐δ strain. Transcripts of NF‐YC1 significantly increased in roots of these accessions 24 h post‐inoculation (hpi) with the nodC type‐α strain. On the other hand, accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type‐δ, which is predominant in Andean soils. However, NF‐YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type‐δ strain, at least at 24 hpi. RNA interference (RNAi)‐mediated gene silencing of NF‐YC1 in the domesticated Andean accession Alubia showed that NF‐YC1 or a closely related member of this family is required for nodule formation and bacterial infection, in agreement with observations in Mesoamerican common beans. Isolation and sequencing of the full‐length ORF of NF‐YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12. Interestingly, overexpression of NF‐YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage. Our findings suggest that transcriptional and functional variation of NF‐YC1 occurs among genetically diverse bean accessions, which might positively or negatively contribute to the fine‐tuning mechanisms that regulate nodule formation in the common bean–R. etli symbiosis.
Fil: Mazziotta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Reynoso, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina - Materia
-
Common Bean
Gene Expression
Nitrogen Fixation
Rhizobia
Transcription Factor - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/95538
Ver los metadatos del registro completo
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spelling |
Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.Mazziotta, LucíaReynoso, Mauricio AlbertoAguilar, Orlando MarioBlanco, Flavio AntonioZanetti, María EugeniaCommon BeanGene ExpressionNitrogen FixationRhizobiaTranscription Factorhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phaseolus vulgaris (common bean) is an agronomic important legume crop native to America, where two centres of genetic diversification (GD) are recognised, one in Mesoamerica and the other in the south Andes. Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type‐α, which is predominant in soils of Mesoamerica. It was previously demonstrated that the host nuclear factor NF‐YC1, which is involved in nodule formation and rhizobial infection, contributes to this preferential selection and enhances nodulation in the domesticated accession NAG12 from Mesoamerica. Here, we show that both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type‐α than with a nodC type‐δ strain. Transcripts of NF‐YC1 significantly increased in roots of these accessions 24 h post‐inoculation (hpi) with the nodC type‐α strain. On the other hand, accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type‐δ, which is predominant in Andean soils. However, NF‐YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type‐δ strain, at least at 24 hpi. RNA interference (RNAi)‐mediated gene silencing of NF‐YC1 in the domesticated Andean accession Alubia showed that NF‐YC1 or a closely related member of this family is required for nodule formation and bacterial infection, in agreement with observations in Mesoamerican common beans. Isolation and sequencing of the full‐length ORF of NF‐YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12. Interestingly, overexpression of NF‐YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage. Our findings suggest that transcriptional and functional variation of NF‐YC1 occurs among genetically diverse bean accessions, which might positively or negatively contribute to the fine‐tuning mechanisms that regulate nodule formation in the common bean–R. etli symbiosis.Fil: Mazziotta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Reynoso, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaWiley Blackwell Publishing, Inc2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95538Mazziotta, Lucía; Reynoso, Mauricio Alberto; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Zanetti, María Eugenia; Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 5; 9-2013; 808-8181435-8603CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1438-8677.2012.00683.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1438-8677.2012.00683.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:15:46Zoai:ri.conicet.gov.ar:11336/95538instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:15:46.281CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli. |
title |
Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli. |
spellingShingle |
Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli. Mazziotta, Lucía Common Bean Gene Expression Nitrogen Fixation Rhizobia Transcription Factor |
title_short |
Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli. |
title_full |
Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli. |
title_fullStr |
Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli. |
title_full_unstemmed |
Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli. |
title_sort |
Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli. |
dc.creator.none.fl_str_mv |
Mazziotta, Lucía Reynoso, Mauricio Alberto Aguilar, Orlando Mario Blanco, Flavio Antonio Zanetti, María Eugenia |
author |
Mazziotta, Lucía |
author_facet |
Mazziotta, Lucía Reynoso, Mauricio Alberto Aguilar, Orlando Mario Blanco, Flavio Antonio Zanetti, María Eugenia |
author_role |
author |
author2 |
Reynoso, Mauricio Alberto Aguilar, Orlando Mario Blanco, Flavio Antonio Zanetti, María Eugenia |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Common Bean Gene Expression Nitrogen Fixation Rhizobia Transcription Factor |
topic |
Common Bean Gene Expression Nitrogen Fixation Rhizobia Transcription Factor |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Phaseolus vulgaris (common bean) is an agronomic important legume crop native to America, where two centres of genetic diversification (GD) are recognised, one in Mesoamerica and the other in the south Andes. Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type‐α, which is predominant in soils of Mesoamerica. It was previously demonstrated that the host nuclear factor NF‐YC1, which is involved in nodule formation and rhizobial infection, contributes to this preferential selection and enhances nodulation in the domesticated accession NAG12 from Mesoamerica. Here, we show that both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type‐α than with a nodC type‐δ strain. Transcripts of NF‐YC1 significantly increased in roots of these accessions 24 h post‐inoculation (hpi) with the nodC type‐α strain. On the other hand, accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type‐δ, which is predominant in Andean soils. However, NF‐YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type‐δ strain, at least at 24 hpi. RNA interference (RNAi)‐mediated gene silencing of NF‐YC1 in the domesticated Andean accession Alubia showed that NF‐YC1 or a closely related member of this family is required for nodule formation and bacterial infection, in agreement with observations in Mesoamerican common beans. Isolation and sequencing of the full‐length ORF of NF‐YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12. Interestingly, overexpression of NF‐YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage. Our findings suggest that transcriptional and functional variation of NF‐YC1 occurs among genetically diverse bean accessions, which might positively or negatively contribute to the fine‐tuning mechanisms that regulate nodule formation in the common bean–R. etli symbiosis. Fil: Mazziotta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Reynoso, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina |
description |
Phaseolus vulgaris (common bean) is an agronomic important legume crop native to America, where two centres of genetic diversification (GD) are recognised, one in Mesoamerica and the other in the south Andes. Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type‐α, which is predominant in soils of Mesoamerica. It was previously demonstrated that the host nuclear factor NF‐YC1, which is involved in nodule formation and rhizobial infection, contributes to this preferential selection and enhances nodulation in the domesticated accession NAG12 from Mesoamerica. Here, we show that both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type‐α than with a nodC type‐δ strain. Transcripts of NF‐YC1 significantly increased in roots of these accessions 24 h post‐inoculation (hpi) with the nodC type‐α strain. On the other hand, accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type‐δ, which is predominant in Andean soils. However, NF‐YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type‐δ strain, at least at 24 hpi. RNA interference (RNAi)‐mediated gene silencing of NF‐YC1 in the domesticated Andean accession Alubia showed that NF‐YC1 or a closely related member of this family is required for nodule formation and bacterial infection, in agreement with observations in Mesoamerican common beans. Isolation and sequencing of the full‐length ORF of NF‐YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12. Interestingly, overexpression of NF‐YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage. Our findings suggest that transcriptional and functional variation of NF‐YC1 occurs among genetically diverse bean accessions, which might positively or negatively contribute to the fine‐tuning mechanisms that regulate nodule formation in the common bean–R. etli symbiosis. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/95538 Mazziotta, Lucía; Reynoso, Mauricio Alberto; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Zanetti, María Eugenia; Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 5; 9-2013; 808-818 1435-8603 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/95538 |
identifier_str_mv |
Mazziotta, Lucía; Reynoso, Mauricio Alberto; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Zanetti, María Eugenia; Transcriptional and functional variation of NF-YC1 in genetically diverse accessions of P. vulgaris during the symbiotic association with Rhizobium etli.; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 5; 9-2013; 808-818 1435-8603 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1438-8677.2012.00683.x info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1438-8677.2012.00683.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083305642917888 |
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13.22299 |