The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme

Autores
Fernandino, Juan Ignacio; Hattori, Ricardo Shohei; Kishii, Ai; Strüssmann, Carlos Augusto; Somoza, Gustavo Manuel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In many ectotherm species the gonadal fate is modulated by temperature early in life (Temperature-Dependent Sex Determination, TSD) but the transducer mechanism between temperature and gonadal differentiation is still elusive. We have recently shown that cortisol, the glucocorticoid stress-related hormone in vertebrates, is involved in the TSD process of pejerrey, Odontesthes bonariensis. Particularly, all larvae exposed to a male-producing temperature (MPT, 29°C) after hatching showed increased whole-body cortisol and 11-ketotestosterone (11-KT, the main bioactive androgen in fish) levels and developed as males. Moreover, cortisol administration at an intermediate, mixed sex-producing temperature (MixPT, 24°C) caused increases in 11-KT and in the frequency of males, suggesting a relation between this glucocorticoid and androgens during the masculinization process. In order to clarify the link between stress and masculinization, the expression of hsd11b2, glucocorticoid receptors gr1 and gr2, and androgen receptors ar1 and ar2 was analyzed by RT-qPCR and in situ hybridization in larvae reared at MPT, MixPT, and FPT (female-producing temperature, 17°C) during the sex determination period. We also analyzed the effects of cortisol treatment in larvae reared at MixPT and in adult testicular explants incubated in vitro. MPT and cortisol treatment produced significant increases in hsd11b2 mRNA expression. Also, gonadal explants incubated in the presence of cortisol showed increases of 11-KT levels in the medium. Taken together these results suggest that cortisol promotes 11-KT production during high temperature-induced masculinization by modulation of hsd11b2 expression and thus drives the morphogenesis of the testes.
Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Hattori, Ricardo Shohei. University of Marine Science and Technology; Japón
Fil: Kishii, Ai. University of Marine Science and Technology; Japón
Fil: Strüssmann, Carlos Augusto. University of Marine Science and Technology; Japón
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Materia
11-BHSD2
cortisol
masculinization
pejerrey
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/270074
Acceder
id CONICETDig_869003c27597bb3dcc8ba79f98db69f1
oai_identifier_str oai:ri.conicet.gov.ar:11336/270074
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key EnzymeFernandino, Juan IgnacioHattori, Ricardo ShoheiKishii, AiStrüssmann, Carlos AugustoSomoza, Gustavo Manuel11-BHSD2cortisolmasculinizationpejerreyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In many ectotherm species the gonadal fate is modulated by temperature early in life (Temperature-Dependent Sex Determination, TSD) but the transducer mechanism between temperature and gonadal differentiation is still elusive. We have recently shown that cortisol, the glucocorticoid stress-related hormone in vertebrates, is involved in the TSD process of pejerrey, Odontesthes bonariensis. Particularly, all larvae exposed to a male-producing temperature (MPT, 29°C) after hatching showed increased whole-body cortisol and 11-ketotestosterone (11-KT, the main bioactive androgen in fish) levels and developed as males. Moreover, cortisol administration at an intermediate, mixed sex-producing temperature (MixPT, 24°C) caused increases in 11-KT and in the frequency of males, suggesting a relation between this glucocorticoid and androgens during the masculinization process. In order to clarify the link between stress and masculinization, the expression of hsd11b2, glucocorticoid receptors gr1 and gr2, and androgen receptors ar1 and ar2 was analyzed by RT-qPCR and in situ hybridization in larvae reared at MPT, MixPT, and FPT (female-producing temperature, 17°C) during the sex determination period. We also analyzed the effects of cortisol treatment in larvae reared at MixPT and in adult testicular explants incubated in vitro. MPT and cortisol treatment produced significant increases in hsd11b2 mRNA expression. Also, gonadal explants incubated in the presence of cortisol showed increases of 11-KT levels in the medium. Taken together these results suggest that cortisol promotes 11-KT production during high temperature-induced masculinization by modulation of hsd11b2 expression and thus drives the morphogenesis of the testes.Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Hattori, Ricardo Shohei. University of Marine Science and Technology; JapónFil: Kishii, Ai. University of Marine Science and Technology; JapónFil: Strüssmann, Carlos Augusto. University of Marine Science and Technology; JapónFil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaEndocrine Society2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/270074Fernandino, Juan Ignacio; Hattori, Ricardo Shohei; Kishii, Ai; Strüssmann, Carlos Augusto; Somoza, Gustavo Manuel; The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme; Endocrine Society; Endocrinology; 153; 12; 12-2012; 6003-60110013-7227CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1210/en.2012-1517info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:52:36Zoai:ri.conicet.gov.ar:11336/270074instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:52:36.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme
title The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme
spellingShingle The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme
Fernandino, Juan Ignacio
11-BHSD2
cortisol
masculinization
pejerrey
title_short The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme
title_full The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme
title_fullStr The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme
title_full_unstemmed The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme
title_sort The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme
dc.creator.none.fl_str_mv Fernandino, Juan Ignacio
Hattori, Ricardo Shohei
Kishii, Ai
Strüssmann, Carlos Augusto
Somoza, Gustavo Manuel
author Fernandino, Juan Ignacio
author_facet Fernandino, Juan Ignacio
Hattori, Ricardo Shohei
Kishii, Ai
Strüssmann, Carlos Augusto
Somoza, Gustavo Manuel
author_role author
author2 Hattori, Ricardo Shohei
Kishii, Ai
Strüssmann, Carlos Augusto
Somoza, Gustavo Manuel
author2_role author
author
author
author
dc.subject.none.fl_str_mv 11-BHSD2
cortisol
masculinization
pejerrey
topic 11-BHSD2
cortisol
masculinization
pejerrey
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In many ectotherm species the gonadal fate is modulated by temperature early in life (Temperature-Dependent Sex Determination, TSD) but the transducer mechanism between temperature and gonadal differentiation is still elusive. We have recently shown that cortisol, the glucocorticoid stress-related hormone in vertebrates, is involved in the TSD process of pejerrey, Odontesthes bonariensis. Particularly, all larvae exposed to a male-producing temperature (MPT, 29°C) after hatching showed increased whole-body cortisol and 11-ketotestosterone (11-KT, the main bioactive androgen in fish) levels and developed as males. Moreover, cortisol administration at an intermediate, mixed sex-producing temperature (MixPT, 24°C) caused increases in 11-KT and in the frequency of males, suggesting a relation between this glucocorticoid and androgens during the masculinization process. In order to clarify the link between stress and masculinization, the expression of hsd11b2, glucocorticoid receptors gr1 and gr2, and androgen receptors ar1 and ar2 was analyzed by RT-qPCR and in situ hybridization in larvae reared at MPT, MixPT, and FPT (female-producing temperature, 17°C) during the sex determination period. We also analyzed the effects of cortisol treatment in larvae reared at MixPT and in adult testicular explants incubated in vitro. MPT and cortisol treatment produced significant increases in hsd11b2 mRNA expression. Also, gonadal explants incubated in the presence of cortisol showed increases of 11-KT levels in the medium. Taken together these results suggest that cortisol promotes 11-KT production during high temperature-induced masculinization by modulation of hsd11b2 expression and thus drives the morphogenesis of the testes.
Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Hattori, Ricardo Shohei. University of Marine Science and Technology; Japón
Fil: Kishii, Ai. University of Marine Science and Technology; Japón
Fil: Strüssmann, Carlos Augusto. University of Marine Science and Technology; Japón
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
description In many ectotherm species the gonadal fate is modulated by temperature early in life (Temperature-Dependent Sex Determination, TSD) but the transducer mechanism between temperature and gonadal differentiation is still elusive. We have recently shown that cortisol, the glucocorticoid stress-related hormone in vertebrates, is involved in the TSD process of pejerrey, Odontesthes bonariensis. Particularly, all larvae exposed to a male-producing temperature (MPT, 29°C) after hatching showed increased whole-body cortisol and 11-ketotestosterone (11-KT, the main bioactive androgen in fish) levels and developed as males. Moreover, cortisol administration at an intermediate, mixed sex-producing temperature (MixPT, 24°C) caused increases in 11-KT and in the frequency of males, suggesting a relation between this glucocorticoid and androgens during the masculinization process. In order to clarify the link between stress and masculinization, the expression of hsd11b2, glucocorticoid receptors gr1 and gr2, and androgen receptors ar1 and ar2 was analyzed by RT-qPCR and in situ hybridization in larvae reared at MPT, MixPT, and FPT (female-producing temperature, 17°C) during the sex determination period. We also analyzed the effects of cortisol treatment in larvae reared at MixPT and in adult testicular explants incubated in vitro. MPT and cortisol treatment produced significant increases in hsd11b2 mRNA expression. Also, gonadal explants incubated in the presence of cortisol showed increases of 11-KT levels in the medium. Taken together these results suggest that cortisol promotes 11-KT production during high temperature-induced masculinization by modulation of hsd11b2 expression and thus drives the morphogenesis of the testes.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/270074
Fernandino, Juan Ignacio; Hattori, Ricardo Shohei; Kishii, Ai; Strüssmann, Carlos Augusto; Somoza, Gustavo Manuel; The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme; Endocrine Society; Endocrinology; 153; 12; 12-2012; 6003-6011
0013-7227
CONICET Digital
CONICET
url http://hdl.handle.net/11336/270074
identifier_str_mv Fernandino, Juan Ignacio; Hattori, Ricardo Shohei; Kishii, Ai; Strüssmann, Carlos Augusto; Somoza, Gustavo Manuel; The Cortisol and Androgen Pathways Cross Talk in High Temperature-Induced Masculinization: The 11β-Hydroxysteroid Dehydrogenase as a Key Enzyme; Endocrine Society; Endocrinology; 153; 12; 12-2012; 6003-6011
0013-7227
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2012-1517
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Endocrine Society
publisher.none.fl_str_mv Endocrine Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083054813052928
score 13.22299

Publicaciones similares