Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate
- Autores
- Mattos Martins, Marina Camara; Hejazi, Mahdi; Fettke, Joerg; Steup, Martin; Feil, Regina; Krause, Ursula; Arrivault, Stéphanie; Vosloh, Daniel; Figueroa, Carlos Maria; Ivakov, Alexander; Yadav, Umesh Prasad; Piques, Maria; Metzner, Daniela; Stitt, Mark; Lunn, John Edward
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Many plants accumulate substantial starch reserves in their leaves during the day and remobilize them at night to provide carbon and energy for maintenance and growth. In this paper, we explore the role of a sugar-signaling metabolite, trehalose-6-phosphate (Tre6P), in regulating the accumulation and turnover of transitory starch in Arabidopsis (Arabidopsis thaliana) leaves. Ethanol-induced overexpression of trehalose-phosphate synthase during the day increased Tre6P levels up to 11-fold. There was a transient increase in the rate of starch accumulation in the middle of the day, but this was not linked to reductive activation of ADP-glucose pyrophosphorylase. A 2- to 3-fold increase in Tre6P during the night led to significant inhibition of starch degradation. Maltose and maltotriose did not accumulate, suggesting that Tre6P affects an early step in the pathway of starch degradation in the chloroplasts. Starch granules isolated from induced plants had a higher orthophosphate content than granules from noninduced control plants, consistent either with disruption of the phosphorylation-dephosphorylation cycle that is essential for efficient starch breakdown or with inhibition of starch hydrolysis by beta-amylase. Nonaqueous fractionation of leaves showed that Tre6P is predominantly located in the cytosol, with estimated in vivo Tre6P concentrations of 4 to 7 µM in the cytosol, 0.2 to 0.5 µM in the chloroplasts, and 0.05 µM in the vacuole. It is proposed that Tre6P is a component in a signaling pathway that mediates the feedback regulation of starch breakdown by sucrose, potentially linking starch turnover to demand for sucrose by growing sink organs at night.
Fil: Mattos Martins, Marina Camara. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Hejazi, Mahdi. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Fettke, Joerg. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Steup, Martin. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Krause, Ursula. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Arrivault, Stéphanie. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Vosloh, Daniel. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Ivakov, Alexander. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Yadav, Umesh Prasad. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Piques, Maria. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Metzner, Daniela. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Lunn, John Edward. Max Planck Institute of Molecular Plant Physiology; Alemania - Materia
- TREHALOSE-6-PHOSPHATE
- Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/27531
Ver los metadatos del registro completo
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Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphateMattos Martins, Marina CamaraHejazi, MahdiFettke, JoergSteup, MartinFeil, ReginaKrause, UrsulaArrivault, StéphanieVosloh, DanielFigueroa, Carlos MariaIvakov, AlexanderYadav, Umesh PrasadPiques, MariaMetzner, DanielaStitt, MarkLunn, John EdwardTREHALOSE-6-PHOSPHATEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Many plants accumulate substantial starch reserves in their leaves during the day and remobilize them at night to provide carbon and energy for maintenance and growth. In this paper, we explore the role of a sugar-signaling metabolite, trehalose-6-phosphate (Tre6P), in regulating the accumulation and turnover of transitory starch in Arabidopsis (<em>Arabidopsis thaliana</em>) leaves. Ethanol-induced overexpression of trehalose-phosphate synthase during the day increased Tre6P levels up to 11-fold. There was a transient increase in the rate of starch accumulation in the middle of the day, but this was not linked to reductive activation of ADP-glucose pyrophosphorylase. A 2- to 3-fold increase in Tre6P during the night led to significant inhibition of starch degradation. Maltose and maltotriose did not accumulate, suggesting that Tre6P affects an early step in the pathway of starch degradation in the chloroplasts. Starch granules isolated from induced plants had a higher orthophosphate content than granules from noninduced control plants, consistent either with disruption of the phosphorylation-dephosphorylation cycle that is essential for efficient starch breakdown or with inhibition of starch hydrolysis by beta-amylase. Nonaqueous fractionation of leaves showed that Tre6P is predominantly located in the cytosol, with estimated in vivo Tre6P concentrations of 4 to 7 µM in the cytosol, 0.2 to 0.5 µM in the chloroplasts, and 0.05 µM in the vacuole. It is proposed that Tre6P is a component in a signaling pathway that mediates the feedback regulation of starch breakdown by sucrose, potentially linking starch turnover to demand for sucrose by growing sink organs at night.Fil: Mattos Martins, Marina Camara. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Hejazi, Mahdi. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Fettke, Joerg. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Steup, Martin. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Krause, Ursula. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Arrivault, Stéphanie. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Vosloh, Daniel. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Ivakov, Alexander. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Yadav, Umesh Prasad. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Piques, Maria. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Metzner, Daniela. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Lunn, John Edward. Max Planck Institute of Molecular Plant Physiology; AlemaniaAmerican Society of Plant Biologist2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27531Mattos Martins, Marina Camara; Hejazi, Mahdi; Fettke, Joerg; Steup, Martin; Feil, Regina; et al.; Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate; American Society of Plant Biologist; Plant Physiology; 163; 3; 11-2013; 1142-11630032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/163/3/1142info:eu-repo/semantics/altIdentifier/doi/10.1104%2Fpp.113.226787info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:41Zoai:ri.conicet.gov.ar:11336/27531instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:42.085CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate |
| title |
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate |
| spellingShingle |
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate Mattos Martins, Marina Camara TREHALOSE-6-PHOSPHATE |
| title_short |
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate |
| title_full |
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate |
| title_fullStr |
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate |
| title_full_unstemmed |
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate |
| title_sort |
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate |
| dc.creator.none.fl_str_mv |
Mattos Martins, Marina Camara Hejazi, Mahdi Fettke, Joerg Steup, Martin Feil, Regina Krause, Ursula Arrivault, Stéphanie Vosloh, Daniel Figueroa, Carlos Maria Ivakov, Alexander Yadav, Umesh Prasad Piques, Maria Metzner, Daniela Stitt, Mark Lunn, John Edward |
| author |
Mattos Martins, Marina Camara |
| author_facet |
Mattos Martins, Marina Camara Hejazi, Mahdi Fettke, Joerg Steup, Martin Feil, Regina Krause, Ursula Arrivault, Stéphanie Vosloh, Daniel Figueroa, Carlos Maria Ivakov, Alexander Yadav, Umesh Prasad Piques, Maria Metzner, Daniela Stitt, Mark Lunn, John Edward |
| author_role |
author |
| author2 |
Hejazi, Mahdi Fettke, Joerg Steup, Martin Feil, Regina Krause, Ursula Arrivault, Stéphanie Vosloh, Daniel Figueroa, Carlos Maria Ivakov, Alexander Yadav, Umesh Prasad Piques, Maria Metzner, Daniela Stitt, Mark Lunn, John Edward |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
TREHALOSE-6-PHOSPHATE |
| topic |
TREHALOSE-6-PHOSPHATE |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Many plants accumulate substantial starch reserves in their leaves during the day and remobilize them at night to provide carbon and energy for maintenance and growth. In this paper, we explore the role of a sugar-signaling metabolite, trehalose-6-phosphate (Tre6P), in regulating the accumulation and turnover of transitory starch in Arabidopsis (<em>Arabidopsis thaliana</em>) leaves. Ethanol-induced overexpression of trehalose-phosphate synthase during the day increased Tre6P levels up to 11-fold. There was a transient increase in the rate of starch accumulation in the middle of the day, but this was not linked to reductive activation of ADP-glucose pyrophosphorylase. A 2- to 3-fold increase in Tre6P during the night led to significant inhibition of starch degradation. Maltose and maltotriose did not accumulate, suggesting that Tre6P affects an early step in the pathway of starch degradation in the chloroplasts. Starch granules isolated from induced plants had a higher orthophosphate content than granules from noninduced control plants, consistent either with disruption of the phosphorylation-dephosphorylation cycle that is essential for efficient starch breakdown or with inhibition of starch hydrolysis by beta-amylase. Nonaqueous fractionation of leaves showed that Tre6P is predominantly located in the cytosol, with estimated in vivo Tre6P concentrations of 4 to 7 µM in the cytosol, 0.2 to 0.5 µM in the chloroplasts, and 0.05 µM in the vacuole. It is proposed that Tre6P is a component in a signaling pathway that mediates the feedback regulation of starch breakdown by sucrose, potentially linking starch turnover to demand for sucrose by growing sink organs at night. Fil: Mattos Martins, Marina Camara. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Hejazi, Mahdi. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Fettke, Joerg. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Steup, Martin. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Krause, Ursula. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Arrivault, Stéphanie. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Vosloh, Daniel. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Ivakov, Alexander. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Yadav, Umesh Prasad. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Piques, Maria. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Metzner, Daniela. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Lunn, John Edward. Max Planck Institute of Molecular Plant Physiology; Alemania |
| description |
Many plants accumulate substantial starch reserves in their leaves during the day and remobilize them at night to provide carbon and energy for maintenance and growth. In this paper, we explore the role of a sugar-signaling metabolite, trehalose-6-phosphate (Tre6P), in regulating the accumulation and turnover of transitory starch in Arabidopsis (<em>Arabidopsis thaliana</em>) leaves. Ethanol-induced overexpression of trehalose-phosphate synthase during the day increased Tre6P levels up to 11-fold. There was a transient increase in the rate of starch accumulation in the middle of the day, but this was not linked to reductive activation of ADP-glucose pyrophosphorylase. A 2- to 3-fold increase in Tre6P during the night led to significant inhibition of starch degradation. Maltose and maltotriose did not accumulate, suggesting that Tre6P affects an early step in the pathway of starch degradation in the chloroplasts. Starch granules isolated from induced plants had a higher orthophosphate content than granules from noninduced control plants, consistent either with disruption of the phosphorylation-dephosphorylation cycle that is essential for efficient starch breakdown or with inhibition of starch hydrolysis by beta-amylase. Nonaqueous fractionation of leaves showed that Tre6P is predominantly located in the cytosol, with estimated in vivo Tre6P concentrations of 4 to 7 µM in the cytosol, 0.2 to 0.5 µM in the chloroplasts, and 0.05 µM in the vacuole. It is proposed that Tre6P is a component in a signaling pathway that mediates the feedback regulation of starch breakdown by sucrose, potentially linking starch turnover to demand for sucrose by growing sink organs at night. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-11 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/27531 Mattos Martins, Marina Camara; Hejazi, Mahdi; Fettke, Joerg; Steup, Martin; Feil, Regina; et al.; Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate; American Society of Plant Biologist; Plant Physiology; 163; 3; 11-2013; 1142-1163 0032-0889 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/27531 |
| identifier_str_mv |
Mattos Martins, Marina Camara; Hejazi, Mahdi; Fettke, Joerg; Steup, Martin; Feil, Regina; et al.; Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate; American Society of Plant Biologist; Plant Physiology; 163; 3; 11-2013; 1142-1163 0032-0889 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/163/3/1142 info:eu-repo/semantics/altIdentifier/doi/10.1104%2Fpp.113.226787 |
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openAccess |
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application/pdf application/pdf |
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American Society of Plant Biologist |
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American Society of Plant Biologist |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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