Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents

Autores
Naso, Luciana Gissella; Lezama, Luis Raul; Rojo, Teófilo; Etcheverry, Susana Beatriz; Valcarcel, Maria; Roura, Meritxell; Salado, Clarisa; Ferrer, Evelina Gloria; Williams, Patricia Ana María
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is known that flavonoids possess, among others, antioxidant and antitumoral properties that depend on their molecular structure. The central objective if this study was to investigate the potential antioxidant and antiproliferative properties of the flavonol morin and its new oxovanadium(IV) complex (VOmor) that was synthesized in order to modify the morin chemical structure. Two osteoblast (UMR106 and MC3T3E1), two breast tumor (T47D and SKBR3) and breast epithelial cell lines in culture were used for the antitumoral determinations. Additionally, a comparative study of their antioxidant capacities using different radicals (DPPH, ABTS+, OH, O2−, ROO) was performed. Selected mechanisms of action were studied using the breast cancer cell lines. Results obtained show that morin and its complex behaved as good antioxidant agents for some of the radicals and that the complexation improved the behavior with respect to OH and O2− radicals being morin more effective as ROO scavenger. A considerable variation in sensitivity was observed in the breast cancer cells but non-specificity was found for the treatment of osteosarcoma. Moreover, the compounds did not affect the normal proliferation of the breast epithelial mammal cells. The mechanistic studies demonstrated that the complex did not generate reactive oxygen species in the cells (confirming the in vitro studies) and did not produce any damage of DNA. The plasmatic membrane was observed to be damaged only in the SKBR3 cell line. In contrast, the perturbation of the mitochondrial membrane potential and the activation of caspase 3/7 for the breast tumor cells revealed an apoptotic cell death process. All these results collectively suggested that VOmor complex could serve as promising pharmacologically active substance against breast cancer treatment.
Fil: Naso, Luciana Gissella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Lezama, Luis Raul. Universidad del País Vasco; España
Fil: Rojo, Teófilo. Universidad del País Vasco; España
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Valcarcel, Maria. Innoprot SL; España
Fil: Roura, Meritxell. Innoprot SL; España
Fil: Salado, Clarisa. Innoprot SL; España
Fil: Ferrer, Evelina Gloria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Williams, Patricia Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Materia
Natural Antioxidant Morin
Oxovanadium(Iv) Complex
Radical Scavengers
Tumor Specificity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24496
Acceder
id CONICETDig_84e221d7adf8338f9db0be7e9f84f9df
oai_identifier_str oai:ri.conicet.gov.ar:11336/24496
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agentsNaso, Luciana GissellaLezama, Luis RaulRojo, TeófiloEtcheverry, Susana BeatrizValcarcel, MariaRoura, MeritxellSalado, ClarisaFerrer, Evelina GloriaWilliams, Patricia Ana MaríaNatural Antioxidant MorinOxovanadium(Iv) ComplexRadical ScavengersTumor Specificityhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1It is known that flavonoids possess, among others, antioxidant and antitumoral properties that depend on their molecular structure. The central objective if this study was to investigate the potential antioxidant and antiproliferative properties of the flavonol morin and its new oxovanadium(IV) complex (VOmor) that was synthesized in order to modify the morin chemical structure. Two osteoblast (UMR106 and MC3T3E1), two breast tumor (T47D and SKBR3) and breast epithelial cell lines in culture were used for the antitumoral determinations. Additionally, a comparative study of their antioxidant capacities using different radicals (DPPH, ABTS+, OH, O2−, ROO) was performed. Selected mechanisms of action were studied using the breast cancer cell lines. Results obtained show that morin and its complex behaved as good antioxidant agents for some of the radicals and that the complexation improved the behavior with respect to OH and O2− radicals being morin more effective as ROO scavenger. A considerable variation in sensitivity was observed in the breast cancer cells but non-specificity was found for the treatment of osteosarcoma. Moreover, the compounds did not affect the normal proliferation of the breast epithelial mammal cells. The mechanistic studies demonstrated that the complex did not generate reactive oxygen species in the cells (confirming the in vitro studies) and did not produce any damage of DNA. The plasmatic membrane was observed to be damaged only in the SKBR3 cell line. In contrast, the perturbation of the mitochondrial membrane potential and the activation of caspase 3/7 for the breast tumor cells revealed an apoptotic cell death process. All these results collectively suggested that VOmor complex could serve as promising pharmacologically active substance against breast cancer treatment.Fil: Naso, Luciana Gissella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Lezama, Luis Raul. Universidad del País Vasco; EspañaFil: Rojo, Teófilo. Universidad del País Vasco; EspañaFil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biológicas. Cátedra Bioquímica Patologica; ArgentinaFil: Valcarcel, Maria. Innoprot SL; EspañaFil: Roura, Meritxell. Innoprot SL; EspañaFil: Salado, Clarisa. Innoprot SL; EspañaFil: Ferrer, Evelina Gloria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Williams, Patricia Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaElsevier Science2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24496Naso, Luciana Gissella; Lezama, Luis Raul; Rojo, Teófilo; Etcheverry, Susana Beatriz; Valcarcel, Maria; et al.; Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents; Elsevier Science; Chemico-biological Interactions; 206; 2; 10-2013; 289-3010009-2797CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbi.2013.10.006info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0009279713002603info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:20:46Zoai:ri.conicet.gov.ar:11336/24496instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:20:46.237CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents
title Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents
spellingShingle Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents
Naso, Luciana Gissella
Natural Antioxidant Morin
Oxovanadium(Iv) Complex
Radical Scavengers
Tumor Specificity
title_short Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents
title_full Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents
title_fullStr Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents
title_full_unstemmed Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents
title_sort Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents
dc.creator.none.fl_str_mv Naso, Luciana Gissella
Lezama, Luis Raul
Rojo, Teófilo
Etcheverry, Susana Beatriz
Valcarcel, Maria
Roura, Meritxell
Salado, Clarisa
Ferrer, Evelina Gloria
Williams, Patricia Ana María
author Naso, Luciana Gissella
author_facet Naso, Luciana Gissella
Lezama, Luis Raul
Rojo, Teófilo
Etcheverry, Susana Beatriz
Valcarcel, Maria
Roura, Meritxell
Salado, Clarisa
Ferrer, Evelina Gloria
Williams, Patricia Ana María
author_role author
author2 Lezama, Luis Raul
Rojo, Teófilo
Etcheverry, Susana Beatriz
Valcarcel, Maria
Roura, Meritxell
Salado, Clarisa
Ferrer, Evelina Gloria
Williams, Patricia Ana María
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Natural Antioxidant Morin
Oxovanadium(Iv) Complex
Radical Scavengers
Tumor Specificity
topic Natural Antioxidant Morin
Oxovanadium(Iv) Complex
Radical Scavengers
Tumor Specificity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It is known that flavonoids possess, among others, antioxidant and antitumoral properties that depend on their molecular structure. The central objective if this study was to investigate the potential antioxidant and antiproliferative properties of the flavonol morin and its new oxovanadium(IV) complex (VOmor) that was synthesized in order to modify the morin chemical structure. Two osteoblast (UMR106 and MC3T3E1), two breast tumor (T47D and SKBR3) and breast epithelial cell lines in culture were used for the antitumoral determinations. Additionally, a comparative study of their antioxidant capacities using different radicals (DPPH, ABTS+, OH, O2−, ROO) was performed. Selected mechanisms of action were studied using the breast cancer cell lines. Results obtained show that morin and its complex behaved as good antioxidant agents for some of the radicals and that the complexation improved the behavior with respect to OH and O2− radicals being morin more effective as ROO scavenger. A considerable variation in sensitivity was observed in the breast cancer cells but non-specificity was found for the treatment of osteosarcoma. Moreover, the compounds did not affect the normal proliferation of the breast epithelial mammal cells. The mechanistic studies demonstrated that the complex did not generate reactive oxygen species in the cells (confirming the in vitro studies) and did not produce any damage of DNA. The plasmatic membrane was observed to be damaged only in the SKBR3 cell line. In contrast, the perturbation of the mitochondrial membrane potential and the activation of caspase 3/7 for the breast tumor cells revealed an apoptotic cell death process. All these results collectively suggested that VOmor complex could serve as promising pharmacologically active substance against breast cancer treatment.
Fil: Naso, Luciana Gissella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Lezama, Luis Raul. Universidad del País Vasco; España
Fil: Rojo, Teófilo. Universidad del País Vasco; España
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Valcarcel, Maria. Innoprot SL; España
Fil: Roura, Meritxell. Innoprot SL; España
Fil: Salado, Clarisa. Innoprot SL; España
Fil: Ferrer, Evelina Gloria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Williams, Patricia Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
description It is known that flavonoids possess, among others, antioxidant and antitumoral properties that depend on their molecular structure. The central objective if this study was to investigate the potential antioxidant and antiproliferative properties of the flavonol morin and its new oxovanadium(IV) complex (VOmor) that was synthesized in order to modify the morin chemical structure. Two osteoblast (UMR106 and MC3T3E1), two breast tumor (T47D and SKBR3) and breast epithelial cell lines in culture were used for the antitumoral determinations. Additionally, a comparative study of their antioxidant capacities using different radicals (DPPH, ABTS+, OH, O2−, ROO) was performed. Selected mechanisms of action were studied using the breast cancer cell lines. Results obtained show that morin and its complex behaved as good antioxidant agents for some of the radicals and that the complexation improved the behavior with respect to OH and O2− radicals being morin more effective as ROO scavenger. A considerable variation in sensitivity was observed in the breast cancer cells but non-specificity was found for the treatment of osteosarcoma. Moreover, the compounds did not affect the normal proliferation of the breast epithelial mammal cells. The mechanistic studies demonstrated that the complex did not generate reactive oxygen species in the cells (confirming the in vitro studies) and did not produce any damage of DNA. The plasmatic membrane was observed to be damaged only in the SKBR3 cell line. In contrast, the perturbation of the mitochondrial membrane potential and the activation of caspase 3/7 for the breast tumor cells revealed an apoptotic cell death process. All these results collectively suggested that VOmor complex could serve as promising pharmacologically active substance against breast cancer treatment.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24496
Naso, Luciana Gissella; Lezama, Luis Raul; Rojo, Teófilo; Etcheverry, Susana Beatriz; Valcarcel, Maria; et al.; Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents; Elsevier Science; Chemico-biological Interactions; 206; 2; 10-2013; 289-301
0009-2797
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24496
identifier_str_mv Naso, Luciana Gissella; Lezama, Luis Raul; Rojo, Teófilo; Etcheverry, Susana Beatriz; Valcarcel, Maria; et al.; Biological evaluation of morin and its new oxovanadium(IV) complex as antio-xidant and specific anti-cancer agents; Elsevier Science; Chemico-biological Interactions; 206; 2; 10-2013; 289-301
0009-2797
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbi.2013.10.006
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0009279713002603
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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