GD1a modulates GM-CSF-induced cell proliferation

Autores
Santos, A. X. S.; Maia, J. E.; Crespo, Pilar María; Pettenuzzo, L. F.; Daniotti, Jose Luis; Barbé-Tuana, F. M.; Martins, L. M.; Trindade, V. M. T.; Borojevic, R.; Guma, F. C. R.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gangliosides have been extensively described to be involved in the proliferation and differentiation of various cell types, such including hematopoietic cells. Our previous studies on murine models of stroma-mediated myelopoiesis have shown that gangliosides are required for optimal capacity of stromal cells to support proliferation of myeloid precursor cells, being shed to the supernatant and selectively incorporated into myeloid cell membranes. Here we describe the effect of gangliosides on the specific granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation. For that, we used the monocytic FDC-P1 cell line, which is dependent upon GM-CSF for survival and proliferation. Cells were cultured in the presence of GM-CSF and exogenous gangliosides (GM3, GD1a or GM1) or in the absence of endogenous ganglioside synthesis by the use of a ceramide-synthase inhibitor, d-PDMP. We observed that exogenous addition of GD1a enhanced the GM-CSF-induced proliferation of the FDC-P1 cells. Also, we detected an increase in the expression of the α isoform of the GM-CSF receptor (GMRα) as well as of the transcription factor C/EBPα. On the contrary, inhibition of glucosylceramide synthesis was accompanied by a decrease in cell proliferation, which was restored upon the addition of exogenous GD1a. We also show a co-localization of GD1a and GMR by immunocytochemistry. Taken together, our results suggest for the first time that ganglioside GD1a play a role on the modulation of GM-CSF-mediated proliferative response, which might be of great interest not only in hematopoiesis, but also in other immunological processes, Alzheimer disease, alveolar proteinosis and wherever GM-CSF exerts its effects.
Fil: Santos, A. X. S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Maia, J. E.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Crespo, Pilar María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Pettenuzzo, L. F.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Daniotti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Barbé-Tuana, F. M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Martins, L. M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Trindade, V. M. T.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Borojevic, R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Guma, F. C. R.. Universidade Federal do Rio Grande do Sul; Brasil
Materia
GANGLIOSIDES
GM-CSF
CELL PROLIFERATION
HEMATOPOIESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/132298
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/132298
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GD1a modulates GM-CSF-induced cell proliferationSantos, A. X. S.Maia, J. E.Crespo, Pilar MaríaPettenuzzo, L. F.Daniotti, Jose LuisBarbé-Tuana, F. M.Martins, L. M.Trindade, V. M. T.Borojevic, R.Guma, F. C. R.GANGLIOSIDESGM-CSFCELL PROLIFERATIONHEMATOPOIESIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Gangliosides have been extensively described to be involved in the proliferation and differentiation of various cell types, such including hematopoietic cells. Our previous studies on murine models of stroma-mediated myelopoiesis have shown that gangliosides are required for optimal capacity of stromal cells to support proliferation of myeloid precursor cells, being shed to the supernatant and selectively incorporated into myeloid cell membranes. Here we describe the effect of gangliosides on the specific granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation. For that, we used the monocytic FDC-P1 cell line, which is dependent upon GM-CSF for survival and proliferation. Cells were cultured in the presence of GM-CSF and exogenous gangliosides (GM3, GD1a or GM1) or in the absence of endogenous ganglioside synthesis by the use of a ceramide-synthase inhibitor, d-PDMP. We observed that exogenous addition of GD1a enhanced the GM-CSF-induced proliferation of the FDC-P1 cells. Also, we detected an increase in the expression of the α isoform of the GM-CSF receptor (GMRα) as well as of the transcription factor C/EBPα. On the contrary, inhibition of glucosylceramide synthesis was accompanied by a decrease in cell proliferation, which was restored upon the addition of exogenous GD1a. We also show a co-localization of GD1a and GMR by immunocytochemistry. Taken together, our results suggest for the first time that ganglioside GD1a play a role on the modulation of GM-CSF-mediated proliferative response, which might be of great interest not only in hematopoiesis, but also in other immunological processes, Alzheimer disease, alveolar proteinosis and wherever GM-CSF exerts its effects.Fil: Santos, A. X. S.. Universidade Federal do Rio Grande do Sul; BrasilFil: Maia, J. E.. Universidade Federal do Rio Grande do Sul; BrasilFil: Crespo, Pilar María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Pettenuzzo, L. F.. Universidade Federal do Rio Grande do Sul; BrasilFil: Daniotti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Barbé-Tuana, F. M.. Universidade Federal do Rio Grande do Sul; BrasilFil: Martins, L. M.. Universidade Federal do Rio Grande do Sul; BrasilFil: Trindade, V. M. T.. Universidade Federal do Rio Grande do Sul; BrasilFil: Borojevic, R.. Universidade Federal do Rio Grande do Sul; BrasilFil: Guma, F. C. R.. Universidade Federal do Rio Grande do Sul; BrasilAcademic Press Ltd - Elsevier Science Ltd2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132298Santos, A. X. S.; Maia, J. E.; Crespo, Pilar María; Pettenuzzo, L. F.; Daniotti, Jose Luis; et al.; GD1a modulates GM-CSF-induced cell proliferation; Academic Press Ltd - Elsevier Science Ltd; Cytokine; 56; 3; 12-2011; 600-6071043-4666CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.weizmann.ac.il/cytokine/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cyto.2011.08.032info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:03Zoai:ri.conicet.gov.ar:11336/132298instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:04.227CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GD1a modulates GM-CSF-induced cell proliferation
title GD1a modulates GM-CSF-induced cell proliferation
spellingShingle GD1a modulates GM-CSF-induced cell proliferation
Santos, A. X. S.
GANGLIOSIDES
GM-CSF
CELL PROLIFERATION
HEMATOPOIESIS
title_short GD1a modulates GM-CSF-induced cell proliferation
title_full GD1a modulates GM-CSF-induced cell proliferation
title_fullStr GD1a modulates GM-CSF-induced cell proliferation
title_full_unstemmed GD1a modulates GM-CSF-induced cell proliferation
title_sort GD1a modulates GM-CSF-induced cell proliferation
dc.creator.none.fl_str_mv Santos, A. X. S.
Maia, J. E.
Crespo, Pilar María
Pettenuzzo, L. F.
Daniotti, Jose Luis
Barbé-Tuana, F. M.
Martins, L. M.
Trindade, V. M. T.
Borojevic, R.
Guma, F. C. R.
author Santos, A. X. S.
author_facet Santos, A. X. S.
Maia, J. E.
Crespo, Pilar María
Pettenuzzo, L. F.
Daniotti, Jose Luis
Barbé-Tuana, F. M.
Martins, L. M.
Trindade, V. M. T.
Borojevic, R.
Guma, F. C. R.
author_role author
author2 Maia, J. E.
Crespo, Pilar María
Pettenuzzo, L. F.
Daniotti, Jose Luis
Barbé-Tuana, F. M.
Martins, L. M.
Trindade, V. M. T.
Borojevic, R.
Guma, F. C. R.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GANGLIOSIDES
GM-CSF
CELL PROLIFERATION
HEMATOPOIESIS
topic GANGLIOSIDES
GM-CSF
CELL PROLIFERATION
HEMATOPOIESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Gangliosides have been extensively described to be involved in the proliferation and differentiation of various cell types, such including hematopoietic cells. Our previous studies on murine models of stroma-mediated myelopoiesis have shown that gangliosides are required for optimal capacity of stromal cells to support proliferation of myeloid precursor cells, being shed to the supernatant and selectively incorporated into myeloid cell membranes. Here we describe the effect of gangliosides on the specific granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation. For that, we used the monocytic FDC-P1 cell line, which is dependent upon GM-CSF for survival and proliferation. Cells were cultured in the presence of GM-CSF and exogenous gangliosides (GM3, GD1a or GM1) or in the absence of endogenous ganglioside synthesis by the use of a ceramide-synthase inhibitor, d-PDMP. We observed that exogenous addition of GD1a enhanced the GM-CSF-induced proliferation of the FDC-P1 cells. Also, we detected an increase in the expression of the α isoform of the GM-CSF receptor (GMRα) as well as of the transcription factor C/EBPα. On the contrary, inhibition of glucosylceramide synthesis was accompanied by a decrease in cell proliferation, which was restored upon the addition of exogenous GD1a. We also show a co-localization of GD1a and GMR by immunocytochemistry. Taken together, our results suggest for the first time that ganglioside GD1a play a role on the modulation of GM-CSF-mediated proliferative response, which might be of great interest not only in hematopoiesis, but also in other immunological processes, Alzheimer disease, alveolar proteinosis and wherever GM-CSF exerts its effects.
Fil: Santos, A. X. S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Maia, J. E.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Crespo, Pilar María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Pettenuzzo, L. F.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Daniotti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Barbé-Tuana, F. M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Martins, L. M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Trindade, V. M. T.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Borojevic, R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Guma, F. C. R.. Universidade Federal do Rio Grande do Sul; Brasil
description Gangliosides have been extensively described to be involved in the proliferation and differentiation of various cell types, such including hematopoietic cells. Our previous studies on murine models of stroma-mediated myelopoiesis have shown that gangliosides are required for optimal capacity of stromal cells to support proliferation of myeloid precursor cells, being shed to the supernatant and selectively incorporated into myeloid cell membranes. Here we describe the effect of gangliosides on the specific granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation. For that, we used the monocytic FDC-P1 cell line, which is dependent upon GM-CSF for survival and proliferation. Cells were cultured in the presence of GM-CSF and exogenous gangliosides (GM3, GD1a or GM1) or in the absence of endogenous ganglioside synthesis by the use of a ceramide-synthase inhibitor, d-PDMP. We observed that exogenous addition of GD1a enhanced the GM-CSF-induced proliferation of the FDC-P1 cells. Also, we detected an increase in the expression of the α isoform of the GM-CSF receptor (GMRα) as well as of the transcription factor C/EBPα. On the contrary, inhibition of glucosylceramide synthesis was accompanied by a decrease in cell proliferation, which was restored upon the addition of exogenous GD1a. We also show a co-localization of GD1a and GMR by immunocytochemistry. Taken together, our results suggest for the first time that ganglioside GD1a play a role on the modulation of GM-CSF-mediated proliferative response, which might be of great interest not only in hematopoiesis, but also in other immunological processes, Alzheimer disease, alveolar proteinosis and wherever GM-CSF exerts its effects.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/132298
Santos, A. X. S.; Maia, J. E.; Crespo, Pilar María; Pettenuzzo, L. F.; Daniotti, Jose Luis; et al.; GD1a modulates GM-CSF-induced cell proliferation; Academic Press Ltd - Elsevier Science Ltd; Cytokine; 56; 3; 12-2011; 600-607
1043-4666
CONICET Digital
CONICET
url http://hdl.handle.net/11336/132298
identifier_str_mv Santos, A. X. S.; Maia, J. E.; Crespo, Pilar María; Pettenuzzo, L. F.; Daniotti, Jose Luis; et al.; GD1a modulates GM-CSF-induced cell proliferation; Academic Press Ltd - Elsevier Science Ltd; Cytokine; 56; 3; 12-2011; 600-607
1043-4666
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.weizmann.ac.il/cytokine/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cyto.2011.08.032
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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