GD1a modulates GM-CSF-induced cell proliferation
- Autores
- Santos, A. X. S.; Maia, J. E.; Crespo, Pilar María; Pettenuzzo, L. F.; Daniotti, Jose Luis; Barbé-Tuana, F. M.; Martins, L. M.; Trindade, V. M. T.; Borojevic, R.; Guma, F. C. R.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Gangliosides have been extensively described to be involved in the proliferation and differentiation of various cell types, such including hematopoietic cells. Our previous studies on murine models of stroma-mediated myelopoiesis have shown that gangliosides are required for optimal capacity of stromal cells to support proliferation of myeloid precursor cells, being shed to the supernatant and selectively incorporated into myeloid cell membranes. Here we describe the effect of gangliosides on the specific granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation. For that, we used the monocytic FDC-P1 cell line, which is dependent upon GM-CSF for survival and proliferation. Cells were cultured in the presence of GM-CSF and exogenous gangliosides (GM3, GD1a or GM1) or in the absence of endogenous ganglioside synthesis by the use of a ceramide-synthase inhibitor, d-PDMP. We observed that exogenous addition of GD1a enhanced the GM-CSF-induced proliferation of the FDC-P1 cells. Also, we detected an increase in the expression of the α isoform of the GM-CSF receptor (GMRα) as well as of the transcription factor C/EBPα. On the contrary, inhibition of glucosylceramide synthesis was accompanied by a decrease in cell proliferation, which was restored upon the addition of exogenous GD1a. We also show a co-localization of GD1a and GMR by immunocytochemistry. Taken together, our results suggest for the first time that ganglioside GD1a play a role on the modulation of GM-CSF-mediated proliferative response, which might be of great interest not only in hematopoiesis, but also in other immunological processes, Alzheimer disease, alveolar proteinosis and wherever GM-CSF exerts its effects.
Fil: Santos, A. X. S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Maia, J. E.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Crespo, Pilar María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Pettenuzzo, L. F.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Daniotti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Barbé-Tuana, F. M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Martins, L. M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Trindade, V. M. T.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Borojevic, R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Guma, F. C. R.. Universidade Federal do Rio Grande do Sul; Brasil - Materia
-
GANGLIOSIDES
GM-CSF
CELL PROLIFERATION
HEMATOPOIESIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/132298
Ver los metadatos del registro completo
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GD1a modulates GM-CSF-induced cell proliferationSantos, A. X. S.Maia, J. E.Crespo, Pilar MaríaPettenuzzo, L. F.Daniotti, Jose LuisBarbé-Tuana, F. M.Martins, L. M.Trindade, V. M. T.Borojevic, R.Guma, F. C. R.GANGLIOSIDESGM-CSFCELL PROLIFERATIONHEMATOPOIESIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Gangliosides have been extensively described to be involved in the proliferation and differentiation of various cell types, such including hematopoietic cells. Our previous studies on murine models of stroma-mediated myelopoiesis have shown that gangliosides are required for optimal capacity of stromal cells to support proliferation of myeloid precursor cells, being shed to the supernatant and selectively incorporated into myeloid cell membranes. Here we describe the effect of gangliosides on the specific granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation. For that, we used the monocytic FDC-P1 cell line, which is dependent upon GM-CSF for survival and proliferation. Cells were cultured in the presence of GM-CSF and exogenous gangliosides (GM3, GD1a or GM1) or in the absence of endogenous ganglioside synthesis by the use of a ceramide-synthase inhibitor, d-PDMP. We observed that exogenous addition of GD1a enhanced the GM-CSF-induced proliferation of the FDC-P1 cells. Also, we detected an increase in the expression of the α isoform of the GM-CSF receptor (GMRα) as well as of the transcription factor C/EBPα. On the contrary, inhibition of glucosylceramide synthesis was accompanied by a decrease in cell proliferation, which was restored upon the addition of exogenous GD1a. We also show a co-localization of GD1a and GMR by immunocytochemistry. Taken together, our results suggest for the first time that ganglioside GD1a play a role on the modulation of GM-CSF-mediated proliferative response, which might be of great interest not only in hematopoiesis, but also in other immunological processes, Alzheimer disease, alveolar proteinosis and wherever GM-CSF exerts its effects.Fil: Santos, A. X. S.. Universidade Federal do Rio Grande do Sul; BrasilFil: Maia, J. E.. Universidade Federal do Rio Grande do Sul; BrasilFil: Crespo, Pilar María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Pettenuzzo, L. F.. Universidade Federal do Rio Grande do Sul; BrasilFil: Daniotti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Barbé-Tuana, F. M.. Universidade Federal do Rio Grande do Sul; BrasilFil: Martins, L. M.. Universidade Federal do Rio Grande do Sul; BrasilFil: Trindade, V. M. T.. Universidade Federal do Rio Grande do Sul; BrasilFil: Borojevic, R.. Universidade Federal do Rio Grande do Sul; BrasilFil: Guma, F. C. R.. Universidade Federal do Rio Grande do Sul; BrasilAcademic Press Ltd - Elsevier Science Ltd2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132298Santos, A. X. S.; Maia, J. E.; Crespo, Pilar María; Pettenuzzo, L. F.; Daniotti, Jose Luis; et al.; GD1a modulates GM-CSF-induced cell proliferation; Academic Press Ltd - Elsevier Science Ltd; Cytokine; 56; 3; 12-2011; 600-6071043-4666CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.weizmann.ac.il/cytokine/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cyto.2011.08.032info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:15Zoai:ri.conicet.gov.ar:11336/132298instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:15.366CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
GD1a modulates GM-CSF-induced cell proliferation |
title |
GD1a modulates GM-CSF-induced cell proliferation |
spellingShingle |
GD1a modulates GM-CSF-induced cell proliferation Santos, A. X. S. GANGLIOSIDES GM-CSF CELL PROLIFERATION HEMATOPOIESIS |
title_short |
GD1a modulates GM-CSF-induced cell proliferation |
title_full |
GD1a modulates GM-CSF-induced cell proliferation |
title_fullStr |
GD1a modulates GM-CSF-induced cell proliferation |
title_full_unstemmed |
GD1a modulates GM-CSF-induced cell proliferation |
title_sort |
GD1a modulates GM-CSF-induced cell proliferation |
dc.creator.none.fl_str_mv |
Santos, A. X. S. Maia, J. E. Crespo, Pilar María Pettenuzzo, L. F. Daniotti, Jose Luis Barbé-Tuana, F. M. Martins, L. M. Trindade, V. M. T. Borojevic, R. Guma, F. C. R. |
author |
Santos, A. X. S. |
author_facet |
Santos, A. X. S. Maia, J. E. Crespo, Pilar María Pettenuzzo, L. F. Daniotti, Jose Luis Barbé-Tuana, F. M. Martins, L. M. Trindade, V. M. T. Borojevic, R. Guma, F. C. R. |
author_role |
author |
author2 |
Maia, J. E. Crespo, Pilar María Pettenuzzo, L. F. Daniotti, Jose Luis Barbé-Tuana, F. M. Martins, L. M. Trindade, V. M. T. Borojevic, R. Guma, F. C. R. |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
GANGLIOSIDES GM-CSF CELL PROLIFERATION HEMATOPOIESIS |
topic |
GANGLIOSIDES GM-CSF CELL PROLIFERATION HEMATOPOIESIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Gangliosides have been extensively described to be involved in the proliferation and differentiation of various cell types, such including hematopoietic cells. Our previous studies on murine models of stroma-mediated myelopoiesis have shown that gangliosides are required for optimal capacity of stromal cells to support proliferation of myeloid precursor cells, being shed to the supernatant and selectively incorporated into myeloid cell membranes. Here we describe the effect of gangliosides on the specific granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation. For that, we used the monocytic FDC-P1 cell line, which is dependent upon GM-CSF for survival and proliferation. Cells were cultured in the presence of GM-CSF and exogenous gangliosides (GM3, GD1a or GM1) or in the absence of endogenous ganglioside synthesis by the use of a ceramide-synthase inhibitor, d-PDMP. We observed that exogenous addition of GD1a enhanced the GM-CSF-induced proliferation of the FDC-P1 cells. Also, we detected an increase in the expression of the α isoform of the GM-CSF receptor (GMRα) as well as of the transcription factor C/EBPα. On the contrary, inhibition of glucosylceramide synthesis was accompanied by a decrease in cell proliferation, which was restored upon the addition of exogenous GD1a. We also show a co-localization of GD1a and GMR by immunocytochemistry. Taken together, our results suggest for the first time that ganglioside GD1a play a role on the modulation of GM-CSF-mediated proliferative response, which might be of great interest not only in hematopoiesis, but also in other immunological processes, Alzheimer disease, alveolar proteinosis and wherever GM-CSF exerts its effects. Fil: Santos, A. X. S.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Maia, J. E.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Crespo, Pilar María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina Fil: Pettenuzzo, L. F.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Daniotti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina Fil: Barbé-Tuana, F. M.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Martins, L. M.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Trindade, V. M. T.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Borojevic, R.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Guma, F. C. R.. Universidade Federal do Rio Grande do Sul; Brasil |
description |
Gangliosides have been extensively described to be involved in the proliferation and differentiation of various cell types, such including hematopoietic cells. Our previous studies on murine models of stroma-mediated myelopoiesis have shown that gangliosides are required for optimal capacity of stromal cells to support proliferation of myeloid precursor cells, being shed to the supernatant and selectively incorporated into myeloid cell membranes. Here we describe the effect of gangliosides on the specific granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation. For that, we used the monocytic FDC-P1 cell line, which is dependent upon GM-CSF for survival and proliferation. Cells were cultured in the presence of GM-CSF and exogenous gangliosides (GM3, GD1a or GM1) or in the absence of endogenous ganglioside synthesis by the use of a ceramide-synthase inhibitor, d-PDMP. We observed that exogenous addition of GD1a enhanced the GM-CSF-induced proliferation of the FDC-P1 cells. Also, we detected an increase in the expression of the α isoform of the GM-CSF receptor (GMRα) as well as of the transcription factor C/EBPα. On the contrary, inhibition of glucosylceramide synthesis was accompanied by a decrease in cell proliferation, which was restored upon the addition of exogenous GD1a. We also show a co-localization of GD1a and GMR by immunocytochemistry. Taken together, our results suggest for the first time that ganglioside GD1a play a role on the modulation of GM-CSF-mediated proliferative response, which might be of great interest not only in hematopoiesis, but also in other immunological processes, Alzheimer disease, alveolar proteinosis and wherever GM-CSF exerts its effects. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/132298 Santos, A. X. S.; Maia, J. E.; Crespo, Pilar María; Pettenuzzo, L. F.; Daniotti, Jose Luis; et al.; GD1a modulates GM-CSF-induced cell proliferation; Academic Press Ltd - Elsevier Science Ltd; Cytokine; 56; 3; 12-2011; 600-607 1043-4666 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/132298 |
identifier_str_mv |
Santos, A. X. S.; Maia, J. E.; Crespo, Pilar María; Pettenuzzo, L. F.; Daniotti, Jose Luis; et al.; GD1a modulates GM-CSF-induced cell proliferation; Academic Press Ltd - Elsevier Science Ltd; Cytokine; 56; 3; 12-2011; 600-607 1043-4666 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.weizmann.ac.il/cytokine/ info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cyto.2011.08.032 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Ltd - Elsevier Science Ltd |
publisher.none.fl_str_mv |
Academic Press Ltd - Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269274232586240 |
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13.13397 |