Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila

Autores
Urbieta, María Sofía; Rascovan, Nicolas; Castro, Camila Andrea; Giaveno, Alejandra; Vazquez, Martín; Donati, Edgardo Ruben
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Copahue geothermal area is located on the Northwest corner of Neuquén, crowned by Copahue volcano (30000 m o.s.l. in Cordillera de los Andes). The constant volcanic activity has determined the extreme environments found in the area, specially the existence of many high-temperature, acidic, sulphur-reach ponds and hot springs. Such habitats are populated by extremophilic microorganisms well adapted to develop under hard physicochemical conditions and the lack of organic carbon sources. In acidic high temperature environments archaea are important members of the communities, however many of the species are yet unknown and uncharacterized. Acidianus copahuensis is a thermoacidophilic archaea (phylum Crenarchaeota, order Sulfolobales) that has been isolated from various hot springs in Copahue and, until now, not reported anywhere else. A. copahuensis is a facultative chemolithoautotroph that grows optimally at 75°C and pH 2.5. It is capable of autotrophic growth; aerobically using iron and different sulfur compounds as energy sources, and anaerobically using H2 and S as electron donors and Fe(III) or S as final electron acceptors. It can also grow heterotrophically using yeast extract or glucose. In this work we present the first analysis of the genome sequence of A. copahuensis, obtained using a whole-genome shotgun (WGS) strategy with a 454-FLX Titanium pyrosequencer. The draft genome is 2,454,023 bases in length and the G+C content is 35.63 mol%. According to RAST annotation a total of 2,548 coding sequences (CDSs) and 52 structural RNAs (49 tRNAs, 3 rRNA) were predicted. A 47% of the CDSs were classified as hypothetical proteins and 20% as known enzymes. The genome of A. copahuensis reveled specific genes that could be associated with the strains metabolic activities. The key enzymes for sulphur compounds oxidation described for other acidophilic archea, such as sulfur oxigenase-reductase (SOR) and thiosulfate-quinone oxidoreductase (TQO), were detected in A. copahuensis. Iron oxidation as an energy source was also represented in the genome by some of the fox cluster enzymes. A. copahuensis presents genes coding for proteins of the five major terminal oxidase complexes of Sulfolobales, so far reported in only two species. As regards autotrophic metabolism, carbon fixation through the 3-hydroxypropionate?4-hydroxybutyrate cycle could be inferred by the presence of the key enzymes of this pathway. An interesting discovery is the presence of genes encoding for arsenite oxidase (aioAB), not common in Sulfolobales. This could be related with the use of arsenite as an energy source or the arsenic bioremediation potential of A. copahuensis. The present analysis confirms the presence of key enzymes that allow A. copahuensis survival in the environmental conditions of Copahue and make it a good candidate for biomining of sulphide minerals. Further analysis would be required to for a better understanding of these archaea extreme metabolic features.
Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Rascovan, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Castro, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Giaveno, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue | Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue; Argentina
Fil: Vazquez, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
X Congreso Argentino de Microbiología General
Mar del Plata
Argentina
Sociedad Argentina de Microbiología General
Materia
ACIDIANUS COPAHUENSIS
THERMOPHILIC ARCHAEA
DRAFT GENOME
COPAHUE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195232
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófilaA deeper look into extremophiles: analysis of the draft genome of Acidianus copahuensis, a novel thermoacidophilic archaea isolated from Copahue geothermal areaUrbieta, María SofíaRascovan, NicolasCastro, Camila AndreaGiaveno, AlejandraVazquez, MartínDonati, Edgardo RubenACIDIANUS COPAHUENSISTHERMOPHILIC ARCHAEADRAFT GENOMECOPAHUEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Copahue geothermal area is located on the Northwest corner of Neuquén, crowned by Copahue volcano (30000 m o.s.l. in Cordillera de los Andes). The constant volcanic activity has determined the extreme environments found in the area, specially the existence of many high-temperature, acidic, sulphur-reach ponds and hot springs. Such habitats are populated by extremophilic microorganisms well adapted to develop under hard physicochemical conditions and the lack of organic carbon sources. In acidic high temperature environments archaea are important members of the communities, however many of the species are yet unknown and uncharacterized. Acidianus copahuensis is a thermoacidophilic archaea (phylum Crenarchaeota, order Sulfolobales) that has been isolated from various hot springs in Copahue and, until now, not reported anywhere else. A. copahuensis is a facultative chemolithoautotroph that grows optimally at 75°C and pH 2.5. It is capable of autotrophic growth; aerobically using iron and different sulfur compounds as energy sources, and anaerobically using H2 and S as electron donors and Fe(III) or S as final electron acceptors. It can also grow heterotrophically using yeast extract or glucose. In this work we present the first analysis of the genome sequence of A. copahuensis, obtained using a whole-genome shotgun (WGS) strategy with a 454-FLX Titanium pyrosequencer. The draft genome is 2,454,023 bases in length and the G+C content is 35.63 mol%. According to RAST annotation a total of 2,548 coding sequences (CDSs) and 52 structural RNAs (49 tRNAs, 3 rRNA) were predicted. A 47% of the CDSs were classified as hypothetical proteins and 20% as known enzymes. The genome of A. copahuensis reveled specific genes that could be associated with the strains metabolic activities. The key enzymes for sulphur compounds oxidation described for other acidophilic archea, such as sulfur oxigenase-reductase (SOR) and thiosulfate-quinone oxidoreductase (TQO), were detected in A. copahuensis. Iron oxidation as an energy source was also represented in the genome by some of the fox cluster enzymes. A. copahuensis presents genes coding for proteins of the five major terminal oxidase complexes of Sulfolobales, so far reported in only two species. As regards autotrophic metabolism, carbon fixation through the 3-hydroxypropionate?4-hydroxybutyrate cycle could be inferred by the presence of the key enzymes of this pathway. An interesting discovery is the presence of genes encoding for arsenite oxidase (aioAB), not common in Sulfolobales. This could be related with the use of arsenite as an energy source or the arsenic bioremediation potential of A. copahuensis. The present analysis confirms the presence of key enzymes that allow A. copahuensis survival in the environmental conditions of Copahue and make it a good candidate for biomining of sulphide minerals. Further analysis would be required to for a better understanding of these archaea extreme metabolic features.Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Rascovan, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Castro, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Giaveno, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue | Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue; ArgentinaFil: Vazquez, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaX Congreso Argentino de Microbiología GeneralMar del PlataArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195232Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila; X Congreso Argentino de Microbiología General; Mar del Plata; Argentina; 2014; 1-2CONICET DigitalCONICETengNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:05Zoai:ri.conicet.gov.ar:11336/195232instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:05.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila
A deeper look into extremophiles: analysis of the draft genome of Acidianus copahuensis, a novel thermoacidophilic archaea isolated from Copahue geothermal area
title Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila
spellingShingle Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila
Urbieta, María Sofía
ACIDIANUS COPAHUENSIS
THERMOPHILIC ARCHAEA
DRAFT GENOME
COPAHUE
title_short Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila
title_full Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila
title_fullStr Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila
title_full_unstemmed Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila
title_sort Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila
dc.creator.none.fl_str_mv Urbieta, María Sofía
Rascovan, Nicolas
Castro, Camila Andrea
Giaveno, Alejandra
Vazquez, Martín
Donati, Edgardo Ruben
author Urbieta, María Sofía
author_facet Urbieta, María Sofía
Rascovan, Nicolas
Castro, Camila Andrea
Giaveno, Alejandra
Vazquez, Martín
Donati, Edgardo Ruben
author_role author
author2 Rascovan, Nicolas
Castro, Camila Andrea
Giaveno, Alejandra
Vazquez, Martín
Donati, Edgardo Ruben
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ACIDIANUS COPAHUENSIS
THERMOPHILIC ARCHAEA
DRAFT GENOME
COPAHUE
topic ACIDIANUS COPAHUENSIS
THERMOPHILIC ARCHAEA
DRAFT GENOME
COPAHUE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Copahue geothermal area is located on the Northwest corner of Neuquén, crowned by Copahue volcano (30000 m o.s.l. in Cordillera de los Andes). The constant volcanic activity has determined the extreme environments found in the area, specially the existence of many high-temperature, acidic, sulphur-reach ponds and hot springs. Such habitats are populated by extremophilic microorganisms well adapted to develop under hard physicochemical conditions and the lack of organic carbon sources. In acidic high temperature environments archaea are important members of the communities, however many of the species are yet unknown and uncharacterized. Acidianus copahuensis is a thermoacidophilic archaea (phylum Crenarchaeota, order Sulfolobales) that has been isolated from various hot springs in Copahue and, until now, not reported anywhere else. A. copahuensis is a facultative chemolithoautotroph that grows optimally at 75°C and pH 2.5. It is capable of autotrophic growth; aerobically using iron and different sulfur compounds as energy sources, and anaerobically using H2 and S as electron donors and Fe(III) or S as final electron acceptors. It can also grow heterotrophically using yeast extract or glucose. In this work we present the first analysis of the genome sequence of A. copahuensis, obtained using a whole-genome shotgun (WGS) strategy with a 454-FLX Titanium pyrosequencer. The draft genome is 2,454,023 bases in length and the G+C content is 35.63 mol%. According to RAST annotation a total of 2,548 coding sequences (CDSs) and 52 structural RNAs (49 tRNAs, 3 rRNA) were predicted. A 47% of the CDSs were classified as hypothetical proteins and 20% as known enzymes. The genome of A. copahuensis reveled specific genes that could be associated with the strains metabolic activities. The key enzymes for sulphur compounds oxidation described for other acidophilic archea, such as sulfur oxigenase-reductase (SOR) and thiosulfate-quinone oxidoreductase (TQO), were detected in A. copahuensis. Iron oxidation as an energy source was also represented in the genome by some of the fox cluster enzymes. A. copahuensis presents genes coding for proteins of the five major terminal oxidase complexes of Sulfolobales, so far reported in only two species. As regards autotrophic metabolism, carbon fixation through the 3-hydroxypropionate?4-hydroxybutyrate cycle could be inferred by the presence of the key enzymes of this pathway. An interesting discovery is the presence of genes encoding for arsenite oxidase (aioAB), not common in Sulfolobales. This could be related with the use of arsenite as an energy source or the arsenic bioremediation potential of A. copahuensis. The present analysis confirms the presence of key enzymes that allow A. copahuensis survival in the environmental conditions of Copahue and make it a good candidate for biomining of sulphide minerals. Further analysis would be required to for a better understanding of these archaea extreme metabolic features.
Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Rascovan, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Castro, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Giaveno, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue | Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue; Argentina
Fil: Vazquez, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
X Congreso Argentino de Microbiología General
Mar del Plata
Argentina
Sociedad Argentina de Microbiología General
description Copahue geothermal area is located on the Northwest corner of Neuquén, crowned by Copahue volcano (30000 m o.s.l. in Cordillera de los Andes). The constant volcanic activity has determined the extreme environments found in the area, specially the existence of many high-temperature, acidic, sulphur-reach ponds and hot springs. Such habitats are populated by extremophilic microorganisms well adapted to develop under hard physicochemical conditions and the lack of organic carbon sources. In acidic high temperature environments archaea are important members of the communities, however many of the species are yet unknown and uncharacterized. Acidianus copahuensis is a thermoacidophilic archaea (phylum Crenarchaeota, order Sulfolobales) that has been isolated from various hot springs in Copahue and, until now, not reported anywhere else. A. copahuensis is a facultative chemolithoautotroph that grows optimally at 75°C and pH 2.5. It is capable of autotrophic growth; aerobically using iron and different sulfur compounds as energy sources, and anaerobically using H2 and S as electron donors and Fe(III) or S as final electron acceptors. It can also grow heterotrophically using yeast extract or glucose. In this work we present the first analysis of the genome sequence of A. copahuensis, obtained using a whole-genome shotgun (WGS) strategy with a 454-FLX Titanium pyrosequencer. The draft genome is 2,454,023 bases in length and the G+C content is 35.63 mol%. According to RAST annotation a total of 2,548 coding sequences (CDSs) and 52 structural RNAs (49 tRNAs, 3 rRNA) were predicted. A 47% of the CDSs were classified as hypothetical proteins and 20% as known enzymes. The genome of A. copahuensis reveled specific genes that could be associated with the strains metabolic activities. The key enzymes for sulphur compounds oxidation described for other acidophilic archea, such as sulfur oxigenase-reductase (SOR) and thiosulfate-quinone oxidoreductase (TQO), were detected in A. copahuensis. Iron oxidation as an energy source was also represented in the genome by some of the fox cluster enzymes. A. copahuensis presents genes coding for proteins of the five major terminal oxidase complexes of Sulfolobales, so far reported in only two species. As regards autotrophic metabolism, carbon fixation through the 3-hydroxypropionate?4-hydroxybutyrate cycle could be inferred by the presence of the key enzymes of this pathway. An interesting discovery is the presence of genes encoding for arsenite oxidase (aioAB), not common in Sulfolobales. This could be related with the use of arsenite as an energy source or the arsenic bioremediation potential of A. copahuensis. The present analysis confirms the presence of key enzymes that allow A. copahuensis survival in the environmental conditions of Copahue and make it a good candidate for biomining of sulphide minerals. Further analysis would be required to for a better understanding of these archaea extreme metabolic features.
publishDate 2014
dc.date.none.fl_str_mv 2014
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info:eu-repo/semantics/conferenceObject
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195232
Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila; X Congreso Argentino de Microbiología General; Mar del Plata; Argentina; 2014; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195232
identifier_str_mv Una mirada más profunda dentro de los extremofilos: análisis del genoma de una nueva especie de arquea termoacidófila; X Congreso Argentino de Microbiología General; Mar del Plata; Argentina; 2014; 1-2
CONICET Digital
CONICET
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dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
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