Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus

Autores
Castro, Camila Andrea; Vera, Mario; Donati, Edgardo Ruben; Sand, Wolfgang
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we have studied the attachment and colonization of pyrite surfaces by the recently isolated thermophilic archaeon Candidatus Acidianus copahuensis (from the geothermal Caviahue-Copahue system, Argentina). Cells pregrown with sulfur, iron(II) or pyrite were tested. In order to characterize the EPS glycoconjugates of this strain, fluorescent lectins were used. Concanavalin A (ConA) gave the best signal and was selected for further studies. Coupons and grains of pyrite were treated with DAPI (to stain attached cells) and TRITC-ConA (to stain polysaccharides from EPS). Pyrite surfaces were imaged by epifluorescence (EFM) and confocal laser microscopy (CLSM). Initial cell attachment to pyrite grains was estimated to be 26%, 35% and 43% when cells were pregrown with sulfur, iron(II) and pyrite as electron donors, respectively. It was observed that the cell adhesion correlated with an increase of EPS production; both processes were favoured when cells were pregrown with pyrite or iron(II). Also the effect of inorganic phosphate (Pi) starvation in the attachment of Acidianus was studied in similar tests using a base medium without Pi. An increase of cell attachment under Pi starvation conditions was detectable.
Fil: Castro, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Vera, Mario. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Sand, Wolfgang. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
Materia
Acidianus
Archaea
Biofilm
Bioleaching
Eps
Thermophiles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4306
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4306
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Visualization of attachment and colonization of pyrite surfaces by a novel species of AcidianusCastro, Camila AndreaVera, MarioDonati, Edgardo RubenSand, WolfgangAcidianusArchaeaBiofilmBioleachingEpsThermophileshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2In this work we have studied the attachment and colonization of pyrite surfaces by the recently isolated thermophilic archaeon Candidatus Acidianus copahuensis (from the geothermal Caviahue-Copahue system, Argentina). Cells pregrown with sulfur, iron(II) or pyrite were tested. In order to characterize the EPS glycoconjugates of this strain, fluorescent lectins were used. Concanavalin A (ConA) gave the best signal and was selected for further studies. Coupons and grains of pyrite were treated with DAPI (to stain attached cells) and TRITC-ConA (to stain polysaccharides from EPS). Pyrite surfaces were imaged by epifluorescence (EFM) and confocal laser microscopy (CLSM). Initial cell attachment to pyrite grains was estimated to be 26%, 35% and 43% when cells were pregrown with sulfur, iron(II) and pyrite as electron donors, respectively. It was observed that the cell adhesion correlated with an increase of EPS production; both processes were favoured when cells were pregrown with pyrite or iron(II). Also the effect of inorganic phosphate (Pi) starvation in the attachment of Acidianus was studied in similar tests using a base medium without Pi. An increase of cell attachment under Pi starvation conditions was detectable.Fil: Castro, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Vera, Mario. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; AlemaniaFil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Sand, Wolfgang. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; AlemaniaTrans Tech Publications2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4306Castro, Camila Andrea; Vera, Mario; Donati, Edgardo Ruben; Sand, Wolfgang; Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus; Trans Tech Publications; Advanced Materials Research; 825; 10-2013; 70-731022-6680enginfo:eu-repo/semantics/altIdentifier/url/http://www.scientific.net/AMR.825.70info:eu-repo/semantics/altIdentifier/doi/10.4028/www.scientific.net/AMR.825.70info:eu-repo/semantics/altIdentifier/issn/1022-6680info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:16Zoai:ri.conicet.gov.ar:11336/4306instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:17.035CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus
title Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus
spellingShingle Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus
Castro, Camila Andrea
Acidianus
Archaea
Biofilm
Bioleaching
Eps
Thermophiles
title_short Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus
title_full Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus
title_fullStr Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus
title_full_unstemmed Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus
title_sort Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus
dc.creator.none.fl_str_mv Castro, Camila Andrea
Vera, Mario
Donati, Edgardo Ruben
Sand, Wolfgang
author Castro, Camila Andrea
author_facet Castro, Camila Andrea
Vera, Mario
Donati, Edgardo Ruben
Sand, Wolfgang
author_role author
author2 Vera, Mario
Donati, Edgardo Ruben
Sand, Wolfgang
author2_role author
author
author
dc.subject.none.fl_str_mv Acidianus
Archaea
Biofilm
Bioleaching
Eps
Thermophiles
topic Acidianus
Archaea
Biofilm
Bioleaching
Eps
Thermophiles
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In this work we have studied the attachment and colonization of pyrite surfaces by the recently isolated thermophilic archaeon Candidatus Acidianus copahuensis (from the geothermal Caviahue-Copahue system, Argentina). Cells pregrown with sulfur, iron(II) or pyrite were tested. In order to characterize the EPS glycoconjugates of this strain, fluorescent lectins were used. Concanavalin A (ConA) gave the best signal and was selected for further studies. Coupons and grains of pyrite were treated with DAPI (to stain attached cells) and TRITC-ConA (to stain polysaccharides from EPS). Pyrite surfaces were imaged by epifluorescence (EFM) and confocal laser microscopy (CLSM). Initial cell attachment to pyrite grains was estimated to be 26%, 35% and 43% when cells were pregrown with sulfur, iron(II) and pyrite as electron donors, respectively. It was observed that the cell adhesion correlated with an increase of EPS production; both processes were favoured when cells were pregrown with pyrite or iron(II). Also the effect of inorganic phosphate (Pi) starvation in the attachment of Acidianus was studied in similar tests using a base medium without Pi. An increase of cell attachment under Pi starvation conditions was detectable.
Fil: Castro, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Vera, Mario. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Sand, Wolfgang. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
description In this work we have studied the attachment and colonization of pyrite surfaces by the recently isolated thermophilic archaeon Candidatus Acidianus copahuensis (from the geothermal Caviahue-Copahue system, Argentina). Cells pregrown with sulfur, iron(II) or pyrite were tested. In order to characterize the EPS glycoconjugates of this strain, fluorescent lectins were used. Concanavalin A (ConA) gave the best signal and was selected for further studies. Coupons and grains of pyrite were treated with DAPI (to stain attached cells) and TRITC-ConA (to stain polysaccharides from EPS). Pyrite surfaces were imaged by epifluorescence (EFM) and confocal laser microscopy (CLSM). Initial cell attachment to pyrite grains was estimated to be 26%, 35% and 43% when cells were pregrown with sulfur, iron(II) and pyrite as electron donors, respectively. It was observed that the cell adhesion correlated with an increase of EPS production; both processes were favoured when cells were pregrown with pyrite or iron(II). Also the effect of inorganic phosphate (Pi) starvation in the attachment of Acidianus was studied in similar tests using a base medium without Pi. An increase of cell attachment under Pi starvation conditions was detectable.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4306
Castro, Camila Andrea; Vera, Mario; Donati, Edgardo Ruben; Sand, Wolfgang; Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus; Trans Tech Publications; Advanced Materials Research; 825; 10-2013; 70-73
1022-6680
url http://hdl.handle.net/11336/4306
identifier_str_mv Castro, Camila Andrea; Vera, Mario; Donati, Edgardo Ruben; Sand, Wolfgang; Visualization of attachment and colonization of pyrite surfaces by a novel species of Acidianus; Trans Tech Publications; Advanced Materials Research; 825; 10-2013; 70-73
1022-6680
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scientific.net/AMR.825.70
info:eu-repo/semantics/altIdentifier/doi/10.4028/www.scientific.net/AMR.825.70
info:eu-repo/semantics/altIdentifier/issn/1022-6680
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Trans Tech Publications
publisher.none.fl_str_mv Trans Tech Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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