Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fe...

Autores
Veaute, Carolina Melania Isabel; Furlong, Laura Ines; Cameo, Mónica; Harris, Jeffrey D.; Vazquez, Monica Hebe
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To assess the effect of antiacrosin antibodies upon proacrosin/acrosin activities and animal fertility. Design: Prospective study. Setting: Basic research laboratory. Patient(s): A gene immunization (GI) model was developed; mice were injected with the sequence encoding human proacrosin (h-proacrosin), cloned in an expression vector. Intervention(s): Subcloning of h-proacrosin in a eukaryotic expression vector (promoter, CMV; leader sequence, a-1 antitrypsin; pSF2-Acro); GI of female mice with this plasmid. Main Outcome Measure(s): The following parameters were evaluated: [1] adequate conditions for GI protocols, [2] humoral response to GI with pSF2-Acro, [3] protein regions recognized by the antibodies, and [4] effect of antibodies upon proacrosin/acrosin–ZPA binding and amidase activity, and animal fertility. Result(s): Conditions of female mice GI with the proacrosin sequence were established (plasmid purification with anion exchange chromatography and 40 mg of pSF2-Acro per dose) to trigger an immune response, reaching maximum levels at week 9 after the first injection. Antibodies produced by GI recognized human and mouse sperm acrosin systems, inhibited human proacrosin/acrosin interaction with recombinant human ZPA and protease activity, and negatively affected mouse IVF and early embryonic development. In addition, mice immunized with SF2-Acro exhibited a significantly lower size of fetuses. Conclusion(s): Antiacrosin antibodies developed by using GI inhibit human proacrosin/acrosin activities and impair mouse fertility.
Fil: Veaute, Carolina Melania Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Furlong, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cameo, Mónica. Laboratorio Biología de la Reproducción; Argentina
Fil: Harris, Jeffrey D.. Zonagen; Estados Unidos
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
Proacrosin
Spermatozoa
Gene Immunization
Antisperm Antibodies
Acrosin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24462
Acceder
id CONICETDig_83b5ce7e79af9bbb8e35505292bdd98d
oai_identifier_str oai:ri.conicet.gov.ar:11336/24462
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertilityVeaute, Carolina Melania IsabelFurlong, Laura InesCameo, MónicaHarris, Jeffrey D.Vazquez, Monica HebeProacrosinSpermatozoaGene ImmunizationAntisperm AntibodiesAcrosinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: To assess the effect of antiacrosin antibodies upon proacrosin/acrosin activities and animal fertility. Design: Prospective study. Setting: Basic research laboratory. Patient(s): A gene immunization (GI) model was developed; mice were injected with the sequence encoding human proacrosin (h-proacrosin), cloned in an expression vector. Intervention(s): Subcloning of h-proacrosin in a eukaryotic expression vector (promoter, CMV; leader sequence, a-1 antitrypsin; pSF2-Acro); GI of female mice with this plasmid. Main Outcome Measure(s): The following parameters were evaluated: [1] adequate conditions for GI protocols, [2] humoral response to GI with pSF2-Acro, [3] protein regions recognized by the antibodies, and [4] effect of antibodies upon proacrosin/acrosin–ZPA binding and amidase activity, and animal fertility. Result(s): Conditions of female mice GI with the proacrosin sequence were established (plasmid purification with anion exchange chromatography and 40 mg of pSF2-Acro per dose) to trigger an immune response, reaching maximum levels at week 9 after the first injection. Antibodies produced by GI recognized human and mouse sperm acrosin systems, inhibited human proacrosin/acrosin interaction with recombinant human ZPA and protease activity, and negatively affected mouse IVF and early embryonic development. In addition, mice immunized with SF2-Acro exhibited a significantly lower size of fetuses. Conclusion(s): Antiacrosin antibodies developed by using GI inhibit human proacrosin/acrosin activities and impair mouse fertility.Fil: Veaute, Carolina Melania Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Furlong, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Cameo, Mónica. Laboratorio Biología de la Reproducción; ArgentinaFil: Harris, Jeffrey D.. Zonagen; Estados UnidosFil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaElsevier2008-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24462Veaute, Carolina Melania Isabel; Furlong, Laura Ines; Cameo, Mónica; Harris, Jeffrey D.; Vazquez, Monica Hebe; Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility; Elsevier; Fertility and Sterility; 91; 4; 4-2008; 1256-12680015-02821556-5653CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S001502820800174Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.01.082info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:59Zoai:ri.conicet.gov.ar:11336/24462instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:00.312CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility
title Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility
spellingShingle Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility
Veaute, Carolina Melania Isabel
Proacrosin
Spermatozoa
Gene Immunization
Antisperm Antibodies
Acrosin
title_short Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility
title_full Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility
title_fullStr Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility
title_full_unstemmed Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility
title_sort Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility
dc.creator.none.fl_str_mv Veaute, Carolina Melania Isabel
Furlong, Laura Ines
Cameo, Mónica
Harris, Jeffrey D.
Vazquez, Monica Hebe
author Veaute, Carolina Melania Isabel
author_facet Veaute, Carolina Melania Isabel
Furlong, Laura Ines
Cameo, Mónica
Harris, Jeffrey D.
Vazquez, Monica Hebe
author_role author
author2 Furlong, Laura Ines
Cameo, Mónica
Harris, Jeffrey D.
Vazquez, Monica Hebe
author2_role author
author
author
author
dc.subject.none.fl_str_mv Proacrosin
Spermatozoa
Gene Immunization
Antisperm Antibodies
Acrosin
topic Proacrosin
Spermatozoa
Gene Immunization
Antisperm Antibodies
Acrosin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: To assess the effect of antiacrosin antibodies upon proacrosin/acrosin activities and animal fertility. Design: Prospective study. Setting: Basic research laboratory. Patient(s): A gene immunization (GI) model was developed; mice were injected with the sequence encoding human proacrosin (h-proacrosin), cloned in an expression vector. Intervention(s): Subcloning of h-proacrosin in a eukaryotic expression vector (promoter, CMV; leader sequence, a-1 antitrypsin; pSF2-Acro); GI of female mice with this plasmid. Main Outcome Measure(s): The following parameters were evaluated: [1] adequate conditions for GI protocols, [2] humoral response to GI with pSF2-Acro, [3] protein regions recognized by the antibodies, and [4] effect of antibodies upon proacrosin/acrosin–ZPA binding and amidase activity, and animal fertility. Result(s): Conditions of female mice GI with the proacrosin sequence were established (plasmid purification with anion exchange chromatography and 40 mg of pSF2-Acro per dose) to trigger an immune response, reaching maximum levels at week 9 after the first injection. Antibodies produced by GI recognized human and mouse sperm acrosin systems, inhibited human proacrosin/acrosin interaction with recombinant human ZPA and protease activity, and negatively affected mouse IVF and early embryonic development. In addition, mice immunized with SF2-Acro exhibited a significantly lower size of fetuses. Conclusion(s): Antiacrosin antibodies developed by using GI inhibit human proacrosin/acrosin activities and impair mouse fertility.
Fil: Veaute, Carolina Melania Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Furlong, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cameo, Mónica. Laboratorio Biología de la Reproducción; Argentina
Fil: Harris, Jeffrey D.. Zonagen; Estados Unidos
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Objective: To assess the effect of antiacrosin antibodies upon proacrosin/acrosin activities and animal fertility. Design: Prospective study. Setting: Basic research laboratory. Patient(s): A gene immunization (GI) model was developed; mice were injected with the sequence encoding human proacrosin (h-proacrosin), cloned in an expression vector. Intervention(s): Subcloning of h-proacrosin in a eukaryotic expression vector (promoter, CMV; leader sequence, a-1 antitrypsin; pSF2-Acro); GI of female mice with this plasmid. Main Outcome Measure(s): The following parameters were evaluated: [1] adequate conditions for GI protocols, [2] humoral response to GI with pSF2-Acro, [3] protein regions recognized by the antibodies, and [4] effect of antibodies upon proacrosin/acrosin–ZPA binding and amidase activity, and animal fertility. Result(s): Conditions of female mice GI with the proacrosin sequence were established (plasmid purification with anion exchange chromatography and 40 mg of pSF2-Acro per dose) to trigger an immune response, reaching maximum levels at week 9 after the first injection. Antibodies produced by GI recognized human and mouse sperm acrosin systems, inhibited human proacrosin/acrosin interaction with recombinant human ZPA and protease activity, and negatively affected mouse IVF and early embryonic development. In addition, mice immunized with SF2-Acro exhibited a significantly lower size of fetuses. Conclusion(s): Antiacrosin antibodies developed by using GI inhibit human proacrosin/acrosin activities and impair mouse fertility.
publishDate 2008
dc.date.none.fl_str_mv 2008-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24462
Veaute, Carolina Melania Isabel; Furlong, Laura Ines; Cameo, Mónica; Harris, Jeffrey D.; Vazquez, Monica Hebe; Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility; Elsevier; Fertility and Sterility; 91; 4; 4-2008; 1256-1268
0015-0282
1556-5653
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24462
identifier_str_mv Veaute, Carolina Melania Isabel; Furlong, Laura Ines; Cameo, Mónica; Harris, Jeffrey D.; Vazquez, Monica Hebe; Antiacrosin antibodies and infertility. II: gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility; Elsevier; Fertility and Sterility; 91; 4; 4-2008; 1256-1268
0015-0282
1556-5653
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S001502820800174X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.01.082
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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