Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth

Autores
Bommer, Imke; Juriol, Lorena Vanesa; Muzzio, Damián Oscar; Valeff, Natalin Jimena; Ehrhardt, Jens; Matzner, Franziska; Ziegler, Katharina; Malinowsky, Kristin; Ventimiglia, Maria Silvia; Zygmunt, Marek; Jensen, Cristian Federico
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The amniotic fluid provides mechanical protection and immune defense against pathogens to the fetus. Indeed, components of the innate and adaptive immunity, including B cells, have been described in the amniotic fluid. However, limited information concerning phenotype and functionality of amniotic fluid B cells is available. Hence, we aimed to perform a full phenotypical and functional characterization of amniotic fluid B cells in normal pregnancy and in a mouse model of preterm birth. Phenotypic analysis depicted the presence of two populations of amniotic fluid B cells: an immature population, resembling B1 progenitor cells and a more mature population. Further isolation and in vitro co-culture with a bone marrow stroma cell line demonstrated the capacity of the immature B cells to mature. This was further supported by spontaneous production of IgM, a feature of the B1 B cell sub-population. An additional in vitro stimulation with lipopolysaccharide induced the activation of amniotic fluid B cells as well as the production of pro and anti-inflammatory cytokines. Furthermore, amniotic fluid B cells were expanded in the acute phase of LPS-induced preterm birth. Overall our data add new insight not only on the phenotype and developmental stage of the amniotic fluid B1 B cells but especially on their functionality. This provides important information for a better understanding of their role within the amniotic fluid as immunological protective barrier, especially with regard to intraamniotic infection and preterm birth.
Fil: Bommer, Imke. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Juriol, Lorena Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Muzzio, Damián Oscar. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Valeff, Natalin Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Ehrhardt, Jens. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Matzner, Franziska. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Ziegler, Katharina. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Malinowsky, Kristin. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Ventimiglia, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Zygmunt, Marek. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Jensen, Cristian Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad Nacional Arturo Jauretche; Argentina
Materia
B cells
Pregnancy
Preterm birth
Amniotic fluid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/120089
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/120089
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birthBommer, ImkeJuriol, Lorena VanesaMuzzio, Damián OscarValeff, Natalin JimenaEhrhardt, JensMatzner, FranziskaZiegler, KatharinaMalinowsky, KristinVentimiglia, Maria SilviaZygmunt, MarekJensen, Cristian FedericoB cellsPregnancyPreterm birthAmniotic fluidhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The amniotic fluid provides mechanical protection and immune defense against pathogens to the fetus. Indeed, components of the innate and adaptive immunity, including B cells, have been described in the amniotic fluid. However, limited information concerning phenotype and functionality of amniotic fluid B cells is available. Hence, we aimed to perform a full phenotypical and functional characterization of amniotic fluid B cells in normal pregnancy and in a mouse model of preterm birth. Phenotypic analysis depicted the presence of two populations of amniotic fluid B cells: an immature population, resembling B1 progenitor cells and a more mature population. Further isolation and in vitro co-culture with a bone marrow stroma cell line demonstrated the capacity of the immature B cells to mature. This was further supported by spontaneous production of IgM, a feature of the B1 B cell sub-population. An additional in vitro stimulation with lipopolysaccharide induced the activation of amniotic fluid B cells as well as the production of pro and anti-inflammatory cytokines. Furthermore, amniotic fluid B cells were expanded in the acute phase of LPS-induced preterm birth. Overall our data add new insight not only on the phenotype and developmental stage of the amniotic fluid B1 B cells but especially on their functionality. This provides important information for a better understanding of their role within the amniotic fluid as immunological protective barrier, especially with regard to intraamniotic infection and preterm birth.Fil: Bommer, Imke. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Juriol, Lorena Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Muzzio, Damián Oscar. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Valeff, Natalin Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Ehrhardt, Jens. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Matzner, Franziska. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Ziegler, Katharina. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Malinowsky, Kristin. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Ventimiglia, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Zygmunt, Marek. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Jensen, Cristian Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad Nacional Arturo Jauretche; ArgentinaBioScientifica2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120089Bommer, Imke; Juriol, Lorena Vanesa; Muzzio, Damián Oscar; Valeff, Natalin Jimena; Ehrhardt, Jens; et al.; Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth; BioScientifica; Reproduction; 158; 4; 8-2019; 369-3761470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-19-0150info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/view/journals/rep/158/4/REP-19-0150.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:30Zoai:ri.conicet.gov.ar:11336/120089instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:31.0CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
title Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
spellingShingle Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
Bommer, Imke
B cells
Pregnancy
Preterm birth
Amniotic fluid
title_short Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
title_full Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
title_fullStr Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
title_full_unstemmed Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
title_sort Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
dc.creator.none.fl_str_mv Bommer, Imke
Juriol, Lorena Vanesa
Muzzio, Damián Oscar
Valeff, Natalin Jimena
Ehrhardt, Jens
Matzner, Franziska
Ziegler, Katharina
Malinowsky, Kristin
Ventimiglia, Maria Silvia
Zygmunt, Marek
Jensen, Cristian Federico
author Bommer, Imke
author_facet Bommer, Imke
Juriol, Lorena Vanesa
Muzzio, Damián Oscar
Valeff, Natalin Jimena
Ehrhardt, Jens
Matzner, Franziska
Ziegler, Katharina
Malinowsky, Kristin
Ventimiglia, Maria Silvia
Zygmunt, Marek
Jensen, Cristian Federico
author_role author
author2 Juriol, Lorena Vanesa
Muzzio, Damián Oscar
Valeff, Natalin Jimena
Ehrhardt, Jens
Matzner, Franziska
Ziegler, Katharina
Malinowsky, Kristin
Ventimiglia, Maria Silvia
Zygmunt, Marek
Jensen, Cristian Federico
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv B cells
Pregnancy
Preterm birth
Amniotic fluid
topic B cells
Pregnancy
Preterm birth
Amniotic fluid
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The amniotic fluid provides mechanical protection and immune defense against pathogens to the fetus. Indeed, components of the innate and adaptive immunity, including B cells, have been described in the amniotic fluid. However, limited information concerning phenotype and functionality of amniotic fluid B cells is available. Hence, we aimed to perform a full phenotypical and functional characterization of amniotic fluid B cells in normal pregnancy and in a mouse model of preterm birth. Phenotypic analysis depicted the presence of two populations of amniotic fluid B cells: an immature population, resembling B1 progenitor cells and a more mature population. Further isolation and in vitro co-culture with a bone marrow stroma cell line demonstrated the capacity of the immature B cells to mature. This was further supported by spontaneous production of IgM, a feature of the B1 B cell sub-population. An additional in vitro stimulation with lipopolysaccharide induced the activation of amniotic fluid B cells as well as the production of pro and anti-inflammatory cytokines. Furthermore, amniotic fluid B cells were expanded in the acute phase of LPS-induced preterm birth. Overall our data add new insight not only on the phenotype and developmental stage of the amniotic fluid B1 B cells but especially on their functionality. This provides important information for a better understanding of their role within the amniotic fluid as immunological protective barrier, especially with regard to intraamniotic infection and preterm birth.
Fil: Bommer, Imke. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Juriol, Lorena Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Muzzio, Damián Oscar. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Valeff, Natalin Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Ehrhardt, Jens. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Matzner, Franziska. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Ziegler, Katharina. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Malinowsky, Kristin. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Ventimiglia, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Zygmunt, Marek. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Jensen, Cristian Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad Nacional Arturo Jauretche; Argentina
description The amniotic fluid provides mechanical protection and immune defense against pathogens to the fetus. Indeed, components of the innate and adaptive immunity, including B cells, have been described in the amniotic fluid. However, limited information concerning phenotype and functionality of amniotic fluid B cells is available. Hence, we aimed to perform a full phenotypical and functional characterization of amniotic fluid B cells in normal pregnancy and in a mouse model of preterm birth. Phenotypic analysis depicted the presence of two populations of amniotic fluid B cells: an immature population, resembling B1 progenitor cells and a more mature population. Further isolation and in vitro co-culture with a bone marrow stroma cell line demonstrated the capacity of the immature B cells to mature. This was further supported by spontaneous production of IgM, a feature of the B1 B cell sub-population. An additional in vitro stimulation with lipopolysaccharide induced the activation of amniotic fluid B cells as well as the production of pro and anti-inflammatory cytokines. Furthermore, amniotic fluid B cells were expanded in the acute phase of LPS-induced preterm birth. Overall our data add new insight not only on the phenotype and developmental stage of the amniotic fluid B1 B cells but especially on their functionality. This provides important information for a better understanding of their role within the amniotic fluid as immunological protective barrier, especially with regard to intraamniotic infection and preterm birth.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/120089
Bommer, Imke; Juriol, Lorena Vanesa; Muzzio, Damián Oscar; Valeff, Natalin Jimena; Ehrhardt, Jens; et al.; Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth; BioScientifica; Reproduction; 158; 4; 8-2019; 369-376
1470-1626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/120089
identifier_str_mv Bommer, Imke; Juriol, Lorena Vanesa; Muzzio, Damián Oscar; Valeff, Natalin Jimena; Ehrhardt, Jens; et al.; Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth; BioScientifica; Reproduction; 158; 4; 8-2019; 369-376
1470-1626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-19-0150
info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/view/journals/rep/158/4/REP-19-0150.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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