Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
- Autores
- Bommer, Imke; Juriol, Lorena Vanesa; Muzzio, Damián Oscar; Valeff, Natalin Jimena; Ehrhardt, Jens; Matzner, Franziska; Ziegler, Katharina; Malinowsky, Kristin; Ventimiglia, Maria Silvia; Zygmunt, Marek; Jensen, Cristian Federico
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The amniotic fluid provides mechanical protection and immune defense against pathogens to the fetus. Indeed, components of the innate and adaptive immunity, including B cells, have been described in the amniotic fluid. However, limited information concerning phenotype and functionality of amniotic fluid B cells is available. Hence, we aimed to perform a full phenotypical and functional characterization of amniotic fluid B cells in normal pregnancy and in a mouse model of preterm birth. Phenotypic analysis depicted the presence of two populations of amniotic fluid B cells: an immature population, resembling B1 progenitor cells and a more mature population. Further isolation and in vitro co-culture with a bone marrow stroma cell line demonstrated the capacity of the immature B cells to mature. This was further supported by spontaneous production of IgM, a feature of the B1 B cell sub-population. An additional in vitro stimulation with lipopolysaccharide induced the activation of amniotic fluid B cells as well as the production of pro and anti-inflammatory cytokines. Furthermore, amniotic fluid B cells were expanded in the acute phase of LPS-induced preterm birth. Overall our data add new insight not only on the phenotype and developmental stage of the amniotic fluid B1 B cells but especially on their functionality. This provides important information for a better understanding of their role within the amniotic fluid as immunological protective barrier, especially with regard to intraamniotic infection and preterm birth.
Fil: Bommer, Imke. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Juriol, Lorena Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Muzzio, Damián Oscar. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Valeff, Natalin Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Ehrhardt, Jens. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Matzner, Franziska. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Ziegler, Katharina. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Malinowsky, Kristin. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Ventimiglia, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Zygmunt, Marek. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);
Fil: Jensen, Cristian Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad Nacional Arturo Jauretche; Argentina - Materia
-
B cells
Pregnancy
Preterm birth
Amniotic fluid - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/120089
Ver los metadatos del registro completo
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Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birthBommer, ImkeJuriol, Lorena VanesaMuzzio, Damián OscarValeff, Natalin JimenaEhrhardt, JensMatzner, FranziskaZiegler, KatharinaMalinowsky, KristinVentimiglia, Maria SilviaZygmunt, MarekJensen, Cristian FedericoB cellsPregnancyPreterm birthAmniotic fluidhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The amniotic fluid provides mechanical protection and immune defense against pathogens to the fetus. Indeed, components of the innate and adaptive immunity, including B cells, have been described in the amniotic fluid. However, limited information concerning phenotype and functionality of amniotic fluid B cells is available. Hence, we aimed to perform a full phenotypical and functional characterization of amniotic fluid B cells in normal pregnancy and in a mouse model of preterm birth. Phenotypic analysis depicted the presence of two populations of amniotic fluid B cells: an immature population, resembling B1 progenitor cells and a more mature population. Further isolation and in vitro co-culture with a bone marrow stroma cell line demonstrated the capacity of the immature B cells to mature. This was further supported by spontaneous production of IgM, a feature of the B1 B cell sub-population. An additional in vitro stimulation with lipopolysaccharide induced the activation of amniotic fluid B cells as well as the production of pro and anti-inflammatory cytokines. Furthermore, amniotic fluid B cells were expanded in the acute phase of LPS-induced preterm birth. Overall our data add new insight not only on the phenotype and developmental stage of the amniotic fluid B1 B cells but especially on their functionality. This provides important information for a better understanding of their role within the amniotic fluid as immunological protective barrier, especially with regard to intraamniotic infection and preterm birth.Fil: Bommer, Imke. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Juriol, Lorena Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Muzzio, Damián Oscar. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Valeff, Natalin Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Ehrhardt, Jens. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Matzner, Franziska. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Ziegler, Katharina. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Malinowsky, Kristin. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Ventimiglia, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Zygmunt, Marek. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Jensen, Cristian Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad Nacional Arturo Jauretche; ArgentinaBioScientifica2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120089Bommer, Imke; Juriol, Lorena Vanesa; Muzzio, Damián Oscar; Valeff, Natalin Jimena; Ehrhardt, Jens; et al.; Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth; BioScientifica; Reproduction; 158; 4; 8-2019; 369-3761470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-19-0150info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/view/journals/rep/158/4/REP-19-0150.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:34Zoai:ri.conicet.gov.ar:11336/120089instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:34.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth |
title |
Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth |
spellingShingle |
Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth Bommer, Imke B cells Pregnancy Preterm birth Amniotic fluid |
title_short |
Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth |
title_full |
Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth |
title_fullStr |
Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth |
title_full_unstemmed |
Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth |
title_sort |
Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth |
dc.creator.none.fl_str_mv |
Bommer, Imke Juriol, Lorena Vanesa Muzzio, Damián Oscar Valeff, Natalin Jimena Ehrhardt, Jens Matzner, Franziska Ziegler, Katharina Malinowsky, Kristin Ventimiglia, Maria Silvia Zygmunt, Marek Jensen, Cristian Federico |
author |
Bommer, Imke |
author_facet |
Bommer, Imke Juriol, Lorena Vanesa Muzzio, Damián Oscar Valeff, Natalin Jimena Ehrhardt, Jens Matzner, Franziska Ziegler, Katharina Malinowsky, Kristin Ventimiglia, Maria Silvia Zygmunt, Marek Jensen, Cristian Federico |
author_role |
author |
author2 |
Juriol, Lorena Vanesa Muzzio, Damián Oscar Valeff, Natalin Jimena Ehrhardt, Jens Matzner, Franziska Ziegler, Katharina Malinowsky, Kristin Ventimiglia, Maria Silvia Zygmunt, Marek Jensen, Cristian Federico |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
B cells Pregnancy Preterm birth Amniotic fluid |
topic |
B cells Pregnancy Preterm birth Amniotic fluid |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The amniotic fluid provides mechanical protection and immune defense against pathogens to the fetus. Indeed, components of the innate and adaptive immunity, including B cells, have been described in the amniotic fluid. However, limited information concerning phenotype and functionality of amniotic fluid B cells is available. Hence, we aimed to perform a full phenotypical and functional characterization of amniotic fluid B cells in normal pregnancy and in a mouse model of preterm birth. Phenotypic analysis depicted the presence of two populations of amniotic fluid B cells: an immature population, resembling B1 progenitor cells and a more mature population. Further isolation and in vitro co-culture with a bone marrow stroma cell line demonstrated the capacity of the immature B cells to mature. This was further supported by spontaneous production of IgM, a feature of the B1 B cell sub-population. An additional in vitro stimulation with lipopolysaccharide induced the activation of amniotic fluid B cells as well as the production of pro and anti-inflammatory cytokines. Furthermore, amniotic fluid B cells were expanded in the acute phase of LPS-induced preterm birth. Overall our data add new insight not only on the phenotype and developmental stage of the amniotic fluid B1 B cells but especially on their functionality. This provides important information for a better understanding of their role within the amniotic fluid as immunological protective barrier, especially with regard to intraamniotic infection and preterm birth. Fil: Bommer, Imke. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG); Fil: Juriol, Lorena Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Muzzio, Damián Oscar. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG); Fil: Valeff, Natalin Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Ehrhardt, Jens. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG); Fil: Matzner, Franziska. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG); Fil: Ziegler, Katharina. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG); Fil: Malinowsky, Kristin. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG); Fil: Ventimiglia, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Zygmunt, Marek. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG); Fil: Jensen, Cristian Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad Nacional Arturo Jauretche; Argentina |
description |
The amniotic fluid provides mechanical protection and immune defense against pathogens to the fetus. Indeed, components of the innate and adaptive immunity, including B cells, have been described in the amniotic fluid. However, limited information concerning phenotype and functionality of amniotic fluid B cells is available. Hence, we aimed to perform a full phenotypical and functional characterization of amniotic fluid B cells in normal pregnancy and in a mouse model of preterm birth. Phenotypic analysis depicted the presence of two populations of amniotic fluid B cells: an immature population, resembling B1 progenitor cells and a more mature population. Further isolation and in vitro co-culture with a bone marrow stroma cell line demonstrated the capacity of the immature B cells to mature. This was further supported by spontaneous production of IgM, a feature of the B1 B cell sub-population. An additional in vitro stimulation with lipopolysaccharide induced the activation of amniotic fluid B cells as well as the production of pro and anti-inflammatory cytokines. Furthermore, amniotic fluid B cells were expanded in the acute phase of LPS-induced preterm birth. Overall our data add new insight not only on the phenotype and developmental stage of the amniotic fluid B1 B cells but especially on their functionality. This provides important information for a better understanding of their role within the amniotic fluid as immunological protective barrier, especially with regard to intraamniotic infection and preterm birth. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/120089 Bommer, Imke; Juriol, Lorena Vanesa; Muzzio, Damián Oscar; Valeff, Natalin Jimena; Ehrhardt, Jens; et al.; Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth; BioScientifica; Reproduction; 158; 4; 8-2019; 369-376 1470-1626 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/120089 |
identifier_str_mv |
Bommer, Imke; Juriol, Lorena Vanesa; Muzzio, Damián Oscar; Valeff, Natalin Jimena; Ehrhardt, Jens; et al.; Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth; BioScientifica; Reproduction; 158; 4; 8-2019; 369-376 1470-1626 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-19-0150 info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/view/journals/rep/158/4/REP-19-0150.xml |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioScientifica |
publisher.none.fl_str_mv |
BioScientifica |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842269167802122240 |
score |
13.13397 |