Bleomycin-induced trans lipid formation in cell membranes and in liposome models

Autores
Cort, Aysegul; Ozben, Tomris; Sansone, Anna; Barata Vallejo, Sebastian; Chatgilialoglu, Chryssostomos; Ferreri, Carla
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cell cultures of NTera-2 cells incubated with bleomycin and liposomes as biomimetic models of cell membranes were used for examining some novel aspects of drug-metal induced reactivity with unsaturated lipids under oxidative conditions. In cell cultures, bleomycin was found for the first time to cause the formation of trans fatty acids. The chemical basis of this transformation was ascertained by liposome experiments, using bleomycin-iron complexes in the presence of thiol as a reducing agent that by incubation at 37 °C gave rise to the thiyl radical-catalysed double bond isomerisation of membrane phospholipids. The effect of oxygen and reagent concentrations on the reaction outcome was studied. An interesting scenario of free radical reactivity is proposed, which can be relevant for understanding the role of membrane lipids in antitumoral treatments and drug carrier interaction.
Fil: Cort, Aysegul. Akdeniz University. Faculty of Medicine; Turquía. Consiglio Nazionale delle Ricerche; Italia
Fil: Ozben, Tomris. Akdeniz University. Faculty of Medicine; Turquía
Fil: Sansone, Anna. Consiglio Nazionale delle Ricerche; Italia
Fil: Barata Vallejo, Sebastian. Consiglio Nazionale delle Ricerche; Italia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chatgilialoglu, Chryssostomos. Demokritos National Centre For Scientific Research; Grecia
Fil: Ferreri, Carla. Consiglio Nazionale delle Ricerche; Italia
Materia
Biomimetics
Fatty acids
Free radicals
Membrane lipids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37421
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bleomycin-induced trans lipid formation in cell membranes and in liposome modelsCort, AysegulOzben, TomrisSansone, AnnaBarata Vallejo, SebastianChatgilialoglu, ChryssostomosFerreri, CarlaBiomimeticsFatty acidsFree radicalsMembrane lipidshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Cell cultures of NTera-2 cells incubated with bleomycin and liposomes as biomimetic models of cell membranes were used for examining some novel aspects of drug-metal induced reactivity with unsaturated lipids under oxidative conditions. In cell cultures, bleomycin was found for the first time to cause the formation of trans fatty acids. The chemical basis of this transformation was ascertained by liposome experiments, using bleomycin-iron complexes in the presence of thiol as a reducing agent that by incubation at 37 °C gave rise to the thiyl radical-catalysed double bond isomerisation of membrane phospholipids. The effect of oxygen and reagent concentrations on the reaction outcome was studied. An interesting scenario of free radical reactivity is proposed, which can be relevant for understanding the role of membrane lipids in antitumoral treatments and drug carrier interaction.Fil: Cort, Aysegul. Akdeniz University. Faculty of Medicine; Turquía. Consiglio Nazionale delle Ricerche; ItaliaFil: Ozben, Tomris. Akdeniz University. Faculty of Medicine; TurquíaFil: Sansone, Anna. Consiglio Nazionale delle Ricerche; ItaliaFil: Barata Vallejo, Sebastian. Consiglio Nazionale delle Ricerche; Italia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chatgilialoglu, Chryssostomos. Demokritos National Centre For Scientific Research; GreciaFil: Ferreri, Carla. Consiglio Nazionale delle Ricerche; ItaliaRoyal Society of Chemistry2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37421Cort, Aysegul; Ozben, Tomris; Sansone, Anna; Barata Vallejo, Sebastian; Chatgilialoglu, Chryssostomos; et al.; Bleomycin-induced trans lipid formation in cell membranes and in liposome models; Royal Society of Chemistry; Organic & Biomolecular Chemistry; 13; 4; 1-2015; 1100-11051477-0520CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c4ob01924einfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2015/OB/C4OB01924Einfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:29:43Zoai:ri.conicet.gov.ar:11336/37421instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:29:44.023CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bleomycin-induced trans lipid formation in cell membranes and in liposome models
title Bleomycin-induced trans lipid formation in cell membranes and in liposome models
spellingShingle Bleomycin-induced trans lipid formation in cell membranes and in liposome models
Cort, Aysegul
Biomimetics
Fatty acids
Free radicals
Membrane lipids
title_short Bleomycin-induced trans lipid formation in cell membranes and in liposome models
title_full Bleomycin-induced trans lipid formation in cell membranes and in liposome models
title_fullStr Bleomycin-induced trans lipid formation in cell membranes and in liposome models
title_full_unstemmed Bleomycin-induced trans lipid formation in cell membranes and in liposome models
title_sort Bleomycin-induced trans lipid formation in cell membranes and in liposome models
dc.creator.none.fl_str_mv Cort, Aysegul
Ozben, Tomris
Sansone, Anna
Barata Vallejo, Sebastian
Chatgilialoglu, Chryssostomos
Ferreri, Carla
author Cort, Aysegul
author_facet Cort, Aysegul
Ozben, Tomris
Sansone, Anna
Barata Vallejo, Sebastian
Chatgilialoglu, Chryssostomos
Ferreri, Carla
author_role author
author2 Ozben, Tomris
Sansone, Anna
Barata Vallejo, Sebastian
Chatgilialoglu, Chryssostomos
Ferreri, Carla
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biomimetics
Fatty acids
Free radicals
Membrane lipids
topic Biomimetics
Fatty acids
Free radicals
Membrane lipids
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cell cultures of NTera-2 cells incubated with bleomycin and liposomes as biomimetic models of cell membranes were used for examining some novel aspects of drug-metal induced reactivity with unsaturated lipids under oxidative conditions. In cell cultures, bleomycin was found for the first time to cause the formation of trans fatty acids. The chemical basis of this transformation was ascertained by liposome experiments, using bleomycin-iron complexes in the presence of thiol as a reducing agent that by incubation at 37 °C gave rise to the thiyl radical-catalysed double bond isomerisation of membrane phospholipids. The effect of oxygen and reagent concentrations on the reaction outcome was studied. An interesting scenario of free radical reactivity is proposed, which can be relevant for understanding the role of membrane lipids in antitumoral treatments and drug carrier interaction.
Fil: Cort, Aysegul. Akdeniz University. Faculty of Medicine; Turquía. Consiglio Nazionale delle Ricerche; Italia
Fil: Ozben, Tomris. Akdeniz University. Faculty of Medicine; Turquía
Fil: Sansone, Anna. Consiglio Nazionale delle Ricerche; Italia
Fil: Barata Vallejo, Sebastian. Consiglio Nazionale delle Ricerche; Italia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chatgilialoglu, Chryssostomos. Demokritos National Centre For Scientific Research; Grecia
Fil: Ferreri, Carla. Consiglio Nazionale delle Ricerche; Italia
description Cell cultures of NTera-2 cells incubated with bleomycin and liposomes as biomimetic models of cell membranes were used for examining some novel aspects of drug-metal induced reactivity with unsaturated lipids under oxidative conditions. In cell cultures, bleomycin was found for the first time to cause the formation of trans fatty acids. The chemical basis of this transformation was ascertained by liposome experiments, using bleomycin-iron complexes in the presence of thiol as a reducing agent that by incubation at 37 °C gave rise to the thiyl radical-catalysed double bond isomerisation of membrane phospholipids. The effect of oxygen and reagent concentrations on the reaction outcome was studied. An interesting scenario of free radical reactivity is proposed, which can be relevant for understanding the role of membrane lipids in antitumoral treatments and drug carrier interaction.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37421
Cort, Aysegul; Ozben, Tomris; Sansone, Anna; Barata Vallejo, Sebastian; Chatgilialoglu, Chryssostomos; et al.; Bleomycin-induced trans lipid formation in cell membranes and in liposome models; Royal Society of Chemistry; Organic & Biomolecular Chemistry; 13; 4; 1-2015; 1100-1105
1477-0520
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37421
identifier_str_mv Cort, Aysegul; Ozben, Tomris; Sansone, Anna; Barata Vallejo, Sebastian; Chatgilialoglu, Chryssostomos; et al.; Bleomycin-induced trans lipid formation in cell membranes and in liposome models; Royal Society of Chemistry; Organic & Biomolecular Chemistry; 13; 4; 1-2015; 1100-1105
1477-0520
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c4ob01924e
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2015/OB/C4OB01924E
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781885212000256
score 12.982451

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