Different serotonin type 3 subunit can coassemble into heteromeric receptors

Autores
Corradi, Jeremias; Mazzarini Dimarco, Albano; Bouzat, Cecilia Beatriz
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
5-HT3 receptors are the only serotonin (5-HT) receptors that belong to the Cys-loop receptor family. They mediate fast excitatory transmission in central and peripheral nervous system. Five different subunits (A-E) have been identified in humans. The A subunit is able to form functional homomeric receptors (5-HT3A), and it can also combine with the B subunit to form heteromeric receptors (5-HT3AB). To evaluate the capability of the C, D and E subunits to combine with the A subunit to form heteromeric receptors we performed single-channel and macroscopic recordings. After expression of the A subunit, we recorded single-channel openings with an amplitude corresponding to the 5-HT3A receptor. However, when this subunit was expressed with one of the C-E subunits, opening events with different amplitudes were detected, thus confirming the expression of heteromeric receptors. From macroscopic currents we determined that the EC50 values for 5-HT were statistically different when homomeric or heteromeric receptors were expressed. Taking together, our results demonstrate that all the 5-HT3 subunits can combine with the A subunit to form heteromeric receptors. In-silico studies provided insights into the contribution of the different subunits to the 5-HT binding site. The functional characterization of different heteromeric 5-HT3 receptors will contribute to the development of selective therapies targeting this receptor family
Fil: Corradi, Jeremias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mazzarini Dimarco, Albano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias
Córdoba
Argentina
Sociedad Argentina de Investigación en Neurociencias.
Materia
SEROTONIN
HETEROMERIC
TYPE 3
SUBUNITS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230286
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/230286
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Different serotonin type 3 subunit can coassemble into heteromeric receptorsCorradi, JeremiasMazzarini Dimarco, AlbanoBouzat, Cecilia BeatrizSEROTONINHETEROMERICTYPE 3SUBUNITShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/15-HT3 receptors are the only serotonin (5-HT) receptors that belong to the Cys-loop receptor family. They mediate fast excitatory transmission in central and peripheral nervous system. Five different subunits (A-E) have been identified in humans. The A subunit is able to form functional homomeric receptors (5-HT3A), and it can also combine with the B subunit to form heteromeric receptors (5-HT3AB). To evaluate the capability of the C, D and E subunits to combine with the A subunit to form heteromeric receptors we performed single-channel and macroscopic recordings. After expression of the A subunit, we recorded single-channel openings with an amplitude corresponding to the 5-HT3A receptor. However, when this subunit was expressed with one of the C-E subunits, opening events with different amplitudes were detected, thus confirming the expression of heteromeric receptors. From macroscopic currents we determined that the EC50 values for 5-HT were statistically different when homomeric or heteromeric receptors were expressed. Taking together, our results demonstrate that all the 5-HT3 subunits can combine with the A subunit to form heteromeric receptors. In-silico studies provided insights into the contribution of the different subunits to the 5-HT binding site. The functional characterization of different heteromeric 5-HT3 receptors will contribute to the development of selective therapies targeting this receptor familyFil: Corradi, Jeremias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Mazzarini Dimarco, Albano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXXXIII Congreso Anual de la Sociedad Argentina de Investigación en NeurocienciasCórdobaArgentinaSociedad Argentina de Investigación en Neurociencias.Sociedad Argentina de Investigación en Neurociencias2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230286Different serotonin type 3 subunit can coassemble into heteromeric receptors; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 315-315CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/congresos-san-2/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:11Zoai:ri.conicet.gov.ar:11336/230286instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:11.852CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Different serotonin type 3 subunit can coassemble into heteromeric receptors
title Different serotonin type 3 subunit can coassemble into heteromeric receptors
spellingShingle Different serotonin type 3 subunit can coassemble into heteromeric receptors
Corradi, Jeremias
SEROTONIN
HETEROMERIC
TYPE 3
SUBUNITS
title_short Different serotonin type 3 subunit can coassemble into heteromeric receptors
title_full Different serotonin type 3 subunit can coassemble into heteromeric receptors
title_fullStr Different serotonin type 3 subunit can coassemble into heteromeric receptors
title_full_unstemmed Different serotonin type 3 subunit can coassemble into heteromeric receptors
title_sort Different serotonin type 3 subunit can coassemble into heteromeric receptors
dc.creator.none.fl_str_mv Corradi, Jeremias
Mazzarini Dimarco, Albano
Bouzat, Cecilia Beatriz
author Corradi, Jeremias
author_facet Corradi, Jeremias
Mazzarini Dimarco, Albano
Bouzat, Cecilia Beatriz
author_role author
author2 Mazzarini Dimarco, Albano
Bouzat, Cecilia Beatriz
author2_role author
author
dc.subject.none.fl_str_mv SEROTONIN
HETEROMERIC
TYPE 3
SUBUNITS
topic SEROTONIN
HETEROMERIC
TYPE 3
SUBUNITS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv 5-HT3 receptors are the only serotonin (5-HT) receptors that belong to the Cys-loop receptor family. They mediate fast excitatory transmission in central and peripheral nervous system. Five different subunits (A-E) have been identified in humans. The A subunit is able to form functional homomeric receptors (5-HT3A), and it can also combine with the B subunit to form heteromeric receptors (5-HT3AB). To evaluate the capability of the C, D and E subunits to combine with the A subunit to form heteromeric receptors we performed single-channel and macroscopic recordings. After expression of the A subunit, we recorded single-channel openings with an amplitude corresponding to the 5-HT3A receptor. However, when this subunit was expressed with one of the C-E subunits, opening events with different amplitudes were detected, thus confirming the expression of heteromeric receptors. From macroscopic currents we determined that the EC50 values for 5-HT were statistically different when homomeric or heteromeric receptors were expressed. Taking together, our results demonstrate that all the 5-HT3 subunits can combine with the A subunit to form heteromeric receptors. In-silico studies provided insights into the contribution of the different subunits to the 5-HT binding site. The functional characterization of different heteromeric 5-HT3 receptors will contribute to the development of selective therapies targeting this receptor family
Fil: Corradi, Jeremias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mazzarini Dimarco, Albano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias
Córdoba
Argentina
Sociedad Argentina de Investigación en Neurociencias.
description 5-HT3 receptors are the only serotonin (5-HT) receptors that belong to the Cys-loop receptor family. They mediate fast excitatory transmission in central and peripheral nervous system. Five different subunits (A-E) have been identified in humans. The A subunit is able to form functional homomeric receptors (5-HT3A), and it can also combine with the B subunit to form heteromeric receptors (5-HT3AB). To evaluate the capability of the C, D and E subunits to combine with the A subunit to form heteromeric receptors we performed single-channel and macroscopic recordings. After expression of the A subunit, we recorded single-channel openings with an amplitude corresponding to the 5-HT3A receptor. However, when this subunit was expressed with one of the C-E subunits, opening events with different amplitudes were detected, thus confirming the expression of heteromeric receptors. From macroscopic currents we determined that the EC50 values for 5-HT were statistically different when homomeric or heteromeric receptors were expressed. Taking together, our results demonstrate that all the 5-HT3 subunits can combine with the A subunit to form heteromeric receptors. In-silico studies provided insights into the contribution of the different subunits to the 5-HT binding site. The functional characterization of different heteromeric 5-HT3 receptors will contribute to the development of selective therapies targeting this receptor family
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230286
Different serotonin type 3 subunit can coassemble into heteromeric receptors; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 315-315
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230286
identifier_str_mv Different serotonin type 3 subunit can coassemble into heteromeric receptors; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 315-315
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/congresos-san-2/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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