Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants

Autores
Bargmann, Bastiaan O. R.; Laxalt, Ana Maria; Riet, Bas ter; van Schooten, Bas; Merquiol, Emmanuelle; Testerink, Christa; Haring, Michel A.; Bartels, Dorothea; Munnik, Teun
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High salinity and drought have received much attention because they severely affect crop production worldwide. Analysis and comprehension of the plant's response to excessive salt and dehydration will aid in the development of stress-tolerant crop varieties. Signal transduction lies at the basis of the response to these stresses, and numerous signaling pathways have been implicated. Here, we provide further evidence for the involvement of phospholipase D (PLD) in the plant's response to high salinity and dehydration. A tomato ( Lycopersicon esculentum ) α -class PLD, LePLD α 1, is transcriptionally up-regulated and activated in cell suspension cultures treated with salt. Gene silencing revealed that this PLD is indeed involved in the salt-induced phosphatidic acid production, but not exclusively. Genetically modifi ed tomato plants with reduced LePLD α 1 protein levels did not reveal altered salt tolerance. In Arabidopsis ( Arabidopsis thaliana ), both AtPLD α 1 and AtPLD δ were found to be activated in response to salt stress. Moreover, pldα1 and pldδ single and double knock-out mutants exhibited enhanced sensitivity to high salinity stress in a plate assay. Furthermore, we show that both PLDs are activated upon dehydration and the knock-out mutants are hypersensitive to hyperosmotic stress, displaying strongly reduced growth.
Fil: Bargmann, Bastiaan O. R.. Universiteit van Amsterdam; Países Bajos
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universiteit van Amsterdam; Países Bajos
Fil: Riet, Bas ter. Universiteit van Amsterdam; Países Bajos
Fil: van Schooten, Bas. Universiteit van Amsterdam; Países Bajos
Fil: Merquiol, Emmanuelle. Vrije Universiteit Amsterdam.; Países Bajos
Fil: Testerink, Christa. Universiteit van Amsterdam; Países Bajos
Fil: Haring, Michel A.. Universiteit van Amsterdam; Países Bajos
Fil: Bartels, Dorothea. Universität Bonn; Alemania
Fil: Munnik, Teun. Universiteit van Amsterdam; Países Bajos
Materia
phospholipase D
salt stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105127
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/105127
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multiple PLDs Required for High Salinity and Water Deficit Tolerance in PlantsBargmann, Bastiaan O. R.Laxalt, Ana MariaRiet, Bas tervan Schooten, BasMerquiol, EmmanuelleTesterink, ChristaHaring, Michel A.Bartels, DorotheaMunnik, Teunphospholipase Dsalt stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1High salinity and drought have received much attention because they severely affect crop production worldwide. Analysis and comprehension of the plant's response to excessive salt and dehydration will aid in the development of stress-tolerant crop varieties. Signal transduction lies at the basis of the response to these stresses, and numerous signaling pathways have been implicated. Here, we provide further evidence for the involvement of phospholipase D (PLD) in the plant's response to high salinity and dehydration. A tomato ( Lycopersicon esculentum ) α -class PLD, LePLD α 1, is transcriptionally up-regulated and activated in cell suspension cultures treated with salt. Gene silencing revealed that this PLD is indeed involved in the salt-induced phosphatidic acid production, but not exclusively. Genetically modifi ed tomato plants with reduced LePLD α 1 protein levels did not reveal altered salt tolerance. In Arabidopsis ( Arabidopsis thaliana ), both AtPLD α 1 and AtPLD δ were found to be activated in response to salt stress. Moreover, pldα1 and pldδ single and double knock-out mutants exhibited enhanced sensitivity to high salinity stress in a plate assay. Furthermore, we show that both PLDs are activated upon dehydration and the knock-out mutants are hypersensitive to hyperosmotic stress, displaying strongly reduced growth.Fil: Bargmann, Bastiaan O. R.. Universiteit van Amsterdam; Países BajosFil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universiteit van Amsterdam; Países BajosFil: Riet, Bas ter. Universiteit van Amsterdam; Países BajosFil: van Schooten, Bas. Universiteit van Amsterdam; Países BajosFil: Merquiol, Emmanuelle. Vrije Universiteit Amsterdam.; Países BajosFil: Testerink, Christa. Universiteit van Amsterdam; Países BajosFil: Haring, Michel A.. Universiteit van Amsterdam; Países BajosFil: Bartels, Dorothea. Universität Bonn; AlemaniaFil: Munnik, Teun. Universiteit van Amsterdam; Países BajosOxford University Press2008-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105127Bargmann, Bastiaan O. R.; Laxalt, Ana Maria; Riet, Bas ter; van Schooten, Bas; Merquiol, Emmanuelle; et al.; Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants; Oxford University Press; Plant And Cell Physiology; 50; 1; 11-2008; 78-890032-0781CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/pcp/pcn173info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/pcp/article/50/1/78/1849628info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:40:47Zoai:ri.conicet.gov.ar:11336/105127instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:40:47.652CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
title Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
spellingShingle Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
Bargmann, Bastiaan O. R.
phospholipase D
salt stress
title_short Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
title_full Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
title_fullStr Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
title_full_unstemmed Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
title_sort Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
dc.creator.none.fl_str_mv Bargmann, Bastiaan O. R.
Laxalt, Ana Maria
Riet, Bas ter
van Schooten, Bas
Merquiol, Emmanuelle
Testerink, Christa
Haring, Michel A.
Bartels, Dorothea
Munnik, Teun
author Bargmann, Bastiaan O. R.
author_facet Bargmann, Bastiaan O. R.
Laxalt, Ana Maria
Riet, Bas ter
van Schooten, Bas
Merquiol, Emmanuelle
Testerink, Christa
Haring, Michel A.
Bartels, Dorothea
Munnik, Teun
author_role author
author2 Laxalt, Ana Maria
Riet, Bas ter
van Schooten, Bas
Merquiol, Emmanuelle
Testerink, Christa
Haring, Michel A.
Bartels, Dorothea
Munnik, Teun
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv phospholipase D
salt stress
topic phospholipase D
salt stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv High salinity and drought have received much attention because they severely affect crop production worldwide. Analysis and comprehension of the plant's response to excessive salt and dehydration will aid in the development of stress-tolerant crop varieties. Signal transduction lies at the basis of the response to these stresses, and numerous signaling pathways have been implicated. Here, we provide further evidence for the involvement of phospholipase D (PLD) in the plant's response to high salinity and dehydration. A tomato ( Lycopersicon esculentum ) α -class PLD, LePLD α 1, is transcriptionally up-regulated and activated in cell suspension cultures treated with salt. Gene silencing revealed that this PLD is indeed involved in the salt-induced phosphatidic acid production, but not exclusively. Genetically modifi ed tomato plants with reduced LePLD α 1 protein levels did not reveal altered salt tolerance. In Arabidopsis ( Arabidopsis thaliana ), both AtPLD α 1 and AtPLD δ were found to be activated in response to salt stress. Moreover, pldα1 and pldδ single and double knock-out mutants exhibited enhanced sensitivity to high salinity stress in a plate assay. Furthermore, we show that both PLDs are activated upon dehydration and the knock-out mutants are hypersensitive to hyperosmotic stress, displaying strongly reduced growth.
Fil: Bargmann, Bastiaan O. R.. Universiteit van Amsterdam; Países Bajos
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universiteit van Amsterdam; Países Bajos
Fil: Riet, Bas ter. Universiteit van Amsterdam; Países Bajos
Fil: van Schooten, Bas. Universiteit van Amsterdam; Países Bajos
Fil: Merquiol, Emmanuelle. Vrije Universiteit Amsterdam.; Países Bajos
Fil: Testerink, Christa. Universiteit van Amsterdam; Países Bajos
Fil: Haring, Michel A.. Universiteit van Amsterdam; Países Bajos
Fil: Bartels, Dorothea. Universität Bonn; Alemania
Fil: Munnik, Teun. Universiteit van Amsterdam; Países Bajos
description High salinity and drought have received much attention because they severely affect crop production worldwide. Analysis and comprehension of the plant's response to excessive salt and dehydration will aid in the development of stress-tolerant crop varieties. Signal transduction lies at the basis of the response to these stresses, and numerous signaling pathways have been implicated. Here, we provide further evidence for the involvement of phospholipase D (PLD) in the plant's response to high salinity and dehydration. A tomato ( Lycopersicon esculentum ) α -class PLD, LePLD α 1, is transcriptionally up-regulated and activated in cell suspension cultures treated with salt. Gene silencing revealed that this PLD is indeed involved in the salt-induced phosphatidic acid production, but not exclusively. Genetically modifi ed tomato plants with reduced LePLD α 1 protein levels did not reveal altered salt tolerance. In Arabidopsis ( Arabidopsis thaliana ), both AtPLD α 1 and AtPLD δ were found to be activated in response to salt stress. Moreover, pldα1 and pldδ single and double knock-out mutants exhibited enhanced sensitivity to high salinity stress in a plate assay. Furthermore, we show that both PLDs are activated upon dehydration and the knock-out mutants are hypersensitive to hyperosmotic stress, displaying strongly reduced growth.
publishDate 2008
dc.date.none.fl_str_mv 2008-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105127
Bargmann, Bastiaan O. R.; Laxalt, Ana Maria; Riet, Bas ter; van Schooten, Bas; Merquiol, Emmanuelle; et al.; Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants; Oxford University Press; Plant And Cell Physiology; 50; 1; 11-2008; 78-89
0032-0781
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105127
identifier_str_mv Bargmann, Bastiaan O. R.; Laxalt, Ana Maria; Riet, Bas ter; van Schooten, Bas; Merquiol, Emmanuelle; et al.; Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants; Oxford University Press; Plant And Cell Physiology; 50; 1; 11-2008; 78-89
0032-0781
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/pcp/pcn173
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/pcp/article/50/1/78/1849628
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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