Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry

Autores
Bigatti, Gregorio; Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Penchaszadeh, Pablo Enrique; Castro Vazquez, Alfredo Juan
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Embryo morphology, feeding mechanism and changes in composition of the egg capsule content during development (intracapsular fluids and embryos) were studied in Odontocymbiola magellanica from newly spawned egg capsules to the pre-hatching juvenile stage. Changes in embryo morphology and behavior are presented, based on observations and micrographs of living specimens and scanning electron microscopy. The arrangement of velar cilia and athrocytes and shell gland location and development differed markedly from other studied caenogastropods. Embryo ingestion of intracapsular fluid was promoted by velar ciliary currents at least from the early veliger stage, while feeding by grazing on the inner membranous layer of the egg capsule was rarely observed until juveniles were about to hatch. The main growth of embryos occurred during the veliger stages. A significant nutritional investment in egg capsules, as compared with other South American volutids was observed. Nutrition from proteins seemed to predominate at the expense of a high molecular weight fraction (>220 kDa). Calcium concentration in the intracapsular fluid remained constant during development, but notably, the total intracapsular content (i.e., the amount contained in both fluid and embryos) increased 3-fold, which may be explained by extraction from the egg capsule magnesium-rich calcite cover, or alternatively, by uptake of calcium from the surrounding sea water. Ammonia, a major end-product of nitrogen metabolism in marine invertebrates, was present in both embryos and intracapsular fluid, from which it may easily diffuse to the surrounding sea water through the egg capsule wall. Our results on embryo morphology, development and biochemical changes provide useful comparative data for evolutionary and developmental studies in the Volutidae as well as in other caenogastropods.
Fil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Cientiíâ­ficas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Cientiíâ­ficas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "dr. M. Burgos"; Argentina
Fil: Penchaszadeh, Pablo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales; Argentina
Fil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
Materia
Caenogastropoda
Athrocytes
Developmental Traits
Egg-Capsules
Embryo Nutrition
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10402
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10402
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistryBigatti, GregorioGiraud Billoud, Maximiliano GermanVega, Israel AníbalPenchaszadeh, Pablo EnriqueCastro Vazquez, Alfredo JuanCaenogastropodaAthrocytesDevelopmental TraitsEgg-CapsulesEmbryo Nutritionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Embryo morphology, feeding mechanism and changes in composition of the egg capsule content during development (intracapsular fluids and embryos) were studied in Odontocymbiola magellanica from newly spawned egg capsules to the pre-hatching juvenile stage. Changes in embryo morphology and behavior are presented, based on observations and micrographs of living specimens and scanning electron microscopy. The arrangement of velar cilia and athrocytes and shell gland location and development differed markedly from other studied caenogastropods. Embryo ingestion of intracapsular fluid was promoted by velar ciliary currents at least from the early veliger stage, while feeding by grazing on the inner membranous layer of the egg capsule was rarely observed until juveniles were about to hatch. The main growth of embryos occurred during the veliger stages. A significant nutritional investment in egg capsules, as compared with other South American volutids was observed. Nutrition from proteins seemed to predominate at the expense of a high molecular weight fraction (>220 kDa). Calcium concentration in the intracapsular fluid remained constant during development, but notably, the total intracapsular content (i.e., the amount contained in both fluid and embryos) increased 3-fold, which may be explained by extraction from the egg capsule magnesium-rich calcite cover, or alternatively, by uptake of calcium from the surrounding sea water. Ammonia, a major end-product of nitrogen metabolism in marine invertebrates, was present in both embryos and intracapsular fluid, from which it may easily diffuse to the surrounding sea water through the egg capsule wall. Our results on embryo morphology, development and biochemical changes provide useful comparative data for evolutionary and developmental studies in the Volutidae as well as in other caenogastropods.Fil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Cientiíâ­ficas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; ArgentinaFil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Cientiíâ­ficas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "dr. M. Burgos"; ArgentinaFil: Penchaszadeh, Pablo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales; ArgentinaFil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; ArgentinaElsevier Gmbh2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10402Bigatti, Gregorio; Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Penchaszadeh, Pablo Enrique; Castro Vazquez, Alfredo Juan; Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry; Elsevier Gmbh; Zoologischer Anzeiger; 253; 5; 6-2014; 372-3810044-5231enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0044523114000199info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcz.2014.03.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:00:42Zoai:ri.conicet.gov.ar:11336/10402instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:00:42.642CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry
title Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry
spellingShingle Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry
Bigatti, Gregorio
Caenogastropoda
Athrocytes
Developmental Traits
Egg-Capsules
Embryo Nutrition
title_short Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry
title_full Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry
title_fullStr Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry
title_full_unstemmed Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry
title_sort Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry
dc.creator.none.fl_str_mv Bigatti, Gregorio
Giraud Billoud, Maximiliano German
Vega, Israel Aníbal
Penchaszadeh, Pablo Enrique
Castro Vazquez, Alfredo Juan
author Bigatti, Gregorio
author_facet Bigatti, Gregorio
Giraud Billoud, Maximiliano German
Vega, Israel Aníbal
Penchaszadeh, Pablo Enrique
Castro Vazquez, Alfredo Juan
author_role author
author2 Giraud Billoud, Maximiliano German
Vega, Israel Aníbal
Penchaszadeh, Pablo Enrique
Castro Vazquez, Alfredo Juan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Caenogastropoda
Athrocytes
Developmental Traits
Egg-Capsules
Embryo Nutrition
topic Caenogastropoda
Athrocytes
Developmental Traits
Egg-Capsules
Embryo Nutrition
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Embryo morphology, feeding mechanism and changes in composition of the egg capsule content during development (intracapsular fluids and embryos) were studied in Odontocymbiola magellanica from newly spawned egg capsules to the pre-hatching juvenile stage. Changes in embryo morphology and behavior are presented, based on observations and micrographs of living specimens and scanning electron microscopy. The arrangement of velar cilia and athrocytes and shell gland location and development differed markedly from other studied caenogastropods. Embryo ingestion of intracapsular fluid was promoted by velar ciliary currents at least from the early veliger stage, while feeding by grazing on the inner membranous layer of the egg capsule was rarely observed until juveniles were about to hatch. The main growth of embryos occurred during the veliger stages. A significant nutritional investment in egg capsules, as compared with other South American volutids was observed. Nutrition from proteins seemed to predominate at the expense of a high molecular weight fraction (>220 kDa). Calcium concentration in the intracapsular fluid remained constant during development, but notably, the total intracapsular content (i.e., the amount contained in both fluid and embryos) increased 3-fold, which may be explained by extraction from the egg capsule magnesium-rich calcite cover, or alternatively, by uptake of calcium from the surrounding sea water. Ammonia, a major end-product of nitrogen metabolism in marine invertebrates, was present in both embryos and intracapsular fluid, from which it may easily diffuse to the surrounding sea water through the egg capsule wall. Our results on embryo morphology, development and biochemical changes provide useful comparative data for evolutionary and developmental studies in the Volutidae as well as in other caenogastropods.
Fil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Cientiíâ­ficas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Cientiíâ­ficas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "dr. M. Burgos"; Argentina
Fil: Penchaszadeh, Pablo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales; Argentina
Fil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
description Embryo morphology, feeding mechanism and changes in composition of the egg capsule content during development (intracapsular fluids and embryos) were studied in Odontocymbiola magellanica from newly spawned egg capsules to the pre-hatching juvenile stage. Changes in embryo morphology and behavior are presented, based on observations and micrographs of living specimens and scanning electron microscopy. The arrangement of velar cilia and athrocytes and shell gland location and development differed markedly from other studied caenogastropods. Embryo ingestion of intracapsular fluid was promoted by velar ciliary currents at least from the early veliger stage, while feeding by grazing on the inner membranous layer of the egg capsule was rarely observed until juveniles were about to hatch. The main growth of embryos occurred during the veliger stages. A significant nutritional investment in egg capsules, as compared with other South American volutids was observed. Nutrition from proteins seemed to predominate at the expense of a high molecular weight fraction (>220 kDa). Calcium concentration in the intracapsular fluid remained constant during development, but notably, the total intracapsular content (i.e., the amount contained in both fluid and embryos) increased 3-fold, which may be explained by extraction from the egg capsule magnesium-rich calcite cover, or alternatively, by uptake of calcium from the surrounding sea water. Ammonia, a major end-product of nitrogen metabolism in marine invertebrates, was present in both embryos and intracapsular fluid, from which it may easily diffuse to the surrounding sea water through the egg capsule wall. Our results on embryo morphology, development and biochemical changes provide useful comparative data for evolutionary and developmental studies in the Volutidae as well as in other caenogastropods.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10402
Bigatti, Gregorio; Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Penchaszadeh, Pablo Enrique; Castro Vazquez, Alfredo Juan; Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry; Elsevier Gmbh; Zoologischer Anzeiger; 253; 5; 6-2014; 372-381
0044-5231
url http://hdl.handle.net/11336/10402
identifier_str_mv Bigatti, Gregorio; Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Penchaszadeh, Pablo Enrique; Castro Vazquez, Alfredo Juan; Embryonic development in the Patagonian red snail Odontocymbiola magellanica (Neogastropoda: Volutidae): morphology and biochemistry; Elsevier Gmbh; Zoologischer Anzeiger; 253; 5; 6-2014; 372-381
0044-5231
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0044523114000199
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcz.2014.03.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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