Effects of photooxidation on membrane integrity in Salix nigra seeds

Autores
Roqueiro, Gonzalo; Facorro, Graciela B.; Huarte, Mónica G.; Rubín de Celis, Emilio; Garcia, Carlos Fernando; Maldonado, Sara Beatriz; Maroder, Horacio
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation.
Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Facorro, Graciela B.. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; Argentina
Fil: Huarte, Mónica G.. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; Argentina
Fil: Rubín de Celis, Emilio. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia, Carlos Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Maldonado, Sara Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maroder, Horacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Materia
Embryo Membrane Integrity
Free Radicals
Orthodox Seed
Photooxidation
Salicaceae Seeds
Salix Nigra
Seed Chlorophyll
Seed Lipid Peroxidation
Thylakoids
Willow Seeds
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/70634
Acceder
id CONICETDig_81f6fa1e7bbc3eefabaae291a8b9d0fe
oai_identifier_str oai:ri.conicet.gov.ar:11336/70634
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of photooxidation on membrane integrity in Salix nigra seedsRoqueiro, GonzaloFacorro, Graciela B.Huarte, Mónica G.Rubín de Celis, EmilioGarcia, Carlos FernandoMaldonado, Sara BeatrizMaroder, HoracioEmbryo Membrane IntegrityFree RadicalsOrthodox SeedPhotooxidationSalicaceae SeedsSalix NigraSeed ChlorophyllSeed Lipid PeroxidationThylakoidsWillow Seedshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation.Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; ArgentinaFil: Facorro, Graciela B.. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; ArgentinaFil: Huarte, Mónica G.. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; ArgentinaFil: Rubín de Celis, Emilio. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Carlos Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Maldonado, Sara Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maroder, Horacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaOxford University Press2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/70634Roqueiro, Gonzalo; Facorro, Graciela B.; Huarte, Mónica G.; Rubín de Celis, Emilio; Garcia, Carlos Fernando; et al.; Effects of photooxidation on membrane integrity in Salix nigra seeds; Oxford University Press; Annals of Botany; 105; 6; 6-2010; 1027-10340305-7364CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/aob/mcq067info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/aob/article/105/6/1027/94387info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:27Zoai:ri.conicet.gov.ar:11336/70634instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:27.792CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of photooxidation on membrane integrity in Salix nigra seeds
title Effects of photooxidation on membrane integrity in Salix nigra seeds
spellingShingle Effects of photooxidation on membrane integrity in Salix nigra seeds
Roqueiro, Gonzalo
Embryo Membrane Integrity
Free Radicals
Orthodox Seed
Photooxidation
Salicaceae Seeds
Salix Nigra
Seed Chlorophyll
Seed Lipid Peroxidation
Thylakoids
Willow Seeds
title_short Effects of photooxidation on membrane integrity in Salix nigra seeds
title_full Effects of photooxidation on membrane integrity in Salix nigra seeds
title_fullStr Effects of photooxidation on membrane integrity in Salix nigra seeds
title_full_unstemmed Effects of photooxidation on membrane integrity in Salix nigra seeds
title_sort Effects of photooxidation on membrane integrity in Salix nigra seeds
dc.creator.none.fl_str_mv Roqueiro, Gonzalo
Facorro, Graciela B.
Huarte, Mónica G.
Rubín de Celis, Emilio
Garcia, Carlos Fernando
Maldonado, Sara Beatriz
Maroder, Horacio
author Roqueiro, Gonzalo
author_facet Roqueiro, Gonzalo
Facorro, Graciela B.
Huarte, Mónica G.
Rubín de Celis, Emilio
Garcia, Carlos Fernando
Maldonado, Sara Beatriz
Maroder, Horacio
author_role author
author2 Facorro, Graciela B.
Huarte, Mónica G.
Rubín de Celis, Emilio
Garcia, Carlos Fernando
Maldonado, Sara Beatriz
Maroder, Horacio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Embryo Membrane Integrity
Free Radicals
Orthodox Seed
Photooxidation
Salicaceae Seeds
Salix Nigra
Seed Chlorophyll
Seed Lipid Peroxidation
Thylakoids
Willow Seeds
topic Embryo Membrane Integrity
Free Radicals
Orthodox Seed
Photooxidation
Salicaceae Seeds
Salix Nigra
Seed Chlorophyll
Seed Lipid Peroxidation
Thylakoids
Willow Seeds
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation.
Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Facorro, Graciela B.. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; Argentina
Fil: Huarte, Mónica G.. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; Argentina
Fil: Rubín de Celis, Emilio. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia, Carlos Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Maldonado, Sara Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maroder, Horacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
description Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/70634
Roqueiro, Gonzalo; Facorro, Graciela B.; Huarte, Mónica G.; Rubín de Celis, Emilio; Garcia, Carlos Fernando; et al.; Effects of photooxidation on membrane integrity in Salix nigra seeds; Oxford University Press; Annals of Botany; 105; 6; 6-2010; 1027-1034
0305-7364
CONICET Digital
CONICET
url http://hdl.handle.net/11336/70634
identifier_str_mv Roqueiro, Gonzalo; Facorro, Graciela B.; Huarte, Mónica G.; Rubín de Celis, Emilio; Garcia, Carlos Fernando; et al.; Effects of photooxidation on membrane integrity in Salix nigra seeds; Oxford University Press; Annals of Botany; 105; 6; 6-2010; 1027-1034
0305-7364
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/aob/mcq067
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/aob/article/105/6/1027/94387
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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