Effects of photooxidation on membrane integrity in Salix nigra seeds
- Autores
- Roqueiro, G.; Facorro, G.B.; Huarte, M.G.; Rubín De Celis, E.; García, F.; Maldonado, S.; Maroder, H.
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010.
Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Ann. Bot. 2010;105(6):1027-1034
- Materia
-
Embryo membrane integrity
Free radicals
Orthodox seed
Photooxidation
Salicaceae seeds
Salix nigra
Seed chlorophyll
Seed lipid peroxidation
Thylakoids
Willow seeds
chlorophyll
oxygen
chlorophyll
chloroplast
deciduous tree
desiccation
embryo
fatty acid
free radical
germination
light intensity
lipid
liquid chromatography
membrane
permeability
photooxidation
seed
spectroscopy
temperature effect
tolerance
transmission electron microscopy
adaptation
article
light
membrane
metabolism
methodology
nuclear magnetic resonance spectroscopy
oxidation reduction reaction
photochemistry
physiology
plant seed
radiation exposure
willow
Adaptation, Physiological
Chlorophyll
Light
Magnetic Resonance Spectroscopy
Membranes
Oxidation-Reduction
Oxygen
Photochemistry
Salix
Seeds
Salicaceae
Salix
Salix nigra
Salicaceae
Salix
Salix nigra - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_03057364_v105_n6_p1027_Roqueiro
Ver los metadatos del registro completo
id |
BDUBAFCEN_bd12a2f38bddd5d7219390d5f0d532ff |
---|---|
oai_identifier_str |
paperaa:paper_03057364_v105_n6_p1027_Roqueiro |
network_acronym_str |
BDUBAFCEN |
repository_id_str |
1896 |
network_name_str |
Biblioteca Digital (UBA-FCEN) |
spelling |
Effects of photooxidation on membrane integrity in Salix nigra seedsRoqueiro, G.Facorro, G.B.Huarte, M.G.Rubín De Celis, E.García, F.Maldonado, S.Maroder, H.Embryo membrane integrityFree radicalsOrthodox seedPhotooxidationSalicaceae seedsSalix nigraSeed chlorophyllSeed lipid peroxidationThylakoidsWillow seedschlorophylloxygenchlorophyllchloroplastdeciduous treedesiccationembryofatty acidfree radicalgerminationlight intensitylipidliquid chromatographymembranepermeabilityphotooxidationseedspectroscopytemperature effecttolerancetransmission electron microscopyadaptationarticlelightmembranemetabolismmethodologynuclear magnetic resonance spectroscopyoxidation reduction reactionphotochemistryphysiologyplant seedradiation exposurewillowAdaptation, PhysiologicalChlorophyllLightMagnetic Resonance SpectroscopyMembranesOxidation-ReductionOxygenPhotochemistrySalixSeedsSalicaceaeSalixSalix nigraSalicaceaeSalixSalix nigraBackground and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010.Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_03057364_v105_n6_p1027_RoqueiroAnn. Bot. 2010;105(6):1027-1034reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:03Zpaperaa:paper_03057364_v105_n6_p1027_RoqueiroInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:04.627Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
title |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
spellingShingle |
Effects of photooxidation on membrane integrity in Salix nigra seeds Roqueiro, G. Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds chlorophyll oxygen chlorophyll chloroplast deciduous tree desiccation embryo fatty acid free radical germination light intensity lipid liquid chromatography membrane permeability photooxidation seed spectroscopy temperature effect tolerance transmission electron microscopy adaptation article light membrane metabolism methodology nuclear magnetic resonance spectroscopy oxidation reduction reaction photochemistry physiology plant seed radiation exposure willow Adaptation, Physiological Chlorophyll Light Magnetic Resonance Spectroscopy Membranes Oxidation-Reduction Oxygen Photochemistry Salix Seeds Salicaceae Salix Salix nigra Salicaceae Salix Salix nigra |
title_short |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
title_full |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
title_fullStr |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
title_full_unstemmed |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
title_sort |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
dc.creator.none.fl_str_mv |
Roqueiro, G. Facorro, G.B. Huarte, M.G. Rubín De Celis, E. García, F. Maldonado, S. Maroder, H. |
author |
Roqueiro, G. |
author_facet |
Roqueiro, G. Facorro, G.B. Huarte, M.G. Rubín De Celis, E. García, F. Maldonado, S. Maroder, H. |
author_role |
author |
author2 |
Facorro, G.B. Huarte, M.G. Rubín De Celis, E. García, F. Maldonado, S. Maroder, H. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds chlorophyll oxygen chlorophyll chloroplast deciduous tree desiccation embryo fatty acid free radical germination light intensity lipid liquid chromatography membrane permeability photooxidation seed spectroscopy temperature effect tolerance transmission electron microscopy adaptation article light membrane metabolism methodology nuclear magnetic resonance spectroscopy oxidation reduction reaction photochemistry physiology plant seed radiation exposure willow Adaptation, Physiological Chlorophyll Light Magnetic Resonance Spectroscopy Membranes Oxidation-Reduction Oxygen Photochemistry Salix Seeds Salicaceae Salix Salix nigra Salicaceae Salix Salix nigra |
topic |
Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds chlorophyll oxygen chlorophyll chloroplast deciduous tree desiccation embryo fatty acid free radical germination light intensity lipid liquid chromatography membrane permeability photooxidation seed spectroscopy temperature effect tolerance transmission electron microscopy adaptation article light membrane metabolism methodology nuclear magnetic resonance spectroscopy oxidation reduction reaction photochemistry physiology plant seed radiation exposure willow Adaptation, Physiological Chlorophyll Light Magnetic Resonance Spectroscopy Membranes Oxidation-Reduction Oxygen Photochemistry Salix Seeds Salicaceae Salix Salix nigra Salicaceae Salix Salix nigra |
dc.description.none.fl_txt_mv |
Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010. Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_03057364_v105_n6_p1027_Roqueiro |
url |
http://hdl.handle.net/20.500.12110/paper_03057364_v105_n6_p1027_Roqueiro |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Ann. Bot. 2010;105(6):1027-1034 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
_version_ |
1844618738582683648 |
score |
13.070432 |