Effects of photooxidation on membrane integrity in Salix nigra seeds

Autores
Roqueiro, G.; Facorro, G.B.; Huarte, M.G.; Rubín De Celis, E.; García, F.; Maldonado, S.; Maroder, H.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010.
Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Ann. Bot. 2010;105(6):1027-1034
Materia
Embryo membrane integrity
Free radicals
Orthodox seed
Photooxidation
Salicaceae seeds
Salix nigra
Seed chlorophyll
Seed lipid peroxidation
Thylakoids
Willow seeds
chlorophyll
oxygen
chlorophyll
chloroplast
deciduous tree
desiccation
embryo
fatty acid
free radical
germination
light intensity
lipid
liquid chromatography
membrane
permeability
photooxidation
seed
spectroscopy
temperature effect
tolerance
transmission electron microscopy
adaptation
article
light
membrane
metabolism
methodology
nuclear magnetic resonance spectroscopy
oxidation reduction reaction
photochemistry
physiology
plant seed
radiation exposure
willow
Adaptation, Physiological
Chlorophyll
Light
Magnetic Resonance Spectroscopy
Membranes
Oxidation-Reduction
Oxygen
Photochemistry
Salix
Seeds
Salicaceae
Salix
Salix nigra
Salicaceae
Salix
Salix nigra
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_03057364_v105_n6_p1027_Roqueiro

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oai_identifier_str paperaa:paper_03057364_v105_n6_p1027_Roqueiro
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Effects of photooxidation on membrane integrity in Salix nigra seedsRoqueiro, G.Facorro, G.B.Huarte, M.G.Rubín De Celis, E.García, F.Maldonado, S.Maroder, H.Embryo membrane integrityFree radicalsOrthodox seedPhotooxidationSalicaceae seedsSalix nigraSeed chlorophyllSeed lipid peroxidationThylakoidsWillow seedschlorophylloxygenchlorophyllchloroplastdeciduous treedesiccationembryofatty acidfree radicalgerminationlight intensitylipidliquid chromatographymembranepermeabilityphotooxidationseedspectroscopytemperature effecttolerancetransmission electron microscopyadaptationarticlelightmembranemetabolismmethodologynuclear magnetic resonance spectroscopyoxidation reduction reactionphotochemistryphysiologyplant seedradiation exposurewillowAdaptation, PhysiologicalChlorophyllLightMagnetic Resonance SpectroscopyMembranesOxidation-ReductionOxygenPhotochemistrySalixSeedsSalicaceaeSalixSalix nigraSalicaceaeSalixSalix nigraBackground and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010.Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_03057364_v105_n6_p1027_RoqueiroAnn. Bot. 2010;105(6):1027-1034reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:03Zpaperaa:paper_03057364_v105_n6_p1027_RoqueiroInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:04.627Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Effects of photooxidation on membrane integrity in Salix nigra seeds
title Effects of photooxidation on membrane integrity in Salix nigra seeds
spellingShingle Effects of photooxidation on membrane integrity in Salix nigra seeds
Roqueiro, G.
Embryo membrane integrity
Free radicals
Orthodox seed
Photooxidation
Salicaceae seeds
Salix nigra
Seed chlorophyll
Seed lipid peroxidation
Thylakoids
Willow seeds
chlorophyll
oxygen
chlorophyll
chloroplast
deciduous tree
desiccation
embryo
fatty acid
free radical
germination
light intensity
lipid
liquid chromatography
membrane
permeability
photooxidation
seed
spectroscopy
temperature effect
tolerance
transmission electron microscopy
adaptation
article
light
membrane
metabolism
methodology
nuclear magnetic resonance spectroscopy
oxidation reduction reaction
photochemistry
physiology
plant seed
radiation exposure
willow
Adaptation, Physiological
Chlorophyll
Light
Magnetic Resonance Spectroscopy
Membranes
Oxidation-Reduction
Oxygen
Photochemistry
Salix
Seeds
Salicaceae
Salix
Salix nigra
Salicaceae
Salix
Salix nigra
title_short Effects of photooxidation on membrane integrity in Salix nigra seeds
title_full Effects of photooxidation on membrane integrity in Salix nigra seeds
title_fullStr Effects of photooxidation on membrane integrity in Salix nigra seeds
title_full_unstemmed Effects of photooxidation on membrane integrity in Salix nigra seeds
title_sort Effects of photooxidation on membrane integrity in Salix nigra seeds
dc.creator.none.fl_str_mv Roqueiro, G.
Facorro, G.B.
Huarte, M.G.
Rubín De Celis, E.
García, F.
Maldonado, S.
Maroder, H.
author Roqueiro, G.
author_facet Roqueiro, G.
Facorro, G.B.
Huarte, M.G.
Rubín De Celis, E.
García, F.
Maldonado, S.
Maroder, H.
author_role author
author2 Facorro, G.B.
Huarte, M.G.
Rubín De Celis, E.
García, F.
Maldonado, S.
Maroder, H.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Embryo membrane integrity
Free radicals
Orthodox seed
Photooxidation
Salicaceae seeds
Salix nigra
Seed chlorophyll
Seed lipid peroxidation
Thylakoids
Willow seeds
chlorophyll
oxygen
chlorophyll
chloroplast
deciduous tree
desiccation
embryo
fatty acid
free radical
germination
light intensity
lipid
liquid chromatography
membrane
permeability
photooxidation
seed
spectroscopy
temperature effect
tolerance
transmission electron microscopy
adaptation
article
light
membrane
metabolism
methodology
nuclear magnetic resonance spectroscopy
oxidation reduction reaction
photochemistry
physiology
plant seed
radiation exposure
willow
Adaptation, Physiological
Chlorophyll
Light
Magnetic Resonance Spectroscopy
Membranes
Oxidation-Reduction
Oxygen
Photochemistry
Salix
Seeds
Salicaceae
Salix
Salix nigra
Salicaceae
Salix
Salix nigra
topic Embryo membrane integrity
Free radicals
Orthodox seed
Photooxidation
Salicaceae seeds
Salix nigra
Seed chlorophyll
Seed lipid peroxidation
Thylakoids
Willow seeds
chlorophyll
oxygen
chlorophyll
chloroplast
deciduous tree
desiccation
embryo
fatty acid
free radical
germination
light intensity
lipid
liquid chromatography
membrane
permeability
photooxidation
seed
spectroscopy
temperature effect
tolerance
transmission electron microscopy
adaptation
article
light
membrane
metabolism
methodology
nuclear magnetic resonance spectroscopy
oxidation reduction reaction
photochemistry
physiology
plant seed
radiation exposure
willow
Adaptation, Physiological
Chlorophyll
Light
Magnetic Resonance Spectroscopy
Membranes
Oxidation-Reduction
Oxygen
Photochemistry
Salix
Seeds
Salicaceae
Salix
Salix nigra
Salicaceae
Salix
Salix nigra
dc.description.none.fl_txt_mv Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010.
Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_03057364_v105_n6_p1027_Roqueiro
url http://hdl.handle.net/20.500.12110/paper_03057364_v105_n6_p1027_Roqueiro
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Ann. Bot. 2010;105(6):1027-1034
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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