In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells

Autores
Irazoqui, Ana Paula; González Pardo, María Verónica; Buitrago, Claudia Graciela; Bühler, Kathrin; Russo de Boland, Ana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
We have previously shown that Solanum glaucophyllum leaves extract (SGE) enriched with 1a,25(OH)2D3-glucosides exhibits at least equal or greater effects on early myoblast differentiation as synthetic 1a,25(OH)2D3 thus being an effective substitute to promote muscle growth. Recently, we reported the effects of SGE compared to synthetic 1a,25(OH)2D3 in the regulation of mitogen activated protein kinases (MAPKs), genes involved on the differentiation of murine skeletal muscle cells C2C12, and their role on myotube formation. Since MAP kinase phosphatases (MKPs) deficiency impairs skeletal muscle regeneration and exacerbates muscular dystrophy, we further studied the effects of SGE on MKP1 and MKP5 which function to specifically dephosphorylate and inactivate MAPKs. SGE did not statistically modified MKP-1 and MKP-5 protein levels although basal changes were observed. MKP-1 protein levels remained unchanged at 1, 24 and 48 hours and decreased at 6 and 72 hours. MKP-5 protein levels increased at differentiation onset (24-72 h). We found that SGE significantly induced biphasic changes in phosphatase activity, decreased at 1 and 24 hours (which correlates with the pro-differentiation cell arrest previously reported), and increased at 6 hours. We also investigated the effects of SGE on Akt-mTOR signaling during the differentiation phase. SGE, decreased Akt phosphorylation after 5-day and increased its phosphorylation after 7-day of treatment. However, SGE did not induced changes in mTOR phosphorylation during time (3-7-day). Following with this approach, we investigated in vivo effects of SGE on skeletal muscle function in mice. Results from the first in vivo pilot test, have evidenced that 2 mg SGE/kg BW for 30 days did not show statically significant differences in phosphorus, calcium and glucose parameters from SGE groups compared to control. However, SGE leads to an increase in soleus muscle VDR protein expression in line with a significant decrease in 25(OH)D3 in blood serum while lower SGE doses did not change biochemical parameters measured in serum or protein content in mice muscle. These results contribute to elucidate the mechanism of action of the herbal active form of vitamin D when used as substitute to promote muscle growth
Fil: Irazoqui, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Buitrago, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Bühler, Kathrin. Herbonis Animal Health GmbH; Suiza
Fil: Russo de Boland, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
2020 Annual Meeting of the American Society for Bone and Mineral Research
Virtual
Estados Unidos
The American Society for Bone and Mineral Research
Materia
1a,25(OH)3 VITAMINA D
MÚSCULO ESQUELÉTICO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/274949
Acceder
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network_name_str CONICET Digital (CONICET)
spelling In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cellsIrazoqui, Ana PaulaGonzález Pardo, María VerónicaBuitrago, Claudia GracielaBühler, KathrinRusso de Boland, Ana1a,25(OH)3 VITAMINA DMÚSCULO ESQUELÉTICOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have previously shown that Solanum glaucophyllum leaves extract (SGE) enriched with 1a,25(OH)2D3-glucosides exhibits at least equal or greater effects on early myoblast differentiation as synthetic 1a,25(OH)2D3 thus being an effective substitute to promote muscle growth. Recently, we reported the effects of SGE compared to synthetic 1a,25(OH)2D3 in the regulation of mitogen activated protein kinases (MAPKs), genes involved on the differentiation of murine skeletal muscle cells C2C12, and their role on myotube formation. Since MAP kinase phosphatases (MKPs) deficiency impairs skeletal muscle regeneration and exacerbates muscular dystrophy, we further studied the effects of SGE on MKP1 and MKP5 which function to specifically dephosphorylate and inactivate MAPKs. SGE did not statistically modified MKP-1 and MKP-5 protein levels although basal changes were observed. MKP-1 protein levels remained unchanged at 1, 24 and 48 hours and decreased at 6 and 72 hours. MKP-5 protein levels increased at differentiation onset (24-72 h). We found that SGE significantly induced biphasic changes in phosphatase activity, decreased at 1 and 24 hours (which correlates with the pro-differentiation cell arrest previously reported), and increased at 6 hours. We also investigated the effects of SGE on Akt-mTOR signaling during the differentiation phase. SGE, decreased Akt phosphorylation after 5-day and increased its phosphorylation after 7-day of treatment. However, SGE did not induced changes in mTOR phosphorylation during time (3-7-day). Following with this approach, we investigated in vivo effects of SGE on skeletal muscle function in mice. Results from the first in vivo pilot test, have evidenced that 2 mg SGE/kg BW for 30 days did not show statically significant differences in phosphorus, calcium and glucose parameters from SGE groups compared to control. However, SGE leads to an increase in soleus muscle VDR protein expression in line with a significant decrease in 25(OH)D3 in blood serum while lower SGE doses did not change biochemical parameters measured in serum or protein content in mice muscle. These results contribute to elucidate the mechanism of action of the herbal active form of vitamin D when used as substitute to promote muscle growthFil: Irazoqui, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Buitrago, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Bühler, Kathrin. Herbonis Animal Health GmbH; SuizaFil: Russo de Boland, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina2020 Annual Meeting of the American Society for Bone and Mineral ResearchVirtualEstados UnidosThe American Society for Bone and Mineral ResearchWiley2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/274949In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells; 2020 Annual Meeting of the American Society for Bone and Mineral Research; Virtual; Estados Unidos; 2020; 139 -1390884-0431CONICET DigitalCONICETenghttps://www.asbmr.org/meetings/past-ASBMR-meetingsinfo:eu-repo/semantics/altIdentifier/url/https://www.asbmr.org/2020-abstractsInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T09:46:08Zoai:ri.conicet.gov.ar:11336/274949instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 09:46:08.333CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells
title In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells
spellingShingle In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells
Irazoqui, Ana Paula
1a,25(OH)3 VITAMINA D
MÚSCULO ESQUELÉTICO
title_short In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells
title_full In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells
title_fullStr In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells
title_full_unstemmed In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells
title_sort In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells
dc.creator.none.fl_str_mv Irazoqui, Ana Paula
González Pardo, María Verónica
Buitrago, Claudia Graciela
Bühler, Kathrin
Russo de Boland, Ana
author Irazoqui, Ana Paula
author_facet Irazoqui, Ana Paula
González Pardo, María Verónica
Buitrago, Claudia Graciela
Bühler, Kathrin
Russo de Boland, Ana
author_role author
author2 González Pardo, María Verónica
Buitrago, Claudia Graciela
Bühler, Kathrin
Russo de Boland, Ana
author2_role author
author
author
author
dc.subject.none.fl_str_mv 1a,25(OH)3 VITAMINA D
MÚSCULO ESQUELÉTICO
topic 1a,25(OH)3 VITAMINA D
MÚSCULO ESQUELÉTICO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have previously shown that Solanum glaucophyllum leaves extract (SGE) enriched with 1a,25(OH)2D3-glucosides exhibits at least equal or greater effects on early myoblast differentiation as synthetic 1a,25(OH)2D3 thus being an effective substitute to promote muscle growth. Recently, we reported the effects of SGE compared to synthetic 1a,25(OH)2D3 in the regulation of mitogen activated protein kinases (MAPKs), genes involved on the differentiation of murine skeletal muscle cells C2C12, and their role on myotube formation. Since MAP kinase phosphatases (MKPs) deficiency impairs skeletal muscle regeneration and exacerbates muscular dystrophy, we further studied the effects of SGE on MKP1 and MKP5 which function to specifically dephosphorylate and inactivate MAPKs. SGE did not statistically modified MKP-1 and MKP-5 protein levels although basal changes were observed. MKP-1 protein levels remained unchanged at 1, 24 and 48 hours and decreased at 6 and 72 hours. MKP-5 protein levels increased at differentiation onset (24-72 h). We found that SGE significantly induced biphasic changes in phosphatase activity, decreased at 1 and 24 hours (which correlates with the pro-differentiation cell arrest previously reported), and increased at 6 hours. We also investigated the effects of SGE on Akt-mTOR signaling during the differentiation phase. SGE, decreased Akt phosphorylation after 5-day and increased its phosphorylation after 7-day of treatment. However, SGE did not induced changes in mTOR phosphorylation during time (3-7-day). Following with this approach, we investigated in vivo effects of SGE on skeletal muscle function in mice. Results from the first in vivo pilot test, have evidenced that 2 mg SGE/kg BW for 30 days did not show statically significant differences in phosphorus, calcium and glucose parameters from SGE groups compared to control. However, SGE leads to an increase in soleus muscle VDR protein expression in line with a significant decrease in 25(OH)D3 in blood serum while lower SGE doses did not change biochemical parameters measured in serum or protein content in mice muscle. These results contribute to elucidate the mechanism of action of the herbal active form of vitamin D when used as substitute to promote muscle growth
Fil: Irazoqui, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Buitrago, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Bühler, Kathrin. Herbonis Animal Health GmbH; Suiza
Fil: Russo de Boland, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
2020 Annual Meeting of the American Society for Bone and Mineral Research
Virtual
Estados Unidos
The American Society for Bone and Mineral Research
description We have previously shown that Solanum glaucophyllum leaves extract (SGE) enriched with 1a,25(OH)2D3-glucosides exhibits at least equal or greater effects on early myoblast differentiation as synthetic 1a,25(OH)2D3 thus being an effective substitute to promote muscle growth. Recently, we reported the effects of SGE compared to synthetic 1a,25(OH)2D3 in the regulation of mitogen activated protein kinases (MAPKs), genes involved on the differentiation of murine skeletal muscle cells C2C12, and their role on myotube formation. Since MAP kinase phosphatases (MKPs) deficiency impairs skeletal muscle regeneration and exacerbates muscular dystrophy, we further studied the effects of SGE on MKP1 and MKP5 which function to specifically dephosphorylate and inactivate MAPKs. SGE did not statistically modified MKP-1 and MKP-5 protein levels although basal changes were observed. MKP-1 protein levels remained unchanged at 1, 24 and 48 hours and decreased at 6 and 72 hours. MKP-5 protein levels increased at differentiation onset (24-72 h). We found that SGE significantly induced biphasic changes in phosphatase activity, decreased at 1 and 24 hours (which correlates with the pro-differentiation cell arrest previously reported), and increased at 6 hours. We also investigated the effects of SGE on Akt-mTOR signaling during the differentiation phase. SGE, decreased Akt phosphorylation after 5-day and increased its phosphorylation after 7-day of treatment. However, SGE did not induced changes in mTOR phosphorylation during time (3-7-day). Following with this approach, we investigated in vivo effects of SGE on skeletal muscle function in mice. Results from the first in vivo pilot test, have evidenced that 2 mg SGE/kg BW for 30 days did not show statically significant differences in phosphorus, calcium and glucose parameters from SGE groups compared to control. However, SGE leads to an increase in soleus muscle VDR protein expression in line with a significant decrease in 25(OH)D3 in blood serum while lower SGE doses did not change biochemical parameters measured in serum or protein content in mice muscle. These results contribute to elucidate the mechanism of action of the herbal active form of vitamin D when used as substitute to promote muscle growth
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/274949
In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells; 2020 Annual Meeting of the American Society for Bone and Mineral Research; Virtual; Estados Unidos; 2020; 139 -139
0884-0431
CONICET Digital
CONICET
url http://hdl.handle.net/11336/274949
identifier_str_mv In vivo effects of 1a,25(OH)2D3-glycosides from Solanum glaucophyllum leaves in mice and skeletal muscle cells; 2020 Annual Meeting of the American Society for Bone and Mineral Research; Virtual; Estados Unidos; 2020; 139 -139
0884-0431
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.asbmr.org/meetings/past-ASBMR-meetings
info:eu-repo/semantics/altIdentifier/url/https://www.asbmr.org/2020-abstracts
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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