Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct

Autores
Kotlar, Catalina Elena; Ponce, Alejandra Graciela; Roura, Sara Ines
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Proteases and proteolytic enzymes constitute one of the most important groups of enzymes and are attracting worldwide attentionin attempts to exploit their physiological and biotechnological applications. In this study, partial purifications and biochemicaland antimicrobial characterizations of a protease from Bacillus cereus spp., originally isolated from fermented cabbage, werecarried out. The crude extract obtained after purification, involving ammonium sulphate precipitation and dialysis, was designatedas a partially purified protease (PPP). The obtained PPP had a specific activity of 0.395?2.539 U/g at 32 °C, with maximumactivities for the fractions precipitated at 60 and 80% ammonium sulphate. The PPP activity ranged between 20 and 55 °C, withan optimum temperature at 40 °C. At 60 °C, the PPP retained more than 30% of its activity. The optimum pH for the PPP wasachieved at pH 9, indicating the alkaline source of the enzyme. Protease production was specifically dependent on the calciumconcentration in the culture medium. Also the robustness of the protease on brewer?s spent grain hydrolysis was demonstrated.This suggests a potential eco-friendly application of the enzyme. Finally, it was found that the PPP inhibited the growth ofEscherichia coli O157:H7. This novel property of the PPP liberated by the B. cereus spp. could provide important future benefitsto industry.
Fil: Kotlar, Catalina Elena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Roura, Sara Ines. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Materia
ANTIMICROBIAL ACTIVITY
PARTIAL PURIFICATION
PROTEASE
BACILLUS CEREUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101700
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproductKotlar, Catalina ElenaPonce, Alejandra GracielaRoura, Sara InesANTIMICROBIAL ACTIVITYPARTIAL PURIFICATIONPROTEASEBACILLUS CEREUShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Proteases and proteolytic enzymes constitute one of the most important groups of enzymes and are attracting worldwide attentionin attempts to exploit their physiological and biotechnological applications. In this study, partial purifications and biochemicaland antimicrobial characterizations of a protease from Bacillus cereus spp., originally isolated from fermented cabbage, werecarried out. The crude extract obtained after purification, involving ammonium sulphate precipitation and dialysis, was designatedas a partially purified protease (PPP). The obtained PPP had a specific activity of 0.395?2.539 U/g at 32 °C, with maximumactivities for the fractions precipitated at 60 and 80% ammonium sulphate. The PPP activity ranged between 20 and 55 °C, withan optimum temperature at 40 °C. At 60 °C, the PPP retained more than 30% of its activity. The optimum pH for the PPP wasachieved at pH 9, indicating the alkaline source of the enzyme. Protease production was specifically dependent on the calciumconcentration in the culture medium. Also the robustness of the protease on brewer?s spent grain hydrolysis was demonstrated.This suggests a potential eco-friendly application of the enzyme. Finally, it was found that the PPP inhibited the growth ofEscherichia coli O157:H7. This novel property of the PPP liberated by the B. cereus spp. could provide important future benefitsto industry.Fil: Kotlar, Catalina Elena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; ArgentinaFil: Roura, Sara Ines. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaInst Brewing2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101700Kotlar, Catalina Elena; Ponce, Alejandra Graciela; Roura, Sara Ines; Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct; Inst Brewing; Journal of The Institute of Brewing; 121; 7-2015; 558-5650046-9750CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jib.257info:eu-repo/semantics/altIdentifier/doi/10.1002/jib.257info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:42:11Zoai:ri.conicet.gov.ar:11336/101700instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:42:11.72CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct
title Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct
spellingShingle Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct
Kotlar, Catalina Elena
ANTIMICROBIAL ACTIVITY
PARTIAL PURIFICATION
PROTEASE
BACILLUS CEREUS
title_short Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct
title_full Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct
title_fullStr Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct
title_full_unstemmed Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct
title_sort Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct
dc.creator.none.fl_str_mv Kotlar, Catalina Elena
Ponce, Alejandra Graciela
Roura, Sara Ines
author Kotlar, Catalina Elena
author_facet Kotlar, Catalina Elena
Ponce, Alejandra Graciela
Roura, Sara Ines
author_role author
author2 Ponce, Alejandra Graciela
Roura, Sara Ines
author2_role author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL ACTIVITY
PARTIAL PURIFICATION
PROTEASE
BACILLUS CEREUS
topic ANTIMICROBIAL ACTIVITY
PARTIAL PURIFICATION
PROTEASE
BACILLUS CEREUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Proteases and proteolytic enzymes constitute one of the most important groups of enzymes and are attracting worldwide attentionin attempts to exploit their physiological and biotechnological applications. In this study, partial purifications and biochemicaland antimicrobial characterizations of a protease from Bacillus cereus spp., originally isolated from fermented cabbage, werecarried out. The crude extract obtained after purification, involving ammonium sulphate precipitation and dialysis, was designatedas a partially purified protease (PPP). The obtained PPP had a specific activity of 0.395?2.539 U/g at 32 °C, with maximumactivities for the fractions precipitated at 60 and 80% ammonium sulphate. The PPP activity ranged between 20 and 55 °C, withan optimum temperature at 40 °C. At 60 °C, the PPP retained more than 30% of its activity. The optimum pH for the PPP wasachieved at pH 9, indicating the alkaline source of the enzyme. Protease production was specifically dependent on the calciumconcentration in the culture medium. Also the robustness of the protease on brewer?s spent grain hydrolysis was demonstrated.This suggests a potential eco-friendly application of the enzyme. Finally, it was found that the PPP inhibited the growth ofEscherichia coli O157:H7. This novel property of the PPP liberated by the B. cereus spp. could provide important future benefitsto industry.
Fil: Kotlar, Catalina Elena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Roura, Sara Ines. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
description Proteases and proteolytic enzymes constitute one of the most important groups of enzymes and are attracting worldwide attentionin attempts to exploit their physiological and biotechnological applications. In this study, partial purifications and biochemicaland antimicrobial characterizations of a protease from Bacillus cereus spp., originally isolated from fermented cabbage, werecarried out. The crude extract obtained after purification, involving ammonium sulphate precipitation and dialysis, was designatedas a partially purified protease (PPP). The obtained PPP had a specific activity of 0.395?2.539 U/g at 32 °C, with maximumactivities for the fractions precipitated at 60 and 80% ammonium sulphate. The PPP activity ranged between 20 and 55 °C, withan optimum temperature at 40 °C. At 60 °C, the PPP retained more than 30% of its activity. The optimum pH for the PPP wasachieved at pH 9, indicating the alkaline source of the enzyme. Protease production was specifically dependent on the calciumconcentration in the culture medium. Also the robustness of the protease on brewer?s spent grain hydrolysis was demonstrated.This suggests a potential eco-friendly application of the enzyme. Finally, it was found that the PPP inhibited the growth ofEscherichia coli O157:H7. This novel property of the PPP liberated by the B. cereus spp. could provide important future benefitsto industry.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101700
Kotlar, Catalina Elena; Ponce, Alejandra Graciela; Roura, Sara Ines; Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct; Inst Brewing; Journal of The Institute of Brewing; 121; 7-2015; 558-565
0046-9750
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101700
identifier_str_mv Kotlar, Catalina Elena; Ponce, Alejandra Graciela; Roura, Sara Ines; Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct; Inst Brewing; Journal of The Institute of Brewing; 121; 7-2015; 558-565
0046-9750
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jib.257
info:eu-repo/semantics/altIdentifier/doi/10.1002/jib.257
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inst Brewing
publisher.none.fl_str_mv Inst Brewing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082922218520576
score 13.22299

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