Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct
- Autores
- Kotlar, Catalina Elena; Ponce, Alejandra Graciela; Roura, Sara Ines
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Proteases and proteolytic enzymes constitute one of the most important groups of enzymes and are attracting worldwide attentionin attempts to exploit their physiological and biotechnological applications. In this study, partial purifications and biochemicaland antimicrobial characterizations of a protease from Bacillus cereus spp., originally isolated from fermented cabbage, werecarried out. The crude extract obtained after purification, involving ammonium sulphate precipitation and dialysis, was designatedas a partially purified protease (PPP). The obtained PPP had a specific activity of 0.395?2.539 U/g at 32 °C, with maximumactivities for the fractions precipitated at 60 and 80% ammonium sulphate. The PPP activity ranged between 20 and 55 °C, withan optimum temperature at 40 °C. At 60 °C, the PPP retained more than 30% of its activity. The optimum pH for the PPP wasachieved at pH 9, indicating the alkaline source of the enzyme. Protease production was specifically dependent on the calciumconcentration in the culture medium. Also the robustness of the protease on brewer?s spent grain hydrolysis was demonstrated.This suggests a potential eco-friendly application of the enzyme. Finally, it was found that the PPP inhibited the growth ofEscherichia coli O157:H7. This novel property of the PPP liberated by the B. cereus spp. could provide important future benefitsto industry.
Fil: Kotlar, Catalina Elena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Roura, Sara Ines. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina - Materia
-
ANTIMICROBIAL ACTIVITY
PARTIAL PURIFICATION
PROTEASE
BACILLUS CEREUS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/101700
Ver los metadatos del registro completo
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spelling |
Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproductKotlar, Catalina ElenaPonce, Alejandra GracielaRoura, Sara InesANTIMICROBIAL ACTIVITYPARTIAL PURIFICATIONPROTEASEBACILLUS CEREUShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Proteases and proteolytic enzymes constitute one of the most important groups of enzymes and are attracting worldwide attentionin attempts to exploit their physiological and biotechnological applications. In this study, partial purifications and biochemicaland antimicrobial characterizations of a protease from Bacillus cereus spp., originally isolated from fermented cabbage, werecarried out. The crude extract obtained after purification, involving ammonium sulphate precipitation and dialysis, was designatedas a partially purified protease (PPP). The obtained PPP had a specific activity of 0.395?2.539 U/g at 32 °C, with maximumactivities for the fractions precipitated at 60 and 80% ammonium sulphate. The PPP activity ranged between 20 and 55 °C, withan optimum temperature at 40 °C. At 60 °C, the PPP retained more than 30% of its activity. The optimum pH for the PPP wasachieved at pH 9, indicating the alkaline source of the enzyme. Protease production was specifically dependent on the calciumconcentration in the culture medium. Also the robustness of the protease on brewer?s spent grain hydrolysis was demonstrated.This suggests a potential eco-friendly application of the enzyme. Finally, it was found that the PPP inhibited the growth ofEscherichia coli O157:H7. This novel property of the PPP liberated by the B. cereus spp. could provide important future benefitsto industry.Fil: Kotlar, Catalina Elena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; ArgentinaFil: Roura, Sara Ines. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaInst Brewing2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101700Kotlar, Catalina Elena; Ponce, Alejandra Graciela; Roura, Sara Ines; Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct; Inst Brewing; Journal of The Institute of Brewing; 121; 7-2015; 558-5650046-9750CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jib.257info:eu-repo/semantics/altIdentifier/doi/10.1002/jib.257info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:16Zoai:ri.conicet.gov.ar:11336/101700instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:16.333CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct |
title |
Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct |
spellingShingle |
Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct Kotlar, Catalina Elena ANTIMICROBIAL ACTIVITY PARTIAL PURIFICATION PROTEASE BACILLUS CEREUS |
title_short |
Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct |
title_full |
Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct |
title_fullStr |
Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct |
title_full_unstemmed |
Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct |
title_sort |
Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct |
dc.creator.none.fl_str_mv |
Kotlar, Catalina Elena Ponce, Alejandra Graciela Roura, Sara Ines |
author |
Kotlar, Catalina Elena |
author_facet |
Kotlar, Catalina Elena Ponce, Alejandra Graciela Roura, Sara Ines |
author_role |
author |
author2 |
Ponce, Alejandra Graciela Roura, Sara Ines |
author2_role |
author author |
dc.subject.none.fl_str_mv |
ANTIMICROBIAL ACTIVITY PARTIAL PURIFICATION PROTEASE BACILLUS CEREUS |
topic |
ANTIMICROBIAL ACTIVITY PARTIAL PURIFICATION PROTEASE BACILLUS CEREUS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Proteases and proteolytic enzymes constitute one of the most important groups of enzymes and are attracting worldwide attentionin attempts to exploit their physiological and biotechnological applications. In this study, partial purifications and biochemicaland antimicrobial characterizations of a protease from Bacillus cereus spp., originally isolated from fermented cabbage, werecarried out. The crude extract obtained after purification, involving ammonium sulphate precipitation and dialysis, was designatedas a partially purified protease (PPP). The obtained PPP had a specific activity of 0.395?2.539 U/g at 32 °C, with maximumactivities for the fractions precipitated at 60 and 80% ammonium sulphate. The PPP activity ranged between 20 and 55 °C, withan optimum temperature at 40 °C. At 60 °C, the PPP retained more than 30% of its activity. The optimum pH for the PPP wasachieved at pH 9, indicating the alkaline source of the enzyme. Protease production was specifically dependent on the calciumconcentration in the culture medium. Also the robustness of the protease on brewer?s spent grain hydrolysis was demonstrated.This suggests a potential eco-friendly application of the enzyme. Finally, it was found that the PPP inhibited the growth ofEscherichia coli O157:H7. This novel property of the PPP liberated by the B. cereus spp. could provide important future benefitsto industry. Fil: Kotlar, Catalina Elena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina Fil: Roura, Sara Ines. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina |
description |
Proteases and proteolytic enzymes constitute one of the most important groups of enzymes and are attracting worldwide attentionin attempts to exploit their physiological and biotechnological applications. In this study, partial purifications and biochemicaland antimicrobial characterizations of a protease from Bacillus cereus spp., originally isolated from fermented cabbage, werecarried out. The crude extract obtained after purification, involving ammonium sulphate precipitation and dialysis, was designatedas a partially purified protease (PPP). The obtained PPP had a specific activity of 0.395?2.539 U/g at 32 °C, with maximumactivities for the fractions precipitated at 60 and 80% ammonium sulphate. The PPP activity ranged between 20 and 55 °C, withan optimum temperature at 40 °C. At 60 °C, the PPP retained more than 30% of its activity. The optimum pH for the PPP wasachieved at pH 9, indicating the alkaline source of the enzyme. Protease production was specifically dependent on the calciumconcentration in the culture medium. Also the robustness of the protease on brewer?s spent grain hydrolysis was demonstrated.This suggests a potential eco-friendly application of the enzyme. Finally, it was found that the PPP inhibited the growth ofEscherichia coli O157:H7. This novel property of the PPP liberated by the B. cereus spp. could provide important future benefitsto industry. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/101700 Kotlar, Catalina Elena; Ponce, Alejandra Graciela; Roura, Sara Ines; Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct; Inst Brewing; Journal of The Institute of Brewing; 121; 7-2015; 558-565 0046-9750 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/101700 |
identifier_str_mv |
Kotlar, Catalina Elena; Ponce, Alejandra Graciela; Roura, Sara Ines; Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct; Inst Brewing; Journal of The Institute of Brewing; 121; 7-2015; 558-565 0046-9750 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jib.257 info:eu-repo/semantics/altIdentifier/doi/10.1002/jib.257 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Inst Brewing |
publisher.none.fl_str_mv |
Inst Brewing |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613500511453184 |
score |
13.070432 |