Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays

Autores
Martinez Marignac, Veronica Lucrecia; Mondragon, Leonel F.; Oertlin, Gloria Susana; Cervantes, Lucia; Cantero, Maria Fernanda; Favant, Jose Luis
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Context: Essiac tea is been used widely in the homeopathy market for cancer treatment. Aims: We hypothesized its use for DNA-damaged mitigation under very low ionizing radiation (IR) on BALB/c mice (10–40 mSv). Settings and Design: The radioprotection of Essiac tea formulae was evidenced by comet assay (CA) and micronucleus (MN) acridine orange staining. We also reported complete blood count, animal weight, and fasting glucose levels to control for tea toxicity. Materials and Methods: Fifty BALB/c male mice of 6-7 week old and pathogen free mice were randomly divided in to control group, control irradiated mice, irradiated and tea or ascorbic acid treated mice, tea treated mice and ascorbic acid treated mice. Genuine Essiac tea treatment was given ad libitum for 7 weeks and ascorbic for no >13 days. The animals were exposed to three different X-ray doses (10 mSv, 20 mSv, and 40 mSv). Statistical Analysis Used: An independent one-tailed t-test or Dunnett's test was used to compare animal weight, fasting glucose levels, white blood count, comet percentage, and MN percentage, between doses, treatment, and controls, after a Welch's ANOVA and Mann–Whitney U-test using Excel worksheets from Biostathandbook.com website. Results: The tea formula resulted in a significant reduction of DNA damaged evidenced by CA (P < 0.01 for dose 3–40 mSv). By MN staining, the peak of significant induction of MNs was by the lower doses, D1 and D2, with a P value = 0.001 and P value = 0.014, respectively; however those irradiated animals when were treated with tea showed reduction of MNs and no significant difference from controls. Conclusions: Using an optimized murine model, we demonstrated that Genuine Essiac tea is not toxic and that it acts as a radioprotector against very low doses of IR.
Fil: Martinez Marignac, Veronica Lucrecia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Mondragon, Leonel F.. Universidad Adventista del Plata; Argentina
Fil: Oertlin, Gloria Susana. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Cervantes, Lucia. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Cantero, Maria Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Favant, Jose Luis. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Materia
CANCER
ESSIAC
XRAY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212344
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212344
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-raysMartinez Marignac, Veronica LucreciaMondragon, Leonel F.Oertlin, Gloria SusanaCervantes, LuciaCantero, Maria FernandaFavant, Jose LuisCANCERESSIACXRAYhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Context: Essiac tea is been used widely in the homeopathy market for cancer treatment. Aims: We hypothesized its use for DNA-damaged mitigation under very low ionizing radiation (IR) on BALB/c mice (10–40 mSv). Settings and Design: The radioprotection of Essiac tea formulae was evidenced by comet assay (CA) and micronucleus (MN) acridine orange staining. We also reported complete blood count, animal weight, and fasting glucose levels to control for tea toxicity. Materials and Methods: Fifty BALB/c male mice of 6-7 week old and pathogen free mice were randomly divided in to control group, control irradiated mice, irradiated and tea or ascorbic acid treated mice, tea treated mice and ascorbic acid treated mice. Genuine Essiac tea treatment was given ad libitum for 7 weeks and ascorbic for no >13 days. The animals were exposed to three different X-ray doses (10 mSv, 20 mSv, and 40 mSv). Statistical Analysis Used: An independent one-tailed t-test or Dunnett's test was used to compare animal weight, fasting glucose levels, white blood count, comet percentage, and MN percentage, between doses, treatment, and controls, after a Welch's ANOVA and Mann–Whitney U-test using Excel worksheets from Biostathandbook.com website. Results: The tea formula resulted in a significant reduction of DNA damaged evidenced by CA (P < 0.01 for dose 3–40 mSv). By MN staining, the peak of significant induction of MNs was by the lower doses, D1 and D2, with a P value = 0.001 and P value = 0.014, respectively; however those irradiated animals when were treated with tea showed reduction of MNs and no significant difference from controls. Conclusions: Using an optimized murine model, we demonstrated that Genuine Essiac tea is not toxic and that it acts as a radioprotector against very low doses of IR.Fil: Martinez Marignac, Veronica Lucrecia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Mondragon, Leonel F.. Universidad Adventista del Plata; ArgentinaFil: Oertlin, Gloria Susana. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Cervantes, Lucia. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; ArgentinaFil: Cantero, Maria Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; ArgentinaFil: Favant, Jose Luis. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; ArgentinaMiddle-Eastern Association for Cancer Research2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212344Martinez Marignac, Veronica Lucrecia; Mondragon, Leonel F.; Oertlin, Gloria Susana; Cervantes, Lucia; Cantero, Maria Fernanda; et al.; Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays; Middle-Eastern Association for Cancer Research; Clinical cancer investigation journal; 9; 4; 8-2020; 126-1352278-0513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ccij-online.org/article/preclinical-study-of-genuine-essiac-formula:-a-cancer-treatment-eight-herbs'-tea-minimizes-dna-insult-of-x-rays-850info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:59Zoai:ri.conicet.gov.ar:11336/212344instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:59.287CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays
title Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays
spellingShingle Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays
Martinez Marignac, Veronica Lucrecia
CANCER
ESSIAC
XRAY
title_short Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays
title_full Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays
title_fullStr Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays
title_full_unstemmed Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays
title_sort Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays
dc.creator.none.fl_str_mv Martinez Marignac, Veronica Lucrecia
Mondragon, Leonel F.
Oertlin, Gloria Susana
Cervantes, Lucia
Cantero, Maria Fernanda
Favant, Jose Luis
author Martinez Marignac, Veronica Lucrecia
author_facet Martinez Marignac, Veronica Lucrecia
Mondragon, Leonel F.
Oertlin, Gloria Susana
Cervantes, Lucia
Cantero, Maria Fernanda
Favant, Jose Luis
author_role author
author2 Mondragon, Leonel F.
Oertlin, Gloria Susana
Cervantes, Lucia
Cantero, Maria Fernanda
Favant, Jose Luis
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CANCER
ESSIAC
XRAY
topic CANCER
ESSIAC
XRAY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Context: Essiac tea is been used widely in the homeopathy market for cancer treatment. Aims: We hypothesized its use for DNA-damaged mitigation under very low ionizing radiation (IR) on BALB/c mice (10–40 mSv). Settings and Design: The radioprotection of Essiac tea formulae was evidenced by comet assay (CA) and micronucleus (MN) acridine orange staining. We also reported complete blood count, animal weight, and fasting glucose levels to control for tea toxicity. Materials and Methods: Fifty BALB/c male mice of 6-7 week old and pathogen free mice were randomly divided in to control group, control irradiated mice, irradiated and tea or ascorbic acid treated mice, tea treated mice and ascorbic acid treated mice. Genuine Essiac tea treatment was given ad libitum for 7 weeks and ascorbic for no >13 days. The animals were exposed to three different X-ray doses (10 mSv, 20 mSv, and 40 mSv). Statistical Analysis Used: An independent one-tailed t-test or Dunnett's test was used to compare animal weight, fasting glucose levels, white blood count, comet percentage, and MN percentage, between doses, treatment, and controls, after a Welch's ANOVA and Mann–Whitney U-test using Excel worksheets from Biostathandbook.com website. Results: The tea formula resulted in a significant reduction of DNA damaged evidenced by CA (P < 0.01 for dose 3–40 mSv). By MN staining, the peak of significant induction of MNs was by the lower doses, D1 and D2, with a P value = 0.001 and P value = 0.014, respectively; however those irradiated animals when were treated with tea showed reduction of MNs and no significant difference from controls. Conclusions: Using an optimized murine model, we demonstrated that Genuine Essiac tea is not toxic and that it acts as a radioprotector against very low doses of IR.
Fil: Martinez Marignac, Veronica Lucrecia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Mondragon, Leonel F.. Universidad Adventista del Plata; Argentina
Fil: Oertlin, Gloria Susana. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Cervantes, Lucia. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Cantero, Maria Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Favant, Jose Luis. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
description Context: Essiac tea is been used widely in the homeopathy market for cancer treatment. Aims: We hypothesized its use for DNA-damaged mitigation under very low ionizing radiation (IR) on BALB/c mice (10–40 mSv). Settings and Design: The radioprotection of Essiac tea formulae was evidenced by comet assay (CA) and micronucleus (MN) acridine orange staining. We also reported complete blood count, animal weight, and fasting glucose levels to control for tea toxicity. Materials and Methods: Fifty BALB/c male mice of 6-7 week old and pathogen free mice were randomly divided in to control group, control irradiated mice, irradiated and tea or ascorbic acid treated mice, tea treated mice and ascorbic acid treated mice. Genuine Essiac tea treatment was given ad libitum for 7 weeks and ascorbic for no >13 days. The animals were exposed to three different X-ray doses (10 mSv, 20 mSv, and 40 mSv). Statistical Analysis Used: An independent one-tailed t-test or Dunnett's test was used to compare animal weight, fasting glucose levels, white blood count, comet percentage, and MN percentage, between doses, treatment, and controls, after a Welch's ANOVA and Mann–Whitney U-test using Excel worksheets from Biostathandbook.com website. Results: The tea formula resulted in a significant reduction of DNA damaged evidenced by CA (P < 0.01 for dose 3–40 mSv). By MN staining, the peak of significant induction of MNs was by the lower doses, D1 and D2, with a P value = 0.001 and P value = 0.014, respectively; however those irradiated animals when were treated with tea showed reduction of MNs and no significant difference from controls. Conclusions: Using an optimized murine model, we demonstrated that Genuine Essiac tea is not toxic and that it acts as a radioprotector against very low doses of IR.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212344
Martinez Marignac, Veronica Lucrecia; Mondragon, Leonel F.; Oertlin, Gloria Susana; Cervantes, Lucia; Cantero, Maria Fernanda; et al.; Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays; Middle-Eastern Association for Cancer Research; Clinical cancer investigation journal; 9; 4; 8-2020; 126-135
2278-0513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212344
identifier_str_mv Martinez Marignac, Veronica Lucrecia; Mondragon, Leonel F.; Oertlin, Gloria Susana; Cervantes, Lucia; Cantero, Maria Fernanda; et al.; Preclinical Study of Genuine Essiac Formula: A Cancer Treatment Eightherbs’ Tea Minimizes DNA Insult of X-rays; Middle-Eastern Association for Cancer Research; Clinical cancer investigation journal; 9; 4; 8-2020; 126-135
2278-0513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ccij-online.org/article/preclinical-study-of-genuine-essiac-formula:-a-cancer-treatment-eight-herbs'-tea-minimizes-dna-insult-of-x-rays-850
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Middle-Eastern Association for Cancer Research
publisher.none.fl_str_mv Middle-Eastern Association for Cancer Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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