Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection

Autores
Ginart, Santiago; Caputo, M.; Corach, Daniel; Sala, Adriana Andrea
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We present the optimization of a previously developed DNA quantification approach that now makes possible the simultaneous detection of Q1a3a Native-American Y-chromosome haplogroup by means of real time-PCR followed by high resolution melting (HRM) analysis. Genotyping is possible by differences in melting temperature (Tm) of one specific Y-chromosome fragment (58bp long, included in the reaction cocktail) which contains the M3-Q3 C/T polymorphism. The specific variant M3-Q3/T (Native-American lineage) has a Tm ≈ 76 °C, whereas M3-Q3/C (non-Native-American) has Tm ≈ 77 °C. Fifty reference male DNA samples (blood and muscle from autopsy) were analyzed. DNA concentration ranged from 0,01 to 28 ng/μL. Thirteen showed Native-American signature (TmT = 76,02 ± 0,04) while thirty-seven as non-Native-American (TmC = 77,13 ± 0,06). These results were compared to those predicted from Y-STRs by White Athey´s on-line software. Casework samples containing male DNA were quantified and successfully haplogrouped. The STRs profiles, subsequently obtained, correlated well with quantitation results. This system represents an approach that provides valuable information at early stages of genotyping process, and useful for choosing the most suitable Y-chromosome database for statistical evaluations.
Fil: Ginart, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina
Fil: Caputo, M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina
Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina
Fil: Sala, Adriana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
DNA QUANTIFICATION
HIGH RESOLUTION MELTING
Q1A3A NATIVE-AMERICAN Y-HAPLOGROUP
SNPS
Y-STR HAPLOTYPE DATABASES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/132546
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/132546
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selectionGinart, SantiagoCaputo, M.Corach, DanielSala, Adriana AndreaDNA QUANTIFICATIONHIGH RESOLUTION MELTINGQ1A3A NATIVE-AMERICAN Y-HAPLOGROUPSNPSY-STR HAPLOTYPE DATABASEShttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3We present the optimization of a previously developed DNA quantification approach that now makes possible the simultaneous detection of Q1a3a Native-American Y-chromosome haplogroup by means of real time-PCR followed by high resolution melting (HRM) analysis. Genotyping is possible by differences in melting temperature (Tm) of one specific Y-chromosome fragment (58bp long, included in the reaction cocktail) which contains the M3-Q3 C/T polymorphism. The specific variant M3-Q3/T (Native-American lineage) has a Tm ≈ 76 °C, whereas M3-Q3/C (non-Native-American) has Tm ≈ 77 °C. Fifty reference male DNA samples (blood and muscle from autopsy) were analyzed. DNA concentration ranged from 0,01 to 28 ng/μL. Thirteen showed Native-American signature (TmT = 76,02 ± 0,04) while thirty-seven as non-Native-American (TmC = 77,13 ± 0,06). These results were compared to those predicted from Y-STRs by White Athey´s on-line software. Casework samples containing male DNA were quantified and successfully haplogrouped. The STRs profiles, subsequently obtained, correlated well with quantitation results. This system represents an approach that provides valuable information at early stages of genotyping process, and useful for choosing the most suitable Y-chromosome database for statistical evaluations.Fil: Ginart, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; ArgentinaFil: Caputo, M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; ArgentinaFil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; ArgentinaFil: Sala, Adriana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132546Ginart, Santiago; Caputo, M.; Corach, Daniel; Sala, Adriana Andrea; Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection; Elsevier; Forensic Science International: Genetics Supplement Series; 7; 1; 12-2019; 315-3171875-17681875-175XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1875176819304494info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsigss.2019.09.119info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:50Zoai:ri.conicet.gov.ar:11336/132546instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:50.369CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection
title Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection
spellingShingle Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection
Ginart, Santiago
DNA QUANTIFICATION
HIGH RESOLUTION MELTING
Q1A3A NATIVE-AMERICAN Y-HAPLOGROUP
SNPS
Y-STR HAPLOTYPE DATABASES
title_short Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection
title_full Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection
title_fullStr Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection
title_full_unstemmed Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection
title_sort Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection
dc.creator.none.fl_str_mv Ginart, Santiago
Caputo, M.
Corach, Daniel
Sala, Adriana Andrea
author Ginart, Santiago
author_facet Ginart, Santiago
Caputo, M.
Corach, Daniel
Sala, Adriana Andrea
author_role author
author2 Caputo, M.
Corach, Daniel
Sala, Adriana Andrea
author2_role author
author
author
dc.subject.none.fl_str_mv DNA QUANTIFICATION
HIGH RESOLUTION MELTING
Q1A3A NATIVE-AMERICAN Y-HAPLOGROUP
SNPS
Y-STR HAPLOTYPE DATABASES
topic DNA QUANTIFICATION
HIGH RESOLUTION MELTING
Q1A3A NATIVE-AMERICAN Y-HAPLOGROUP
SNPS
Y-STR HAPLOTYPE DATABASES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We present the optimization of a previously developed DNA quantification approach that now makes possible the simultaneous detection of Q1a3a Native-American Y-chromosome haplogroup by means of real time-PCR followed by high resolution melting (HRM) analysis. Genotyping is possible by differences in melting temperature (Tm) of one specific Y-chromosome fragment (58bp long, included in the reaction cocktail) which contains the M3-Q3 C/T polymorphism. The specific variant M3-Q3/T (Native-American lineage) has a Tm ≈ 76 °C, whereas M3-Q3/C (non-Native-American) has Tm ≈ 77 °C. Fifty reference male DNA samples (blood and muscle from autopsy) were analyzed. DNA concentration ranged from 0,01 to 28 ng/μL. Thirteen showed Native-American signature (TmT = 76,02 ± 0,04) while thirty-seven as non-Native-American (TmC = 77,13 ± 0,06). These results were compared to those predicted from Y-STRs by White Athey´s on-line software. Casework samples containing male DNA were quantified and successfully haplogrouped. The STRs profiles, subsequently obtained, correlated well with quantitation results. This system represents an approach that provides valuable information at early stages of genotyping process, and useful for choosing the most suitable Y-chromosome database for statistical evaluations.
Fil: Ginart, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina
Fil: Caputo, M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina
Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina
Fil: Sala, Adriana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description We present the optimization of a previously developed DNA quantification approach that now makes possible the simultaneous detection of Q1a3a Native-American Y-chromosome haplogroup by means of real time-PCR followed by high resolution melting (HRM) analysis. Genotyping is possible by differences in melting temperature (Tm) of one specific Y-chromosome fragment (58bp long, included in the reaction cocktail) which contains the M3-Q3 C/T polymorphism. The specific variant M3-Q3/T (Native-American lineage) has a Tm ≈ 76 °C, whereas M3-Q3/C (non-Native-American) has Tm ≈ 77 °C. Fifty reference male DNA samples (blood and muscle from autopsy) were analyzed. DNA concentration ranged from 0,01 to 28 ng/μL. Thirteen showed Native-American signature (TmT = 76,02 ± 0,04) while thirty-seven as non-Native-American (TmC = 77,13 ± 0,06). These results were compared to those predicted from Y-STRs by White Athey´s on-line software. Casework samples containing male DNA were quantified and successfully haplogrouped. The STRs profiles, subsequently obtained, correlated well with quantitation results. This system represents an approach that provides valuable information at early stages of genotyping process, and useful for choosing the most suitable Y-chromosome database for statistical evaluations.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/132546
Ginart, Santiago; Caputo, M.; Corach, Daniel; Sala, Adriana Andrea; Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection; Elsevier; Forensic Science International: Genetics Supplement Series; 7; 1; 12-2019; 315-317
1875-1768
1875-175X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/132546
identifier_str_mv Ginart, Santiago; Caputo, M.; Corach, Daniel; Sala, Adriana Andrea; Q1a3a native-American Y-haplogroup detection in DNA quantification step: A quick diagnosis for Y-chromosome database selection; Elsevier; Forensic Science International: Genetics Supplement Series; 7; 1; 12-2019; 315-317
1875-1768
1875-175X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1875176819304494
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsigss.2019.09.119
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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