α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility

Autores
Arcos, Alexis; de Paola, Maria Matilde; Gianetti, Diego; Acuña, Diego; Velásquez, Zahady D.; Miró, María Paz; Toro, Gabriela; Hinrichsen, Bryan; Muñoz, Rosa Iris; Lin, Yimo; Mardones, Gonzalo A.; Ehrenfeld, Pamela; Rivera, Francisco J.; Michaut, Marcela Alejandra; Batiz, Luis Federico
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role of multifunctional proteins able to simultaneously coordinate/modulate several cellular pathways is unclear. Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (α-SNAP) is a multifunctional protein that participates in SNARE-mediated membrane fusion events. In addition, it regulates cell-to-cell adhesion, AMPK signaling, autophagy and apoptosis in different cell types. In this study we examined the expression pattern of α-SNAP in ovarian tissue and the consequences of α-SNAP (M105I) mutation (hyh mutation) in folliculogenesis and female fertility. Our results showed that α-SNAP protein is highly expressed in GCs and its expression is modulated by gonadotropin stimuli. On the other hand, α-SNAP-mutant mice show a reduction in α-SNAP protein levels. Moreover, increased apoptosis of GCs and follicular atresia, reduced ovulation rate, and a dramatic decline in fertility is observed in α-SNAP-mutant females. In conclusion, α-SNAP plays a critical role in the balance between follicular development and atresia. Consequently, a reduction in its expression/function (M105I mutation) causes early depletion of ovarian follicles and female subfertility.
Fil: Arcos, Alexis. Universidad Austral de Chile; Chile
Fil: de Paola, Maria Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Gianetti, Diego. Universidad Austral de Chile; Chile
Fil: Acuña, Diego. Universidad Austral de Chile; Chile
Fil: Velásquez, Zahady D.. Universidad Austral de Chile; Chile
Fil: Miró, María Paz. Universidad Austral de Chile; Chile
Fil: Toro, Gabriela. Universidad Austral de Chile; Chile
Fil: Hinrichsen, Bryan. Universidad Austral de Chile; Chile
Fil: Muñoz, Rosa Iris. Universidad Austral de Chile; Chile
Fil: Lin, Yimo. Oregon Health and Science University; Estados Unidos
Fil: Mardones, Gonzalo A.. Universidad Austral de Chile; Chile
Fil: Ehrenfeld, Pamela. Universidad Austral de Chile; Chile
Fil: Rivera, Francisco J.. Paracelsus Medizinische Privatuniversitat; Austria. Universidad Austral de Chile; Chile
Fil: Michaut, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Batiz, Luis Federico. Universidad Austral de Chile; Chile. Universidad de los Andes; Venezuela
Materia
α-SNAP
ovarian granulosa cells
folliculogenesis
female fertility
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49678
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/49678
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertilityArcos, Alexisde Paola, Maria MatildeGianetti, DiegoAcuña, DiegoVelásquez, Zahady D.Miró, María PazToro, GabrielaHinrichsen, BryanMuñoz, Rosa IrisLin, YimoMardones, Gonzalo A.Ehrenfeld, PamelaRivera, Francisco J.Michaut, Marcela AlejandraBatiz, Luis Federicoα-SNAPovarian granulosa cellsfolliculogenesisfemale fertilityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role of multifunctional proteins able to simultaneously coordinate/modulate several cellular pathways is unclear. Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (α-SNAP) is a multifunctional protein that participates in SNARE-mediated membrane fusion events. In addition, it regulates cell-to-cell adhesion, AMPK signaling, autophagy and apoptosis in different cell types. In this study we examined the expression pattern of α-SNAP in ovarian tissue and the consequences of α-SNAP (M105I) mutation (hyh mutation) in folliculogenesis and female fertility. Our results showed that α-SNAP protein is highly expressed in GCs and its expression is modulated by gonadotropin stimuli. On the other hand, α-SNAP-mutant mice show a reduction in α-SNAP protein levels. Moreover, increased apoptosis of GCs and follicular atresia, reduced ovulation rate, and a dramatic decline in fertility is observed in α-SNAP-mutant females. In conclusion, α-SNAP plays a critical role in the balance between follicular development and atresia. Consequently, a reduction in its expression/function (M105I mutation) causes early depletion of ovarian follicles and female subfertility.Fil: Arcos, Alexis. Universidad Austral de Chile; ChileFil: de Paola, Maria Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Gianetti, Diego. Universidad Austral de Chile; ChileFil: Acuña, Diego. Universidad Austral de Chile; ChileFil: Velásquez, Zahady D.. Universidad Austral de Chile; ChileFil: Miró, María Paz. Universidad Austral de Chile; ChileFil: Toro, Gabriela. Universidad Austral de Chile; ChileFil: Hinrichsen, Bryan. Universidad Austral de Chile; ChileFil: Muñoz, Rosa Iris. Universidad Austral de Chile; ChileFil: Lin, Yimo. Oregon Health and Science University; Estados UnidosFil: Mardones, Gonzalo A.. Universidad Austral de Chile; ChileFil: Ehrenfeld, Pamela. Universidad Austral de Chile; ChileFil: Rivera, Francisco J.. Paracelsus Medizinische Privatuniversitat; Austria. Universidad Austral de Chile; ChileFil: Michaut, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Batiz, Luis Federico. Universidad Austral de Chile; Chile. Universidad de los Andes; VenezuelaNature Publishing Group2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49678Arcos, Alexis; de Paola, Maria Matilde; Gianetti, Diego; Acuña, Diego; Velásquez, Zahady D.; et al.; α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-142045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-12292-9info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-12292-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:53Zoai:ri.conicet.gov.ar:11336/49678instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:53.394CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
spellingShingle α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
Arcos, Alexis
α-SNAP
ovarian granulosa cells
folliculogenesis
female fertility
title_short α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title_full α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title_fullStr α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title_full_unstemmed α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title_sort α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
dc.creator.none.fl_str_mv Arcos, Alexis
de Paola, Maria Matilde
Gianetti, Diego
Acuña, Diego
Velásquez, Zahady D.
Miró, María Paz
Toro, Gabriela
Hinrichsen, Bryan
Muñoz, Rosa Iris
Lin, Yimo
Mardones, Gonzalo A.
Ehrenfeld, Pamela
Rivera, Francisco J.
Michaut, Marcela Alejandra
Batiz, Luis Federico
author Arcos, Alexis
author_facet Arcos, Alexis
de Paola, Maria Matilde
Gianetti, Diego
Acuña, Diego
Velásquez, Zahady D.
Miró, María Paz
Toro, Gabriela
Hinrichsen, Bryan
Muñoz, Rosa Iris
Lin, Yimo
Mardones, Gonzalo A.
Ehrenfeld, Pamela
Rivera, Francisco J.
Michaut, Marcela Alejandra
Batiz, Luis Federico
author_role author
author2 de Paola, Maria Matilde
Gianetti, Diego
Acuña, Diego
Velásquez, Zahady D.
Miró, María Paz
Toro, Gabriela
Hinrichsen, Bryan
Muñoz, Rosa Iris
Lin, Yimo
Mardones, Gonzalo A.
Ehrenfeld, Pamela
Rivera, Francisco J.
Michaut, Marcela Alejandra
Batiz, Luis Federico
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv α-SNAP
ovarian granulosa cells
folliculogenesis
female fertility
topic α-SNAP
ovarian granulosa cells
folliculogenesis
female fertility
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role of multifunctional proteins able to simultaneously coordinate/modulate several cellular pathways is unclear. Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (α-SNAP) is a multifunctional protein that participates in SNARE-mediated membrane fusion events. In addition, it regulates cell-to-cell adhesion, AMPK signaling, autophagy and apoptosis in different cell types. In this study we examined the expression pattern of α-SNAP in ovarian tissue and the consequences of α-SNAP (M105I) mutation (hyh mutation) in folliculogenesis and female fertility. Our results showed that α-SNAP protein is highly expressed in GCs and its expression is modulated by gonadotropin stimuli. On the other hand, α-SNAP-mutant mice show a reduction in α-SNAP protein levels. Moreover, increased apoptosis of GCs and follicular atresia, reduced ovulation rate, and a dramatic decline in fertility is observed in α-SNAP-mutant females. In conclusion, α-SNAP plays a critical role in the balance between follicular development and atresia. Consequently, a reduction in its expression/function (M105I mutation) causes early depletion of ovarian follicles and female subfertility.
Fil: Arcos, Alexis. Universidad Austral de Chile; Chile
Fil: de Paola, Maria Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Gianetti, Diego. Universidad Austral de Chile; Chile
Fil: Acuña, Diego. Universidad Austral de Chile; Chile
Fil: Velásquez, Zahady D.. Universidad Austral de Chile; Chile
Fil: Miró, María Paz. Universidad Austral de Chile; Chile
Fil: Toro, Gabriela. Universidad Austral de Chile; Chile
Fil: Hinrichsen, Bryan. Universidad Austral de Chile; Chile
Fil: Muñoz, Rosa Iris. Universidad Austral de Chile; Chile
Fil: Lin, Yimo. Oregon Health and Science University; Estados Unidos
Fil: Mardones, Gonzalo A.. Universidad Austral de Chile; Chile
Fil: Ehrenfeld, Pamela. Universidad Austral de Chile; Chile
Fil: Rivera, Francisco J.. Paracelsus Medizinische Privatuniversitat; Austria. Universidad Austral de Chile; Chile
Fil: Michaut, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Batiz, Luis Federico. Universidad Austral de Chile; Chile. Universidad de los Andes; Venezuela
description The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role of multifunctional proteins able to simultaneously coordinate/modulate several cellular pathways is unclear. Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (α-SNAP) is a multifunctional protein that participates in SNARE-mediated membrane fusion events. In addition, it regulates cell-to-cell adhesion, AMPK signaling, autophagy and apoptosis in different cell types. In this study we examined the expression pattern of α-SNAP in ovarian tissue and the consequences of α-SNAP (M105I) mutation (hyh mutation) in folliculogenesis and female fertility. Our results showed that α-SNAP protein is highly expressed in GCs and its expression is modulated by gonadotropin stimuli. On the other hand, α-SNAP-mutant mice show a reduction in α-SNAP protein levels. Moreover, increased apoptosis of GCs and follicular atresia, reduced ovulation rate, and a dramatic decline in fertility is observed in α-SNAP-mutant females. In conclusion, α-SNAP plays a critical role in the balance between follicular development and atresia. Consequently, a reduction in its expression/function (M105I mutation) causes early depletion of ovarian follicles and female subfertility.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49678
Arcos, Alexis; de Paola, Maria Matilde; Gianetti, Diego; Acuña, Diego; Velásquez, Zahady D.; et al.; α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-14
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49678
identifier_str_mv Arcos, Alexis; de Paola, Maria Matilde; Gianetti, Diego; Acuña, Diego; Velásquez, Zahady D.; et al.; α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-14
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-12292-9
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-12292-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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