Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells

Autores
Rodriguez, Ernesto; Noya, Verónica; Cervi, Laura Alejandra; Chiribao, Maria Laura; Brossard, Natalie; Chiale, Carolina; Carmona, Carlos; Giacomini, Cecilia; Freire, Teresa
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Helminths express various carbohydrate-containing glycoconjugates on their surface, and they release glycan-rich excretion/secretion products that can be very important in their life cycles, infection and pathology. Recent evidence suggests that parasite glycoconjugates could play a role in the evasion of the immune response, leading to a modified Th2-polarized immune response that favors parasite survival in the host. Nevertheless, there is limited information about the nature or function of glycans produced by the trematode Fasciola hepatica, the causative agent of fasciolosis. In this paper, we investigate whether glycosylated molecules from F. hepatica participate in the modulation of host immunity. We also focus on dendritic cells, since they are an important target of immune-modulation by helminths, affecting their activity or function. Our results indicate that glycans from F. hepatica promote the production of IL-4 and IL-10, suppressing IFNγ production. During infection, this parasite is able to induce a semi-mature phenotype of DCs expressing low levels of MHCII and secrete IL-10. Furthermore, we show that parasite glycoconjugates mediate the modulation of LPS-induced maturation of DCs since their oxidation restores the capacity of LPS-treated DCs to secrete high levels of the pro-inflammatory cytokines IL-6 and IL-12/23p40 and low levels of the anti-inflammatory cytokine IL-10. Inhibition assays using carbohydrates suggest that the immune-modulation is mediated, at least in part, by the recognition of a mannose specific-CLR that signals by recruiting the phosphatase Php2. The results presented here contribute to the understanding of the role of parasite glycosylated molecules in the modulation of the host immunity and might be useful in the design of vaccines against fasciolosis.
Fil: Rodriguez, Ernesto. Universidad de la República; Uruguay
Fil: Noya, Verónica. Universidad de la República; Uruguay
Fil: Cervi, Laura Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Chiribao, Maria Laura. Universidad de la República; Uruguay
Fil: Brossard, Natalie. Universidad de la República; Uruguay
Fil: Chiale, Carolina. Universidad de la República; Uruguay
Fil: Carmona, Carlos. Universidad de la República; Uruguay
Fil: Giacomini, Cecilia. Universidad de la República; Uruguay
Fil: Freire, Teresa. Universidad de la República; Uruguay
Materia
FASCIOLA HEPATICA
GLYCANS
DENDRITIC CELLS
TLR: MATURATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46078
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46078
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cellsRodriguez, ErnestoNoya, VerónicaCervi, Laura AlejandraChiribao, Maria LauraBrossard, NatalieChiale, CarolinaCarmona, CarlosGiacomini, CeciliaFreire, TeresaFASCIOLA HEPATICAGLYCANSDENDRITIC CELLSTLR: MATURATIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Helminths express various carbohydrate-containing glycoconjugates on their surface, and they release glycan-rich excretion/secretion products that can be very important in their life cycles, infection and pathology. Recent evidence suggests that parasite glycoconjugates could play a role in the evasion of the immune response, leading to a modified Th2-polarized immune response that favors parasite survival in the host. Nevertheless, there is limited information about the nature or function of glycans produced by the trematode Fasciola hepatica, the causative agent of fasciolosis. In this paper, we investigate whether glycosylated molecules from F. hepatica participate in the modulation of host immunity. We also focus on dendritic cells, since they are an important target of immune-modulation by helminths, affecting their activity or function. Our results indicate that glycans from F. hepatica promote the production of IL-4 and IL-10, suppressing IFNγ production. During infection, this parasite is able to induce a semi-mature phenotype of DCs expressing low levels of MHCII and secrete IL-10. Furthermore, we show that parasite glycoconjugates mediate the modulation of LPS-induced maturation of DCs since their oxidation restores the capacity of LPS-treated DCs to secrete high levels of the pro-inflammatory cytokines IL-6 and IL-12/23p40 and low levels of the anti-inflammatory cytokine IL-10. Inhibition assays using carbohydrates suggest that the immune-modulation is mediated, at least in part, by the recognition of a mannose specific-CLR that signals by recruiting the phosphatase Php2. The results presented here contribute to the understanding of the role of parasite glycosylated molecules in the modulation of the host immunity and might be useful in the design of vaccines against fasciolosis.Fil: Rodriguez, Ernesto. Universidad de la República; UruguayFil: Noya, Verónica. Universidad de la República; UruguayFil: Cervi, Laura Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Chiribao, Maria Laura. Universidad de la República; UruguayFil: Brossard, Natalie. Universidad de la República; UruguayFil: Chiale, Carolina. Universidad de la República; UruguayFil: Carmona, Carlos. Universidad de la República; UruguayFil: Giacomini, Cecilia. Universidad de la República; UruguayFil: Freire, Teresa. Universidad de la República; UruguayPublic Library of Science2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46078Rodriguez, Ernesto; Noya, Verónica; Cervi, Laura Alejandra; Chiribao, Maria Laura; Brossard, Natalie; et al.; Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells; Public Library of Science; Neglected Tropical Diseases; 31; 12; 12-2015; 1-261935-27271935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0004234info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004234info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:13Zoai:ri.conicet.gov.ar:11336/46078instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:13.849CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells
title Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells
spellingShingle Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells
Rodriguez, Ernesto
FASCIOLA HEPATICA
GLYCANS
DENDRITIC CELLS
TLR: MATURATION
title_short Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells
title_full Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells
title_fullStr Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells
title_full_unstemmed Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells
title_sort Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells
dc.creator.none.fl_str_mv Rodriguez, Ernesto
Noya, Verónica
Cervi, Laura Alejandra
Chiribao, Maria Laura
Brossard, Natalie
Chiale, Carolina
Carmona, Carlos
Giacomini, Cecilia
Freire, Teresa
author Rodriguez, Ernesto
author_facet Rodriguez, Ernesto
Noya, Verónica
Cervi, Laura Alejandra
Chiribao, Maria Laura
Brossard, Natalie
Chiale, Carolina
Carmona, Carlos
Giacomini, Cecilia
Freire, Teresa
author_role author
author2 Noya, Verónica
Cervi, Laura Alejandra
Chiribao, Maria Laura
Brossard, Natalie
Chiale, Carolina
Carmona, Carlos
Giacomini, Cecilia
Freire, Teresa
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FASCIOLA HEPATICA
GLYCANS
DENDRITIC CELLS
TLR: MATURATION
topic FASCIOLA HEPATICA
GLYCANS
DENDRITIC CELLS
TLR: MATURATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Helminths express various carbohydrate-containing glycoconjugates on their surface, and they release glycan-rich excretion/secretion products that can be very important in their life cycles, infection and pathology. Recent evidence suggests that parasite glycoconjugates could play a role in the evasion of the immune response, leading to a modified Th2-polarized immune response that favors parasite survival in the host. Nevertheless, there is limited information about the nature or function of glycans produced by the trematode Fasciola hepatica, the causative agent of fasciolosis. In this paper, we investigate whether glycosylated molecules from F. hepatica participate in the modulation of host immunity. We also focus on dendritic cells, since they are an important target of immune-modulation by helminths, affecting their activity or function. Our results indicate that glycans from F. hepatica promote the production of IL-4 and IL-10, suppressing IFNγ production. During infection, this parasite is able to induce a semi-mature phenotype of DCs expressing low levels of MHCII and secrete IL-10. Furthermore, we show that parasite glycoconjugates mediate the modulation of LPS-induced maturation of DCs since their oxidation restores the capacity of LPS-treated DCs to secrete high levels of the pro-inflammatory cytokines IL-6 and IL-12/23p40 and low levels of the anti-inflammatory cytokine IL-10. Inhibition assays using carbohydrates suggest that the immune-modulation is mediated, at least in part, by the recognition of a mannose specific-CLR that signals by recruiting the phosphatase Php2. The results presented here contribute to the understanding of the role of parasite glycosylated molecules in the modulation of the host immunity and might be useful in the design of vaccines against fasciolosis.
Fil: Rodriguez, Ernesto. Universidad de la República; Uruguay
Fil: Noya, Verónica. Universidad de la República; Uruguay
Fil: Cervi, Laura Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Chiribao, Maria Laura. Universidad de la República; Uruguay
Fil: Brossard, Natalie. Universidad de la República; Uruguay
Fil: Chiale, Carolina. Universidad de la República; Uruguay
Fil: Carmona, Carlos. Universidad de la República; Uruguay
Fil: Giacomini, Cecilia. Universidad de la República; Uruguay
Fil: Freire, Teresa. Universidad de la República; Uruguay
description Helminths express various carbohydrate-containing glycoconjugates on their surface, and they release glycan-rich excretion/secretion products that can be very important in their life cycles, infection and pathology. Recent evidence suggests that parasite glycoconjugates could play a role in the evasion of the immune response, leading to a modified Th2-polarized immune response that favors parasite survival in the host. Nevertheless, there is limited information about the nature or function of glycans produced by the trematode Fasciola hepatica, the causative agent of fasciolosis. In this paper, we investigate whether glycosylated molecules from F. hepatica participate in the modulation of host immunity. We also focus on dendritic cells, since they are an important target of immune-modulation by helminths, affecting their activity or function. Our results indicate that glycans from F. hepatica promote the production of IL-4 and IL-10, suppressing IFNγ production. During infection, this parasite is able to induce a semi-mature phenotype of DCs expressing low levels of MHCII and secrete IL-10. Furthermore, we show that parasite glycoconjugates mediate the modulation of LPS-induced maturation of DCs since their oxidation restores the capacity of LPS-treated DCs to secrete high levels of the pro-inflammatory cytokines IL-6 and IL-12/23p40 and low levels of the anti-inflammatory cytokine IL-10. Inhibition assays using carbohydrates suggest that the immune-modulation is mediated, at least in part, by the recognition of a mannose specific-CLR that signals by recruiting the phosphatase Php2. The results presented here contribute to the understanding of the role of parasite glycosylated molecules in the modulation of the host immunity and might be useful in the design of vaccines against fasciolosis.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46078
Rodriguez, Ernesto; Noya, Verónica; Cervi, Laura Alejandra; Chiribao, Maria Laura; Brossard, Natalie; et al.; Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells; Public Library of Science; Neglected Tropical Diseases; 31; 12; 12-2015; 1-26
1935-2727
1935-2735
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46078
identifier_str_mv Rodriguez, Ernesto; Noya, Verónica; Cervi, Laura Alejandra; Chiribao, Maria Laura; Brossard, Natalie; et al.; Glycans from Fasciola hepatica modulate the host immune response and TLR-Induced maturation of dendritic cells; Public Library of Science; Neglected Tropical Diseases; 31; 12; 12-2015; 1-26
1935-2727
1935-2735
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0004234
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004234
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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