Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development

Autores
Noya, Veronica; Rodriguez, Ernesto; Cervi, Laura Alejandra; Giacomini, Cecilia; Brossard, Natalie; Chiale, Carolina; Carmona, Carlos; Freire, Teresa
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fasciola hepatica is a worldwide distributed helminth pathogen that causes great economic losses in sheep andcattle. This parasite is able to regulate the host immune response, producing high levels of IL-5 and low levels ofIFNγ, as well as modulating the function of dendritic cells (DCs), mast cells or macrophages, among others.Moreover, TLR-mediated maturation of DCs can be suppressed by F. hepatica derived components. Here, weinvestigated the role of glycans in the modulation of LPS-induced maturation of DCs, as well as in the production ofIL-5 and IFNγ by splenocytes from infected mice. We show that F. hepatica induces the recruitment to theperitoneum of semi-matured DCs, as judged by a down-regulation of MHC class II molecule expression and anincrease of CD80 and CD86 expression of DCs in the peritoneum of infected animals. Furthermore, we provideevidence indicating that glycan structures from F. hepatica are responsible, at least in part, for inhibiting LPS-induced DC maturation and production of IFNγ by splenocytes from infected animals. On the other hand, we showthat a mucin-like non-glycosylated peptide highly expressed in NEJ (Fhmuc) is able to synergize with LPS ininducing DC-maturation, and that it induces a T cell response specific for F. hepatica, both alone or in combinationwith DCs. Our data highlight the role of F. hepatica glycans in modulating the host immune response and mightcontribute to the design of vaccines against fasciolosis.
Fil: Noya, Veronica. Universidad de la República; Uruguay
Fil: Rodriguez, Ernesto. Universidad de la República; Uruguay
Fil: Cervi, Laura Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Giacomini, Cecilia. Universidad de la República; Uruguay
Fil: Brossard, Natalie. Universidad de la República; Uruguay
Fil: Chiale, Carolina. Universidad de la República; Uruguay
Fil: Carmona, Carlos. Universidad de la República; Uruguay
Fil: Freire, Teresa. Universidad de la República; Uruguay
Materia
Fasciola hepatica
Glycan
Mucin
Dendritic cell
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31881
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31881
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine DevelopmentNoya, VeronicaRodriguez, ErnestoCervi, Laura AlejandraGiacomini, CeciliaBrossard, NatalieChiale, CarolinaCarmona, CarlosFreire, TeresaFasciola hepaticaGlycanMucinDendritic cellhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Fasciola hepatica is a worldwide distributed helminth pathogen that causes great economic losses in sheep andcattle. This parasite is able to regulate the host immune response, producing high levels of IL-5 and low levels ofIFNγ, as well as modulating the function of dendritic cells (DCs), mast cells or macrophages, among others.Moreover, TLR-mediated maturation of DCs can be suppressed by F. hepatica derived components. Here, weinvestigated the role of glycans in the modulation of LPS-induced maturation of DCs, as well as in the production ofIL-5 and IFNγ by splenocytes from infected mice. We show that F. hepatica induces the recruitment to theperitoneum of semi-matured DCs, as judged by a down-regulation of MHC class II molecule expression and anincrease of CD80 and CD86 expression of DCs in the peritoneum of infected animals. Furthermore, we provideevidence indicating that glycan structures from F. hepatica are responsible, at least in part, for inhibiting LPS-induced DC maturation and production of IFNγ by splenocytes from infected animals. On the other hand, we showthat a mucin-like non-glycosylated peptide highly expressed in NEJ (Fhmuc) is able to synergize with LPS ininducing DC-maturation, and that it induces a T cell response specific for F. hepatica, both alone or in combinationwith DCs. Our data highlight the role of F. hepatica glycans in modulating the host immune response and mightcontribute to the design of vaccines against fasciolosis.Fil: Noya, Veronica. Universidad de la República; UruguayFil: Rodriguez, Ernesto. Universidad de la República; UruguayFil: Cervi, Laura Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Giacomini, Cecilia. Universidad de la República; UruguayFil: Brossard, Natalie. Universidad de la República; UruguayFil: Chiale, Carolina. Universidad de la República; UruguayFil: Carmona, Carlos. Universidad de la República; UruguayFil: Freire, Teresa. Universidad de la República; UruguayOMICS Publlishing group2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31881Freire, Teresa; Carmona, Carlos; Chiale, Carolina; Brossard, Natalie; Giacomini, Cecilia; Cervi, Laura Alejandra; et al.; Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development; OMICS Publlishing group; Journal of Vaccines and Vaccination; 5; 4; 6-2014; 1-82157-7560CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7560.1000233info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/modulation-of-dendritic-cell-maturation-by-fasciola-hepatica-implications-of-glycans-and-mucins-for-vaccine-development-2157-7560-5-233.php?aid=28636info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:52Zoai:ri.conicet.gov.ar:11336/31881instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:52.334CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development
title Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development
spellingShingle Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development
Noya, Veronica
Fasciola hepatica
Glycan
Mucin
Dendritic cell
title_short Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development
title_full Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development
title_fullStr Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development
title_full_unstemmed Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development
title_sort Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development
dc.creator.none.fl_str_mv Noya, Veronica
Rodriguez, Ernesto
Cervi, Laura Alejandra
Giacomini, Cecilia
Brossard, Natalie
Chiale, Carolina
Carmona, Carlos
Freire, Teresa
author Noya, Veronica
author_facet Noya, Veronica
Rodriguez, Ernesto
Cervi, Laura Alejandra
Giacomini, Cecilia
Brossard, Natalie
Chiale, Carolina
Carmona, Carlos
Freire, Teresa
author_role author
author2 Rodriguez, Ernesto
Cervi, Laura Alejandra
Giacomini, Cecilia
Brossard, Natalie
Chiale, Carolina
Carmona, Carlos
Freire, Teresa
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fasciola hepatica
Glycan
Mucin
Dendritic cell
topic Fasciola hepatica
Glycan
Mucin
Dendritic cell
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Fasciola hepatica is a worldwide distributed helminth pathogen that causes great economic losses in sheep andcattle. This parasite is able to regulate the host immune response, producing high levels of IL-5 and low levels ofIFNγ, as well as modulating the function of dendritic cells (DCs), mast cells or macrophages, among others.Moreover, TLR-mediated maturation of DCs can be suppressed by F. hepatica derived components. Here, weinvestigated the role of glycans in the modulation of LPS-induced maturation of DCs, as well as in the production ofIL-5 and IFNγ by splenocytes from infected mice. We show that F. hepatica induces the recruitment to theperitoneum of semi-matured DCs, as judged by a down-regulation of MHC class II molecule expression and anincrease of CD80 and CD86 expression of DCs in the peritoneum of infected animals. Furthermore, we provideevidence indicating that glycan structures from F. hepatica are responsible, at least in part, for inhibiting LPS-induced DC maturation and production of IFNγ by splenocytes from infected animals. On the other hand, we showthat a mucin-like non-glycosylated peptide highly expressed in NEJ (Fhmuc) is able to synergize with LPS ininducing DC-maturation, and that it induces a T cell response specific for F. hepatica, both alone or in combinationwith DCs. Our data highlight the role of F. hepatica glycans in modulating the host immune response and mightcontribute to the design of vaccines against fasciolosis.
Fil: Noya, Veronica. Universidad de la República; Uruguay
Fil: Rodriguez, Ernesto. Universidad de la República; Uruguay
Fil: Cervi, Laura Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Giacomini, Cecilia. Universidad de la República; Uruguay
Fil: Brossard, Natalie. Universidad de la República; Uruguay
Fil: Chiale, Carolina. Universidad de la República; Uruguay
Fil: Carmona, Carlos. Universidad de la República; Uruguay
Fil: Freire, Teresa. Universidad de la República; Uruguay
description Fasciola hepatica is a worldwide distributed helminth pathogen that causes great economic losses in sheep andcattle. This parasite is able to regulate the host immune response, producing high levels of IL-5 and low levels ofIFNγ, as well as modulating the function of dendritic cells (DCs), mast cells or macrophages, among others.Moreover, TLR-mediated maturation of DCs can be suppressed by F. hepatica derived components. Here, weinvestigated the role of glycans in the modulation of LPS-induced maturation of DCs, as well as in the production ofIL-5 and IFNγ by splenocytes from infected mice. We show that F. hepatica induces the recruitment to theperitoneum of semi-matured DCs, as judged by a down-regulation of MHC class II molecule expression and anincrease of CD80 and CD86 expression of DCs in the peritoneum of infected animals. Furthermore, we provideevidence indicating that glycan structures from F. hepatica are responsible, at least in part, for inhibiting LPS-induced DC maturation and production of IFNγ by splenocytes from infected animals. On the other hand, we showthat a mucin-like non-glycosylated peptide highly expressed in NEJ (Fhmuc) is able to synergize with LPS ininducing DC-maturation, and that it induces a T cell response specific for F. hepatica, both alone or in combinationwith DCs. Our data highlight the role of F. hepatica glycans in modulating the host immune response and mightcontribute to the design of vaccines against fasciolosis.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31881
Freire, Teresa; Carmona, Carlos; Chiale, Carolina; Brossard, Natalie; Giacomini, Cecilia; Cervi, Laura Alejandra; et al.; Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development; OMICS Publlishing group; Journal of Vaccines and Vaccination; 5; 4; 6-2014; 1-8
2157-7560
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31881
identifier_str_mv Freire, Teresa; Carmona, Carlos; Chiale, Carolina; Brossard, Natalie; Giacomini, Cecilia; Cervi, Laura Alejandra; et al.; Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of Glycans and Mucins for Vaccine Development; OMICS Publlishing group; Journal of Vaccines and Vaccination; 5; 4; 6-2014; 1-8
2157-7560
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7560.1000233
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/modulation-of-dendritic-cell-maturation-by-fasciola-hepatica-implications-of-glycans-and-mucins-for-vaccine-development-2157-7560-5-233.php?aid=28636
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS Publlishing group
publisher.none.fl_str_mv OMICS Publlishing group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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