Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers

Autores
Ubillos, Lluis; Freire, Teresa; Berriel, Edgardo; Chiribao, María Laura; Chiale, Carolina; Festari, María Florencia; Medeiros, Andrea; Mazal, Daniel; Rondan, Mariella; Bollati Fogolin, Mariela; Rabinovich, Gabriel Adrián; Robello, Carlos; Osinaga, Eduardo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, has anticancer effects mediated, at least in part, by parasite-derived products which inhibit growth of tumor cells. We investigated whether immunity to T. cruzi antigens could induce antitumor activity, using two rat models which reproduce human carcinogenesis: colon cancer induced by 1,2-dimethylhydrazine (DMH), and mammary cancer induced by N-nitroso-N-methylurea (NMU). We found that vaccination with T. cruzi epimastigote lysates strongly inhibits tumor development in both animal models. Rats immunized with T. cruzi antigens induce activation of both CD4(+) and CD8(+) T cells and splenocytes from these animals showed higher cytotoxic responses against tumors as compared to rats receiving adjuvant alone. Tumor-associated immune responses included increasing number of CD11b/c(+) His48(-) MHC II(+) cells corresponding to macrophages and/or dendritic cells, which exhibited augmented NADPH-oxidase activity. We also found that T. cruzi lysate vaccination developed antibodies specific for colon and mammary rat cancer cells, which were capable of mediating antibody-dependent cellular cytotoxicity (ADCC) in vitro. Anti-T. cruzi antibodies cross-reacted with human colon and breast cancer cell lines and recognized 41/60 (68%) colon cancer and 38/63 (60%) breast cancer samples in a series of 123 human tumors. Our results suggest that T. cruzi antigens can evoke an integrated antitumor response involving both the cellular and humoral components of the immune response and provide novel insights into the understanding of the intricate relationship between parasite infection and tumor growth
Fil: Ubillos, Lluis. Universidad de la República; Uruguay
Fil: Freire, Teresa. Universidad de la República; Uruguay
Fil: Berriel, Edgardo. Instituto Pasteur de Montevideo; Uruguay. Universidad de la República; Uruguay
Fil: Chiribao, María Laura. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Chiale, Carolina. Universidad de la República; Uruguay
Fil: Festari, María Florencia. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Medeiros, Andrea. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Mazal, Daniel. Universidad de la República; Uruguay
Fil: Rondan, Mariella. Universidad de la República; Uruguay
Fil: Bollati Fogolin, Mariela. Instituto Pasteur de Montevideo; Uruguay
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Robello, Carlos. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Osinaga, Eduardo. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Materia
Trypanosoma Cruzi
Immunology
Cancer
Breast Neoplasm
Colonic Neoplasms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23363
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23363
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancersUbillos, LluisFreire, TeresaBerriel, EdgardoChiribao, María LauraChiale, CarolinaFestari, María FlorenciaMedeiros, AndreaMazal, DanielRondan, MariellaBollati Fogolin, MarielaRabinovich, Gabriel AdriánRobello, CarlosOsinaga, EduardoTrypanosoma CruziImmunologyCancerBreast NeoplasmColonic Neoplasmshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, has anticancer effects mediated, at least in part, by parasite-derived products which inhibit growth of tumor cells. We investigated whether immunity to T. cruzi antigens could induce antitumor activity, using two rat models which reproduce human carcinogenesis: colon cancer induced by 1,2-dimethylhydrazine (DMH), and mammary cancer induced by N-nitroso-N-methylurea (NMU). We found that vaccination with T. cruzi epimastigote lysates strongly inhibits tumor development in both animal models. Rats immunized with T. cruzi antigens induce activation of both CD4(+) and CD8(+) T cells and splenocytes from these animals showed higher cytotoxic responses against tumors as compared to rats receiving adjuvant alone. Tumor-associated immune responses included increasing number of CD11b/c(+) His48(-) MHC II(+) cells corresponding to macrophages and/or dendritic cells, which exhibited augmented NADPH-oxidase activity. We also found that T. cruzi lysate vaccination developed antibodies specific for colon and mammary rat cancer cells, which were capable of mediating antibody-dependent cellular cytotoxicity (ADCC) in vitro. Anti-T. cruzi antibodies cross-reacted with human colon and breast cancer cell lines and recognized 41/60 (68%) colon cancer and 38/63 (60%) breast cancer samples in a series of 123 human tumors. Our results suggest that T. cruzi antigens can evoke an integrated antitumor response involving both the cellular and humoral components of the immune response and provide novel insights into the understanding of the intricate relationship between parasite infection and tumor growthFil: Ubillos, Lluis. Universidad de la República; UruguayFil: Freire, Teresa. Universidad de la República; UruguayFil: Berriel, Edgardo. Instituto Pasteur de Montevideo; Uruguay. Universidad de la República; UruguayFil: Chiribao, María Laura. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; UruguayFil: Chiale, Carolina. Universidad de la República; UruguayFil: Festari, María Florencia. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; UruguayFil: Medeiros, Andrea. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; UruguayFil: Mazal, Daniel. Universidad de la República; UruguayFil: Rondan, Mariella. Universidad de la República; UruguayFil: Bollati Fogolin, Mariela. Instituto Pasteur de Montevideo; UruguayFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Robello, Carlos. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; UruguayFil: Osinaga, Eduardo. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; UruguayJohn Wiley & Sons Inc2016-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/23363Ubillos, Lluis; Freire, Teresa; Berriel, Edgardo; Chiribao, María Laura; Chiale, Carolina; et al.; Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers; John Wiley & Sons Inc; International Journal Of Cancer. Journal International Du Cancer.; 138; 7; 1-4-2016; 1719-17310020-71361097-0215CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/ijc.29910/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/ijc.29910info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:39:34Zoai:ri.conicet.gov.ar:11336/23363instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:39:35.06CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers
title Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers
spellingShingle Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers
Ubillos, Lluis
Trypanosoma Cruzi
Immunology
Cancer
Breast Neoplasm
Colonic Neoplasms
title_short Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers
title_full Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers
title_fullStr Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers
title_full_unstemmed Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers
title_sort Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers
dc.creator.none.fl_str_mv Ubillos, Lluis
Freire, Teresa
Berriel, Edgardo
Chiribao, María Laura
Chiale, Carolina
Festari, María Florencia
Medeiros, Andrea
Mazal, Daniel
Rondan, Mariella
Bollati Fogolin, Mariela
Rabinovich, Gabriel Adrián
Robello, Carlos
Osinaga, Eduardo
author Ubillos, Lluis
author_facet Ubillos, Lluis
Freire, Teresa
Berriel, Edgardo
Chiribao, María Laura
Chiale, Carolina
Festari, María Florencia
Medeiros, Andrea
Mazal, Daniel
Rondan, Mariella
Bollati Fogolin, Mariela
Rabinovich, Gabriel Adrián
Robello, Carlos
Osinaga, Eduardo
author_role author
author2 Freire, Teresa
Berriel, Edgardo
Chiribao, María Laura
Chiale, Carolina
Festari, María Florencia
Medeiros, Andrea
Mazal, Daniel
Rondan, Mariella
Bollati Fogolin, Mariela
Rabinovich, Gabriel Adrián
Robello, Carlos
Osinaga, Eduardo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma Cruzi
Immunology
Cancer
Breast Neoplasm
Colonic Neoplasms
topic Trypanosoma Cruzi
Immunology
Cancer
Breast Neoplasm
Colonic Neoplasms
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, has anticancer effects mediated, at least in part, by parasite-derived products which inhibit growth of tumor cells. We investigated whether immunity to T. cruzi antigens could induce antitumor activity, using two rat models which reproduce human carcinogenesis: colon cancer induced by 1,2-dimethylhydrazine (DMH), and mammary cancer induced by N-nitroso-N-methylurea (NMU). We found that vaccination with T. cruzi epimastigote lysates strongly inhibits tumor development in both animal models. Rats immunized with T. cruzi antigens induce activation of both CD4(+) and CD8(+) T cells and splenocytes from these animals showed higher cytotoxic responses against tumors as compared to rats receiving adjuvant alone. Tumor-associated immune responses included increasing number of CD11b/c(+) His48(-) MHC II(+) cells corresponding to macrophages and/or dendritic cells, which exhibited augmented NADPH-oxidase activity. We also found that T. cruzi lysate vaccination developed antibodies specific for colon and mammary rat cancer cells, which were capable of mediating antibody-dependent cellular cytotoxicity (ADCC) in vitro. Anti-T. cruzi antibodies cross-reacted with human colon and breast cancer cell lines and recognized 41/60 (68%) colon cancer and 38/63 (60%) breast cancer samples in a series of 123 human tumors. Our results suggest that T. cruzi antigens can evoke an integrated antitumor response involving both the cellular and humoral components of the immune response and provide novel insights into the understanding of the intricate relationship between parasite infection and tumor growth
Fil: Ubillos, Lluis. Universidad de la República; Uruguay
Fil: Freire, Teresa. Universidad de la República; Uruguay
Fil: Berriel, Edgardo. Instituto Pasteur de Montevideo; Uruguay. Universidad de la República; Uruguay
Fil: Chiribao, María Laura. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Chiale, Carolina. Universidad de la República; Uruguay
Fil: Festari, María Florencia. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Medeiros, Andrea. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Mazal, Daniel. Universidad de la República; Uruguay
Fil: Rondan, Mariella. Universidad de la República; Uruguay
Fil: Bollati Fogolin, Mariela. Instituto Pasteur de Montevideo; Uruguay
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Robello, Carlos. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Osinaga, Eduardo. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
description Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, has anticancer effects mediated, at least in part, by parasite-derived products which inhibit growth of tumor cells. We investigated whether immunity to T. cruzi antigens could induce antitumor activity, using two rat models which reproduce human carcinogenesis: colon cancer induced by 1,2-dimethylhydrazine (DMH), and mammary cancer induced by N-nitroso-N-methylurea (NMU). We found that vaccination with T. cruzi epimastigote lysates strongly inhibits tumor development in both animal models. Rats immunized with T. cruzi antigens induce activation of both CD4(+) and CD8(+) T cells and splenocytes from these animals showed higher cytotoxic responses against tumors as compared to rats receiving adjuvant alone. Tumor-associated immune responses included increasing number of CD11b/c(+) His48(-) MHC II(+) cells corresponding to macrophages and/or dendritic cells, which exhibited augmented NADPH-oxidase activity. We also found that T. cruzi lysate vaccination developed antibodies specific for colon and mammary rat cancer cells, which were capable of mediating antibody-dependent cellular cytotoxicity (ADCC) in vitro. Anti-T. cruzi antibodies cross-reacted with human colon and breast cancer cell lines and recognized 41/60 (68%) colon cancer and 38/63 (60%) breast cancer samples in a series of 123 human tumors. Our results suggest that T. cruzi antigens can evoke an integrated antitumor response involving both the cellular and humoral components of the immune response and provide novel insights into the understanding of the intricate relationship between parasite infection and tumor growth
publishDate 2016
dc.date.none.fl_str_mv 2016-04-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23363
Ubillos, Lluis; Freire, Teresa; Berriel, Edgardo; Chiribao, María Laura; Chiale, Carolina; et al.; Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers; John Wiley & Sons Inc; International Journal Of Cancer. Journal International Du Cancer.; 138; 7; 1-4-2016; 1719-1731
0020-7136
1097-0215
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23363
identifier_str_mv Ubillos, Lluis; Freire, Teresa; Berriel, Edgardo; Chiribao, María Laura; Chiale, Carolina; et al.; Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers; John Wiley & Sons Inc; International Journal Of Cancer. Journal International Du Cancer.; 138; 7; 1-4-2016; 1719-1731
0020-7136
1097-0215
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/ijc.29910/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/ijc.29910
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc
publisher.none.fl_str_mv John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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