Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
- Autores
- Quintana, Verónica Mara; Torres, Nicolás; Wachsman, Mónica B.; Sinko, Patrick J.; Castilla, Viviana; Chikindas, Michael
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the present study we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. We determined, using a virus yield inhibition assay, that non cytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h post-infection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin treated culture. On the other hand, at high concentrations subtilosin displays virucidal action.
Fil: Quintana, Verónica Mara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Torres, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Wachsman, Mónica B.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Sinko, Patrick J.. State University of New Jersey; Estados Unidos
Fil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Chikindas, Michael. State University of New Jersey; Estados Unidos - Materia
-
BACTERIOCIN
HERPES SIMPLEX 2
ANTIVIRAL ACTIVITY
MICROBICIDE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/26283
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Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosinQuintana, Verónica MaraTorres, NicolásWachsman, Mónica B.Sinko, Patrick J.Castilla, VivianaChikindas, MichaelBACTERIOCINHERPES SIMPLEX 2ANTIVIRAL ACTIVITYMICROBICIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present study we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. We determined, using a virus yield inhibition assay, that non cytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h post-infection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin treated culture. On the other hand, at high concentrations subtilosin displays virucidal action.Fil: Quintana, Verónica Mara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Torres, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Wachsman, Mónica B.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Sinko, Patrick J.. State University of New Jersey; Estados UnidosFil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Chikindas, Michael. State University of New Jersey; Estados UnidosWiley Blackwell Publishing, Inc2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26283Quintana, Verónica Mara; Torres, Nicolás; Wachsman, Mónica B.; Sinko, Patrick J.; Castilla, Viviana; et al.; Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 117; 5; 11-2014; 1253-12591364-50721365-2672CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12618/abstractinfo:eu-repo/semantics/altIdentifier/doi/ 10.1111/jam.12618info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198449/info:eu-repo/semantics/altIdentifier/doi/ 25087911info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:50Zoai:ri.conicet.gov.ar:11336/26283instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:50.719CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin |
title |
Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin |
spellingShingle |
Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin Quintana, Verónica Mara BACTERIOCIN HERPES SIMPLEX 2 ANTIVIRAL ACTIVITY MICROBICIDE |
title_short |
Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin |
title_full |
Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin |
title_fullStr |
Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin |
title_full_unstemmed |
Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin |
title_sort |
Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin |
dc.creator.none.fl_str_mv |
Quintana, Verónica Mara Torres, Nicolás Wachsman, Mónica B. Sinko, Patrick J. Castilla, Viviana Chikindas, Michael |
author |
Quintana, Verónica Mara |
author_facet |
Quintana, Verónica Mara Torres, Nicolás Wachsman, Mónica B. Sinko, Patrick J. Castilla, Viviana Chikindas, Michael |
author_role |
author |
author2 |
Torres, Nicolás Wachsman, Mónica B. Sinko, Patrick J. Castilla, Viviana Chikindas, Michael |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
BACTERIOCIN HERPES SIMPLEX 2 ANTIVIRAL ACTIVITY MICROBICIDE |
topic |
BACTERIOCIN HERPES SIMPLEX 2 ANTIVIRAL ACTIVITY MICROBICIDE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In the present study we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. We determined, using a virus yield inhibition assay, that non cytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h post-infection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin treated culture. On the other hand, at high concentrations subtilosin displays virucidal action. Fil: Quintana, Verónica Mara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina Fil: Torres, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Wachsman, Mónica B.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina Fil: Sinko, Patrick J.. State University of New Jersey; Estados Unidos Fil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina Fil: Chikindas, Michael. State University of New Jersey; Estados Unidos |
description |
In the present study we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. We determined, using a virus yield inhibition assay, that non cytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h post-infection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin treated culture. On the other hand, at high concentrations subtilosin displays virucidal action. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/26283 Quintana, Verónica Mara; Torres, Nicolás; Wachsman, Mónica B.; Sinko, Patrick J.; Castilla, Viviana; et al.; Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 117; 5; 11-2014; 1253-1259 1364-5072 1365-2672 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/26283 |
identifier_str_mv |
Quintana, Verónica Mara; Torres, Nicolás; Wachsman, Mónica B.; Sinko, Patrick J.; Castilla, Viviana; et al.; Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 117; 5; 11-2014; 1253-1259 1364-5072 1365-2672 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12618/abstract info:eu-repo/semantics/altIdentifier/doi/ 10.1111/jam.12618 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198449/ info:eu-repo/semantics/altIdentifier/doi/ 25087911 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613410701967360 |
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13.070432 |