Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin

Autores
Quintana, Verónica Mara; Torres, Nicolás; Wachsman, Mónica B.; Sinko, Patrick J.; Castilla, Viviana; Chikindas, Michael
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. We determined, using a virus yield inhibition assay, that non cytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h post-infection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin treated culture. On the other hand, at high concentrations subtilosin displays virucidal action.
Fil: Quintana, Verónica Mara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Torres, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Wachsman, Mónica B.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Sinko, Patrick J.. State University of New Jersey; Estados Unidos
Fil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Chikindas, Michael. State University of New Jersey; Estados Unidos
Materia
BACTERIOCIN
HERPES SIMPLEX 2
ANTIVIRAL ACTIVITY
MICROBICIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26283

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spelling Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosinQuintana, Verónica MaraTorres, NicolásWachsman, Mónica B.Sinko, Patrick J.Castilla, VivianaChikindas, MichaelBACTERIOCINHERPES SIMPLEX 2ANTIVIRAL ACTIVITYMICROBICIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present study we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. We determined, using a virus yield inhibition assay, that non cytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h post-infection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin treated culture. On the other hand, at high concentrations subtilosin displays virucidal action.Fil: Quintana, Verónica Mara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Torres, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Wachsman, Mónica B.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Sinko, Patrick J.. State University of New Jersey; Estados UnidosFil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Chikindas, Michael. State University of New Jersey; Estados UnidosWiley Blackwell Publishing, Inc2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26283Quintana, Verónica Mara; Torres, Nicolás; Wachsman, Mónica B.; Sinko, Patrick J.; Castilla, Viviana; et al.; Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 117; 5; 11-2014; 1253-12591364-50721365-2672CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12618/abstractinfo:eu-repo/semantics/altIdentifier/doi/ 10.1111/jam.12618info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198449/info:eu-repo/semantics/altIdentifier/doi/ 25087911info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:50Zoai:ri.conicet.gov.ar:11336/26283instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:50.719CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
spellingShingle Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
Quintana, Verónica Mara
BACTERIOCIN
HERPES SIMPLEX 2
ANTIVIRAL ACTIVITY
MICROBICIDE
title_short Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_full Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_fullStr Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_full_unstemmed Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_sort Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
dc.creator.none.fl_str_mv Quintana, Verónica Mara
Torres, Nicolás
Wachsman, Mónica B.
Sinko, Patrick J.
Castilla, Viviana
Chikindas, Michael
author Quintana, Verónica Mara
author_facet Quintana, Verónica Mara
Torres, Nicolás
Wachsman, Mónica B.
Sinko, Patrick J.
Castilla, Viviana
Chikindas, Michael
author_role author
author2 Torres, Nicolás
Wachsman, Mónica B.
Sinko, Patrick J.
Castilla, Viviana
Chikindas, Michael
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIOCIN
HERPES SIMPLEX 2
ANTIVIRAL ACTIVITY
MICROBICIDE
topic BACTERIOCIN
HERPES SIMPLEX 2
ANTIVIRAL ACTIVITY
MICROBICIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present study we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. We determined, using a virus yield inhibition assay, that non cytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h post-infection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin treated culture. On the other hand, at high concentrations subtilosin displays virucidal action.
Fil: Quintana, Verónica Mara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Torres, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Wachsman, Mónica B.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Sinko, Patrick J.. State University of New Jersey; Estados Unidos
Fil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Chikindas, Michael. State University of New Jersey; Estados Unidos
description In the present study we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. We determined, using a virus yield inhibition assay, that non cytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h post-infection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin treated culture. On the other hand, at high concentrations subtilosin displays virucidal action.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26283
Quintana, Verónica Mara; Torres, Nicolás; Wachsman, Mónica B.; Sinko, Patrick J.; Castilla, Viviana; et al.; Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 117; 5; 11-2014; 1253-1259
1364-5072
1365-2672
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26283
identifier_str_mv Quintana, Verónica Mara; Torres, Nicolás; Wachsman, Mónica B.; Sinko, Patrick J.; Castilla, Viviana; et al.; Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 117; 5; 11-2014; 1253-1259
1364-5072
1365-2672
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12618/abstract
info:eu-repo/semantics/altIdentifier/doi/ 10.1111/jam.12618
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198449/
info:eu-repo/semantics/altIdentifier/doi/ 25087911
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
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instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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